Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acrF and Synpcc7942_2370 are separated by 20 nucleotides Synpcc7942_2370 and lpxA are separated by 10 nucleotides lpxA and Synpcc7942_2372 are separated by 40 nucleotides
Synpcc7942_2369: acrF - Hydrophobe/amphiphile efflux-1 HAE1, at 2,435,041 to 2,438,211
acrF
Synpcc7942_2370: Synpcc7942_2370 - hypothetical protein, at 2,438,232 to 2,438,441
_2370
Synpcc7942_2371: lpxA - UDP-N-acetylglucosamine acyltransferase, at 2,438,452 to 2,439,246
lpxA
Synpcc7942_2372: Synpcc7942_2372 - hypothetical protein, at 2,439,287 to 2,440,336
_2372
Position (kb)
2438
2439
2440 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 2437.487 kb on - strand, within acrF at 2437.499 kb on - strand, within acrF at 2437.499 kb on - strand, within acrF at 2437.564 kb on - strand, within acrF at 2437.564 kb on - strand, within acrF at 2437.624 kb on + strand, within acrF at 2437.672 kb on + strand, within acrF at 2437.681 kb on - strand, within acrF at 2437.796 kb on + strand, within acrF at 2437.826 kb on + strand, within acrF at 2437.834 kb on - strand, within acrF at 2437.841 kb on + strand, within acrF at 2437.917 kb on - strand at 2437.923 kb on + strand at 2437.923 kb on + strand at 2437.925 kb on - strand at 2437.926 kb on - strand at 2437.967 kb on + strand at 2438.023 kb on + strand at 2438.103 kb on - strand at 2438.139 kb on + strand at 2438.178 kb on + strand at 2438.254 kb on + strand, within Synpcc7942_2370 at 2438.259 kb on + strand, within Synpcc7942_2370 at 2438.316 kb on + strand, within Synpcc7942_2370 at 2438.322 kb on + strand, within Synpcc7942_2370 at 2438.348 kb on + strand, within Synpcc7942_2370 at 2438.364 kb on + strand, within Synpcc7942_2370 at 2438.377 kb on - strand, within Synpcc7942_2370 at 2438.377 kb on - strand, within Synpcc7942_2370 at 2438.381 kb on + strand, within Synpcc7942_2370 at 2438.399 kb on - strand, within Synpcc7942_2370 at 2438.400 kb on - strand, within Synpcc7942_2370 at 2438.424 kb on + strand at 2438.449 kb on + strand at 2438.515 kb on + strand at 2438.627 kb on - strand, within lpxA at 2438.696 kb on + strand, within lpxA at 2439.055 kb on + strand, within lpxA at 2439.160 kb on + strand, within lpxA at 2439.253 kb on + strand at 2439.283 kb on - strand at 2439.283 kb on - strand at 2439.284 kb on + strand at 2439.285 kb on + strand at 2439.293 kb on - strand at 2439.293 kb on - strand at 2439.293 kb on - strand at 2439.293 kb on - strand at 2439.293 kb on - strand at 2439.293 kb on - strand at 2439.307 kb on + strand at 2439.309 kb on - strand at 2439.346 kb on + strand at 2439.351 kb on + strand at 2439.354 kb on - strand at 2439.354 kb on - strand at 2439.354 kb on - strand at 2439.354 kb on - strand at 2439.354 kb on - strand at 2439.354 kb on - strand at 2439.359 kb on - strand at 2439.359 kb on - strand at 2439.382 kb on + strand at 2439.408 kb on + strand, within Synpcc7942_2372 at 2439.408 kb on + strand, within Synpcc7942_2372 at 2439.416 kb on - strand, within Synpcc7942_2372 at 2439.439 