Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fbp and Synpcc7942_2336 are separated by 260 nucleotides Synpcc7942_2336 and Synpcc7942_2337 are separated by 207 nucleotides Synpcc7942_2337 and Synpcc7942_2338 are separated by 298 nucleotides
Synpcc7942_2335: fbp - fructose-1,6-bisphosphatase, at 2,402,921 to 2,403,955
fbp
Synpcc7942_2336: Synpcc7942_2336 - hypothetical protein, at 2,404,216 to 2,404,389
_2336
Synpcc7942_2337: Synpcc7942_2337 - N-acetylmuramoyl-L-alanine amidase, family 2, at 2,404,597 to 2,405,550
_2337
Synpcc7942_2338: Synpcc7942_2338 - hypothetical protein, at 2,405,849 to 2,406,766
_2338
Position (kb)
2404
2405
2406 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6 at 2403.614 kb on + strand, within fbp at 2403.615 kb on + strand, within fbp at 2403.623 kb on - strand, within fbp at 2403.676 kb on - strand, within fbp at 2403.696 kb on + strand, within fbp at 2403.725 kb on + strand, within fbp at 2403.725 kb on + strand, within fbp at 2403.733 kb on - strand, within fbp at 2403.745 kb on - strand, within fbp at 2403.803 kb on - strand, within fbp at 2403.814 kb on + strand, within fbp at 2403.822 kb on - strand, within fbp at 2403.822 kb on - strand, within fbp at 2403.835 kb on - strand, within fbp at 2403.842 kb on + strand, within fbp at 2403.842 kb on + strand, within fbp at 2403.842 kb on + strand, within fbp at 2403.842 kb on + strand, within fbp at 2403.842 kb on + strand, within fbp at 2403.850 kb on - strand, within fbp at 2403.850 kb on - strand, within fbp at 2403.850 kb on - strand, within fbp at 2403.850 kb on - strand, within fbp at 2403.850 kb on - strand, within fbp at 2403.850 kb on - strand, within fbp at 2403.850 kb on - strand, within fbp at 2403.850 kb on - strand at 2403.856 kb on + strand at 2403.863 kb on + strand at 2403.883 kb on - strand at 2403.895 kb on + strand at 2403.908 kb on - strand at 2403.918 kb on + strand at 2403.926 kb on - strand at 2403.926 kb on - strand at 2403.944 kb on + strand at 2403.944 kb on + strand at 2403.947 kb on - strand at 2403.952 kb on - strand at 2403.967 kb on + strand at 2404.036 kb on + strand at 2404.040 kb on - strand at 2404.044 kb on + strand at 2404.053 kb on + strand at 2404.061 kb on - strand at 2404.068 kb on + strand at 2404.076 kb on - strand at 2404.076 kb on - strand at 2404.082 kb on + strand at 2404.175 kb on + strand at 2404.176 kb on + strand at 2404.177 kb on + strand at 2404.177 kb on + strand at 2404.177 kb on + strand at 2404.177 kb on + strand at 2404.185 kb on - strand at 2404.185 kb on - strand at 2404.185 kb on - strand at 2404.196 kb on - strand at 2404.214 kb on + strand at 2404.219 kb on + strand at 2404.222 kb on - strand at 2404.224 kb on + strand at 2404.224 kb on + strand at 2404.224 kb on + strand at 2404.227 kb on - strand at 2404.227 kb on - strand at 