Experiment: Thallium(I) acetate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1668 and Shew_1669 are separated by 162 nucleotides Shew_1669 and Shew_1670 are separated by 15 nucleotides Shew_1670 and Shew_1671 overlap by 1 nucleotides
Shew_1668: Shew_1668 - methylmalonate-semialdehyde dehydrogenase (RefSeq), at 1,917,266 to 1,918,771
_1668
Shew_1669: Shew_1669 - butyryl-CoA dehydrogenase (RefSeq), at 1,918,934 to 1,920,091
_1669
Shew_1670: Shew_1670 - enoyl-CoA hydratase (RefSeq), at 1,920,107 to 1,920,880
_1670
Shew_1671: Shew_1671 - enoyl-CoA hydratase/isomerase (RefSeq), at 1,920,880 to 1,922,010
_1671
Position (kb)
1918
1919
1920
1921 Strain fitness (log2 ratio)
-1
0
1
2 at 1918.118 kb on - strand, within Shew_1668 at 1918.279 kb on + strand, within Shew_1668 at 1918.287 kb on - strand, within Shew_1668 at 1918.287 kb on - strand, within Shew_1668 at 1919.184 kb on + strand, within Shew_1669 at 1919.291 kb on + strand, within Shew_1669 at 1919.299 kb on - strand, within Shew_1669 at 1919.352 kb on - strand, within Shew_1669 at 1919.371 kb on - strand, within Shew_1669 at 1919.681 kb on - strand, within Shew_1669 at 1919.720 kb on - strand, within Shew_1669 at 1920.249 kb on + strand, within Shew_1670 at 1920.249 kb on + strand, within Shew_1670 at 1920.533 kb on + strand, within Shew_1670 at 1920.541 kb on - strand, within Shew_1670 at 1920.627 kb on - strand, within Shew_1670 at 1920.876 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 10 mg/ml remove 1,918,118 - Shew_1668 0.57 +1.1 1,918,279 + Shew_1668 0.67 +0.2 1,918,287 - Shew_1668 0.68 +0.9 1,918,287 - Shew_1668 0.68 +0.9 1,919,184 + Shew_1669 0.22 +0.7 1,919,291 + Shew_1669 0.31 +0.8 1,919,299 - Shew_1669 0.32 +0.8 1,919,352 - Shew_1669 0.36 +0.2 1,919,371 - Shew_1669 0.38 +0.3 1,919,681 - Shew_1669 0.65 +0.8 1,919,720 - Shew_1669 0.68 +2.1 1,920,249 + Shew_1670 0.18 +1.0 1,920,249 + Shew_1670 0.18 +0.1 1,920,533 + Shew_1670 0.55 +0.3 1,920,541 - Shew_1670 0.56 +0.8 1,920,627 - Shew_1670 0.67 -0.2 1,920,876 + +0.2
Or see this region's nucleotide sequence