Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt slt and manB are separated by 293 nucleotides manB and Synpcc7942_2133 are separated by 2 nucleotides Synpcc7942_2133 and amiA are separated by 182 nucleotides
Synpcc7942_2131: slt - probable soluble lytic transglycosylase, at 2,209,183 to 2,211,255
slt
Synpcc7942_2132: manB - phosphoglucosamine mutase, at 2,211,549 to 2,212,967
manB
Synpcc7942_2133: Synpcc7942_2133 - hypothetical protein, at 2,212,970 to 2,213,476
_2133
Synpcc7942_2134: amiA - Cell wall hydrolase/autolysin, at 2,213,659 to 2,214,708
amiA
Position (kb)
2211
2212
2213 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 2210.556 kb on - strand, within slt at 2210.559 kb on + strand, within slt at 2210.583 kb on + strand, within slt at 2210.600 kb on + strand, within slt at 2210.620 kb on - strand, within slt at 2210.639 kb on + strand, within slt at 2210.727 kb on + strand, within slt at 2210.735 kb on - strand, within slt at 2210.787 kb on + strand, within slt at 2210.787 kb on + strand, within slt at 2210.797 kb on - strand, within slt at 2210.804 kb on + strand, within slt at 2210.812 kb on - strand, within slt at 2210.814 kb on + strand, within slt at 2210.814 kb on + strand, within slt at 2210.817 kb on - strand, within slt at 2210.834 kb on + strand, within slt at 2210.834 kb on + strand, within slt at 2210.834 kb on + strand, within slt at 2210.834 kb on + strand, within slt at 2210.842 kb on - strand, within slt at 2210.848 kb on + strand, within slt at 2210.872 kb on + strand, within slt at 2210.899 kb on + strand, within slt at 2210.899 kb on + strand, within slt at 2210.899 kb on + strand, within slt at 2210.909 kb on - strand, within slt at 2210.910 kb on + strand, within slt at 2210.915 kb on - strand, within slt at 2210.923 kb on - strand, within slt at 2210.927 kb on + strand, within slt at 2210.932 kb on + strand, within slt at 2210.933 kb on - strand, within slt at 2210.937 kb on + strand, within slt at 2210.937 kb on + strand, within slt at 2210.945 kb on - strand, within slt at 2210.945 kb on - strand, within slt at 2210.945 kb on - strand, within slt at 2210.955 kb on - strand, within slt at 2210.966 kb on + strand, within slt at 2210.971 kb on + strand, within slt at 2210.976 kb on + strand, within slt at 2210.976 kb on + strand, within slt at 2210.976 kb on + strand, within slt at 2210.979 kb on - strand, within slt at 2210.984 kb on - strand, within slt at 2210.984 kb on - strand, within slt at 2210.989 kb on + strand, within slt at 2211.013 kb on + strand, within slt at 2211.031 kb on + strand, within slt at 2211.055 kb on + strand at 2211.055 kb on + strand at 2211.065 kb on + strand at 2211.096 kb on + strand at 2211.096 kb on + strand at 2211.263 kb on + strand at 2211.263 kb on + strand at 2211.271 kb on - strand at 2211.304 kb on - strand at 2211.307 kb on - strand at 2211.310 kb on + strand at 2211.374 kb on - strand at 2211.395 kb on - strand at 2211.395 kb on - strand at 2211.422 kb on + strand at 2211.422 kb on + strand at 2211.423 kb on - strand at 2211.423 kb on - strand at 2211.442 kb on + strand at 2211.450 kb on - strand at 2211.453 kb on - strand at 2211.465 kb on + strand at 2211.495 