Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2469 and Synpcc7942_2470 are separated by 222 nucleotides Synpcc7942_2470 and nusB are separated by 146 nucleotides nusB and ffh are separated by 14 nucleotides
Synpcc7942_2469: Synpcc7942_2469 - hypothetical protein, at 2,550,704 to 2,551,474
_2469
Synpcc7942_2470: Synpcc7942_2470 - hypothetical protein, at 2,551,697 to 2,552,464
_2470
Synpcc7942_2471: nusB - transcription antitermination protein NusB, at 2,552,611 to 2,553,252
nusB
Synpcc7942_2472: ffh - signal recognition particle-docking protein FtsY, at 2,553,267 to 2,554,823
ffh
Position (kb)
2551
2552
2553 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2550.716 kb on - strand at 2550.738 kb on - strand at 2550.754 kb on - strand at 2550.754 kb on - strand at 2550.768 kb on + strand at 2550.777 kb on + strand at 2550.813 kb on + strand, within Synpcc7942_2469 at 2550.813 kb on + strand, within Synpcc7942_2469 at 2550.816 kb on - strand, within Synpcc7942_2469 at 2550.832 kb on + strand, within Synpcc7942_2469 at 2550.853 kb on - strand, within Synpcc7942_2469 at 2550.909 kb on - strand, within Synpcc7942_2469 at 2550.982 kb on + strand, within Synpcc7942_2469 at 2550.991 kb on - strand, within Synpcc7942_2469 at 2551.044 kb on - strand, within Synpcc7942_2469 at 2551.046 kb on - strand, within Synpcc7942_2469 at 2551.082 kb on - strand, within Synpcc7942_2469 at 2551.095 kb on - strand, within Synpcc7942_2469 at 2551.114 kb on - strand, within Synpcc7942_2469 at 2551.173 kb on + strand, within Synpcc7942_2469 at 2551.173 kb on - strand, within Synpcc7942_2469 at 2551.181 kb on - strand, within Synpcc7942_2469 at 2551.181 kb on - strand, within Synpcc7942_2469 at 2551.201 kb on - strand, within Synpcc7942_2469 at 2551.280 kb on + strand, within Synpcc7942_2469 at 2551.399 kb on + strand at 2551.399 kb on + strand at 2551.445 kb on + strand at 2551.463 kb on + strand at 2551.471 kb on - strand at 2551.511 kb on - strand at 2551.516 kb on + strand at 2551.564 kb on + strand at 2551.588 kb on + strand at 2551.590 kb on - strand at 2551.607 kb on - strand at 2551.651 kb on + strand at 2551.658 kb on - strand at 2551.659 kb on - strand at 2551.709 kb on + strand at 2551.717 kb on - strand at 2551.736 kb on - strand at 2551.787 kb on + strand, within Synpcc7942_2470 at 2551.794 kb on - strand, within Synpcc7942_2470 at 2551.810 kb on - strand, within Synpcc7942_2470 at 2551.818 kb on + strand, within Synpcc7942_2470 at 2551.818 kb on + strand, within Synpcc7942_2470 at 2551.826 kb on - strand, within Synpcc7942_2470 at 2551.849 kb on + strand, within Synpcc7942_2470 at 2551.888 kb on + strand, within Synpcc7942_2470 at 2551.896 kb on - strand, within Synpcc7942_2470 at 2551.896 kb on - strand, within Synpcc7942_2470 at 2551.898 kb on + strand, within Synpcc7942_2470 at 2551.901 kb on - strand, within Synpcc7942_2470 at 2551.903 kb on + strand, within Synpcc7942_2470 at 2551.911 kb on - strand, within Synpcc7942_2470 at 2551.915 kb on - strand, within Synpcc7942_2470 at 2551.917 kb on + strand, within Synpcc7942_2470 at 2551.917 kb on + strand, within Synpcc7942_2470 at 2551.917 kb on + strand, within Synpcc7942_2470 at 2551.917 kb on + strand, within Synpcc7942_2470 at 2551.923 kb on + strand, within Synpcc7942_2470 at 2551.925 kb on - strand, within Synpcc7942_2470 at 2551.925 kb on - strand, within Synpcc7942_2470 at 2551.936 kb on - strand, within Synpcc7942_2470 at 2551.947 kb on + strand, within Synpcc7942_2470 at 2551.954 kb on - strand, within Synpcc7942_2470 at 2551.973 