kb on + strand, within Synpcc7942_2372 at 2439.444 kb on + strand, within Synpcc7942_2372 at 2439.447 kb on - strand, within Synpcc7942_2372 at 2439.452 kb on - strand, within Synpcc7942_2372 at 2439.463 kb on + strand, within Synpcc7942_2372 at 2439.463 kb on - strand, within Synpcc7942_2372 at 2439.494 kb on - strand, within Synpcc7942_2372 at 2439.499 kb on - strand, within Synpcc7942_2372 at 2439.577 kb on + strand, within Synpcc7942_2372 at 2439.599 kb on + strand, within Synpcc7942_2372 at 2439.605 kb on + strand, within Synpcc7942_2372 at 2439.632 kb on + strand, within Synpcc7942_2372 at 2439.649 kb on - strand, within Synpcc7942_2372 at 2439.659 kb on - strand, within Synpcc7942_2372 at 2439.683 kb on + strand, within Synpcc7942_2372 at 2439.691 kb on - strand, within Synpcc7942_2372 at 2439.691 kb on - strand, within Synpcc7942_2372 at 2439.691 kb on - strand, within Synpcc7942_2372 at 2439.695 kb on + strand, within Synpcc7942_2372 at 2439.766 kb on - strand at 2439.770 kb on + strand, within Synpcc7942_2372 at 2439.840 kb on + strand, within Synpcc7942_2372 at 2439.848 kb on - strand, within Synpcc7942_2372 at 2439.857 kb on + strand, within Synpcc7942_2372 at 2439.857 kb on + strand, within Synpcc7942_2372 at 2439.857 kb on + strand, within Synpcc7942_2372 at 2439.865 kb on - strand, within Synpcc7942_2372 at 2439.918 kb on + strand, within Synpcc7942_2372 at 2439.928 kb on + strand, within Synpcc7942_2372 at 2439.940 kb on - strand, within Synpcc7942_2372 at 2439.959 kb on - strand, within Synpcc7942_2372 at 2439.971 kb on + strand, within Synpcc7942_2372 at 2439.997 kb on + strand, within Synpcc7942_2372 at 2439.997 kb on + strand, within Synpcc7942_2372 at 2440.005 kb on - strand, within Synpcc7942_2372 at 2440.034 kb on + strand, within Synpcc7942_2372 at 2440.034 kb on + strand, within Synpcc7942_2372 at 2440.034 kb on + strand, within Synpcc7942_2372 at 2440.042 kb on - strand, within Synpcc7942_2372 at 2440.068 kb on + strand, within Synpcc7942_2372 at 2440.070 kb on - strand, within Synpcc7942_2372 at 2440.070 kb on - strand, within Synpcc7942_2372 at 2440.088 kb on + strand, within Synpcc7942_2372 at 2440.101 kb on - strand, within Synpcc7942_2372 at 2440.139 kb on - strand, within Synpcc7942_2372 at 2440.139 kb on - strand, within Synpcc7942_2372 at 2440.181 kb on + strand, within Synpcc7942_2372
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,437,487 - acrF Synpcc7942_2369 0.77 -0.9 2,437,499 - acrF Synpcc7942_2369 0.78 -0.8 2,437,499 - acrF Synpcc7942_2369 0.78 -0.8 2,437,564 - acrF Synpcc7942_2369 0.80 -0.2 2,437,564 - acrF Synpcc7942_2369 0.80 -1.2 2,437,624 + acrF Synpcc7942_2369 0.81 -3.0 2,437,672 + acrF Synpcc7942_2369 0.83 -1.6 2,437,681 - acrF Synpcc7942_2369 0.83 +1.7 2,437,796 + acrF Synpcc7942_2369 0.87 -1.5 2,437,826 + acrF Synpcc7942_2369 0.88 -2.5 2,437,834 - acrF Synpcc7942_2369 0.88 -0.8 2,437,841 + acrF Synpcc7942_2369 0.88 +0.1 2,437,917 - -3.4 2,437,923 + -0.8 2,437,923 + +0.5 2,437,925 - -1.6 2,437,926 - -1.2 2,437,967 + +1.0 