Experiment: Thallium(I) acetate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0289 and ilvM overlap by 4 nucleotides ilvM and Shew_0291 are separated by 26 nucleotides Shew_0291 and Shew_0292 are separated by 112 nucleotides
Shew_0289: Shew_0289 - acetolactate synthase 2 catalytic subunit (RefSeq), at 308,543 to 310,216
_0289
Shew_0290: ilvM - acetolactate synthase 2 regulatory subunit (RefSeq), at 310,213 to 310,482
ilvM
Shew_0291: Shew_0291 - branched-chain amino acid aminotransferase (RefSeq), at 310,509 to 311,432
_0291
Shew_0292: Shew_0292 - dihydroxy-acid dehydratase (RefSeq), at 311,545 to 313,395
_0292
Position (kb) 310
311
312 Strain fitness (log2 ratio) -1
0
1 at 309.543 kb on + strand, within Shew_0289 at 309.697 kb on + strand, within Shew_0289 at 309.730 kb on + strand, within Shew_0289 at 309.763 kb on - strand, within Shew_0289 at 309.820 kb on - strand, within Shew_0289 at 310.036 kb on - strand, within Shew_0289 at 310.141 kb on + strand at 310.300 kb on - strand, within ilvM at 310.347 kb on + strand, within ilvM at 310.655 kb on + strand, within Shew_0291 at 311.098 kb on + strand, within Shew_0291 at 311.106 kb on - strand, within Shew_0291 at 311.129 kb on + strand, within Shew_0291 at 311.137 kb on - strand, within Shew_0291 at 311.137 kb on - strand, within Shew_0291 at 311.137 kb on - strand, within Shew_0291 at 311.137 kb on - strand, within Shew_0291 at 311.424 kb on - strand at 311.424 kb on - strand at 311.459 kb on + strand at 311.669 kb on + strand at 311.763 kb on + strand, within Shew_0292 at 311.814 kb on + strand, within Shew_0292 at 311.814 kb on + strand, within Shew_0292 at 311.831 kb on + strand, within Shew_0292 at 311.934 kb on - strand, within Shew_0292 at 312.076 kb on + strand, within Shew_0292 at 312.166 kb on + strand, within Shew_0292 at 312.326 kb on + strand, within Shew_0292 at 312.334 kb on - strand, within Shew_0292
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 10 mg/ml remove 309,543 + Shew_0289 0.60 +0.3 309,697 + Shew_0289 0.69 +0.2 309,730 + Shew_0289 0.71 +0.2 309,763 - Shew_0289 0.73 +0.1 309,820 - Shew_0289 0.76 -1.2 310,036 - Shew_0289 0.89 -0.9 310,141 + +1.0 310,300 - ilvM Shew_0290 0.32 +0.6 310,347 + ilvM Shew_0290 0.50 +0.0 310,655 + Shew_0291 0.16 +0.9 311,098 + Shew_0291 0.64 +0.2 311,106 - Shew_0291 0.65 +0.4 311,129 + Shew_0291 0.67 +0.1 311,137 - Shew_0291 0.68 -1.2 311,137 - Shew_0291 0.68 -1.2 311,137 - Shew_0291 0.68 +0.1 311,137 - Shew_0291 0.68 -0.3 311,424 - +0.7 311,424 - -0.4 311,459 + -0.0 311,669 + +0.4 311,763 + Shew_0292 0.12 -0.1 311,814 + Shew_0292 0.15 -0.4 311,814 + Shew_0292 0.15 +1.3 311,831 + Shew_0292 0.15 +0.3 311,934 - Shew_0292 0.21 +0.5 312,076 + Shew_0292 0.29 +1.7 312,166 + Shew_0292 0.34 -0.5 312,326 + Shew_0292 0.42 -1.0 312,334 - Shew_0292 0.43 -1.6
Or see this region's nucleotide sequence