kb on - strand, within Synpcc7942_2470 at 2551.973 kb on - strand, within Synpcc7942_2470 at 2552.010 kb on - strand, within Synpcc7942_2470 at 2552.079 kb on - strand, within Synpcc7942_2470 at 2552.081 kb on + strand, within Synpcc7942_2470 at 2552.081 kb on + strand, within Synpcc7942_2470 at 2552.081 kb on + strand, within Synpcc7942_2470 at 2552.111 kb on + strand, within Synpcc7942_2470 at 2552.126 kb on - strand, within Synpcc7942_2470 at 2552.142 kb on + strand, within Synpcc7942_2470 at 2552.149 kb on - strand, within Synpcc7942_2470 at 2552.205 kb on + strand, within Synpcc7942_2470 at 2552.223 kb on - strand, within Synpcc7942_2470 at 2552.230 kb on - strand, within Synpcc7942_2470 at 2552.232 kb on - strand, within Synpcc7942_2470 at 2552.232 kb on - strand, within Synpcc7942_2470 at 2552.259 kb on + strand, within Synpcc7942_2470 at 2552.259 kb on + strand, within Synpcc7942_2470 at 2552.259 kb on + strand, within Synpcc7942_2470 at 2552.267 kb on - strand, within Synpcc7942_2470 at 2552.267 kb on - strand, within Synpcc7942_2470 at 2552.267 kb on - strand, within Synpcc7942_2470 at 2552.267 kb on - strand, within Synpcc7942_2470 at 2552.269 kb on + strand, within Synpcc7942_2470 at 2552.269 kb on + strand, within Synpcc7942_2470 at 2552.274 kb on + strand, within Synpcc7942_2470 at 2552.277 kb on - strand, within Synpcc7942_2470 at 2552.282 kb on - strand, within Synpcc7942_2470 at 2552.292 kb on + strand, within Synpcc7942_2470 at 2552.292 kb on + strand, within Synpcc7942_2470 at 2552.292 kb on + strand, within Synpcc7942_2470 at 2552.300 kb on - strand, within Synpcc7942_2470 at 2552.300 kb on - strand, within Synpcc7942_2470 at 2552.304 kb on - strand, within Synpcc7942_2470 at 2552.340 kb on + strand, within Synpcc7942_2470 at 2552.375 kb on + strand, within Synpcc7942_2470 at 2552.375 kb on + strand, within Synpcc7942_2470 at 2552.375 kb on + strand, within Synpcc7942_2470 at 2552.413 kb on + strand at 2552.416 kb on - strand at 2552.421 kb on - strand at 2552.447 kb on + strand at 2552.473 kb on - strand at 2552.497 kb on + strand at 2552.497 kb on + strand at 2552.497 kb on + strand at 2552.499 kb on + strand at 2552.499 kb on + strand at 2552.499 kb on + strand at 2552.499 kb on + strand at 2552.499 kb on + strand at 2552.499 kb on + strand at 2552.507 kb on - strand at 2552.507 kb on - strand at 2552.509 kb on + strand at 2552.509 kb on + strand at 2552.531 kb on + strand at 2552.531 kb on + strand at 2552.539 kb on - strand at 2552.542 kb on + strand at 2552.542 kb on + strand at 2552.552 kb on + strand at 2552.555 kb on - strand at 2552.561 kb on - strand at 2552.564 kb on + strand at 2552.564 kb on + strand at 2552.585 kb on - strand at 2552.834 kb on + strand, within nusB at 2553.209 kb on - strand at 2553.216 kb on + strand at 2553.219 kb on - strand at 2553.219 kb on - strand at 2553.224 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,550,716 - -2.6 2,550,738 - -1.7 2,550,754 - -0.9 2,550,754 - -3.2 2,550,768 + -3.4 2,550,777 + -2.0 2,550,813 + Synpcc7942_2469 0.14 -1.4 2,550,813 + Synpcc7942_2469 0.14 +2.7 2,550,816 - Synpcc7942_2469 0.15 -3.9 2,550,832 + Synpcc7942_2469 0.17 -5.5 2,550,853 - Synpcc7942_2469 0.19 -4.3 2,550,909 - Synpcc7942_2469 0.27 -1.6 2,550,982 + Synpcc7942_2469 0.36 -3.3 2,550,991 - Synpcc7942_2469 0.37 -0.8 2,551,044 - Synpcc7942_2469 0.44 -3.3 2,551,046 - Synpcc7942_2469 0.44 -2.8 2,551,082 - Synpcc7942_2469 0.49 -3.6 2,551,095 - Synpcc7942_2469 0.51 -4.3 2,551,114 - Synpcc7942_2469 0.53 -2.6 2,551,173 + Synpcc7942_2469 