Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuD and Synpcc7942_2549 are separated by 72 nucleotides Synpcc7942_2549 and Synpcc7942_2550 are separated by 128 nucleotides Synpcc7942_2550 and hypF are separated by 189 nucleotides
Synpcc7942_2548: leuD - isopropylmalate isomerase small subunit, at 2,627,423 to 2,628,031
leuD
Synpcc7942_2549: Synpcc7942_2549 - hypothetical protein, at 2,628,104 to 2,628,358
_2549
Synpcc7942_2550: Synpcc7942_2550 - hypothetical protein, at 2,628,487 to 2,629,560
_2550
Synpcc7942_2551: hypF - (NiFe) hydrogenase maturation protein HypF, at 2,629,750 to 2,632,062
hypF
Position (kb)
2628
2629
2630 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2628.051 kb on + strand at 2628.051 kb on + strand at 2628.051 kb on + strand at 2628.051 kb on + strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.059 kb on - strand at 2628.085 kb on + strand at 2628.090 kb on + strand at 2628.102 kb on + strand at 2628.108 kb on + strand at 2628.187 kb on - strand, within Synpcc7942_2549 at 2628.209 kb on - strand, within Synpcc7942_2549 at 2628.212 kb on + strand, within Synpcc7942_2549 at 2628.222 kb on + strand, within Synpcc7942_2549 at 2628.234 kb on + strand, within Synpcc7942_2549 at 2628.267 kb on + strand, within Synpcc7942_2549 at 2628.284 kb on + strand, within Synpcc7942_2549 at 2628.343 kb on + strand at 2628.343 kb on + strand at 2628.343 kb on + strand at 2628.343 kb on + strand at 2628.343 kb on + strand at 2628.343 kb on + strand at 2628.351 kb on - strand at 2628.351 kb on - strand at 2628.351 kb on - strand at 2628.351 kb on - strand at 2628.351 kb on - strand at 2628.373 kb on + strand at 2628.373 kb on + strand at 2628.391 kb on - strand at 2628.397 kb on + strand at 2628.397 kb on + strand at 2628.405 kb on - strand at 2628.416 kb on + strand at 2628.429 kb on - strand at 2628.433 kb on + strand at 2628.433 kb on + strand at 2628.441 kb on - strand at 2628.441 kb on - strand at 2628.441 kb on - strand at 2628.483 kb on + strand at 2628.500 kb on + strand at 2628.520 kb on + strand at 2628.556 kb on + strand at 2628.564 kb on - strand at 2628.564 kb on - strand at 2628.564 kb on - strand at 2628.566 kb on + strand at 2628.566 kb on + strand at 2628.574 kb on - strand at 2628.574 kb on - strand at 2628.574 kb on - strand at 2628.576 kb on + strand at 2628.617 kb on + strand, within Synpcc7942_2550 at 2628.621 kb on - strand, within Synpcc7942_2550 at 2628.622 kb on + strand, within Synpcc7942_2550 at 2628.622 kb on - strand, within Synpcc7942_2550 at 2628.627 kb on + strand, within Synpcc7942_2550 at 2628.641 kb on + strand, within Synpcc7942_2550 at 2628.641 kb on + strand, within Synpcc7942_2550 at 2628.641 kb on - strand, within Synpcc7942_2550 at 2628.649 kb on - strand, within Synpcc7942_2550 at 2628.716 kb on + strand, within Synpcc7942_2550 at 2628.724 kb on - strand, within Synpcc7942_2550 at 2628.726 kb on + strand, within Synpcc7942_2550 at 2628.771 kb on + strand, within Synpcc7942_2550 