Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dpsA and natD are separated by 46 nucleotides natD and Synpcc7942_2178 are separated by 20 nucleotides Synpcc7942_2178 and Synpcc7942_2179 are separated by 49 nucleotides
Synpcc7942_2176: dpsA - hypothetical protein, at 2,255,951 to 2,257,096
dpsA
Synpcc7942_2177: natD - integral membrane protein of the ABC-type Nat permease for neutral amino acids NatD, at 2,257,143 to 2,258,033
natD
Synpcc7942_2178: Synpcc7942_2178 - hypothetical protein, at 2,258,054 to 2,258,395
_2178
Synpcc7942_2179: Synpcc7942_2179 - putative lipid kinase, at 2,258,445 to 2,259,359
_2179
Position (kb)
2257
2258
2259 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2256.143 kb on - strand, within dpsA at 2256.150 kb on - strand, within dpsA at 2256.172 kb on + strand, within dpsA at 2256.180 kb on - strand, within dpsA at 2256.195 kb on + strand, within dpsA at 2256.213 kb on - strand, within dpsA at 2256.218 kb on - strand, within dpsA at 2256.232 kb on + strand, within dpsA at 2256.240 kb on - strand, within dpsA at 2256.260 kb on - strand, within dpsA at 2256.357 kb on + strand, within dpsA at 2256.360 kb on - strand, within dpsA at 2256.362 kb on + strand, within dpsA at 2256.365 kb on - strand, within dpsA at 2256.365 kb on - strand, within dpsA at 2256.406 kb on + strand, within dpsA at 2256.406 kb on + strand, within dpsA at 2256.437 kb on - strand, within dpsA at 2256.444 kb on - strand, within dpsA at 2256.475 kb on + strand, within dpsA at 2256.483 kb on - strand, within dpsA at 2256.483 kb on - strand, within dpsA at 2256.485 kb on - strand, within dpsA at 2256.527 kb on - strand, within dpsA at 2256.576 kb on - strand, within dpsA at 2256.578 kb on + strand at 2256.578 kb on + strand, within dpsA at 2256.578 kb on + strand at 2256.586 kb on + strand, within dpsA at 2256.586 kb on - strand, within dpsA at 2256.586 kb on - strand, within dpsA at 2256.586 kb on - strand, within dpsA at 2256.600 kb on + strand, within dpsA at 2256.600 kb on + strand, within dpsA at 2256.600 kb on + strand, within dpsA at 2256.600 kb on + strand, within dpsA at 2256.600 kb on + strand, within dpsA at 2256.608 kb on - strand, within dpsA at 2256.608 kb on - strand, within dpsA at 2256.608 kb on - strand, within dpsA at 2256.608 kb on - strand, within dpsA at 2256.609 kb on - strand, within dpsA at 2256.615 kb on + strand, within dpsA at 2256.615 kb on + strand, within dpsA at 2256.628 kb on - strand, within dpsA at 2256.630 kb on - strand, within dpsA at 2256.646 kb on + strand, within dpsA at 2256.652 kb on - strand, within dpsA at 2256.653 kb on - strand, within dpsA at 2256.676 kb on - strand, within dpsA at 2256.728 kb on - strand, within dpsA at 2256.800 kb on + strand, within dpsA at 2256.818 kb on + strand, within dpsA at 2256.839 kb on - strand, within dpsA at 2256.874 kb on - strand, within dpsA at 2256.887 kb on + strand, within dpsA at 2256.890 kb on - strand, within dpsA at 2256.890 kb on - strand, within dpsA at 2256.915 kb on + strand, within dpsA at 2256.972 kb on - strand, within dpsA at 2256.974 kb on - strand, within dpsA at 2256.991 