Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpB and Synpcc7942_2144 are separated by 358 nucleotides Synpcc7942_2144 and Synpcc7942_2145 are separated by 5 nucleotides Synpcc7942_2145 and Synpcc7942_2146 are separated by 98 nucleotides
Synpcc7942_2143: trpB - tryptophan synthase subunit beta, at 2,226,908 to 2,228,170
trpB
Synpcc7942_2144: Synpcc7942_2144 - nuclease (SNase-like), at 2,228,529 to 2,229,017
_2144
Synpcc7942_2145: Synpcc7942_2145 - hypothetical protein, at 2,229,023 to 2,229,910
_2145
Synpcc7942_2146: Synpcc7942_2146 - hypothetical protein, at 2,230,009 to 2,230,323
_2146
Position (kb)
2228
2229
2230 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8
9
10
11 at 2228.180 kb on + strand at 2228.215 kb on - strand at 2228.215 kb on - strand at 2228.222 kb on + strand at 2228.254 kb on - strand at 2228.259 kb on - strand at 2228.276 kb on - strand at 2228.314 kb on + strand at 2228.320 kb on - strand at 2228.322 kb on + strand at 2228.365 kb on + strand at 2228.368 kb on - strand at 2228.368 kb on - strand at 2228.381 kb on - strand at 2228.446 kb on - strand at 2228.452 kb on + strand at 2228.453 kb on + strand at 2228.461 kb on - strand at 2228.499 kb on + strand at 2228.499 kb on + strand at 2228.504 kb on + strand at 2228.507 kb on - strand at 2228.521 kb on + strand at 2228.526 kb on + strand at 2228.534 kb on + strand at 2228.534 kb on - strand at 2228.554 kb on - strand at 2228.591 kb on - strand, within Synpcc7942_2144 at 2228.612 kb on - strand, within Synpcc7942_2144 at 2228.628 kb on + strand, within Synpcc7942_2144 at 2228.743 kb on - strand, within Synpcc7942_2144 at 2228.743 kb on - strand at 2228.750 kb on - strand, within Synpcc7942_2144 at 2228.750 kb on - strand, within Synpcc7942_2144 at 2228.763 kb on + strand, within Synpcc7942_2144 at 2228.784 kb on + strand, within Synpcc7942_2144 at 2228.793 kb on + strand, within Synpcc7942_2144 at 2228.817 kb on + strand, within Synpcc7942_2144 at 2228.840 kb on + strand, within Synpcc7942_2144 at 2228.845 kb on + strand, within Synpcc7942_2144 at 2228.845 kb on + strand at 2228.848 kb on - strand, within Synpcc7942_2144 at 2228.853 kb on - strand, within Synpcc7942_2144 at 2228.859 kb on + strand, within Synpcc7942_2144 at 2228.895 kb on + strand, within Synpcc7942_2144 at 2228.905 kb on + strand, within Synpcc7942_2144 at 2228.905 kb on + strand, within Synpcc7942_2144 at 2228.913 kb on - strand, within Synpcc7942_2144 at 2228.913 kb on - strand, within Synpcc7942_2144 at 2228.913 kb on - strand, within Synpcc7942_2144 at 2228.913 kb on - strand, within Synpcc7942_2144 at 2228.934 kb on + strand, within Synpcc7942_2144 at 2228.962 kb on - strand, within Synpcc7942_2144 at 2228.978 kb on + strand at 2229.007 kb on + strand at 2229.014 kb on + strand at 2229.028 kb on - strand at 2229.251 kb on - strand, within Synpcc7942_2145 at 2229.340 kb on - strand, within Synpcc7942_2145 at 2229.434 kb on - strand, within Synpcc7942_2145 at 2229.646 kb on + strand, within Synpcc7942_2145 at 2229.687 kb on + strand, within Synpcc7942_2145 at 2229.697 kb on - strand, within Synpcc7942_2145 at 2229.697 kb on - strand, within Synpcc7942_2145 at 2229.914 kb on - strand at 2229.916 kb on + strand at 2229.923 kb on + strand at 2229.926 kb on - strand at 2229.931 kb on - strand at 2229.934 kb on - strand at 2229.936 kb on - strand at 2229.970 kb on + strand at 2229.997 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,228,180 + -0.6 2,228,215 - -0.2 2,228,215 - -0.0 2,228,222 + +1.4 2,228,254 - +1.4 2,228,259 - +0.4 2,228,276 - +0.7 2,228,314 + -1.5 2,228,320 - -0.5 2,228,322 + +2.4 2,228,365 + -2.2 2,228,368 - +0.0 2,228,368 - +4.1 2,228,381 - +1.9 2,228,446 - -1.9 2,228,452 + -1.0 2,228,453 + +3.6 2,228,461 - +2.4 2,228,499 + +0.3 2,228,499 + +3.2 2,228,504 + +2.9 2,228,507 - +3.6 2,228,521 + +1.9 2,228,526 + -0.6 2,228,534 + +5.6 2,228,534 - -2.5 2,228,554 - +9.8 2,228,591 - Synpcc7942_2144 0.13 +7.2 2,228,612 - Synpcc7942_2144 0.17 +9.0 2,228,628 + Synpcc7942_2144 0.20 +9.0 2,228,743 - Synpcc7942_2144 0.44 +11.1 2,228,743 - +9.1 2,228,750 - Synpcc7942_2144 0.45 +7.2 2,228,750 - Synpcc7942_2144 0.45 +7.4 2,228,763 + Synpcc7942_2144 0.48 +9.3 2,228,784 + Synpcc7942_2144 0.52 +8.4 2,228,793 + Synpcc7942_2144 0.54 +4.6 2,228,817 + Synpcc7942_2144 0.59 +6.9 2,228,840 + Synpcc7942_2144 0.64 +9.4 2,228,845 + Synpcc7942_2144 0.65 +8.1 2,228,845 + +4.4 2,228,848 - Synpcc7942_2144 0.65 +8.6 2,228,853 - Synpcc7942_2144 0.66 +10.5 2,228,859 + Synpcc7942_2144 0.67 +8.4 2,228,895 + Synpcc7942_2144 0.75 +9.3 2,228,905 + Synpcc7942_2144 0.77 +7.3 2,228,905 + Synpcc7942_2144 0.77 +8.2 2,228,913 - Synpcc7942_2144 0.79 +9.4 2,228,913 - Synpcc7942_2144 0.79 +9.1 2,228,913 - Synpcc7942_2144 0.79 +8.3 2,228,913 - Synpcc7942_2144 0.79 +9.4 2,228,934 + Synpcc7942_2144 0.83 +9.2 2,228,962 - Synpcc7942_2144 0.89 +8.2 2,228,978 + +9.4 2,229,007 + +9.3 2,229,014 + +2.7 2,229,028 - +8.6 2,229,251 - Synpcc7942_2145 0.26 -3.2 2,229,340 - Synpcc7942_2145 0.36 +0.0 2,229,434 - Synpcc7942_2145 0.46 -0.3 2,229,646 + Synpcc7942_2145 0.70 -1.8 2,229,687 + Synpcc7942_2145 0.75 -1.5 2,229,697 - Synpcc7942_2145 0.76 -1.2 2,229,697 - Synpcc7942_2145 0.76 -1.8 2,229,914 - -1.0 2,229,916 + -4.1 2,229,923 + -1.4 2,229,926 - -4.7 2,229,931 - -2.9 2,229,934 - +1.6 2,229,936 - +2.6 2,229,970 + -0.5 2,229,997 - -0.2
Or see this region's nucleotide sequence