2404.227 kb on - strand at 2404.255 kb on - strand, within Synpcc7942_2336 at 2404.263 kb on + strand, within Synpcc7942_2336 at 2404.329 kb on + strand, within Synpcc7942_2336 at 2404.329 kb on + strand, within Synpcc7942_2336 at 2404.385 kb on + strand at 2404.388 kb on + strand at 2404.395 kb on - strand at 2404.411 kb on + strand at 2404.419 kb on - strand at 2404.419 kb on - strand at 2404.419 kb on - strand at 2404.419 kb on - strand at 2404.419 kb on - strand at 2404.419 kb on - strand at 2404.437 kb on - strand at 2404.460 kb on - strand at 2404.515 kb on + strand at 2404.530 kb on + strand at 2404.558 kb on + strand at 2404.566 kb on - strand at 2404.571 kb on + strand at 2404.579 kb on - strand at 2404.580 kb on - strand at 2404.603 kb on - strand at 2404.623 kb on - strand at 2404.625 kb on - strand at 2404.628 kb on - strand at 2404.648 kb on + strand at 2404.648 kb on + strand at 2404.648 kb on + strand at 2404.656 kb on - strand at 2404.674 kb on - strand at 2404.741 kb on - strand, within Synpcc7942_2337 at 2404.741 kb on - strand, within Synpcc7942_2337 at 2404.745 kb on + strand, within Synpcc7942_2337 at 2404.776 kb on + strand, within Synpcc7942_2337 at 2404.778 kb on + strand, within Synpcc7942_2337 at 2404.817 kb on - strand, within Synpcc7942_2337 at 2404.899 kb on - strand, within Synpcc7942_2337 at 2404.954 kb on - strand, within Synpcc7942_2337 at 2404.985 kb on + strand, within Synpcc7942_2337 at 2404.988 kb on - strand, within Synpcc7942_2337 at 2404.999 kb on - strand, within Synpcc7942_2337 at 2405.034 kb on + strand, within Synpcc7942_2337 at 2405.042 kb on - strand, within Synpcc7942_2337 at 2405.047 kb on + strand, within Synpcc7942_2337 at 2405.048 kb on + strand, within Synpcc7942_2337 at 2405.050 kb on - strand, within Synpcc7942_2337 at 2405.075 kb on - strand, within Synpcc7942_2337 at 2405.086 kb on + strand, within Synpcc7942_2337 at 2405.094 kb on - strand, within Synpcc7942_2337 at 2405.094 kb on - strand, within Synpcc7942_2337 at 2405.114 kb on + strand, within Synpcc7942_2337 at 2405.122 kb on - strand, within Synpcc7942_2337 at 2405.154 kb on - strand, within Synpcc7942_2337 at 2405.225 kb on + strand, within Synpcc7942_2337 at 2405.242 kb on + strand, within Synpcc7942_2337 at 2405.348 kb on + strand, within Synpcc7942_2337 at 2405.356 kb on - strand, within Synpcc7942_2337 at 2405.425 kb on - strand, within Synpcc7942_2337 at 2405.437 kb on + strand, within Synpcc7942_2337 at 2405.437 kb on + strand, within Synpcc7942_2337 at 2405.437 kb on + strand, within Synpcc7942_2337 at 2405.445 kb on - strand, within Synpcc7942_2337 at 2405.445 kb on - strand, within Synpcc7942_2337 at 2405.445 kb on - strand, within Synpcc7942_2337 at 2405.455 kb on - strand at 2405.464 kb on - strand at 2405.491 kb on - strand at 2405.493 kb on + strand at 2405.516 