Experiment: Thallium(I) acetate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0016 and Shew_0017 overlap by 17 nucleotides Shew_0017 and fadA are separated by 354 nucleotides fadA and fadB are separated by 21 nucleotides
Shew_0016: Shew_0016 - ybaK/ebsC protein (RefSeq), at 18,563 to 19,027
_0016
Shew_0017: Shew_0017 - GCN5-related N-acetyltransferase (RefSeq), at 19,011 to 19,499
_0017
Shew_0018: fadA - 3-ketoacyl-CoA thiolase (RefSeq), at 19,854 to 21,017
fadA
Shew_0019: fadB - multifunctional fatty acid oxidation complex subunit alpha (RefSeq), at 21,039 to 23,189
fadB
Position (kb) 19
20
21
22 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 19.024 kb on + strand at 19.075 kb on + strand, within Shew_0017 at 19.170 kb on + strand, within Shew_0017 at 19.334 kb on + strand, within Shew_0017 at 19.339 kb on + strand, within Shew_0017 at 19.346 kb on + strand, within Shew_0017 at 19.347 kb on - strand, within Shew_0017 at 19.426 kb on + strand, within Shew_0017 at 19.771 kb on - strand at 19.871 kb on - strand at 19.900 kb on - strand at 19.960 kb on - strand at 19.961 kb on + strand at 19.965 kb on - strand at 20.088 kb on + strand, within fadA at 20.162 kb on + strand, within fadA at 20.297 kb on + strand, within fadA at 20.660 kb on - strand, within fadA at 20.836 kb on - strand, within fadA at 20.916 kb on - strand at 20.981 kb on - strand at 21.160 kb on - strand at 21.160 kb on - strand at 21.192 kb on - strand at 21.216 kb on - strand at 21.369 kb on + strand, within fadB at 21.417 kb on - strand, within fadB at 21.457 kb on + strand, within fadB at 21.544 kb on + strand, within fadB at 21.557 kb on - strand, within fadB at 21.784 kb on - strand, within fadB at 21.855 kb on - strand, within fadB at 21.870 kb on - strand, within fadB at 21.882 kb on - strand, within fadB
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 10 mg/ml remove 19,024 + -0.0 19,075 + Shew_0017 0.13 -1.1 19,170 + Shew_0017 0.33 -1.6 19,334 + Shew_0017 0.66 +0.6 19,339 + Shew_0017 0.67 +0.2 19,346 + Shew_0017 0.69 -0.4 19,347 - Shew_0017 0.69 -0.1 19,426 + Shew_0017 0.85 +0.8 19,771 - -0.8 19,871 - -0.7 19,900 - +0.2 19,960 - +1.0 19,961 + -0.2 19,965 - +0.0 20,088 + fadA Shew_0018 0.20 -3.1 20,162 + fadA Shew_0018 0.26 -0.5 20,297 + fadA Shew_0018 0.38 -1.9 20,660 - fadA Shew_0018 0.69 +0.3 20,836 - fadA Shew_0018 0.84 -0.1 20,916 - -1.1 20,981 - -0.2 21,160 - -1.5 21,160 - -2.2 21,192 - -1.3 21,216 - -0.5 21,369 + fadB Shew_0019 0.15 -1.6 21,417 - fadB Shew_0019 0.18 -0.8 21,457 + fadB Shew_0019 0.19 -0.8 21,544 + fadB Shew_0019 0.23 -1.8 21,557 - fadB Shew_0019 0.24 -1.7 21,784 - fadB Shew_0019 0.35 -0.9 21,855 - fadB Shew_0019 0.38 -1.5 21,870 - fadB Shew_0019 0.39 -1.0 21,882 - fadB Shew_0019 0.39 +1.8
Or see this region's nucleotide sequence