Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaE and Synpcc7942_2200 are separated by 57 nucleotides Synpcc7942_2200 and tRNA-Ser are separated by 122 nucleotides tRNA-Ser and alr are separated by 38 nucleotides alr and mcrA are separated by 124 nucleotides mcrA and prfA are separated by 13 nucleotides
Synpcc7942_2199: dnaE - DNA polymerase III subunit alpha, at 2,277,916 to 2,280,528
dnaE
Synpcc7942_2200: Synpcc7942_2200 - hypothetical protein, at 2,280,586 to 2,281,077
_2200
Synpcc7942_R0008: tRNA-Ser - tRNA-Ser, at 2,281,200 to 2,281,288
tRNA-Ser
Synpcc7942_2201: alr - alanine racemase, at 2,281,327 to 2,282,547
alr
Synpcc7942_2202: mcrA - HNH nuclease, at 2,282,672 to 2,283,169
mcrA
Synpcc7942_2203: prfA - peptide chain release factor 1, at 2,283,183 to 2,284,289
prfA
Position (kb)
2281
2282
2283 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2280.548 kb on - strand at 2280.553 kb on + strand at 2280.583 kb on - strand at 2280.588 kb on - strand at 2280.597 kb on - strand at 2280.635 kb on + strand at 2280.635 kb on + strand at 2280.692 kb on + strand, within Synpcc7942_2200 at 2280.713 kb on - strand, within Synpcc7942_2200 at 2280.715 kb on - strand, within Synpcc7942_2200 at 2280.715 kb on - strand, within Synpcc7942_2200 at 2280.725 kb on - strand, within Synpcc7942_2200 at 2280.725 kb on - strand, within Synpcc7942_2200 at 2280.747 kb on + strand, within Synpcc7942_2200 at 2280.749 kb on + strand, within Synpcc7942_2200 at 2280.757 kb on - strand, within Synpcc7942_2200 at 2280.767 kb on - strand, within Synpcc7942_2200 at 2280.837 kb on - strand, within Synpcc7942_2200 at 2280.841 kb on + strand, within Synpcc7942_2200 at 2280.844 kb on - strand, within Synpcc7942_2200 at 2280.893 kb on + strand, within Synpcc7942_2200 at 2280.909 kb on + strand, within Synpcc7942_2200 at 2280.917 kb on - strand, within Synpcc7942_2200 at 2280.917 kb on - strand, within Synpcc7942_2200 at 2280.917 kb on - strand, within Synpcc7942_2200 at 2280.952 kb on + strand, within Synpcc7942_2200 at 2280.960 kb on - strand, within Synpcc7942_2200 at 2280.964 kb on - strand, within Synpcc7942_2200 at 2280.964 kb on - strand, within Synpcc7942_2200 at 2280.966 kb on + strand, within Synpcc7942_2200 at 2280.971 kb on + strand, within Synpcc7942_2200 at 2280.974 kb on - strand, within Synpcc7942_2200 at 2280.976 kb on + strand, within Synpcc7942_2200 at 2280.979 kb on - strand, within Synpcc7942_2200 at 2281.054 kb on - strand at 2281.082 kb on + strand at 2281.088 kb on + strand at 2281.090 kb on - strand at 2281.162 kb on + strand at 2281.326 kb on + strand at 2281.334 kb on - strand at 2282.504 kb on + strand at 2282.535 kb on + strand at 2282.557 kb on + strand at 2282.557 kb on + strand at 2282.557 kb on + strand at 2282.565 kb on - strand at 2282.587 kb on - strand at 2282.599 kb on - strand at 2282.608 kb on - strand at 2282.628 kb on + strand at 2282.681 kb on + strand at 2282.681 kb on - strand at 2282.708 kb on + strand at 2282.769 kb on + strand, within mcrA at 2282.777 