Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2515 and tRNA-Ala are separated by 124 nucleotides tRNA-Ala and Synpcc7942_2516 are separated by 62 nucleotides Synpcc7942_2516 and Synpcc7942_2517 are separated by 72 nucleotides Synpcc7942_2517 and glgA are separated by 21 nucleotides
Synpcc7942_2515: Synpcc7942_2515 - putative DNA helicase, at 2,594,192 to 2,596,459
_2515
Synpcc7942_R0018: tRNA-Ala - tRNA-Ala, at 2,596,584 to 2,596,656
tRNA-Ala
Synpcc7942_2516: Synpcc7942_2516 - hypothetical protein, at 2,596,719 to 2,596,958
_2516
Synpcc7942_2517: Synpcc7942_2517 - hypothetical protein, at 2,597,031 to 2,597,729
_2517
Synpcc7942_2518: glgA - glycogen synthase, at 2,597,751 to 2,599,148
glgA
Position (kb)
2597
2598 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2596.065 kb on - strand, within Synpcc7942_2515 at 2596.088 kb on + strand, within Synpcc7942_2515 at 2596.103 kb on - strand, within Synpcc7942_2515 at 2596.112 kb on - strand, within Synpcc7942_2515 at 2596.122 kb on - strand, within Synpcc7942_2515 at 2596.125 kb on + strand, within Synpcc7942_2515 at 2596.151 kb on + strand, within Synpcc7942_2515 at 2596.157 kb on + strand, within Synpcc7942_2515 at 2596.158 kb on - strand, within Synpcc7942_2515 at 2596.159 kb on - strand, within Synpcc7942_2515 at 2596.159 kb on - strand, within Synpcc7942_2515 at 2596.165 kb on - strand, within Synpcc7942_2515 at 2596.174 kb on - strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.225 kb on + strand, within Synpcc7942_2515 at 2596.233 kb on - strand at 2596.233 kb on - strand at 2596.233 kb on - strand at 2596.233 kb on - strand at 2596.233 kb on - strand at 2596.233 kb on - strand at 2596.233 kb on - strand at 2596.247 kb on + strand at 2596.257 kb on + strand at 2596.257 kb on - strand at 2596.267 kb on + strand at 2596.291 kb on + strand at 2596.291 kb on + strand at 2596.299 kb on - strand at 2596.299 kb on - strand at 2596.340 kb on + strand at 2596.345 kb on + strand at 2596.348 kb on - strand at 2596.449 kb on - strand at 2596.483 kb on - strand at 2596.485 kb on + strand at 2596.485 kb on - strand at 2596.485 kb on - strand at 2596.503 kb on - strand at 2596.517 kb on - strand at 2596.522 kb on - strand at 2596.594 kb on - strand, within tRNA-Ala at 2596.728 kb on + strand at 2596.823 kb on + strand, within Synpcc7942_2516 at 2596.834 kb on + strand, within Synpcc7942_2516 at 2596.862 kb on - strand, within Synpcc7942_2516 at 2596.869 kb on + strand, within Synpcc7942_2516 at 2596.887 kb on - strand, within Synpcc7942_2516 at 2596.901 kb on + strand, within Synpcc7942_2516 at 2596.982 kb on - strand at 2596.985 kb on + strand at 2596.986 kb on + strand at 2596.993 kb on - strand at 2596.993 kb on - strand at 2596.993 kb on - strand at 2596.993 kb on - strand at 2597.001 kb on + strand at 2597.001 kb on - strand at 2597.008 kb on + strand at 2597.008 kb on + strand at 2597.008 kb on + strand at 2597.013 kb on + strand at 2597.018 kb on + strand at 2597.021 kb on - strand at 2597.077 kb on + strand at 2597.141 kb on - strand, within Synpcc7942_2517 at 2597.144 kb on + strand, within Synpcc7942_2517 at 2597.183 kb on + strand, within Synpcc7942_2517 at 2597.183 kb on + strand, within Synpcc7942_2517 at 2597.216 kb on + strand, within Synpcc7942_2517 at 2597.272 kb on - strand, within Synpcc7942_2517 at 2597.287 kb on + strand, within Synpcc7942_2517 at 2597.427 kb on + strand, within Synpcc7942_2517 at 2597.454 kb on + strand at 2597.459 kb on + strand, within Synpcc7942_2517 at 2597.467 kb on - strand, within Synpcc7942_2517 at 2597.478 kb on + strand, within Synpcc7942_2517 at 2597.535 kb on + strand, within Synpcc7942_2517 at 2597.535 kb on + strand, within Synpcc7942_2517 at 2597.535 kb on + strand, within Synpcc7942_2517 at 2597.543 kb on - strand, within Synpcc7942_2517 at 2597.543 kb on - strand, within Synpcc7942_2517 