Experiment: Thallium(I) acetate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2389 and Shew_2390 are separated by 564 nucleotides Shew_2390 and pflA are separated by 103 nucleotides pflA and Shew_2392 are separated by 6 nucleotides
Shew_2389: Shew_2389 - hypothetical protein (RefSeq), at 2,800,399 to 2,801,931
_2389
Shew_2390: Shew_2390 - formate acetyltransferase (RefSeq), at 2,802,496 to 2,804,778
_2390
Shew_2391: pflA - pyruvate formate lyase-activating enzyme 1 (RefSeq), at 2,804,882 to 2,805,622
pflA
Shew_2392: Shew_2392 - hypothetical protein (RefSeq), at 2,805,629 to 2,806,072
_2392
Position (kb)
2802
2803
2804
2805 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2801.587 kb on + strand, within Shew_2389 at 2801.595 kb on - strand, within Shew_2389 at 2801.667 kb on + strand, within Shew_2389 at 2801.768 kb on - strand, within Shew_2389 at 2801.980 kb on + strand at 2801.980 kb on + strand at 2801.988 kb on - strand at 2801.988 kb on - strand at 2802.323 kb on + strand at 2802.956 kb on + strand, within Shew_2390 at 2802.964 kb on - strand, within Shew_2390 at 2802.998 kb on - strand, within Shew_2390 at 2803.176 kb on + strand, within Shew_2390 at 2803.481 kb on - strand, within Shew_2390 at 2803.608 kb on - strand, within Shew_2390 at 2803.625 kb on - strand, within Shew_2390 at 2803.682 kb on + strand, within Shew_2390 at 2803.985 kb on - strand, within Shew_2390 at 2804.127 kb on + strand, within Shew_2390 at 2804.127 kb on + strand, within Shew_2390 at 2804.495 kb on - strand, within Shew_2390 at 2804.495 kb on - strand, within Shew_2390 at 2804.551 kb on - strand at 2804.663 kb on - strand at 2804.720 kb on - strand at 2805.149 kb on + strand, within pflA at 2805.154 kb on + strand, within pflA at 2805.162 kb on - strand, within pflA at 2805.162 kb on - strand, within pflA at 2805.183 kb on + strand, within pflA
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 10 mg/ml remove 2,801,587 + Shew_2389 0.77 +2.2 2,801,595 - Shew_2389 0.78 -0.1 2,801,667 + Shew_2389 0.83 -0.3 2,801,768 - Shew_2389 0.89 -1.7 2,801,980 + -0.2 2,801,980 + +1.2 2,801,988 - -0.7 2,801,988 - +0.3 2,802,323 + +0.8 2,802,956 + Shew_2390 0.20 -1.2 2,802,964 - Shew_2390 0.20 +1.7 2,802,998 - Shew_2390 0.22 -0.2 2,803,176 + Shew_2390 0.30 -1.0 2,803,481 - Shew_2390 0.43 -0.3 2,803,608 - Shew_2390 0.49 -0.7 2,803,625 - Shew_2390 0.49 -1.3 2,803,682 + Shew_2390 0.52 -0.4 2,803,985 - Shew_2390 0.65 -2.4 2,804,127 + Shew_2390 0.71 +0.5 2,804,127 + Shew_2390 0.71 +0.6 2,804,495 - Shew_2390 0.88 -0.6 2,804,495 - Shew_2390 0.88 -1.0 2,804,551 - -0.7 2,804,663 - -0.5 2,804,720 - -1.9 2,805,149 + pflA Shew_2391 0.36 -1.6 2,805,154 + pflA Shew_2391 0.37 -1.1 2,805,162 - pflA Shew_2391 0.38 -1.7 2,805,162 - pflA Shew_2391 0.38 +0.1 2,805,183 + pflA Shew_2391 0.41 -2.8
Or see this region's nucleotide sequence