kb on + strand at 2405.524 kb on - strand at 2405.546 kb on + strand at 2405.596 kb on + strand at 2405.624 kb on + strand at 2405.669 kb on + strand at 2405.682 kb on + strand at 2405.694 kb on - strand at 2405.699 kb on - strand at 2405.715 kb on + strand at 2405.759 kb on - strand at 2405.764 kb on - strand at 2405.777 kb on - strand at 2405.791 kb on - strand at 2405.799 kb on + strand at 2405.812 kb on - strand at 2405.817 kb on - strand at 2405.845 kb on + strand at 2405.845 kb on + strand at 2405.853 kb on + strand at 2405.853 kb on + strand at 2405.853 kb on - strand at 2405.893 kb on - strand at 2405.898 kb on - strand at 2405.966 kb on + strand, within Synpcc7942_2338 at 2405.970 kb on + strand, within Synpcc7942_2338 at 2405.980 kb on + strand, within Synpcc7942_2338 at 2405.982 kb on + strand, within Synpcc7942_2338 at 2405.982 kb on + strand, within Synpcc7942_2338 at 2405.982 kb on + strand, within Synpcc7942_2338 at 2405.990 kb on - strand, within Synpcc7942_2338 at 2405.990 kb on - strand, within Synpcc7942_2338 at 2405.990 kb on - strand, within Synpcc7942_2338 at 2405.991 kb on + strand, within Synpcc7942_2338 at 2405.992 kb on + strand, within Synpcc7942_2338 at 2405.992 kb on + strand, within Synpcc7942_2338 at 2406.013 kb on + strand, within Synpcc7942_2338 at 2406.013 kb on + strand, within Synpcc7942_2338 at 2406.021 kb on - strand, within Synpcc7942_2338 at 2406.021 kb on - strand, within Synpcc7942_2338 at 2406.021 kb on - strand, within Synpcc7942_2338 at 2406.021 kb on - strand, within Synpcc7942_2338 at 2406.021 kb on - strand, within Synpcc7942_2338 at 2406.029 kb on + strand, within Synpcc7942_2338 at 2406.067 kb on + strand, within Synpcc7942_2338 at 2406.127 kb on + strand, within Synpcc7942_2338 at 2406.148 kb on + strand, within Synpcc7942_2338 at 2406.165 kb on + strand, within Synpcc7942_2338 at 2406.194 kb on + strand, within Synpcc7942_2338 at 2406.200 kb on - strand, within Synpcc7942_2338 at 2406.202 kb on - strand, within Synpcc7942_2338 at 2406.205 kb on + strand, within Synpcc7942_2338 at 2406.253 kb on + strand, within Synpcc7942_2338 at 2406.290 kb on + strand, within Synpcc7942_2338 at 2406.296 kb on - strand, within Synpcc7942_2338 at 2406.344 kb on + strand, within Synpcc7942_2338 at 2406.436 kb on - strand, within Synpcc7942_2338 at 2406.495 kb on + strand, within Synpcc7942_2338 at 2406.503 kb on - strand, within Synpcc7942_2338 at 2406.511 kb on + strand, within Synpcc7942_2338 at 2406.513 kb on - strand, within Synpcc7942_2338 at 2406.519 kb on - strand, within Synpcc7942_2338 at 2406.519 kb on - strand, within Synpcc7942_2338 at 2406.536 kb on - strand, within Synpcc7942_2338
