Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2129 and Synpcc7942_2130 are separated by 7 nucleotides Synpcc7942_2130 and slt are separated by 32 nucleotides slt and manB are separated by 293 nucleotides
Synpcc7942_2129: Synpcc7942_2129 - iron-sulfur cluster binding protein, at 2,208,154 to 2,208,558
_2129
Synpcc7942_2130: Synpcc7942_2130 - hypothetical protein, at 2,208,566 to 2,209,150
_2130
Synpcc7942_2131: slt - probable soluble lytic transglycosylase, at 2,209,183 to 2,211,255
slt
Synpcc7942_2132: manB - phosphoglucosamine mutase, at 2,211,549 to 2,212,967
manB
Position (kb)
2209
2210
2211
2212 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 2208.274 kb on - strand, within Synpcc7942_2129 at 2208.353 kb on + strand, within Synpcc7942_2129 at 2208.361 kb on - strand, within Synpcc7942_2129 at 2208.422 kb on + strand, within Synpcc7942_2129 at 2208.422 kb on + strand, within Synpcc7942_2129 at 2208.422 kb on + strand, within Synpcc7942_2129 at 2208.430 kb on - strand, within Synpcc7942_2129 at 2208.430 kb on - strand, within Synpcc7942_2129 at 2208.500 kb on - strand, within Synpcc7942_2129 at 2208.502 kb on + strand, within Synpcc7942_2129 at 2208.508 kb on + strand, within Synpcc7942_2129 at 2208.521 kb on - strand at 2208.525 kb on - strand at 2208.527 kb on + strand at 2208.906 kb on - strand, within Synpcc7942_2130 at 2208.960 kb on + strand, within Synpcc7942_2130 at 2208.989 kb on + strand, within Synpcc7942_2130 at 2209.010 kb on - strand, within Synpcc7942_2130 at 2209.016 kb on - strand, within Synpcc7942_2130 at 2209.030 kb on - strand, within Synpcc7942_2130 at 2209.037 kb on + strand, within Synpcc7942_2130 at 2209.050 kb on - strand, within Synpcc7942_2130 at 2209.075 kb on + strand, within Synpcc7942_2130 at 2209.117 kb on + strand at 2209.157 kb on + strand at 2209.189 kb on + strand at 2209.189 kb on + strand at 2209.217 kb on - strand at 2209.276 kb on - strand at 2209.326 kb on + strand at 2209.392 kb on - strand, within slt at 2209.461 kb on - strand, within slt at 2209.525 kb on + strand, within slt at 2209.562 kb on + strand, within slt at 2209.591 kb on + strand, within slt at 2209.598 kb on + strand, within slt at 2209.640 kb on - strand, within slt at 2209.664 kb on + strand, within slt at 2209.672 kb on - strand, within slt at 2209.724 kb on + strand, within slt at 2209.724 kb on + strand, within slt at 2209.724 kb on + strand, within slt at 2209.724 kb on + strand, within slt at 2209.724 kb on + strand, within slt at 2209.732 kb on - strand, within slt at 2209.732 kb on - strand, within slt at 2209.760 kb on + strand, within slt at 2209.776 kb on - strand, within slt at 2209.817 kb on + strand, within slt at 2209.818 kb on + strand, within slt at 2209.832 kb on - strand, within slt at 2209.855 kb on - strand, within slt at 2209.864 kb on + strand, within slt at 2209.865 kb on + strand, within slt at 2209.873 kb on - strand, within slt at 2209.884 kb on - strand, within slt at 2209.894 kb on - strand, within slt at 2209.903 kb on + strand, within slt at 2209.950 kb on - strand, within slt at 2209.970 kb on + strand, within slt at 2209.975 kb on + strand, within slt at 2209.975 kb on + strand, within slt at 2210.025 kb on + strand, within slt at 2210.098 kb on - strand, within slt at 2210.098 kb on - strand, within slt at 2210.140 kb on + strand, within slt at 2210.163 kb on - strand, within slt at 2210.209 kb on + strand, within slt at 2210.209 kb on + strand, within slt at 2210.290 kb on + strand, within slt at 2210.290 kb on + strand, within slt at 