Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menF and Synpcc7942_2189 are separated by 84 nucleotides Synpcc7942_2189 and msrB are separated by 52 nucleotides msrB and Synpcc7942_2191 are separated by 65 nucleotides
Synpcc7942_2188: menF - isochorismate synthase, at 2,266,131 to 2,267,534
menF
Synpcc7942_2189: Synpcc7942_2189 - hypothetical protein, at 2,267,619 to 2,267,882
_2189
Synpcc7942_2190: msrB - Methionine sulfoxide reductase B, at 2,267,935 to 2,268,357
msrB
Synpcc7942_2191: Synpcc7942_2191 - hypothetical protein, at 2,268,423 to 2,269,154
_2191
Position (kb)
2267
2268 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2267.494 kb on + strand at 2267.565 kb on + strand at 2267.565 kb on + strand at 2267.573 kb on - strand at 2267.573 kb on - strand at 2267.573 kb on - strand at 2267.573 kb on - strand at 2267.573 kb on - strand at 2267.573 kb on - strand at 2267.643 kb on + strand at 2267.646 kb on + strand, within Synpcc7942_2189 at 2267.654 kb on - strand, within Synpcc7942_2189 at 2267.666 kb on - strand, within Synpcc7942_2189 at 2267.666 kb on - strand, within Synpcc7942_2189 at 2267.692 kb on + strand, within Synpcc7942_2189 at 2267.706 kb on + strand, within Synpcc7942_2189 at 2267.714 kb on - strand, within Synpcc7942_2189 at 2267.714 kb on - strand, within Synpcc7942_2189 at 2267.724 kb on - strand, within Synpcc7942_2189 at 2267.743 kb on - strand, within Synpcc7942_2189 at 2267.745 kb on + strand, within Synpcc7942_2189 at 2267.745 kb on + strand, within Synpcc7942_2189 at 2267.745 kb on + strand, within Synpcc7942_2189 at 2267.745 kb on + strand, within Synpcc7942_2189 at 2267.745 kb on + strand, within Synpcc7942_2189 at 2267.753 kb on - strand, within Synpcc7942_2189 at 2267.763 kb on - strand, within Synpcc7942_2189 at 2267.765 kb on - strand, within Synpcc7942_2189 at 2267.786 kb on - strand, within Synpcc7942_2189 at 2267.866 kb on + strand at 2267.867 kb on - strand at 2267.874 kb on - strand at 2267.876 kb on - strand at 2267.916 kb on + strand at 2267.916 kb on - strand at 2267.922 kb on - strand at 2267.925 kb on + strand at 2267.933 kb on - strand at 2267.979 kb on + strand, within msrB at 2267.984 kb on + strand, within msrB at 2267.984 kb on + strand, within msrB at 2267.984 kb on + strand, within msrB at 2267.984 kb on + strand, within msrB at 2267.984 kb on + strand, within msrB at 2267.984 kb on + strand, within msrB at 2267.984 kb on + strand, within msrB at 2267.984 kb on + strand, within msrB at 2267.986 kb on + strand, within msrB at 2267.992 kb on - strand, within msrB at 2268.002 kb on - strand, within msrB at 2268.047 kb on - strand, within msrB at 2268.089 kb on + strand, within msrB at 2268.091 kb on - strand, within msrB at 2268.091 kb on - strand, within msrB at 2268.102 kb on - strand, within msrB at 2268.102 kb on - strand, within msrB at 2268.107 kb on - strand, within msrB at 2268.243 kb on + strand, within msrB at 2268.280 kb on + strand, within msrB at 2268.285 kb on + strand, within msrB at 2268.285 kb on + strand, within msrB at 2268.288 kb on - strand, within msrB at 2268.307 kb on - strand, within msrB at 2268.344 kb on + strand at 2268.352 kb on + strand at 2268.352 kb on + strand at 2268.352 kb on + strand at 2268.352 kb on + strand at 2268.360 kb on - strand at 2268.385 kb on + strand at 2268.397 kb on + strand at 2268.400 kb on + strand at 2268.405 kb on - strand at 2268.447 kb on + strand at 2268.461 kb on + strand at 2268.463 kb on + strand at 2268.469 kb on - strand at 2268.471 kb on - strand at 2268.471 kb on - strand at 2268.471 kb on - strand at 2268.479 kb on - strand at 2268.483 kb on + strand at 2268.483 kb on + strand at 2268.502 kb on - strand, within Synpcc7942_2191 at 2268.517 kb on + strand, within Synpcc7942_2191 at 2268.544 kb on + strand, within Synpcc7942_2191 at 2268.554 kb on + strand, within Synpcc7942_2191 at 2268.562 kb on + strand, within Synpcc7942_2191 at 2268.563 kb on - strand, within Synpcc7942_2191 at 2268.570 kb on - strand, within Synpcc7942_2191 at 2268.578 kb on - strand at 2268.590 kb on + strand, within Synpcc7942_2191 at 2268.590 kb on + strand, within Synpcc7942_2191 at 2268.598 kb on - strand, within Synpcc7942_2191 at 2268.598 kb on - strand, within Synpcc7942_2191 at 2268.598 kb on - strand, within Synpcc7942_2191 at 2268.598 kb on - strand, within Synpcc7942_2191 at 2268.598 kb on - strand, within Synpcc7942_2191 at 2268.598 kb on - strand, within Synpcc7942_2191 at 2268.608 kb on + strand, within Synpcc7942_2191 at 2268.608 kb on + strand, within Synpcc7942_2191 at 2268.613 kb on + strand, within Synpcc7942_2191 at 2268.613 kb on + strand, within Synpcc7942_2191 at 2268.613 kb on + strand, within Synpcc7942_2191 at 2268.616 kb on + strand, within Synpcc7942_2191 at 2268.616 kb on - strand, within Synpcc7942_2191 at 2268.616 kb on - strand, within Synpcc7942_2191 at 2268.618 kb on + strand, within Synpcc7942_2191 at 2268.618 kb on + strand, within Synpcc7942_2191 at 2268.621 kb on - strand, within Synpcc7942_2191 at 2268.621 kb on - strand, within Synpcc7942_2191 at 2268.621 kb on - strand, within Synpcc7942_2191 at 2268.621 kb on - strand, within Synpcc7942_2191 at 2268.626 kb on - strand, within Synpcc7942_2191 at 2268.626 kb on - strand, within Synpcc7942_2191 at 2268.626 kb on - strand, within Synpcc7942_2191 at 2268.630 kb on + strand, within Synpcc7942_2191 at 2268.633 kb on - strand, within Synpcc7942_2191 at 2268.652 kb on + strand, within Synpcc7942_2191 at 2268.660 