kb on - strand at 2211.534 kb on + strand at 2211.540 kb on - strand at 2211.540 kb on - strand at 2211.542 kb on - strand at 2211.547 kb on - strand at 2211.591 kb on + strand at 2211.599 kb on - strand at 2211.626 kb on + strand at 2211.730 kb on - strand, within manB at 2211.730 kb on - strand, within manB at 2211.799 kb on + strand, within manB at 2211.891 kb on - strand, within manB at 2212.053 kb on + strand, within manB at 2212.204 kb on + strand, within manB at 2212.370 kb on + strand, within manB at 2212.605 kb on - strand, within manB at 2212.794 kb on - strand, within manB at 2212.799 kb on - strand, within manB at 2212.964 kb on - strand at 2212.968 kb on + strand at 2212.969 kb on - strand at 2212.969 kb on - strand at 2212.997 kb on + strand at 2213.007 kb on + strand at 2213.013 kb on + strand at 2213.015 kb on - strand at 2213.057 kb on + strand, within Synpcc7942_2133 at 2213.060 kb on - strand, within Synpcc7942_2133 at 2213.063 kb on + strand, within Synpcc7942_2133 at 2213.071 kb on - strand, within Synpcc7942_2133 at 2213.093 kb on + strand, within Synpcc7942_2133 at 2213.175 kb on + strand, within Synpcc7942_2133 at 2213.175 kb on + strand, within Synpcc7942_2133 at 2213.183 kb on - strand, within Synpcc7942_2133 at 2213.195 kb on - strand, within Synpcc7942_2133 at 2213.203 kb on - strand, within Synpcc7942_2133 at 2213.203 kb on - strand, within Synpcc7942_2133 at 2213.207 kb on - strand, within Synpcc7942_2133 at 2213.246 kb on + strand, within Synpcc7942_2133 at 2213.259 kb on - strand, within Synpcc7942_2133 at 2213.269 kb on - strand, within Synpcc7942_2133 at 2213.272 kb on - strand, within Synpcc7942_2133 at 2213.276 kb on - strand, within Synpcc7942_2133 at 2213.281 kb on - strand, within Synpcc7942_2133 at 2213.375 kb on + strand, within Synpcc7942_2133 at 2213.375 kb on + strand, within Synpcc7942_2133 at 2213.375 kb on + strand, within Synpcc7942_2133 at 2213.375 kb on + strand, within Synpcc7942_2133 at 2213.375 kb on + strand, within Synpcc7942_2133 at 2213.380 kb on + strand, within Synpcc7942_2133 at 2213.383 kb on - strand, within Synpcc7942_2133 at 2213.383 kb on - strand, within Synpcc7942_2133 at 2213.383 kb on - strand, within Synpcc7942_2133 at 2213.384 kb on + strand, within Synpcc7942_2133 at 2213.385 kb on + strand, within Synpcc7942_2133 at 2213.385 kb on + strand, within Synpcc7942_2133 at 2213.393 kb on - strand, within Synpcc7942_2133 at 2213.393 kb on - strand, within Synpcc7942_2133 at 2213.393 kb on - strand, within Synpcc7942_2133 at 2213.405 kb on + strand, within Synpcc7942_2133 at 2213.423 kb on - strand, within Synpcc7942_2133 at 2213.425 kb on + strand, within Synpcc7942_2133 at 2213.437 kb on - strand at 2213.444 kb on - strand at 2213.507 kb on - strand at 2213.507 kb on - strand at 2213.525 kb on - strand at 2213.545 kb on + strand at 2213.590 kb on + strand at 2213.590 kb on - strand at 2213.598 kb on - strand at 2213.605 kb on - strand at 2213.624 kb on - strand at 2213.624 kb on - strand at 2213.639 kb on + strand at 2213.639 kb on + strand at 2213.647 kb on - strand at 2213.647 kb on - strand at 2213.647 kb on - strand at 2213.650 kb on + strand at 2213.652 kb on - strand