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,403,614 + fbp Synpcc7942_2335 0.67 -0.6 2,403,615 + fbp Synpcc7942_2335 0.67 -2.7 2,403,623 - fbp Synpcc7942_2335 0.68 -3.5 2,403,676 - fbp Synpcc7942_2335 0.73 -0.6 2,403,696 + fbp Synpcc7942_2335 0.75 +1.6 2,403,725 + fbp Synpcc7942_2335 0.78 +1.8 2,403,725 + fbp Synpcc7942_2335 0.78 +2.2 2,403,733 - fbp Synpcc7942_2335 0.78 +0.2 2,403,745 - fbp Synpcc7942_2335 0.80 -0.4 2,403,803 - fbp Synpcc7942_2335 0.85 +0.2 2,403,814 + fbp Synpcc7942_2335 0.86 +6.5 2,403,822 - fbp Synpcc7942_2335 0.87 +0.7 2,403,822 - fbp Synpcc7942_2335 0.87 -0.0 2,403,835 - fbp Synpcc7942_2335 0.88 -1.6 2,403,842 + fbp Synpcc7942_2335 0.89 -1.5 2,403,842 + fbp Synpcc7942_2335 0.89 -2.9 2,403,842 + fbp Synpcc7942_2335 0.89 +0.6 2,403,842 + fbp Synpcc7942_2335 0.89 +3.3 2,403,842 + fbp Synpcc7942_2335 0.89 -0.4 2,403,850 - fbp Synpcc7942_2335 0.90 -1.4 2,403,850 - fbp Synpcc7942_2335 0.90 +0.2 2,403,850 - fbp Synpcc7942_2335 0.90 -0.9 2,403,850 - fbp Synpcc7942_2335 0.90 -2.3 2,403,850 - fbp Synpcc7942_2335 0.90 +0.1 2,403,850 - fbp Synpcc7942_2335 0.90 +1.7 2,403,850 - fbp Synpcc7942_2335 0.90 -1.9 2,403,850 - +2.0 2,403,856 + +2.7 2,403,863 + -0.4 2,403,883 - -0.8 2,403,895 + -1.3 2,403,908 - -1.1 2,403,918 + -0.0 2,403,926 - +1.9 2,403,926 - +0.4 2,403,944 + -1.6 2,403,944 + -1.3 2,403,947 - -1.3 2,403,952 - -0.8 2,403,967 + -2.2 2,404,036 + -0.1 2,404,040 - -0.3 2,404,044 + +2.9 2,404,053 + -1.2 2,404,061 - -1.0 2,404,068 + +1.4 2,404,076 - -0.9 2,404,076 - -0.3 2,404,082 + +0.5 2,404,175 + -2.3 2,404,176 + -0.8 2,404,177 + -2.1 2,404,177 + +0.7 2,404,177 + +1.4 2,404,177 + +0.2 2,404,185 - +1.5 2,404,185 - +0.4 2,404,185 - -3.2 2,404,196 - -0.1 2,404,214 + +0.4 2,404,219 + +0.5 2,404,222 - +2.1 2,404,224 + -1.6 2,404,224 + +2.3 2,404,224 + -0.4 2,404,227 - +1.1 2,404,227 - -1.7 2,404,227 - +0.2 2,404,255 - Synpcc7942_2336 0.22 +2.5 2,404,263 + Synpcc7942_2336 0.27 -0.5 2,404,329 + Synpcc7942_2336 0.65 +1.7 2,404,329 + Synpcc7942_2336 0.65 -0.8 2,404,385 + +0.6 2,404,388 + +0.9 2,404,395 - +1.7 2,404,411 + +1.5 2,404,419 - -0.0 2,404,419 - +0.4 2,404,419 - +0.2 2,404,419 - -2.3 2,404,419 - -0.1 2,404,419 - +0.3 2,404,437 - -1.4 2,404,460 - +0.8 2,404,515 + -0.5 2,404,530 + -2.5 2,404,558 + -1.8 2,404,566 - +1.5 2,404,571 + -0.7 2,404,579 - -1.6 2,404,580 - -0.3 2,404,603 - +0.8 2,404,623 - +1.0 2,404,625 - +2.1 2,404,628 - -2.4 2,404,648 + -0.3 2,404,648 + -2.4 2,404,648 + -1.9 2,404,656 - +0.5 2,404,674 - +2.8 2,404,741 - Synpcc7942_2337 0.15 -3.2 2,404,741 - Synpcc7942_2337 0.15 +1.8 2,404,745 + Synpcc7942_2337 0.16 -1.9 2,404,776 + Synpcc7942_2337 0.19 +2.8 2,404,778 + Synpcc7942_2337 0.19 -1.5 2,404,817 - Synpcc7942_2337 0.23 -1.1 2,404,899 - Synpcc7942_2337 0.32 +2.0 2,404,954 - Synpcc7942_2337 0.37 +5.0 2,404,985 + Synpcc7942_2337 0.41 +2.2 2,404,988 - Synpcc7942_2337 0.41 -1.0 2,404,999 - Synpcc7942_2337 0.42 -0.8 2,405,034 + Synpcc7942_2337 0.46 +0.3 2,405,042 - Synpcc7942_2337 0.47 +0.8 