kb on - strand at 2256.991 kb on - strand at 2257.000 kb on + strand at 2257.008 kb on - strand at 2257.042 kb on + strand at 2257.050 kb on - strand at 2257.053 kb on + strand at 2257.064 kb on + strand at 2257.072 kb on - strand at 2257.131 kb on + strand at 2257.138 kb on - strand at 2257.167 kb on + strand at 2257.167 kb on + strand at 2257.168 kb on + strand at 2257.168 kb on + strand at 2257.176 kb on - strand at 2257.177 kb on + strand at 2257.185 kb on - strand at 2257.197 kb on + strand at 2257.207 kb on - strand at 2257.224 kb on + strand at 2257.232 kb on - strand, within natD at 2257.232 kb on - strand, within natD at 2257.232 kb on - strand, within natD at 2257.320 kb on - strand, within natD at 2257.339 kb on + strand, within natD at 2257.347 kb on - strand, within natD at 2257.351 kb on + strand, within natD at 2257.351 kb on + strand, within natD at 2257.359 kb on - strand, within natD at 2257.359 kb on - strand, within natD at 2257.359 kb on - strand, within natD at 2257.420 kb on + strand at 2257.424 kb on - strand, within natD at 2257.474 kb on + strand, within natD at 2257.474 kb on + strand, within natD at 2257.474 kb on + strand, within natD at 2257.482 kb on - strand, within natD at 2257.560 kb on + strand, within natD at 2257.560 kb on + strand, within natD at 2257.561 kb on + strand, within natD at 2257.610 kb on + strand, within natD at 2257.640 kb on + strand, within natD at 2257.663 kb on - strand, within natD at 2257.663 kb on - strand, within natD at 2257.667 kb on + strand, within natD at 2257.667 kb on + strand, within natD at 2257.702 kb on + strand, within natD at 2257.710 kb on - strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.720 kb on - strand, within natD at 2257.720 kb on - strand, within natD at 2257.720 kb on - strand, within natD at 2257.720 kb on - strand, within natD at 2257.720 kb on - strand, within natD at 2257.735 kb on + strand, within natD at 2257.768 kb on + strand, within natD at 2257.768 kb on + strand, within natD at 2257.798 kb on - strand, within natD at 2257.873 kb on + strand, within natD at 2257.880 kb on - strand, within natD at 2257.883 kb on + strand, within natD at 2257.883 kb on + strand, within natD at 2257.891 kb on - strand, within natD at 2257.891 kb on - strand, within natD at 2257.895 kb on + strand, within natD at 2257.931 kb on + strand, within natD at 2258.016 kb on + strand at 2258.019 kb on - strand at 2258.111 kb on - strand, within Synpcc7942_2178 at 2258.119 kb on - strand, within Synpcc7942_2178 at 2258.179 kb on + strand, within Synpcc7942_2178 at 2258.191 kb on - strand, within Synpcc7942_2178 at 2258.206 kb on + strand, within Synpcc7942_2178 at 2258.221 kb on - strand, within Synpcc7942_2178 at 2258.268 kb on - strand, within Synpcc7942_2178 at 2258.282 kb on + strand, within Synpcc7942_2178 at 2258.282 kb on + strand, within Synpcc7942_2178 at 2258.285 kb on - strand, within Synpcc7942_2178 at 2258.336 kb on - strand, within Synpcc7942_2178 at 2258.410 kb on + strand at 2258.425 kb on - strand at 2258.450 kb on + strand at 2258.450 kb on + strand at 2258.452 kb on + strand