Experiment: Thallium(I) acetate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3309 and Shew_3310 overlap by 4 nucleotides Shew_3310 and Shew_3311 are separated by 134 nucleotides Shew_3311 and Shew_3312 are separated by 30 nucleotides Shew_3312 and Shew_3313 are separated by 2 nucleotides Shew_3313 and Shew_3314 overlap by 4 nucleotides
Shew_3309: Shew_3309 - OstA family protein (RefSeq), at 3,950,185 to 3,950,730
_3309
Shew_3310: Shew_3310 - ABC transporter-related protein (RefSeq), at 3,950,727 to 3,951,458
_3310
Shew_3311: Shew_3311 - RNA polymerase factor sigma-54 (RefSeq), at 3,951,593 to 3,953,080
_3311
Shew_3312: Shew_3312 - sigma 54 modulation protein/ribosomal protein S30EA (RefSeq), at 3,953,111 to 3,953,398
_3312
Shew_3313: Shew_3313 - PTS IIA-like nitrogen-regulatory protein PtsN (RefSeq), at 3,953,401 to 3,953,844
_3313
Shew_3314: Shew_3314 - hypothetical protein (RefSeq), at 3,953,841 to 3,954,689
_3314
Position (kb)
3951
3952
3953
3954 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3951.933 kb on - strand, within Shew_3311 at 3952.179 kb on - strand, within Shew_3311 at 3952.193 kb on + strand, within Shew_3311 at 3952.738 kb on + strand, within Shew_3311 at 3953.051 kb on - strand at 3953.217 kb on - strand, within Shew_3312 at 3953.569 kb on + strand, within Shew_3313 at 3953.569 kb on + strand, within Shew_3313
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 10 mg/ml remove 3,951,933 - Shew_3311 0.23 +1.7 3,952,179 - Shew_3311 0.39 +2.2 3,952,193 + Shew_3311 0.40 +1.6 3,952,738 + Shew_3311 0.77 +2.0 3,953,051 - +2.5 3,953,217 - Shew_3312 0.37 -2.5 3,953,569 + Shew_3313 0.38 -2.6 3,953,569 + Shew_3313 0.38 -3.1
Or see this region's nucleotide sequence