Experiment: Thallium(I) acetate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1937 and Shew_1938 are separated by 9 nucleotides Shew_1938 and Shew_1939 overlap by 4 nucleotides Shew_1939 and ansA are separated by 59 nucleotides ansA and Shew_1941 are separated by 121 nucleotides
Shew_1937: Shew_1937 - peptidase M14, carboxypeptidase A (RefSeq), at 2,237,876 to 2,239,003
_1937
Shew_1938: Shew_1938 - hypothetical protein (RefSeq), at 2,239,013 to 2,239,330
_1938
Shew_1939: Shew_1939 - hypothetical protein (RefSeq), at 2,239,327 to 2,239,608
_1939
Shew_1940: ansA - cytoplasmic asparaginase I (RefSeq), at 2,239,668 to 2,240,681
ansA
Shew_1941: Shew_1941 - signal peptide peptidase SppA, 67K type (RefSeq), at 2,240,803 to 2,242,644
_1941
Position (kb)
2239
2240
2241 Strain fitness (log2 ratio)
-2
-1
0
1 at 2238.804 kb on + strand, within Shew_1937 at 2239.547 kb on - strand, within Shew_1939 at 2239.726 kb on + strand at 2240.566 kb on + strand, within ansA at 2240.871 kb on - strand at 2241.031 kb on - strand, within Shew_1941 at 2241.098 kb on - strand, within Shew_1941 at 2241.585 kb on + strand, within Shew_1941
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 10 mg/ml remove 2,238,804 + Shew_1937 0.82 -0.2 2,239,547 - Shew_1939 0.78 +1.3 2,239,726 + -2.4 2,240,566 + ansA Shew_1940 0.89 -1.4 2,240,871 - -0.2 2,241,031 - Shew_1941 0.12 -0.5 2,241,098 - Shew_1941 0.16 -1.4 2,241,585 + Shew_1941 0.42 +1.3
Or see this region's nucleotide sequence