at 2258.458 kb on - strand at 2258.460 kb on - strand at 2258.460 kb on - strand at 2258.462 kb on + strand at 2258.511 kb on + strand at 2258.522 kb on + strand at 2258.522 kb on + strand at 2258.537 kb on - strand, within Synpcc7942_2179 at 2258.538 kb on - strand, within Synpcc7942_2179 at 2258.579 kb on + strand, within Synpcc7942_2179 at 2258.599 kb on - strand, within Synpcc7942_2179 at 2258.601 kb on + strand, within Synpcc7942_2179 at 2258.623 kb on + strand, within Synpcc7942_2179 at 2258.631 kb on - strand, within Synpcc7942_2179 at 2258.638 kb on - strand, within Synpcc7942_2179 at 2258.657 kb on + strand, within Synpcc7942_2179 at 2258.705 kb on + strand, within Synpcc7942_2179 at 2258.744 kb on - strand, within Synpcc7942_2179 at 2258.793 kb on - strand, within Synpcc7942_2179 at 2258.793 kb on - strand, within Synpcc7942_2179 at 2258.874 kb on + strand, within Synpcc7942_2179 at 2258.874 kb on + strand, within Synpcc7942_2179 at 2258.874 kb on + strand, within Synpcc7942_2179 at 2258.874 kb on + strand, within Synpcc7942_2179 at 2258.882 kb on - strand, within Synpcc7942_2179 at 2258.882 kb on - strand, within Synpcc7942_2179 at 2258.882 kb on - strand, within Synpcc7942_2179 at 2258.884 kb on + strand, within Synpcc7942_2179 at 2258.887 kb on - strand, within Synpcc7942_2179 at 2258.892 kb on - strand, within Synpcc7942_2179 at 2258.892 kb on - strand, within Synpcc7942_2179 at 2258.907 kb on + strand, within Synpcc7942_2179 at 2258.908 kb on + strand, within Synpcc7942_2179 at 2258.908 kb on + strand, within Synpcc7942_2179 at 2258.915 kb on - strand, within Synpcc7942_2179 at 2258.916 kb on - strand, within Synpcc7942_2179 at 2258.968 kb on + strand, within Synpcc7942_2179 at 2258.982 kb on - strand, within Synpcc7942_2179 at 2258.997 kb on + strand, within Synpcc7942_2179 at 2259.005 kb on - strand, within Synpcc7942_2179 at 2259.009 kb on + strand, within Synpcc7942_2179 at 2259.009 kb on + strand, within Synpcc7942_2179 at 2259.009 kb on + strand, within Synpcc7942_2179
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,256,143 - dpsA Synpcc7942_2176 0.17 +0.6 2,256,150 - dpsA Synpcc7942_2176 0.17 -1.0 2,256,172 + dpsA Synpcc7942_2176 0.19 -0.6 2,256,180 - dpsA Synpcc7942_2176 0.20 -1.1 2,256,195 + dpsA Synpcc7942_2176 0.21 +1.5 2,256,213 - dpsA Synpcc7942_2176 0.23 -1.3 2,256,218 - dpsA Synpcc7942_2176 0.23 -1.3 2,256,232 + dpsA Synpcc7942_2176 0.25 -2.0 2,256,240 - dpsA Synpcc7942_2176 0.25 -4.2 2,256,260 - dpsA Synpcc7942_2176 0.27 -0.9 2,256,357 + dpsA Synpcc7942_2176 0.35 -3.0 2,256,360 - dpsA Synpcc7942_2176 0.36 +1.2 2,256,362 + dpsA Synpcc7942_2176 0.36 -1.0 2,256,365 - dpsA Synpcc7942_2176 0.36 -1.6 2,256,365 - dpsA Synpcc7942_2176 0.36 -1.3 2,256,406 + dpsA Synpcc7942_2176 0.40 +0.6 2,256,406 + dpsA Synpcc7942_2176 0.40 -1.6 2,256,437 - dpsA Synpcc7942_2176 0.42 -1.0 2,256,444 - dpsA Synpcc7942_2176 0.43 -1.7 2,256,475 + dpsA Synpcc7942_2176 0.46 +1.3 2,256,483 - dpsA Synpcc7942_2176 0.46 -2.0 2,256,483 - dpsA Synpcc7942_2176 0.46 +0.2 2,256,485 - dpsA Synpcc7942_2176 0.47 +0.4 2,256,527 - dpsA Synpcc7942_2176 0.50 -2.5 2,256,576 - dpsA Synpcc7942_2176 0.55 -2.1 2,256,578 + +1.0 2,256,578 + dpsA Synpcc7942_2176 0.55 -0.3 2,256,578 + +0.2 2,256,586 + dpsA Synpcc7942_2176 0.55 +2.1 2,256,586 - dpsA Synpcc7942_2176 0.55 -1.2 2,256,586 - dpsA Synpcc7942_2176 0.55 -1.1 2,256,586 - dpsA Synpcc7942_2176 0.55 -1.3 2,256,600 + dpsA Synpcc7942_2176 0.57 -0.0 2,256,600 + dpsA Synpcc7942_2176 0.57 -0.4 2,256,600 + dpsA Synpcc7942_2176 0.57 +0.5 2,256,600 + dpsA Synpcc7942_2176 0.57 +0.1 2,256,600 + dpsA Synpcc7942_2176 0.57 -0.1 2,256,608 - dpsA Synpcc7942_2176 0.57 -1.2 2,256,608 - dpsA Synpcc7942_2176 0.57 +0.9 2,256,608 - dpsA Synpcc7942_2176 0.57 -1.2 2,256,608 - dpsA Synpcc7942_2176 0.57 -1.7 2,256,609 - dpsA Synpcc7942_2176 0.57 -2.5 2,256,615 + dpsA Synpcc7942_2176 0.58 -0.2 2,256,615 + dpsA Synpcc7942_2176 0.58 +0.5 2,256,628 - dpsA Synpcc7942_2176 0.59 +1.7 2,256,630 - dpsA Synpcc7942_2176 0.59 -0.2 2,256,646 + dpsA Synpcc7942_2176 0.61 +0.3 2,256,652 - dpsA Synpcc7942_2176 0.61 +0.6 2,256,653 - dpsA Synpcc7942_2176 0.61 -0.1 2,256,676 - dpsA Synpcc7942_2176 0.63 +0.7 2,256,728 - dpsA Synpcc7942_2176 0.68 +1.1 2,256,800 + dpsA Synpcc7942_2176 0.74 -2.4 2,256,818 + dpsA Synpcc7942_2176 0.76 -0.2 2,256,839 - dpsA Synpcc7942_2176 0.77 -1.3 2,256,874 - dpsA Synpcc7942_2176 0.81 -0.8 2,256,887 + dpsA Synpcc7942_2176 0.82 +2.9 2,256,890 - dpsA Synpcc7942_2176 0.82 +0.2 2,256,890 - dpsA Synpcc7942_2176 0.82 -0.2 2,256,915 + dpsA Synpcc7942_2176 0.84 -1.0 2,256,972 - dpsA Synpcc7942_2176 0.89 -0.3 2,256,974 - dpsA Synpcc7942_2176 0.89 -1.7 2,256,991 - +0.1 2,256,991 - +1.5 2,257,000 + -2.7 2,257,008 - +1.5 2,257,042 + +0.7 2,257,050 - -2.4 2,257,053 + -2.4 2,257,064 + -2.0 2,257,072 - -1.3 2,257,131 + -0.1 2,257,138 - +1.0 2,257,167 + -0.6 2,257,167 + +1.7 2,257,168 + -0.6 2,257,168 + -0.5 2,257,176 - +1.5 2,257,177 + +2.7 2,257,185 - -1.7 2,257,197 + +1.1 2,257,207 - -1.2 2,257,224 + +0.6 2,257,232 - natD Synpcc7942_2177 0.10 +1.4 2,257,232 - natD Synpcc7942_2177 0.10 -0.8 2,257,232 - natD Synpcc7942_2177 0.10 +1.7 2,257,320 - natD Synpcc7942_2177 0.20 +1.1 2,257,339 + natD Synpcc7942_2177 0.22 +1.7 2,257,347 - natD Synpcc7942_2177 0.23 +0.7 2,257,351 + natD Synpcc7942_2177 0.23 -0.0 2,257,351 + natD Synpcc7942_2177 0.23 +0.9 2,257,359 - natD Synpcc7942_2177 0.24 -0.7 2,257,359 - natD Synpcc7942_2177 0.24 +2.4 2,257,359 - natD Synpcc7942_2177 0.24 +2.6 2,257,420 + +5.1 2,257,424 - natD Synpcc7942_2177 0.32 -1.5 2,257,474 + natD Synpcc7942_2177 0.37 +1.4 2,257,474 + natD Synpcc7942_2177 0.37 +0.9 2,257,474 + natD Synpcc7942_2177 0.37 -0.5 2,257,482 - natD Synpcc7942_2177 0.38 +2.0 2,257,560 + natD Synpcc7942_2177 0.47 +1.4 2,257,560 + natD Synpcc7942_2177 0.47 -0.0 2,257,561 + natD Synpcc7942_2177 0.47 -1.7 2,257,610 + natD Synpcc7942_2177 0.52 +3.6 2,257,640 + natD Synpcc7942_2177 0.56 -0.7 2,257,663 - natD Synpcc7942_2177 0.58 -1.5 2,257,663 - natD Synpcc7942_2177 0.58 -0.9 2,257,667 + natD Synpcc7942_2177 0.59 -0.8 2,257,667 + natD Synpcc7942_2177 0.59 +1.5 2,257,702 + natD Synpcc7942_2177 0.63 -0.7 2,257,710 - natD Synpcc7942_2177 0.64 +1.0 2,257,712 + natD Synpcc7942_2177 0.64 -0.0 2,257,712 + natD