Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt chlD and tRNA-Arg are separated by 585 nucleotides tRNA-Arg and Synpcc7942_2275 overlap by 27 nucleotides Synpcc7942_2275 and Synpcc7942_2276 overlap by 34 nucleotides Synpcc7942_2276 and Synpcc7942_2277 are separated by 256 nucleotides Synpcc7942_2277 and Synpcc7942_2278 are separated by 193 nucleotides
Synpcc7942_2274: chlD - protoporphyrin IX magnesium-chelatase, at 2,340,963 to 2,342,996
chlD
Synpcc7942_R0011: tRNA-Arg - tRNA-Arg, at 2,343,582 to 2,343,657
tRNA-Arg
Synpcc7942_2275: Synpcc7942_2275 - hypothetical protein, at 2,343,631 to 2,343,915
_2275
Synpcc7942_2276: Synpcc7942_2276 - hypothetical protein, at 2,343,882 to 2,344,334
_2276
Synpcc7942_2277: Synpcc7942_2277 - hypothetical protein, at 2,344,591 to 2,344,899
_2277
Synpcc7942_2278: Synpcc7942_2278 - hypothetical protein, at 2,345,093 to 2,347,240
_2278
Position (kb)
2343
2344
2345 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7 at 2343.019 kb on + strand at 2343.034 kb on + strand at 2343.212 kb on - strand at 2343.221 kb on - strand at 2343.406 kb on - strand at 2343.447 kb on + strand at 2343.447 kb on + strand at 2343.455 kb on - strand at 2343.499 kb on - strand at 2343.505 kb on - strand at 2343.524 kb on - strand at 2343.715 kb on + strand, within Synpcc7942_2275 at 2343.718 kb on - strand, within Synpcc7942_2275 at 2343.780 kb on - strand, within Synpcc7942_2275 at 2343.791 kb on + strand, within Synpcc7942_2275 at 2343.866 kb on + strand, within Synpcc7942_2275 at 2343.909 kb on + strand at 2343.909 kb on + strand at 2343.917 kb on - strand at 2343.917 kb on - strand at 2343.932 kb on - strand, within Synpcc7942_2276 at 2343.952 kb on - strand, within Synpcc7942_2276 at 2343.959 kb on - strand, within Synpcc7942_2276 at 2343.970 kb on + strand, within Synpcc7942_2276 at 2343.970 kb on + strand, within Synpcc7942_2276 at 2343.995 kb on + strand, within Synpcc7942_2276 at 2344.003 kb on - strand, within Synpcc7942_2276 at 2344.005 kb on + strand, within Synpcc7942_2276 at 2344.005 kb on + strand, within Synpcc7942_2276 at 2344.013 kb on - strand, within Synpcc7942_2276 at 2344.028 kb on - strand, within Synpcc7942_2276 at 2344.029 kb on - strand, within Synpcc7942_2276 at 2344.040 kb on + strand, within Synpcc7942_2276 at 2344.053 kb on + strand, within Synpcc7942_2276 at 2344.082 kb on - strand, within Synpcc7942_2276 at 2344.132 kb on + strand, within Synpcc7942_2276 at 2344.140 kb on - strand, within Synpcc7942_2276 at 2344.140 kb on - strand at 2344.142 kb on + strand, within Synpcc7942_2276 at 2344.176 kb on - strand, within Synpcc7942_2276 at 2344.247 kb on + strand, within Synpcc7942_2276 at 2344.249 kb on + strand, within Synpcc7942_2276 at 2344.376 kb on + strand at 2344.403 kb on - strand at 2344.404 kb on - strand at 2344.426 kb on + strand at 2344.451 kb on - strand at 2344.485 kb on - strand at 2344.486 kb on - strand at 2344.499 kb on + strand at 2344.587 kb on + strand at 2344.604 kb on + strand at 2344.619 kb on + strand at 2344.638 kb on + strand, within Synpcc7942_2277 at 2344.698 kb on - strand, within Synpcc7942_2277 at 2344.746 kb on - strand, within Synpcc7942_2277 at 2344.763 kb on + strand, within Synpcc7942_2277 at 2344.777 kb on + strand, within Synpcc7942_2277 at 2344.784 kb on - strand, within Synpcc7942_2277 at 2344.790 kb on - strand, within Synpcc7942_2277 at 2344.849 kb on - strand, within Synpcc7942_2277 at 2344.858 kb on + strand, within Synpcc7942_2277 at 2344.858 kb on + strand, within