at 2628.799 kb on + strand, within Synpcc7942_2550 at 2628.805 kb on - strand, within Synpcc7942_2550 at 2628.805 kb on - strand, within Synpcc7942_2550 at 2628.871 kb on + strand, within Synpcc7942_2550 at 2628.888 kb on + strand, within Synpcc7942_2550 at 2628.910 kb on - strand, within Synpcc7942_2550 at 2628.922 kb on - strand, within Synpcc7942_2550 at 2628.943 kb on - strand, within Synpcc7942_2550 at 2628.946 kb on + strand, within Synpcc7942_2550 at 2628.946 kb on + strand, within Synpcc7942_2550 at 2628.953 kb on - strand at 2628.954 kb on - strand, within Synpcc7942_2550 at 2628.956 kb on - strand, within Synpcc7942_2550 at 2628.969 kb on + strand, within Synpcc7942_2550 at 2628.982 kb on + strand, within Synpcc7942_2550 at 2628.988 kb on - strand, within Synpcc7942_2550 at 2629.014 kb on + strand, within Synpcc7942_2550 at 2629.056 kb on - strand, within Synpcc7942_2550 at 2629.061 kb on + strand, within Synpcc7942_2550 at 2629.069 kb on - strand, within Synpcc7942_2550 at 2629.069 kb on - strand, within Synpcc7942_2550 at 2629.084 kb on + strand, within Synpcc7942_2550 at 2629.151 kb on + strand, within Synpcc7942_2550 at 2629.151 kb on + strand, within Synpcc7942_2550 at 2629.152 kb on + strand at 2629.159 kb on - strand, within Synpcc7942_2550 at 2629.159 kb on - strand, within Synpcc7942_2550 at 2629.159 kb on - strand, within Synpcc7942_2550 at 2629.160 kb on - strand, within Synpcc7942_2550 at 2629.184 kb on - strand, within Synpcc7942_2550 at 2629.189 kb on + strand, within Synpcc7942_2550 at 2629.189 kb on + strand, within Synpcc7942_2550 at 2629.189 kb on + strand, within Synpcc7942_2550 at 2629.189 kb on + strand, within Synpcc7942_2550 at 2629.189 kb on + strand, within Synpcc7942_2550 at 2629.189 kb on + strand, within Synpcc7942_2550 at 2629.197 kb on - strand, within Synpcc7942_2550 at 2629.197 kb on - strand, within Synpcc7942_2550 at 2629.197 kb on - strand, within Synpcc7942_2550 at 2629.197 kb on - strand, within Synpcc7942_2550 at 2629.197 kb on - strand, within Synpcc7942_2550 at 2629.217 kb on - strand, within Synpcc7942_2550 at 2629.225 kb on - strand, within Synpcc7942_2550 at 2629.245 kb on + strand, within Synpcc7942_2550 at 2629.253 kb on - strand, within Synpcc7942_2550 at 2629.370 kb on + strand, within Synpcc7942_2550 at 2629.389 kb on - strand, within Synpcc7942_2550 at 2629.390 kb on - strand, within Synpcc7942_2550 at 2629.404 kb on - strand, within Synpcc7942_2550 at 2629.413 kb on - strand, within Synpcc7942_2550 at 2629.437 kb on + strand, within Synpcc7942_2550 at 2629.445 kb on - strand, within Synpcc7942_2550 at 2629.456 kb on + strand at 2629.456 kb on + strand at 2629.456 kb on + strand at 2629.456 kb on + strand at 2629.464 kb on - strand at 2629.464 kb on - strand at 2629.466 kb on + strand at 2629.474 kb on - strand at 2629.480 kb on + strand at 2629.480 kb on + strand at 2629.485 kb on + strand at 2629.488 kb on - strand at 2629.493 kb on + strand at 2629.504 kb on + strand at 