Synpcc7942_2177 0.64 +2.2 2,257,712 + natD Synpcc7942_2177 0.64 +2.1 2,257,712 + natD Synpcc7942_2177 0.64 -0.8 2,257,712 + natD Synpcc7942_2177 0.64 +0.7 2,257,712 + natD Synpcc7942_2177 0.64 +1.3 2,257,712 + natD Synpcc7942_2177 0.64 +2.8 2,257,712 + natD Synpcc7942_2177 0.64 +1.6 2,257,712 + natD Synpcc7942_2177 0.64 -0.6 2,257,720 - natD Synpcc7942_2177 0.65 -2.9 2,257,720 - natD Synpcc7942_2177 0.65 -1.5 2,257,720 - natD Synpcc7942_2177 0.65 -2.3 2,257,720 - natD Synpcc7942_2177 0.65 -1.7 2,257,720 - natD Synpcc7942_2177 0.65 +4.7 2,257,735 + natD Synpcc7942_2177 0.66 -0.0 2,257,768 + natD Synpcc7942_2177 0.70 -0.7 2,257,768 + natD Synpcc7942_2177 0.70 +2.9 2,257,798 - natD Synpcc7942_2177 0.74 +1.0 2,257,873 + natD Synpcc7942_2177 0.82 +1.6 2,257,880 - natD Synpcc7942_2177 0.83 -0.2 2,257,883 + natD Synpcc7942_2177 0.83 +1.7 2,257,883 + natD Synpcc7942_2177 0.83 -0.8 2,257,891 - natD Synpcc7942_2177 0.84 +0.9 2,257,891 - natD Synpcc7942_2177 0.84 +2.8 2,257,895 + natD Synpcc7942_2177 0.84 +1.6 2,257,931 + natD Synpcc7942_2177 0.88 +1.4 2,258,016 + -0.0 2,258,019 - +2.3 2,258,111 - Synpcc7942_2178 0.17 +2.2 2,258,119 - Synpcc7942_2178 0.19 -0.4 2,258,179 + Synpcc7942_2178 0.37 -1.0 2,258,191 - Synpcc7942_2178 0.40 -1.7 2,258,206 + Synpcc7942_2178 0.44 +2.0 2,258,221 - Synpcc7942_2178 0.49 +3.6 2,258,268 - Synpcc7942_2178 0.63 +2.8 2,258,282 + Synpcc7942_2178 0.67 -2.7 2,258,282 + Synpcc7942_2178 0.67 -2.3 2,258,285 - Synpcc7942_2178 0.68 +0.8 2,258,336 - Synpcc7942_2178 0.82 -0.3 2,258,410 + -2.7 2,258,425 - -1.7 2,258,450 + +1.8 2,258,450 + +0.9 2,258,452 + -3.1 2,258,458 - +3.9 2,258,460 - -3.0 2,258,460 - +3.4 2,258,462 + +0.8 2,258,511 + -1.0 2,258,522 + -2.0 2,258,522 + +0.3 2,258,537 - Synpcc7942_2179 0.10 +2.5 2,258,538 - Synpcc7942_2179 0.10 +0.5 2,258,579 + Synpcc7942_2179 0.15 +1.6 2,258,599 - Synpcc7942_2179 0.17 -1.5 2,258,601 + Synpcc7942_2179 0.17 +3.0 2,258,623 + Synpcc7942_2179 0.19 +0.5 2,258,631 - Synpcc7942_2179 0.20 -0.6 2,258,638 - Synpcc7942_2179 0.21 -0.7 2,258,657 + Synpcc7942_2179 0.23 +3.4 2,258,705 + Synpcc7942_2179 0.28 -0.8 2,258,744 - Synpcc7942_2179 0.33 -1.8 2,258,793 - Synpcc7942_2179 0.38 -0.7 2,258,793 - Synpcc7942_2179 0.38 -3.2 2,258,874 + Synpcc7942_2179 0.47 -1.9 2,258,874 + Synpcc7942_2179 0.47 -3.3 2,258,874 + Synpcc7942_2179 0.47 -1.9 2,258,874 + Synpcc7942_2179 0.47 +2.5 2,258,882 - Synpcc7942_2179 0.48 -2.1 2,258,882 - Synpcc7942_2179 0.48 +2.0 2,258,882 - Synpcc7942_2179 0.48 -3.0 2,258,884 + Synpcc7942_2179 0.48 -2.2 2,258,887 - Synpcc7942_2179 0.48 +1.1 2,258,892 - Synpcc7942_2179 0.49 -1.3 2,258,892 - Synpcc7942_2179 0.49 -1.3 2,258,907 + Synpcc7942_2179 0.50 -1.0 2,258,908 + Synpcc7942_2179 0.51 -2.8 2,258,908 + Synpcc7942_2179 0.51 +2.6 2,258,915 - Synpcc7942_2179 0.51 +5.3 2,258,916 - Synpcc7942_2179 0.51 -1.8 2,258,968 + Synpcc7942_2179 0.57 +1.9 2,258,982 - Synpcc7942_2179 0.59 +5.3 2,258,997 + Synpcc7942_2179 0.60 -1.5 2,259,005 - Synpcc7942_2179 0.61 -0.3 2,259,009 + Synpcc7942_2179 0.62 +1.4 2,259,009 + Synpcc7942_2179 0.62 -1.5 2,259,009 + Synpcc7942_2179 0.62 -2.2
Or see this region's nucleotide sequence