Synpcc7942_2277 at 2344.858 kb on + strand, within Synpcc7942_2277 at 2344.866 kb on - strand, within Synpcc7942_2277 at 2344.888 kb on - strand at 2344.913 kb on - strand at 2344.918 kb on - strand at 2344.918 kb on - strand at 2344.953 kb on + strand at 2344.953 kb on + strand at 2344.953 kb on + strand at 2344.963 kb on + strand at 2344.963 kb on + strand at 2344.974 kb on + strand at 2344.974 kb on + strand at 2345.054 kb on + strand at 2345.056 kb on + strand at 2345.070 kb on + strand at 2345.078 kb on - strand at 2345.090 kb on + strand at 2345.092 kb on - strand at 2345.098 kb on + strand at 2345.131 kb on - strand at 2345.181 kb on - strand at 2345.181 kb on - strand at 2345.181 kb on - strand at 2345.215 kb on - strand at 2345.239 kb on + strand at 2345.239 kb on + strand at 2345.242 kb on + strand at 2345.242 kb on + strand at 2345.247 kb on - strand at 2345.250 kb on - strand at 2345.266 kb on + strand at 2345.272 kb on + strand at 2345.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,343,019 + +3.2 2,343,034 + +0.5 2,343,212 - -1.1 2,343,221 - -0.1 2,343,406 - +2.9 2,343,447 + -1.3 2,343,447 + -1.8 2,343,455 - +1.7 2,343,499 - -0.1 2,343,505 - +1.2 2,343,524 - -1.3 2,343,715 + Synpcc7942_2275 0.29 -1.6 2,343,718 - Synpcc7942_2275 0.31 +0.4 2,343,780 - Synpcc7942_2275 0.52 -2.1 2,343,791 + Synpcc7942_2275 0.56 -1.2 2,343,866 + Synpcc7942_2275 0.82 +2.4 2,343,909 + +7.5 2,343,909 + -1.1 2,343,917 - +0.9 2,343,917 - -1.2 2,343,932 - Synpcc7942_2276 0.11 +3.8 2,343,952 - Synpcc7942_2276 0.15 -2.4 2,343,959 - Synpcc7942_2276 0.17 +2.7 2,343,970 + Synpcc7942_2276 0.19 -1.4 2,343,970 + Synpcc7942_2276 0.19 -0.1 2,343,995 + Synpcc7942_2276 0.25 +3.7 2,344,003 - Synpcc7942_2276 0.27 +3.8 2,344,005 + Synpcc7942_2276 0.27 +3.2 2,344,005 + Synpcc7942_2276 0.27 +1.2 2,344,013 - Synpcc7942_2276 0.29 -0.6 2,344,028 - Synpcc7942_2276 0.32 +2.8 2,344,029 - Synpcc7942_2276 0.32 +2.5 2,344,040 + Synpcc7942_2276 0.35 -1.4 2,344,053 + Synpcc7942_2276 0.38 +1.8 2,344,082 - Synpcc7942_2276 0.44 +3.4 2,344,132 + Synpcc7942_2276 0.55 +3.6 2,344,140 - Synpcc7942_2276 0.57 +1.4 2,344,140 - +3.8 2,344,142 + Synpcc7942_2276 0.57 +3.8 2,344,176 - Synpcc7942_2276 0.65 +4.0 2,344,247 + Synpcc7942_2276 0.81 +1.6 2,344,249 + Synpcc7942_2276 0.81 +3.3 2,344,376 + -1.1 2,344,403 - -2.4 2,344,404 - -2.2 2,344,426 + +0.3 2,344,451 - -1.4 2,344,485 - +0.2 2,344,486 - -1.4 2,344,499 + -2.0 2,344,587 + +0.1 2,344,604 + -1.9 2,344,619 + -1.7 2,344,638 + Synpcc7942_2277 0.15 +1.9 2,344,698 - Synpcc7942_2277 0.35 +2.3 2,344,746 - Synpcc7942_2277 0.50 +0.8 2,344,763 + Synpcc7942_2277 0.56 -2.1 2,344,777 + Synpcc7942_2277 0.60 +3.8 2,344,784 - Synpcc7942_2277 0.62 +0.4 2,344,790 - Synpcc7942_2277 0.64 +1.0 2,344,849 - Synpcc7942_2277 0.83 +1.0 2,344,858 + Synpcc7942_2277 0.86 -0.7 2,344,858 + Synpcc7942_2277 0.86 -2.7 2,344,858 + Synpcc7942_2277 0.86 +3.2 2,344,866 - Synpcc7942_2277 0.89 -1.0 2,344,888 - -2.4 2,344,913 - +1.5 2,344,918 - +1.6 2,344,918 - +3.7 2,344,953 + -2.5 2,344,953 + -1.0 2,344,953 + +0.0 2,344,963 + +1.1 2,344,963 + -2.1 2,344,974 + -2.1 2,344,974 + -1.4 2,345,054 + +0.4 2,345,056 + -0.6 2,345,070 + +2.5 2,345,078 - -0.8 2,345,090 + -2.7 2,345,092 - -2.1 2,345,098 + -3.2 2,345,131 - +1.7 2,345,181 - -0.9 2,345,181 - -1.0 2,345,181 - +3.7 2,345,215 - -1.3 2,345,239 + +0.3 2,345,239 + +0.6 2,345,242 + -0.3 2,345,242 + +2.1 2,345,247 - +1.9 2,345,250 - +0.3 2,345,266 + +5.7 2,345,272 + +2.1 2,345,280 - -0.0
Or see this region's nucleotide sequence