2629.546 kb on - strand at 2629.555 kb on + strand at 2629.568 kb on - strand at 2629.601 kb on - strand at 2629.611 kb on - strand at 2629.637 kb on - strand at 2629.637 kb on - strand at 2629.682 kb on + strand at 2629.682 kb on + strand at 2629.709 kb on + strand at 2629.716 kb on + strand at 2629.717 kb on - strand at 2629.741 kb on - strand at 2629.742 kb on - strand at 2629.755 kb on - strand at 2629.758 kb on - strand at 2629.771 kb on - strand at 2629.792 kb on + strand at 2629.798 kb on - strand at 2629.811 kb on - strand at 2629.829 kb on + strand at 2629.829 kb on + strand at 2629.829 kb on + strand at 2629.829 kb on + strand at 2629.829 kb on + strand at 2629.834 kb on + strand at 2629.837 kb on - strand at 2629.837 kb on - strand at 2629.837 kb on - strand at 2629.837 kb on - strand at 2629.837 kb on - strand at 2629.906 kb on + strand at 2629.933 kb on - strand at 2629.974 kb on + strand at 2629.975 kb on + strand at 2629.975 kb on + strand at 2629.983 kb on - strand, within hypF at 2629.983 kb on - strand, within hypF at 2629.983 kb on - strand, within hypF at 2629.983 kb on - strand, within hypF at 2629.983 kb on - strand, within hypF at 2629.983 kb on - strand, within hypF at 2629.983 kb on - strand, within hypF at 2630.006 kb on - strand, within hypF at 2630.048 kb on + strand, within hypF at 2630.053 kb on + strand, within hypF at 2630.053 kb on + strand, within hypF at 2630.061 kb on - strand, within hypF at 2630.112 kb on - strand, within hypF at 2630.154 kb on - strand, within hypF at 2630.197 kb on - strand, within hypF at 2630.211 kb on + strand, within hypF at 2630.246 kb on - strand at 2630.293 kb on - strand, within hypF at 2630.335 kb on - strand, within hypF at 2630.345 kb on + strand, within hypF at 2630.368 kb on - strand, within hypF at 2630.374 kb on + strand, within hypF at 2630.374 kb on + strand, within hypF at 2630.385 kb on + strand, within hypF at 2630.388 kb on - strand, within hypF at 2630.388 kb on - strand, within hypF at 2630.388 kb on - strand, within hypF at 2630.420 kb on + strand, within hypF at 2630.420 kb on + strand, within hypF at 2630.422 kb on + strand, within hypF at 2630.428 kb on - strand, within hypF at 2630.428 kb on - strand, within hypF at 2630.430 kb on - strand, within hypF at 2630.437 kb on + strand, within hypF at 2630.445 kb on - strand, within hypF at 2630.449 kb on + strand, within hypF at 2630.451 kb on - strand, within hypF at 2630.459 kb on + strand, within hypF at 2630.465 kb on + strand, within hypF at 2630.487 kb on - strand at 2630.492 kb on - strand, within hypF at 2630.529 kb on - strand, within hypF at 2630.531 kb on + strand, within hypF