at 2597.554 kb on + strand, within Synpcc7942_2517 at 2597.558 kb on - strand, within Synpcc7942_2517 at 2597.599 kb on - strand, within Synpcc7942_2517 at 2597.724 kb on + strand at 2597.732 kb on - strand at 2597.732 kb on - strand at 2597.748 kb on + strand at 2597.762 kb on + strand at 2597.778 kb on - strand at 2597.783 kb on - strand at 2597.794 kb on + strand at 2597.794 kb on + strand at 2597.794 kb on + strand at 2597.794 kb on + strand at 2597.794 kb on + strand at 2597.794 kb on + strand at 2597.795 kb on - strand at 2597.802 kb on - strand at 2597.811 kb on + strand at 2597.916 kb on + strand, within glgA at 2598.471 kb on + strand, within glgA at 2598.589 kb on + strand, within glgA at 2598.707 kb on - strand, within glgA
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,596,065 - Synpcc7942_2515 0.83 +1.6 2,596,088 + Synpcc7942_2515 0.84 +0.1 2,596,103 - Synpcc7942_2515 0.84 +1.3 2,596,112 - Synpcc7942_2515 0.85 -1.2 2,596,122 - Synpcc7942_2515 0.85 +4.0 2,596,125 + Synpcc7942_2515 0.85 -1.7 2,596,151 + Synpcc7942_2515 0.86 +2.0 2,596,157 + Synpcc7942_2515 0.87 -1.2 2,596,158 - Synpcc7942_2515 0.87 -2.6 2,596,159 - Synpcc7942_2515 0.87 +0.1 2,596,159 - Synpcc7942_2515 0.87 +2.6 2,596,165 - Synpcc7942_2515 0.87 +2.8 2,596,174 - Synpcc7942_2515 0.87 -0.4 2,596,225 + Synpcc7942_2515 0.90 -2.0 2,596,225 + Synpcc7942_2515 0.90 -0.3 2,596,225 + Synpcc7942_2515 0.90 +1.8 2,596,225 + Synpcc7942_2515 0.90 -0.8 2,596,225 + Synpcc7942_2515 0.90 +0.8 2,596,225 + Synpcc7942_2515 0.90 +0.0 2,596,225 + Synpcc7942_2515 0.90 -1.6 2,596,225 + Synpcc7942_2515 0.90 -2.0 2,596,225 + Synpcc7942_2515 0.90 -2.1 2,596,233 - +1.5 2,596,233 - +0.7 2,596,233 - -0.4 2,596,233 - +0.4 2,596,233 - -0.4 2,596,233 - +0.0 2,596,233 - -1.8 2,596,247 + +2.5 2,596,257 + +3.8 2,596,257 - -0.6 2,596,267 + +1.6 2,596,291 + -0.8 2,596,291 + -1.0 2,596,299 - +0.0 2,596,299 - +2.1 2,596,340 + +1.2 2,596,345 + +2.4 2,596,348 - -1.3 2,596,449 - +1.0 2,596,483 - -0.4 2,596,485 + -2.4 2,596,485 - -1.9 2,596,485 - +0.5 2,596,503 - -2.8 2,596,517 - +2.3 2,596,522 - -1.4 2,596,594 - tRNA-Ala Synpcc7942_R0018 0.14 -0.4 2,596,728 + -1.2 2,596,823 + Synpcc7942_2516 0.43 -1.1 2,596,834 + Synpcc7942_2516 0.48 -1.5 2,596,862 - Synpcc7942_2516 0.60 -4.8 2,596,869 + Synpcc7942_2516 0.62 -2.7 2,596,887 - Synpcc7942_2516 0.70 -2.7 2,596,901 + Synpcc7942_2516 0.76 -1.0 2,596,982 - +1.0 2,596,985 + -1.4 2,596,986 + -1.0 2,596,993 - -3.6 2,596,993 - -1.9 2,596,993 - -2.8 2,596,993 - +0.6 2,597,001 + +1.7 2,597,001 - -0.9 2,597,008 + -1.2 2,597,008 + -0.8 2,597,008 + +0.7 2,597,013 + +0.2 2,597,018 + -1.0 2,597,021 - -1.7 2,597,077 + -3.3 2,597,141 - Synpcc7942_2517 0.16 -2.3 2,597,144 + Synpcc7942_2517 0.16 +0.4 2,597,183 + Synpcc7942_2517 0.22 +0.2 2,597,183 + Synpcc7942_2517 0.22 -3.0 2,597,216 + Synpcc7942_2517 0.26 -3.8 2,597,272 - Synpcc7942_2517 0.34 -3.1 2,597,287 + Synpcc7942_2517 0.37 -1.8 2,597,427 + Synpcc7942_2517 0.57 -1.7 2,597,454 + -1.2 2,597,459 + Synpcc7942_2517 0.61 -2.4 2,597,467 - Synpcc7942_2517 0.62 -1.9 2,597,478 + Synpcc7942_2517 0.64 -3.2 2,597,535 + Synpcc7942_2517 0.72 -3.4 2,597,535 + Synpcc7942_2517 0.72 -2.7 2,597,535 + Synpcc7942_2517 0.72 -3.4 2,597,543 - Synpcc7942_2517 0.73 -3.7 2,597,543 - Synpcc7942_2517 0.73 -1.2 2,597,554 + Synpcc7942_2517 0.75 -1.2 2,597,558 - Synpcc7942_2517 0.75 -1.9 2,597,599 - Synpcc7942_2517 0.81 -3.5 2,597,724 + +0.9 2,597,732 - -0.3 2,597,732 - +1.0 2,597,748 + -1.2 2,597,762 + -1.5 2,597,778 - +0.9 2,597,783 - -2.7 2,597,794 + -2.6 2,597,794 + -2.8 2,597,794 + -2.6 2,597,794 + -2.3 2,597,794 + -3.8 2,597,794 + -1.8 2,597,795 - -2.9 2,597,802 - -0.6 2,597,811 + -1.6 2,597,916 + glgA Synpcc7942_2518 0.12 -0.8 2,598,471 + glgA Synpcc7942_2518 0.52 -0.0 2,598,589 + glgA Synpcc7942_2518 0.60 -0.7 2,598,707 - glgA Synpcc7942_2518 0.68 -2.1
Or see this region's nucleotide sequence