2,405,047 + Synpcc7942_2337 0.47 -2.3 2,405,048 + Synpcc7942_2337 0.47 +2.4 2,405,050 - Synpcc7942_2337 0.47 -1.5 2,405,075 - Synpcc7942_2337 0.50 -1.6 2,405,086 + Synpcc7942_2337 0.51 +4.2 2,405,094 - Synpcc7942_2337 0.52 +2.3 2,405,094 - Synpcc7942_2337 0.52 +1.1 2,405,114 + Synpcc7942_2337 0.54 -1.2 2,405,122 - Synpcc7942_2337 0.55 +0.9 2,405,154 - Synpcc7942_2337 0.58 +0.6 2,405,225 + Synpcc7942_2337 0.66 +3.4 2,405,242 + Synpcc7942_2337 0.68 -1.4 2,405,348 + Synpcc7942_2337 0.79 -0.9 2,405,356 - Synpcc7942_2337 0.80 -0.8 2,405,425 - Synpcc7942_2337 0.87 +0.7 2,405,437 + Synpcc7942_2337 0.88 +1.3 2,405,437 + Synpcc7942_2337 0.88 +1.5 2,405,437 + Synpcc7942_2337 0.88 +1.6 2,405,445 - Synpcc7942_2337 0.89 -2.1 2,405,445 - Synpcc7942_2337 0.89 +1.2 2,405,445 - Synpcc7942_2337 0.89 +2.6 2,405,455 - -2.1 2,405,464 - -1.0 2,405,491 - -0.3 2,405,493 + +0.8 2,405,516 + +1.6 2,405,524 - -2.3 2,405,546 + -3.2 2,405,596 + -0.3 2,405,624 + -0.3 2,405,669 + +1.5 2,405,682 + +2.0 2,405,694 - +1.8 2,405,699 - -2.6 2,405,715 + -0.0 2,405,759 - +1.8 2,405,764 - +2.1 2,405,777 - -2.2 2,405,791 - +1.9 2,405,799 + +0.9 2,405,812 - +2.9 2,405,817 - +3.2 2,405,845 + -0.9 2,405,845 + +1.3 2,405,853 + +0.4 2,405,853 + -1.0 2,405,853 - +1.3 2,405,893 - -1.7 2,405,898 - +3.4 2,405,966 + Synpcc7942_2338 0.13 -1.0 2,405,970 + Synpcc7942_2338 0.13 -5.1 2,405,980 + Synpcc7942_2338 0.14 -2.5 2,405,982 + Synpcc7942_2338 0.14 -3.5 2,405,982 + Synpcc7942_2338 0.14 -3.2 2,405,982 + Synpcc7942_2338 0.14 -3.2 2,405,990 - Synpcc7942_2338 0.15 -0.5 2,405,990 - Synpcc7942_2338 0.15 -0.4 2,405,990 - Synpcc7942_2338 0.15 -1.6 2,405,991 + Synpcc7942_2338 0.15 -2.3 2,405,992 + Synpcc7942_2338 0.16 -1.2 2,405,992 + Synpcc7942_2338 0.16 -0.2 2,406,013 + Synpcc7942_2338 0.18 -0.7 2,406,013 + Synpcc7942_2338 0.18 +0.7 2,406,021 - Synpcc7942_2338 0.19 +1.8 2,406,021 - Synpcc7942_2338 0.19 -0.4 2,406,021 - Synpcc7942_2338 0.19 +1.2 2,406,021 - Synpcc7942_2338 0.19 -1.2 2,406,021 - Synpcc7942_2338 0.19 -0.0 2,406,029 + Synpcc7942_2338 0.20 -1.0 2,406,067 + Synpcc7942_2338 0.24 -1.3 2,406,127 + Synpcc7942_2338 0.30 +0.6 2,406,148 + Synpcc7942_2338 0.33 -1.3 2,406,165 + Synpcc7942_2338 0.34 -0.6 2,406,194 + Synpcc7942_2338 0.38 -2.0 2,406,200 - Synpcc7942_2338 0.38 -1.6 2,406,202 - Synpcc7942_2338 0.38 -2.5 2,406,205 + Synpcc7942_2338 0.39 -0.3 2,406,253 + Synpcc7942_2338 0.44 -1.5 2,406,290 + Synpcc7942_2338 0.48 +0.4 2,406,296 - Synpcc7942_2338 0.49 +0.2 2,406,344 + Synpcc7942_2338 0.54 -0.7 2,406,436 - Synpcc7942_2338 0.64 -1.3 2,406,495 + Synpcc7942_2338 0.70 -2.6 2,406,503 - Synpcc7942_2338 0.71 -2.9 2,406,511 + Synpcc7942_2338 0.72 -0.1 2,406,513 - Synpcc7942_2338 0.72 +1.7 2,406,519 - Synpcc7942_2338 0.73 -2.6 2,406,519 - Synpcc7942_2338 0.73 -1.9 2,406,536 - Synpcc7942_2338 0.75 -0.9
Or see this region's nucleotide sequence