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,628,051 + -1.6 2,628,051 + -0.6 2,628,051 + +3.6 2,628,051 + +2.8 2,628,059 - -0.7 2,628,059 - +1.5 2,628,059 - -1.3 2,628,059 - +0.7 2,628,059 - +0.4 2,628,059 - -0.0 2,628,085 + +1.5 2,628,090 + -0.8 2,628,102 + +0.6 2,628,108 + +4.4 2,628,187 - Synpcc7942_2549 0.33 -3.6 2,628,209 - Synpcc7942_2549 0.41 +0.8 2,628,212 + Synpcc7942_2549 0.42 -1.1 2,628,222 + Synpcc7942_2549 0.46 +1.6 2,628,234 + Synpcc7942_2549 0.51 -2.6 2,628,267 + Synpcc7942_2549 0.64 -0.8 2,628,284 + Synpcc7942_2549 0.71 -0.8 2,628,343 + +0.9 2,628,343 + -1.2 2,628,343 + +2.0 2,628,343 + -1.4 2,628,343 + +2.0 2,628,343 + -2.3 2,628,351 - -1.0 2,628,351 - +1.5 2,628,351 - +4.4 2,628,351 - +3.2 2,628,351 - -0.2 2,628,373 + -0.1 2,628,373 + -1.4 2,628,391 - +1.3 2,628,397 + -0.8 2,628,397 + -0.8 2,628,405 - -2.3 2,628,416 + +2.1 2,628,429 - +3.3 2,628,433 + +2.0 2,628,433 + -2.0 2,628,441 - -0.5 2,628,441 - -0.9 2,628,441 - -0.7 2,628,483 + -2.2 2,628,500 + +4.2 2,628,520 + -1.9 2,628,556 + +1.0 2,628,564 - +2.6 2,628,564 - +1.1 2,628,564 - +2.6 2,628,566 + +1.9 2,628,566 + +1.9 2,628,574 - -0.0 2,628,574 - +0.5 2,628,574 - +0.5 2,628,576 + -0.2 2,628,617 + Synpcc7942_2550 0.12 +4.0 2,628,621 - Synpcc7942_2550 0.12 +1.6 2,628,622 + Synpcc7942_2550 0.13 +1.8 2,628,622 - Synpcc7942_2550 0.13 +0.9 2,628,627 + Synpcc7942_2550 0.13 +4.2 2,628,641 + Synpcc7942_2550 0.14 +1.5 2,628,641 + Synpcc7942_2550 0.14 +0.8 2,628,641 - Synpcc7942_2550 0.14 +1.4 2,628,649 - Synpcc7942_2550 0.15 +2.8 2,628,716 + Synpcc7942_2550 0.21 +3.4 2,628,724 - Synpcc7942_2550 0.22 +1.6 2,628,726 + Synpcc7942_2550 0.22 -0.1 2,628,771 + Synpcc7942_2550 0.26 -0.2 2,628,799 + Synpcc7942_2550 0.29 +1.4 2,628,805 - Synpcc7942_2550 0.30 +4.5 2,628,805 - Synpcc7942_2550 0.30 +2.5 2,628,871 + Synpcc7942_2550 0.36 +4.0 2,628,888 + Synpcc7942_2550 0.37 +2.5 2,628,910 - Synpcc7942_2550 0.39 +1.3 2,628,922 - Synpcc7942_2550 0.41 +0.4 2,628,943 - Synpcc7942_2550 0.42 -0.5 2,628,946 + Synpcc7942_2550 0.43 +2.8 2,628,946 + Synpcc7942_2550 0.43 +1.3 2,628,953 - -0.0 2,628,954 - Synpcc7942_2550 0.43 +2.7 2,628,956 - Synpcc7942_2550 0.44 +1.7 2,628,969 + Synpcc7942_2550 0.45 +3.1 2,628,982 + Synpcc7942_2550 0.46 +0.7 2,628,988 - Synpcc7942_2550 0.47 +3.0 2,629,014 + Synpcc7942_2550 0.49 -0.3 2,629,056 - Synpcc7942_2550 0.53 +2.0 2,629,061 + Synpcc7942_2550 0.53 -0.6 2,629,069 - Synpcc7942_2550 0.54 +1.7 2,629,069 - Synpcc7942_2550 0.54 +1.4 2,629,084 + Synpcc7942_2550 0.56 +3.5 2,629,151 + Synpcc7942_2550 0.62 +0.6 2,629,151 + Synpcc7942_2550 0.62 +1.0 2,629,152 + +2.4 2,629,159 - Synpcc7942_2550 0.63 +1.9 2,629,159 - Synpcc7942_2550 0.63 +2.7 2,629,159 - Synpcc7942_2550 0.63 +1.5 2,629,160 - Synpcc7942_2550 0.63 -1.5 2,629,184 - Synpcc7942_2550 0.65 +3.2 2,629,189 + Synpcc7942_2550 0.65 +1.5 2,629,189 + Synpcc7942_2550 0.65 +2.9 2,629,189 + Synpcc7942_2550 0.65 +0.4 2,629,189 + Synpcc7942_2550 0.65 +3.9 2,629,189 + Synpcc7942_2550 0.65 +1.2 2,629,189 + Synpcc7942_2550 0.65 +0.1 2,629,197 - Synpcc7942_2550 0.66 +3.0 2,629,197 - Synpcc7942_2550 0.66 +2.0 2,629,197 - Synpcc7942_2550 0.66 +2.8 2,629,197 - Synpcc7942_2550 0.66 +3.9 2,629,197 - Synpcc7942_2550 0.66 +4.2 2,629,217 - Synpcc7942_2550 0.68 +3.8 2,629,225 - Synpcc7942_2550 0.69 +1.2 2,629,245 + Synpcc7942_2550 0.71 +0.4 