Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppnK and nblR are separated by 44 nucleotides nblR and Synpcc7942_2306 are separated by 132 nucleotides Synpcc7942_2306 and Synpcc7942_2307 are separated by 3 nucleotides
Synpcc7942_2304: ppnK - inorganic polyphosphate/ATP-NAD kinase, at 2,372,353 to 2,373,270
ppnK
Synpcc7942_2305: nblR - two component transcriptional regulator, winged helix family, at 2,373,315 to 2,374,001
nblR
Synpcc7942_2306: Synpcc7942_2306 - Heat shock protein DnaJ-like, at 2,374,134 to 2,374,721
_2306
Synpcc7942_2307: Synpcc7942_2307 - hypothetical protein, at 2,374,725 to 2,375,735
_2307
Position (kb)
2374
2375 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2373.153 kb on + strand, within ppnK at 2373.246 kb on + strand at 2373.302 kb on - strand at 2373.317 kb on + strand at 2373.560 kb on + strand, within nblR at 2373.626 kb on - strand, within nblR at 2373.869 kb on - strand, within nblR at 2373.973 kb on + strand at 2374.025 kb on + strand at 2374.038 kb on - strand at 2374.084 kb on + strand at 2374.084 kb on + strand at 2374.092 kb on - strand at 2374.105 kb on + strand at 2374.114 kb on - strand at 2374.260 kb on + strand, within Synpcc7942_2306 at 2374.283 kb on + strand, within Synpcc7942_2306 at 2374.293 kb on + strand, within Synpcc7942_2306 at 2374.301 kb on + strand, within Synpcc7942_2306 at 2374.331 kb on - strand at 2374.331 kb on - strand, within Synpcc7942_2306 at 2374.338 kb on - strand, within Synpcc7942_2306 at 2374.338 kb on - strand, within Synpcc7942_2306 at 2374.906 kb on + strand, within Synpcc7942_2307 at 2374.935 kb on - strand, within Synpcc7942_2307 at 2375.437 kb on + strand, within Synpcc7942_2307 at 2375.505 kb on - strand, within Synpcc7942_2307 at 2375.586 kb on - strand, within Synpcc7942_2307 at 2375.603 kb on + strand, within Synpcc7942_2307 at 2375.611 kb on - strand, within Synpcc7942_2307 at 2375.715 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,373,153 + ppnK Synpcc7942_2304 0.87 -0.6 2,373,246 + -1.2 2,373,302 - +1.5 2,373,317 + +0.9 2,373,560 + nblR Synpcc7942_2305 0.36 -1.5 2,373,626 - nblR Synpcc7942_2305 0.45 -3.4 2,373,869 - nblR Synpcc7942_2305 0.81 -3.9 2,373,973 + -1.6 2,374,025 + +1.2 2,374,038 - -0.3 2,374,084 + -0.6 2,374,084 + +0.4 2,374,092 - -0.2 2,374,105 + -3.0 2,374,114 - -1.7 2,374,260 + Synpcc7942_2306 0.21 -2.9 2,374,283 + Synpcc7942_2306 0.25 -0.9 2,374,293 + Synpcc7942_2306 0.27 -3.7 2,374,301 + Synpcc7942_2306 0.28 -1.9 2,374,331 - -2.4 2,374,331 - Synpcc7942_2306 0.34 -6.4 2,374,338 - Synpcc7942_2306 0.35 -1.6 2,374,338 - Synpcc7942_2306 0.35 -5.2 2,374,906 + Synpcc7942_2307 0.18 +1.5 2,374,935 - Synpcc7942_2307 0.21 -0.6 2,375,437 + Synpcc7942_2307 0.70 -1.4 2,375,505 - Synpcc7942_2307 0.77 +1.2 2,375,586 - Synpcc7942_2307 0.85 +0.9 2,375,603 + Synpcc7942_2307 0.87 -1.9 2,375,611 - Synpcc7942_2307 0.88 -1.1 2,375,715 + +0.4
Or see this region's nucleotide sequence