Experiment: Thallium(I) acetate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cyaA and Shew_0320 are separated by 7 nucleotides Shew_0320 and Shew_0321 are separated by 489 nucleotides Shew_0321 and Shew_0322 are separated by 5 nucleotides
Shew_0319: cyaA - adenylate cyclase (RefSeq), at 364,802 to 367,222
cyaA
Shew_0320: Shew_0320 - frataxin family protein (RefSeq), at 367,230 to 367,604
_0320
Shew_0321: Shew_0321 - hypothetical protein (RefSeq), at 368,094 to 368,261
_0321
Shew_0322: Shew_0322 - diaminopimelate decarboxylase (RefSeq), at 368,267 to 369,511
_0322
Position (kb) 367
368 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 366.356 kb on - strand, within cyaA at 366.595 kb on + strand, within cyaA at 366.603 kb on - strand, within cyaA at 367.053 kb on + strand at 367.262 kb on + strand at 367.262 kb on + strand at 367.270 kb on - strand, within Shew_0320 at 367.270 kb on - strand, within Shew_0320 at 367.270 kb on - strand, within Shew_0320 at 367.313 kb on - strand, within Shew_0320 at 367.413 kb on - strand, within Shew_0320 at 368.416 kb on - strand, within Shew_0322 at 368.423 kb on + strand, within Shew_0322 at 368.449 kb on - strand, within Shew_0322 at 368.451 kb on + strand, within Shew_0322 at 368.459 kb on - strand, within Shew_0322 at 368.459 kb on - strand, within Shew_0322
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 10 mg/ml remove 366,356 - cyaA Shew_0319 0.64 +0.4 366,595 + cyaA Shew_0319 0.74 +0.5 366,603 - cyaA Shew_0319 0.74 +0.3 367,053 + +0.4 367,262 + -4.7 367,262 + -3.2 367,270 - Shew_0320 0.11 -4.0 367,270 - Shew_0320 0.11 -2.3 367,270 - Shew_0320 0.11 -6.3 367,313 - Shew_0320 0.22 -5.3 367,413 - Shew_0320 0.49 -5.2 368,416 - Shew_0322 0.12 -0.1 368,423 + Shew_0322 0.13 +1.0 368,449 - Shew_0322 0.15 +1.4 368,451 + Shew_0322 0.15 +1.3 368,459 - Shew_0322 0.15 +0.1 368,459 - Shew_0322 0.15 +2.8
Or see this region's nucleotide sequence