Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pstC and pstS are separated by 85 nucleotides pstS and sphX are separated by 279 nucleotides
Synpcc7942_2443: pstC - Phosphate ABC transporter, permease protein PstC, at 2,519,609 to 2,520,541
pstC
Synpcc7942_2444: pstS - Phosphate binding protein, at 2,520,627 to 2,521,640
pstS
Synpcc7942_2445: sphX - Phosphate binding protein, at 2,521,920 to 2,522,933
sphX
Position (kb)
2520
2521
2522 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2520.538 kb on + strand at 2520.538 kb on + strand at 2520.543 kb on + strand at 2520.645 kb on + strand at 2520.656 kb on + strand at 2520.882 kb on - strand, within pstS at 2521.131 kb on - strand, within pstS at 2521.839 kb on + strand at 2521.839 kb on + strand at 2521.839 kb on + strand at 2521.847 kb on - strand at 2521.847 kb on - strand at 2521.847 kb on - strand at 2521.947 kb on + strand at 2522.029 kb on + strand, within sphX at 2522.029 kb on + strand, within sphX at 2522.043 kb on - strand, within sphX at 2522.151 kb on + strand, within sphX at 2522.159 kb on - strand, within sphX at 2522.176 kb on - strand, within sphX at 2522.187 kb on + strand, within sphX at 2522.204 kb on - strand, within sphX at 2522.218 kb on + strand, within sphX at 2522.226 kb on - strand, within sphX at 2522.226 kb on - strand, within sphX at 2522.248 kb on + strand, within sphX at 2522.253 kb on + strand, within sphX at 2522.294 kb on + strand, within sphX at 2522.294 kb on + strand, within sphX at 2522.362 kb on - strand, within sphX at 2522.429 kb on - strand, within sphX at 2522.443 kb on + strand, within sphX at 2522.451 kb on - strand, within sphX at 2522.460 kb on - strand, within sphX at 2522.536 kb on - strand, within sphX at 2522.547 kb on + strand, within sphX at 2522.547 kb on - strand, within sphX
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,520,538 + -1.0 2,520,538 + +1.4 2,520,543 + +1.0 2,520,645 + -3.2 2,520,656 + -2.3 2,520,882 - pstS Synpcc7942_2444 0.25 -3.0 2,521,131 - pstS Synpcc7942_2444 0.50 -3.5 2,521,839 + +1.0 2,521,839 + +0.1 2,521,839 + -0.8 2,521,847 - -0.8 2,521,847 - -0.9 2,521,847 - +0.0 2,521,947 + -1.0 2,522,029 + sphX Synpcc7942_2445 0.11 +0.7 2,522,029 + sphX Synpcc7942_2445 0.11 -0.5 2,522,043 - sphX Synpcc7942_2445 0.12 -0.2 2,522,151 + sphX Synpcc7942_2445 0.23 +4.6 2,522,159 - sphX Synpcc7942_2445 0.24 -1.8 2,522,176 - sphX Synpcc7942_2445 0.25 -2.9 2,522,187 + sphX Synpcc7942_2445 0.26 -3.5 2,522,204 - sphX Synpcc7942_2445 0.28 -2.1 2,522,218 + sphX Synpcc7942_2445 0.29 +1.0 2,522,226 - sphX Synpcc7942_2445 0.30 -1.8 2,522,226 - sphX Synpcc7942_2445 0.30 -0.4 2,522,248 + sphX Synpcc7942_2445 0.32 -0.6 2,522,253 + sphX Synpcc7942_2445 0.33 +1.5 2,522,294 + sphX Synpcc7942_2445 0.37 +1.4 2,522,294 + sphX Synpcc7942_2445 0.37 +1.2 2,522,362 - sphX Synpcc7942_2445 0.44 +0.5 2,522,429 - sphX Synpcc7942_2445 0.50 -0.6 2,522,443 + sphX Synpcc7942_2445 0.52 -1.8 2,522,451 - sphX Synpcc7942_2445 0.52 +3.3 2,522,460 - sphX Synpcc7942_2445 0.53 +0.8 2,522,536 - sphX Synpcc7942_2445 0.61 +1.8 2,522,547 + sphX Synpcc7942_2445 0.62 +0.6 2,522,547 - sphX Synpcc7942_2445 0.62 -0.5
Or see this region's nucleotide sequence