2,438,023 + +0.7 2,438,103 - -0.9 2,438,139 + -0.1 2,438,178 + -0.2 2,438,254 + Synpcc7942_2370 0.10 -3.0 2,438,259 + Synpcc7942_2370 0.13 -1.6 2,438,316 + Synpcc7942_2370 0.40 -1.5 2,438,322 + Synpcc7942_2370 0.43 -1.7 2,438,348 + Synpcc7942_2370 0.55 -0.3 2,438,364 + Synpcc7942_2370 0.63 +1.9 2,438,377 - Synpcc7942_2370 0.69 +0.7 2,438,377 - Synpcc7942_2370 0.69 +0.2 2,438,381 + Synpcc7942_2370 0.71 +1.0 2,438,399 - Synpcc7942_2370 0.80 -3.2 2,438,400 - Synpcc7942_2370 0.80 +0.3 2,438,424 + +1.8 2,438,449 + +2.6 2,438,515 + -3.6 2,438,627 - lpxA Synpcc7942_2371 0.22 -3.0 2,438,696 + lpxA Synpcc7942_2371 0.31 -0.3 2,439,055 + lpxA Synpcc7942_2371 0.76 -2.7 2,439,160 + lpxA Synpcc7942_2371 0.89 -3.1 2,439,253 + -0.9 2,439,283 - -2.5 2,439,283 - -0.3 2,439,284 + -2.0 2,439,285 + +2.0 2,439,293 - -2.2 2,439,293 - +2.9 2,439,293 - -1.3 2,439,293 - -1.0 2,439,293 - +1.8 2,439,293 - -1.7 2,439,307 + +3.8 2,439,309 - -0.6 2,439,346 + +9.5 2,439,351 + +5.9 2,439,354 - +5.9 2,439,354 - +3.9 2,439,354 - +6.5 2,439,354 - +4.7 2,439,354 - +3.1 2,439,354 - +5.7 2,439,359 - +3.4 2,439,359 - +5.9 2,439,382 + +5.9 2,439,408 + Synpcc7942_2372 0.12 +4.3 2,439,408 + Synpcc7942_2372 0.12 +5.6 2,439,416 - Synpcc7942_2372 0.12 +4.3 2,439,439 + Synpcc7942_2372 0.14 +2.9 2,439,444 + Synpcc7942_2372 0.15 +4.2 2,439,447 - Synpcc7942_2372 0.15 +4.3 2,439,452 - Synpcc7942_2372 0.16 +2.5 2,439,463 + Synpcc7942_2372 0.17 +3.5 2,439,463 - Synpcc7942_2372 0.17 +5.4 2,439,494 - Synpcc7942_2372 0.20 +5.5 2,439,499 - Synpcc7942_2372 0.20 +6.2 2,439,577 + Synpcc7942_2372 0.28 +3.9 2,439,599 + Synpcc7942_2372 0.30 +6.0 2,439,605 + Synpcc7942_2372 0.30 +4.3 2,439,632 + Synpcc7942_2372 0.33 +7.4 2,439,649 - Synpcc7942_2372 0.34 +6.6 2,439,659 - Synpcc7942_2372 0.35 +3.3 2,439,683 + Synpcc7942_2372 0.38 +5.8 2,439,691 - Synpcc7942_2372 0.38 +5.4 2,439,691 - Synpcc7942_2372 0.38 +3.1 2,439,691 - Synpcc7942_2372 0.38 +4.6 2,439,695 + Synpcc7942_2372 0.39 +4.3 2,439,766 - +4.2 2,439,770 + Synpcc7942_2372 0.46 +7.8 2,439,840 + Synpcc7942_2372 0.53 +6.0 2,439,848 - Synpcc7942_2372 0.53 +5.8 2,439,857 + Synpcc7942_2372 0.54 +5.4 2,439,857 + Synpcc7942_2372 0.54 +3.2 2,439,857 + Synpcc7942_2372 0.54 +3.5 2,439,865 - Synpcc7942_2372 0.55 +3.8 2,439,918 + Synpcc7942_2372 0.60 +4.1 2,439,928 + Synpcc7942_2372 0.61 +6.7 2,439,940 - Synpcc7942_2372 0.62 +5.9 2,439,959 - Synpcc7942_2372 0.64 +5.5 2,439,971 + Synpcc7942_2372 0.65 +5.7 2,439,997 + Synpcc7942_2372 0.68 +4.4 2,439,997 + Synpcc7942_2372 0.68 +6.9 2,440,005 - Synpcc7942_2372 0.68 +7.9 2,440,034 + Synpcc7942_2372 0.71 +9.1 2,440,034 + Synpcc7942_2372 0.71 +5.5 2,440,034 + Synpcc7942_2372 0.71 +5.9 2,440,042 - Synpcc7942_2372 0.72 +3.2 2,440,068 + Synpcc7942_2372 0.74 +5.8 2,440,070 - Synpcc7942_2372 0.75 +3.4 2,440,070 - Synpcc7942_2372 0.75 +9.3 2,440,088 + Synpcc7942_2372 0.76 +9.2 2,440,101 - Synpcc7942_2372 0.78 +6.2 2,440,139 - Synpcc7942_2372 0.81 +5.2 2,440,139 - Synpcc7942_2372 0.81 +0.3 2,440,181 + Synpcc7942_2372 0.85 +5.6
Or see this region's nucleotide sequence