kb on - strand, within Synpcc7942_2191 at 2268.662 kb on + strand, within Synpcc7942_2191 at 2268.662 kb on + strand, within Synpcc7942_2191 at 2268.686 kb on + strand, within Synpcc7942_2191 at 2268.691 kb on + strand, within Synpcc7942_2191 at 2268.718 kb on - strand, within Synpcc7942_2191 at 2268.750 kb on - strand, within Synpcc7942_2191 at 2268.755 kb on - strand, within Synpcc7942_2191 at 2268.776 kb on - strand, within Synpcc7942_2191 at 2268.778 kb on + strand, within Synpcc7942_2191 at 2268.809 kb on + strand, within Synpcc7942_2191 at 2268.809 kb on + strand, within Synpcc7942_2191 at 2268.809 kb on + strand, within Synpcc7942_2191 at 2268.817 kb on - strand, within Synpcc7942_2191 at 2268.827 kb on - strand, within Synpcc7942_2191 at 2268.852 kb on - strand, within Synpcc7942_2191 at 2268.870 kb on - strand, within Synpcc7942_2191 at 2268.876 kb on - strand, within Synpcc7942_2191
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,267,494 + -1.9 2,267,565 + -0.5 2,267,565 + -0.2 2,267,573 - -0.9 2,267,573 - -1.4 2,267,573 - +0.8 2,267,573 - -3.4 2,267,573 - -0.8 2,267,573 - -0.6 2,267,643 + -0.7 2,267,646 + Synpcc7942_2189 0.10 +1.9 2,267,654 - Synpcc7942_2189 0.13 -1.5 2,267,666 - Synpcc7942_2189 0.18 -1.6 2,267,666 - Synpcc7942_2189 0.18 +2.0 2,267,692 + Synpcc7942_2189 0.28 +1.4 2,267,706 + Synpcc7942_2189 0.33 +0.7 2,267,714 - Synpcc7942_2189 0.36 -1.4 2,267,714 - Synpcc7942_2189 0.36 -0.8 2,267,724 - Synpcc7942_2189 0.40 -0.9 2,267,743 - Synpcc7942_2189 0.47 -2.4 2,267,745 + Synpcc7942_2189 0.48 -0.9 2,267,745 + Synpcc7942_2189 0.48 +0.6 2,267,745 + Synpcc7942_2189 0.48 +0.9 2,267,745 + Synpcc7942_2189 0.48 +1.3 2,267,745 + Synpcc7942_2189 0.48 +0.2 2,267,753 - Synpcc7942_2189 0.51 +0.9 2,267,763 - Synpcc7942_2189 0.55 -1.2 2,267,765 - Synpcc7942_2189 0.55 -1.1 2,267,786 - Synpcc7942_2189 0.63 -0.9 2,267,866 + -1.3 2,267,867 - -3.0 2,267,874 - +2.6 2,267,876 - +0.1 2,267,916 + +1.8 2,267,916 - +0.4 2,267,922 - +1.9 2,267,925 + +1.2 2,267,933 - +0.6 2,267,979 + msrB Synpcc7942_2190 0.10 +1.7 2,267,984 + msrB Synpcc7942_2190 0.12 -0.5 2,267,984 + msrB Synpcc7942_2190 0.12 +1.2 2,267,984 + msrB Synpcc7942_2190 0.12 +1.8 2,267,984 + msrB Synpcc7942_2190 0.12 +0.9 2,267,984 + msrB Synpcc7942_2190 0.12 +1.1 2,267,984 + msrB Synpcc7942_2190 0.12 -0.2 2,267,984 + msrB Synpcc7942_2190 0.12 +2.3 2,267,984 + msrB Synpcc7942_2190 0.12 -0.3 2,267,986 + msrB Synpcc7942_2190 0.12 -0.4 2,267,992 - msrB Synpcc7942_2190 0.13 +3.9 2,268,002 - msrB Synpcc7942_2190 0.16 -1.2 2,268,047 - msrB Synpcc7942_2190 0.26 -0.7 2,268,089 + msrB Synpcc7942_2190 0.36 +3.1 2,268,091 - msrB Synpcc7942_2190 0.37 +2.8 2,268,091 - msrB Synpcc7942_2190 0.37 +0.0 2,268,102 - msrB Synpcc7942_2190 0.39 +1.1 2,268,102 - msrB Synpcc7942_2190 0.39 -2.9 2,268,107 - msrB Synpcc7942_2190 0.41 +1.2 2,268,243 + msrB Synpcc7942_2190 0.73 +3.7 2,268,280 + msrB Synpcc7942_2190 0.82 +1.8 2,268,285 + msrB Synpcc7942_2190 0.83 +2.5 2,268,285 + msrB Synpcc7942_2190 0.83 +0.4 2,268,288 - msrB Synpcc7942_2190 0.83 +0.2 2,268,307 - msrB Synpcc7942_2190 0.88 +1.0 2,268,344 + -0.0 2,268,352 + +3.8 2,268,352 + -0.9 2,268,352 + -0.8 2,268,352 + -2.2 2,268,360 - +2.5 2,268,385 + -0.9 2,268,397 + -2.4 2,268,400 + +1.4 2,268,405 - +2.1 2,268,447 + -0.8 2,268,461 + -0.6 2,268,463 + -0.1 2,268,469 - -2.0 2,268,471 - +0.2 2,268,471 - +1.6 2,268,471 - -0.4 2,268,479 - +1.1 2,268,483 + -2.9 2,268,483 + +3.8 2,268,502 - Synpcc7942_2191 0.11 -1.8 2,268,517 + Synpcc7942_2191 0.13 -1.0 2,268,544 + Synpcc7942_2191 0.17 +1.5 2,268,554 + Synpcc7942_2191 0.18 -2.8 2,268,562 + Synpcc7942_2191 0.19 -2.7 2,268,563 - Synpcc7942_2191 0.19 -1.7 2,268,570 - Synpcc7942_2191 0.20 +1.0 2,268,578 - -0.0 2,268,590 + Synpcc7942_2191 0.23 +1.1 2,268,590 + Synpcc7942_2191 0.23 -0.4 2,268,598 - Synpcc7942_2191 0.24 +1.0 2,268,598 - Synpcc7942_2191 0.24 +0.6 2,268,598 - Synpcc7942_2191 0.24 -0.0 2,268,598 - Synpcc7942_2191 0.24 -1.4 2,268,598 - Synpcc7942_2191 0.24 +0.0 2,268,598 - Synpcc7942_2191 0.24 -1.9 2,268,608 + Synpcc7942_2191 0.25 -0.9 2,268,608 + Synpcc7942_2191 0.25 -1.8 2,268,613 + Synpcc7942_2191 0.26 +1.6 2,268,613 + Synpcc7942_2191 0.26 +3.1 2,268,613 + Synpcc7942_2191 0.26 -1.9 2,268,616 + Synpcc7942_2191 0.26 +2.3 2,268,616 - Synpcc7942_2191 0.26 +0.6 2,268,616 - Synpcc7942_2191 0.26 +0.2 2,268,618 + Synpcc7942_2191 0.27 -0.9 2,268,618 + Synpcc7942_2191 0.27 -1.2 2,268,621 - Synpcc7942_2191 0.27 -1.0 2,268,621 - Synpcc7942_2191 0.27 -1.5 2,268,621 - Synpcc7942_2191 0.27 +4.4 2,268,621 - Synpcc7942_2191 0.27 -0.5 2,268,626 - Synpcc7942_2191 0.28 +0.2 2,268,626 - Synpcc7942_2191 0.28 -1.0 2,268,626 - Synpcc7942_2191 0.28 +1.5 2,268,630 + Synpcc7942_2191 0.28 +1.1 2,268,633 - Synpcc7942_2191 0.29 -2.5 2,268,652 + Synpcc7942_2191 0.31 -2.0 2,268,660 - Synpcc7942_2191 0.32 -1.4 2,268,662 + Synpcc7942_2191 0.33 -1.8 2,268,662 + Synpcc7942_2191 0.33 -0.2 2,268,686 + Synpcc7942_2191 0.36 +1.0 2,268,691 + Synpcc7942_2191 0.37 -3.3 2,268,718 - Synpcc7942_2191 0.40 +2.5 2,268,750 - Synpcc7942_2191 0.45 -1.8 2,268,755 - Synpcc7942_2191 0.45 +1.0 2,268,776 - Synpcc7942_2191 0.48 -3.4 2,268,778 + Synpcc7942_2191 0.48 +1.3 2,268,809 + Synpcc7942_2191 0.53 -2.1 2,268,809 + Synpcc7942_2191 0.53 +2.7 2,268,809 + Synpcc7942_2191 0.53 -1.3 2,268,817 - Synpcc7942_2191 0.54 +0.6 2,268,827 - Synpcc7942_2191 0.55 -0.4 2,268,852 - Synpcc7942_2191 0.59 +0.8 2,268,870 - Synpcc7942_2191 0.61 -1.4 2,268,876 - Synpcc7942_2191 0.62 -0.3
Or see this region's nucleotide sequence