0.61 -2.4 2,551,173 - Synpcc7942_2469 0.61 -2.0 2,551,181 - Synpcc7942_2469 0.62 -3.3 2,551,181 - Synpcc7942_2469 0.62 -2.9 2,551,201 - Synpcc7942_2469 0.64 -1.8 2,551,280 + Synpcc7942_2469 0.75 -3.8 2,551,399 + -4.7 2,551,399 + -2.3 2,551,445 + -2.8 2,551,463 + -4.6 2,551,471 - -1.0 2,551,511 - +0.6 2,551,516 + -1.1 2,551,564 + -1.6 2,551,588 + -0.5 2,551,590 - +2.0 2,551,607 - -0.4 2,551,651 + -1.3 2,551,658 - +1.1 2,551,659 - +1.6 2,551,709 + -2.1 2,551,717 - -2.6 2,551,736 - -0.7 2,551,787 + Synpcc7942_2470 0.12 -1.0 2,551,794 - Synpcc7942_2470 0.13 -0.3 2,551,810 - Synpcc7942_2470 0.15 -2.0 2,551,818 + Synpcc7942_2470 0.16 +0.2 2,551,818 + Synpcc7942_2470 0.16 -0.0 2,551,826 - Synpcc7942_2470 0.17 -1.8 2,551,849 + Synpcc7942_2470 0.20 +0.3 2,551,888 + Synpcc7942_2470 0.25 -3.1 2,551,896 - Synpcc7942_2470 0.26 -2.4 2,551,896 - Synpcc7942_2470 0.26 -2.6 2,551,898 + Synpcc7942_2470 0.26 +1.0 2,551,901 - Synpcc7942_2470 0.27 -5.0 2,551,903 + Synpcc7942_2470 0.27 -1.4 2,551,911 - Synpcc7942_2470 0.28 -0.6 2,551,915 - Synpcc7942_2470 0.28 -3.3 2,551,917 + Synpcc7942_2470 0.29 -1.6 2,551,917 + Synpcc7942_2470 0.29 -2.1 2,551,917 + Synpcc7942_2470 0.29 -2.8 2,551,917 + Synpcc7942_2470 0.29 -1.0 2,551,923 + Synpcc7942_2470 0.29 -1.0 2,551,925 - Synpcc7942_2470 0.30 -2.3 2,551,925 - Synpcc7942_2470 0.30 -1.0 2,551,936 - Synpcc7942_2470 0.31 -4.7 2,551,947 + Synpcc7942_2470 0.33 -2.2 2,551,954 - Synpcc7942_2470 0.33 -1.4 2,551,973 - Synpcc7942_2470 0.36 -1.8 2,551,973 - Synpcc7942_2470 0.36 -1.7 2,552,010 - Synpcc7942_2470 0.41 -3.9 2,552,079 - Synpcc7942_2470 0.50 -2.3 2,552,081 + Synpcc7942_2470 0.50 -3.4 2,552,081 + Synpcc7942_2470 0.50 -2.8 2,552,081 + Synpcc7942_2470 0.50 -0.9 2,552,111 + Synpcc7942_2470 0.54 -2.8 2,552,126 - Synpcc7942_2470 0.56 -3.6 2,552,142 + Synpcc7942_2470 0.58 -1.0 2,552,149 - Synpcc7942_2470 0.59 -3.4 2,552,205 + Synpcc7942_2470 0.66 -2.8 2,552,223 - Synpcc7942_2470 0.68 -3.5 2,552,230 - Synpcc7942_2470 0.69 -4.1 2,552,232 - Synpcc7942_2470 0.70 -0.6 2,552,232 - Synpcc7942_2470 0.70 -1.4 2,552,259 + Synpcc7942_2470 0.73 -2.0 2,552,259 + Synpcc7942_2470 0.73 -2.1 2,552,259 + Synpcc7942_2470 0.73 -2.7 2,552,267 - Synpcc7942_2470 0.74 -0.2 2,552,267 - Synpcc7942_2470 0.74 -0.1 2,552,267 - Synpcc7942_2470 0.74 +0.2 2,552,267 - Synpcc7942_2470 0.74 -0.4 2,552,269 + Synpcc7942_2470 0.74 -0.0 2,552,269 + Synpcc7942_2470 0.74 -1.6 2,552,274 + Synpcc7942_2470 0.75 -2.3 2,552,277 - Synpcc7942_2470 0.76 -2.0 2,552,282 - Synpcc7942_2470 0.76 -1.8 2,552,292 + Synpcc7942_2470 0.77 -2.2 2,552,292 + Synpcc7942_2470 0.77 -2.9 2,552,292 + Synpcc7942_2470 0.77 -0.6 2,552,300 - Synpcc7942_2470 0.79 -2.7 2,552,300 - Synpcc7942_2470 0.79 -1.2 2,552,304 - Synpcc7942_2470 0.79 -2.3 2,552,340 + Synpcc7942_2470 0.84 +0.2 2,552,375 + Synpcc7942_2470 0.88 -2.6 2,552,375 + Synpcc7942_2470 0.88 -1.9 2,552,375 + Synpcc7942_2470 0.88 +3.0 2,552,413 + -2.1 2,552,416 - +0.8 2,552,421 - -2.1 2,552,447 + -0.3 2,552,473 - -3.4 2,552,497 + -0.6 2,552,497 + +3.1 2,552,497 + +0.7 2,552,499 + -0.2 2,552,499 + +0.7 2,552,499 + -0.8 2,552,499 + +1.2 2,552,499 + +1.1 2,552,499 + -1.8 2,552,507 - -0.1 2,552,507 - -1.0 2,552,509 + -1.5 2,552,509 + +0.6 2,552,531 + +0.5 2,552,531 + +0.5 2,552,539 - -2.5 2,552,542 + +0.5 2,552,542 + -1.7 2,552,552 + +2.2 2,552,555 - -0.6 2,552,561 - +0.7 2,552,564 + -2.0 2,552,564 + -2.8 2,552,585 - -0.4 2,552,834 + nusB Synpcc7942_2471 0.35 -0.8 2,553,209 - -1.2 2,553,216 + -1.6 2,553,219 - -2.2 2,553,219 - -0.0 2,553,224 - -2.0
Or see this region's nucleotide sequence