kb on - strand, within mcrA at 2282.839 kb on - strand, within mcrA at 2282.864 kb on + strand, within mcrA at 2282.864 kb on + strand at 2282.865 kb on + strand, within mcrA at 2282.911 kb on + strand, within mcrA at 2283.059 kb on + strand, within mcrA at 2283.059 kb on + strand, within mcrA at 2283.064 kb on + strand, within mcrA at 2283.064 kb on + strand, within mcrA at 2283.067 kb on - strand at 2283.072 kb on - strand, within mcrA at 2283.072 kb on - strand, within mcrA at 2283.084 kb on - strand, within mcrA at 2283.117 kb on + strand, within mcrA at 2283.125 kb on - strand at 2283.181 kb on + strand at 2283.184 kb on - strand at 2283.189 kb on - strand at 2283.198 kb on + strand at 2283.208 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,280,548 - -3.8 2,280,553 + -2.3 2,280,583 - -1.6 2,280,588 - +0.7 2,280,597 - +3.2 2,280,635 + +2.2 2,280,635 + -0.6 2,280,692 + Synpcc7942_2200 0.22 -0.9 2,280,713 - Synpcc7942_2200 0.26 -1.3 2,280,715 - Synpcc7942_2200 0.26 +3.0 2,280,715 - Synpcc7942_2200 0.26 -0.4 2,280,725 - Synpcc7942_2200 0.28 +1.0 2,280,725 - Synpcc7942_2200 0.28 -0.7 2,280,747 + Synpcc7942_2200 0.33 -1.0 2,280,749 + Synpcc7942_2200 0.33 +3.3 2,280,757 - Synpcc7942_2200 0.35 -2.2 2,280,767 - Synpcc7942_2200 0.37 -1.2 2,280,837 - Synpcc7942_2200 0.51 -0.9 2,280,841 + Synpcc7942_2200 0.52 -2.4 2,280,844 - Synpcc7942_2200 0.52 -1.6 2,280,893 + Synpcc7942_2200 0.62 +1.2 2,280,909 + Synpcc7942_2200 0.66 -0.8 2,280,917 - Synpcc7942_2200 0.67 -1.6 2,280,917 - Synpcc7942_2200 0.67 +2.0 2,280,917 - Synpcc7942_2200 0.67 -1.8 2,280,952 + Synpcc7942_2200 0.74 +2.1 2,280,960 - Synpcc7942_2200 0.76 -2.0 2,280,964 - Synpcc7942_2200 0.77 -1.3 2,280,964 - Synpcc7942_2200 0.77 -1.1 2,280,966 + Synpcc7942_2200 0.77 -0.5 2,280,971 + Synpcc7942_2200 0.78 +1.6 2,280,974 - Synpcc7942_2200 0.79 +0.5 2,280,976 + Synpcc7942_2200 0.79 -0.4 2,280,979 - Synpcc7942_2200 0.80 -0.8 2,281,054 - +3.2 2,281,082 + +1.7 2,281,088 + -2.0 2,281,090 - +0.6 2,281,162 + -3.9 2,281,326 + -0.8 2,281,334 - +2.8 2,282,504 + -4.2 2,282,535 + +0.8 2,282,557 + +0.4 2,282,557 + -2.2 2,282,557 + -3.5 2,282,565 - -3.4 2,282,587 - -3.4 2,282,599 - -1.9 2,282,608 - -1.7 2,282,628 + +1.8 2,282,681 + +3.5 2,282,681 - -0.5 2,282,708 + +1.4 2,282,769 + mcrA Synpcc7942_2202 0.19 +1.5 2,282,777 - mcrA Synpcc7942_2202 0.21 +1.6 2,282,839 - mcrA Synpcc7942_2202 0.34 +3.6 2,282,864 + mcrA Synpcc7942_2202 0.39 -0.4 2,282,864 + -0.0 2,282,865 + mcrA Synpcc7942_2202 0.39 -1.3 2,282,911 + mcrA Synpcc7942_2202 0.48 -0.5 2,283,059 + mcrA Synpcc7942_2202 0.78 -2.6 2,283,059 + mcrA Synpcc7942_2202 0.78 +1.1 2,283,064 + mcrA Synpcc7942_2202 0.79 +1.3 2,283,064 + mcrA Synpcc7942_2202 0.79 -0.0 2,283,067 - +0.4 2,283,072 - mcrA Synpcc7942_2202 0.80 +0.3 2,283,072 - mcrA Synpcc7942_2202 0.80 -0.3 2,283,084 - mcrA Synpcc7942_2202 0.83 +1.8 2,283,117 + mcrA Synpcc7942_2202 0.89 -0.8 2,283,125 - +1.0 2,283,181 + -1.5 2,283,184 - +2.0 2,283,189 - +4.0 2,283,198 + +0.2 2,283,208 + -0.0
Or see this region's nucleotide sequence