at 2213.682 kb on - strand at 2213.709 kb on - strand at 2213.752 kb on - strand at 2213.753 kb on - strand at 2213.787 kb on - strand, within amiA at 2213.842 kb on - strand, within amiA at 2213.854 kb on + strand, within amiA at 2213.854 kb on + strand, within amiA at 2213.862 kb on - strand, within amiA at 2213.877 kb on - strand, within amiA at 2213.877 kb on - strand, within amiA at 2213.877 kb on - strand, within amiA at 2213.877 kb on - strand, within amiA at 2213.897 kb on - strand, within amiA at 2213.918 kb on + strand, within amiA at 2213.922 kb on - strand, within amiA at 2213.929 kb on + strand, within amiA at 2213.934 kb on + strand, within amiA at 2213.953 kb on - strand, within amiA
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,210,556 - slt Synpcc7942_2131 0.66 -3.7 2,210,559 + slt Synpcc7942_2131 0.66 -1.2 2,210,583 + slt Synpcc7942_2131 0.68 -3.7 2,210,600 + slt Synpcc7942_2131 0.68 -4.6 2,210,620 - slt Synpcc7942_2131 0.69 -2.6 2,210,639 + slt Synpcc7942_2131 0.70 -1.8 2,210,727 + slt Synpcc7942_2131 0.74 -1.4 2,210,735 - slt Synpcc7942_2131 0.75 -3.0 2,210,787 + slt Synpcc7942_2131 0.77 -2.6 2,210,787 + slt Synpcc7942_2131 0.77 -1.3 2,210,797 - slt Synpcc7942_2131 0.78 -1.4 2,210,804 + slt Synpcc7942_2131 0.78 -0.6 2,210,812 - slt Synpcc7942_2131 0.79 -3.0 2,210,814 + slt Synpcc7942_2131 0.79 -3.9 2,210,814 + slt Synpcc7942_2131 0.79 -4.7 2,210,817 - slt Synpcc7942_2131 0.79 -2.5 2,210,834 + slt Synpcc7942_2131 0.80 -4.0 2,210,834 + slt Synpcc7942_2131 0.80 -2.3 2,210,834 + slt Synpcc7942_2131 0.80 -5.1 2,210,834 + slt Synpcc7942_2131 0.80 -0.8 2,210,842 - slt Synpcc7942_2131 0.80 -1.0 2,210,848 + slt Synpcc7942_2131 0.80 -2.8 2,210,872 + slt Synpcc7942_2131 0.81 -3.9 2,210,899 + slt Synpcc7942_2131 0.83 -1.7 2,210,899 + slt Synpcc7942_2131 0.83 -2.7 2,210,899 + slt Synpcc7942_2131 0.83 -3.2 2,210,909 - slt Synpcc7942_2131 0.83 -2.6 2,210,910 + slt Synpcc7942_2131 0.83 -2.7 2,210,915 - slt Synpcc7942_2131 0.84 +0.0 2,210,923 - slt Synpcc7942_2131 0.84 -3.1 2,210,927 + slt Synpcc7942_2131 0.84 -0.6 2,210,932 + slt Synpcc7942_2131 0.84 -4.4 2,210,933 - slt Synpcc7942_2131 0.84 -1.0 2,210,937 + slt Synpcc7942_2131 0.85 -3.8 2,210,937 + slt Synpcc7942_2131 0.85 -2.4 2,210,945 - slt Synpcc7942_2131 0.85 -2.3 2,210,945 - slt Synpcc7942_2131 0.85 -2.2 2,210,945 - slt Synpcc7942_2131 0.85 -1.0 2,210,955 - slt Synpcc7942_2131 0.85 -3.2 2,210,966 + slt Synpcc7942_2131 0.86 -2.4 2,210,971 + slt Synpcc7942_2131 0.86 -5.9 2,210,976 + slt Synpcc7942_2131 0.86 -1.0 2,210,976 + slt Synpcc7942_2131 0.86 -3.9 2,210,976 + slt Synpcc7942_2131 0.86 -1.8 2,210,979 - slt Synpcc7942_2131 0.87 -1.0 2,210,984 - slt Synpcc7942_2131 0.87 -3.1 2,210,984 - slt Synpcc7942_2131 0.87 -0.7 2,210,989 + slt Synpcc7942_2131 0.87 -2.3 2,211,013 + slt Synpcc7942_2131 0.88 -0.6 2,211,031 + slt Synpcc7942_2131 0.89 -1.2 2,211,055 + -2.4 2,211,055 + -1.2 2,211,065 + -2.0 2,211,096 + -3.0 2,211,096 + -1.6 2,211,263 + -3.5 2,211,263 + -1.8 2,211,271 - -2.0 2,211,304 - -3.1 2,211,307 - -0.6 2,211,310 + +1.1 2,211,374 - -2.6 2,211,395 - -2.5 2,211,395 - -0.7 2,211,422 + -2.3 2,211,422 + +2.3 2,211,423 - -3.8 2,211,423 - +0.3 2,211,442 + -3.9 2,211,450 - -3.1 