2,629,253 - Synpcc7942_2550 0.71 +1.5 2,629,370 + Synpcc7942_2550 0.82 +3.2 2,629,389 - Synpcc7942_2550 0.84 +0.5 2,629,390 - Synpcc7942_2550 0.84 +0.2 2,629,404 - Synpcc7942_2550 0.85 +1.8 2,629,413 - Synpcc7942_2550 0.86 +2.0 2,629,437 + Synpcc7942_2550 0.88 +1.6 2,629,445 - Synpcc7942_2550 0.89 +1.8 2,629,456 + +4.2 2,629,456 + +1.9 2,629,456 + +5.1 2,629,456 + +2.4 2,629,464 - +1.7 2,629,464 - +4.6 2,629,466 + +2.0 2,629,474 - +1.5 2,629,480 + -0.2 2,629,480 + +5.1 2,629,485 + +2.9 2,629,488 - +1.3 2,629,493 + +0.4 2,629,504 + +0.6 2,629,546 - +2.0 2,629,555 + -0.5 2,629,568 - -1.8 2,629,601 - -0.3 2,629,611 - +1.5 2,629,637 - +0.1 2,629,637 - -2.1 2,629,682 + -2.5 2,629,682 + +1.5 2,629,709 + -2.9 2,629,716 + +0.3 2,629,717 - -0.6 2,629,741 - +0.9 2,629,742 - -1.9 2,629,755 - +3.2 2,629,758 - -0.2 2,629,771 - -1.9 2,629,792 + +0.0 2,629,798 - +3.2 2,629,811 - -1.0 2,629,829 + -1.5 2,629,829 + -0.4 2,629,829 + -0.6 2,629,829 + -1.8 2,629,829 + -0.1 2,629,834 + -1.8 2,629,837 - -0.6 2,629,837 - -2.2 2,629,837 - +2.3 2,629,837 - +2.0 2,629,837 - +1.8 2,629,906 + -1.4 2,629,933 - +2.1 2,629,974 + -1.2 2,629,975 + +4.0 2,629,975 + -3.0 2,629,983 - hypF Synpcc7942_2551 0.10 +1.1 2,629,983 - hypF Synpcc7942_2551 0.10 +1.4 2,629,983 - hypF Synpcc7942_2551 0.10 +1.7 2,629,983 - hypF Synpcc7942_2551 0.10 -0.4 2,629,983 - hypF Synpcc7942_2551 0.10 +0.3 2,629,983 - hypF Synpcc7942_2551 0.10 +0.7 2,629,983 - hypF Synpcc7942_2551 0.10 +0.2 2,630,006 - hypF Synpcc7942_2551 0.11 -0.6 2,630,048 + hypF Synpcc7942_2551 0.13 -3.4 2,630,053 + hypF Synpcc7942_2551 0.13 +0.3 2,630,053 + hypF Synpcc7942_2551 0.13 +0.1 2,630,061 - hypF Synpcc7942_2551 0.13 +0.6 2,630,112 - hypF Synpcc7942_2551 0.16 -1.6 2,630,154 - hypF Synpcc7942_2551 0.17 -1.0 2,630,197 - hypF Synpcc7942_2551 0.19 +1.0 2,630,211 + hypF Synpcc7942_2551 0.20 +1.6 2,630,246 - -0.0 2,630,293 - hypF Synpcc7942_2551 0.23 +0.9 2,630,335 - hypF Synpcc7942_2551 0.25 +1.1 2,630,345 + hypF Synpcc7942_2551 0.26 -2.8 2,630,368 - hypF Synpcc7942_2551 0.27 +2.7 2,630,374 + hypF Synpcc7942_2551 0.27 +0.1 2,630,374 + hypF Synpcc7942_2551 0.27 -2.6 2,630,385 + hypF Synpcc7942_2551 0.27 -1.9 2,630,388 - hypF Synpcc7942_2551 0.28 -1.2 2,630,388 - hypF Synpcc7942_2551 0.28 -1.0 2,630,388 - hypF Synpcc7942_2551 0.28 -0.7 2,630,420 + hypF Synpcc7942_2551 0.29 -0.7 2,630,420 + hypF Synpcc7942_2551 0.29 +1.0 2,630,422 + hypF Synpcc7942_2551 0.29 -0.8 2,630,428 - hypF Synpcc7942_2551 0.29 -2.7 2,630,428 - hypF Synpcc7942_2551 0.29 -2.7 2,630,430 - hypF Synpcc7942_2551 0.29 +0.4 2,630,437 + hypF Synpcc7942_2551 0.30 -3.4 2,630,445 - hypF Synpcc7942_2551 0.30 +2.7 2,630,449 + hypF Synpcc7942_2551 0.30 -1.5 2,630,451 - hypF Synpcc7942_2551 0.30 -1.6 2,630,459 + hypF Synpcc7942_2551 0.31 +2.5 2,630,465 + hypF Synpcc7942_2551 0.31 -0.3 2,630,487 - -0.6 2,630,492 - hypF Synpcc7942_2551 0.32 -1.6 2,630,529 - hypF Synpcc7942_2551 0.34 -0.5 2,630,531 + hypF Synpcc7942_2551 0.34 -0.4
Or see this region's nucleotide sequence