2,211,453 - -3.7 2,211,465 + -3.5 2,211,495 - -0.6 2,211,534 + -3.3 2,211,540 - -0.7 2,211,540 - -4.3 2,211,542 - -1.5 2,211,547 - -1.4 2,211,591 + -1.2 2,211,599 - -1.0 2,211,626 + -1.6 2,211,730 - manB Synpcc7942_2132 0.13 -1.6 2,211,730 - manB Synpcc7942_2132 0.13 -2.9 2,211,799 + manB Synpcc7942_2132 0.18 -1.8 2,211,891 - manB Synpcc7942_2132 0.24 -3.0 2,212,053 + manB Synpcc7942_2132 0.36 -2.8 2,212,204 + manB Synpcc7942_2132 0.46 -2.8 2,212,370 + manB Synpcc7942_2132 0.58 -1.2 2,212,605 - manB Synpcc7942_2132 0.74 -2.5 2,212,794 - manB Synpcc7942_2132 0.88 -1.7 2,212,799 - manB Synpcc7942_2132 0.88 -1.6 2,212,964 - -2.3 2,212,968 + +3.1 2,212,969 - +1.1 2,212,969 - +2.2 2,212,997 + +0.7 2,213,007 + +0.7 2,213,013 + -0.3 2,213,015 - -1.3 2,213,057 + Synpcc7942_2133 0.17 -0.6 2,213,060 - Synpcc7942_2133 0.18 -3.3 2,213,063 + Synpcc7942_2133 0.18 -1.7 2,213,071 - Synpcc7942_2133 0.20 +1.1 2,213,093 + Synpcc7942_2133 0.24 -1.0 2,213,175 + Synpcc7942_2133 0.40 -0.6 2,213,175 + Synpcc7942_2133 0.40 -1.9 2,213,183 - Synpcc7942_2133 0.42 -1.8 2,213,195 - Synpcc7942_2133 0.44 -0.2 2,213,203 - Synpcc7942_2133 0.46 -1.2 2,213,203 - Synpcc7942_2133 0.46 -1.2 2,213,207 - Synpcc7942_2133 0.47 -1.9 2,213,246 + Synpcc7942_2133 0.54 -2.2 2,213,259 - Synpcc7942_2133 0.57 -0.1 2,213,269 - Synpcc7942_2133 0.59 -1.9 2,213,272 - Synpcc7942_2133 0.60 -2.5 2,213,276 - Synpcc7942_2133 0.60 -2.1 2,213,281 - Synpcc7942_2133 0.61 -2.5 2,213,375 + Synpcc7942_2133 0.80 -2.1 2,213,375 + Synpcc7942_2133 0.80 -1.2 2,213,375 + Synpcc7942_2133 0.80 -1.7 2,213,375 + Synpcc7942_2133 0.80 -2.6 2,213,375 + Synpcc7942_2133 0.80 -1.4 2,213,380 + Synpcc7942_2133 0.81 -1.2 2,213,383 - Synpcc7942_2133 0.81 -1.4 2,213,383 - Synpcc7942_2133 0.81 -1.2 2,213,383 - Synpcc7942_2133 0.81 +1.3 2,213,384 + Synpcc7942_2133 0.82 -0.5 2,213,385 + Synpcc7942_2133 0.82 -1.3 2,213,385 + Synpcc7942_2133 0.82 -0.7 2,213,393 - Synpcc7942_2133 0.83 -1.2 2,213,393 - Synpcc7942_2133 0.83 +0.0 2,213,393 - Synpcc7942_2133 0.83 -2.0 2,213,405 + Synpcc7942_2133 0.86 -0.4 2,213,423 - Synpcc7942_2133 0.89 -0.2 2,213,425 + Synpcc7942_2133 0.90 -2.0 2,213,437 - -1.8 2,213,444 - -1.8 2,213,507 - +3.0 2,213,507 - +0.9 2,213,525 - +0.4 2,213,545 + +0.2 2,213,590 + +0.3 2,213,590 - -1.5 2,213,598 - -1.0 2,213,605 - +4.8 2,213,624 - -1.3 2,213,624 - -0.8 2,213,639 + -0.8 2,213,639 + +2.0 2,213,647 - -0.8 2,213,647 - +3.7 2,213,647 - -0.3 2,213,650 + +1.1 2,213,652 - +0.5 2,213,682 - -3.3 2,213,709 - +0.2 2,213,752 - +0.5 2,213,753 - +0.6 2,213,787 - amiA Synpcc7942_2134 0.12 -0.5 2,213,842 - amiA Synpcc7942_2134 0.17 -2.6 2,213,854 + amiA Synpcc7942_2134 0.19 +0.4 2,213,854 + amiA Synpcc7942_2134 0.19 +0.6 2,213,862 - amiA Synpcc7942_2134 0.19 -2.2 2,213,877 - amiA Synpcc7942_2134 0.21 -2.3 2,213,877 - amiA Synpcc7942_2134 0.21 -2.6 2,213,877 - amiA Synpcc7942_2134 0.21 -0.8 2,213,877 - amiA Synpcc7942_2134 0.21 -0.7 2,213,897 - amiA Synpcc7942_2134 0.23 -0.1 2,213,918 + amiA Synpcc7942_2134 0.25 -0.9 2,213,922 - amiA Synpcc7942_2134 0.25 -1.3 2,213,929 + amiA Synpcc7942_2134 0.26 -1.8 2,213,934 + amiA Synpcc7942_2134 0.26 +1.2 2,213,953 - amiA Synpcc7942_2134 0.28 +0.0
Or see this region's nucleotide sequence