2210.298 kb on - strand, within slt at 2210.298 kb on - strand, within slt at 2210.298 kb on - strand, within slt at 2210.298 kb on - strand, within slt at 2210.298 kb on - strand, within slt at 2210.298 kb on - strand, within slt at 2210.334 kb on + strand, within slt at 2210.334 kb on - strand, within slt at 2210.361 kb on + strand, within slt at 2210.362 kb on + strand, within slt at 2210.362 kb on + strand, within slt at 2210.376 kb on - strand, within slt at 2210.388 kb on - strand, within slt at 2210.389 kb on - strand, within slt at 2210.404 kb on + strand, within slt at 2210.439 kb on - strand, within slt at 2210.441 kb on - strand, within slt at 2210.450 kb on + strand, within slt at 2210.464 kb on + strand, within slt at 2210.469 kb on + strand, within slt at 2210.469 kb on + strand, within slt at 2210.469 kb on + strand, within slt at 2210.472 kb on - strand, within slt at 2210.477 kb on - strand, within slt at 2210.496 kb on + strand, within slt at 2210.504 kb on - strand, within slt at 2210.514 kb on - strand, within slt at 2210.522 kb on + strand, within slt at 2210.556 kb on - strand, within slt at 2210.559 kb on + strand, within slt at 2210.583 kb on + strand, within slt at 2210.600 kb on + strand, within slt at 2210.620 kb on - strand, within slt at 2210.639 kb on + strand, within slt at 2210.727 kb on + strand, within slt at 2210.735 kb on - strand, within slt at 2210.787 kb on + strand, within slt at 2210.787 kb on + strand, within slt at 2210.797 kb on - strand, within slt at 2210.804 kb on + strand, within slt at 2210.812 kb on - strand, within slt at 2210.814 kb on + strand, within slt at 2210.814 kb on + strand, within slt at 2210.817 kb on - strand, within slt at 2210.834 kb on + strand, within slt at 2210.834 kb on + strand, within slt at 2210.834 kb on + strand, within slt at 2210.834 kb on + strand, within slt at 2210.842 kb on - strand, within slt at 2210.848 kb on + strand, within slt at 2210.872 kb on + strand, within slt at 2210.899 kb on + strand, within slt at 2210.899 kb on + strand, within slt at 2210.899 kb on + strand, within slt at 2210.909 kb on - strand, within slt at 2210.910 kb on + strand, within slt at 2210.915 kb on - strand, within slt at 2210.923 kb on - strand, within slt at 2210.927 kb on + strand, within slt at 2210.932 kb on + strand, within slt at 2210.933 kb on - strand, within slt at 2210.937 kb on + strand, within slt at 2210.937 kb on + strand, within slt at 2210.945 kb on - strand, within slt at 2210.945 kb on - strand, within slt at 2210.945 kb on - strand, within slt at 2210.955 kb on - strand, within slt at 2210.966 kb on + strand, within slt at 2210.971 kb on + strand, within slt at 2210.976 kb on + strand, within slt at 2210.976 kb on + strand, within slt at 2210.976 kb on + strand, within slt at 2210.979 kb on - strand, within slt at 2210.984 kb on - strand, within slt at 2210.984 kb on - strand, within slt at 2210.989 kb on + strand, within slt at 2211.013 kb on + strand, within slt at 2211.031 kb on + strand, within slt at 2211.055 kb on + strand at 2211.055 kb on + strand at 2211.065 kb on + strand at 2211.096 kb on + strand at 2211.096 kb on + strand at 2211.263 kb on + strand at 2211.263 kb on + strand at 2211.271 kb on - strand at 2211.304 kb on - strand at 2211.307 kb on - strand at 2211.310 kb on + strand at 2211.374 kb on - strand at 2211.395 kb on - strand at 2211.395 kb on - strand at 2211.422 kb on + strand at 2211.422 kb on + strand at 2211.423 kb on - strand at 2211.423 kb on - strand at 2211.442 kb on + strand at 2211.450 kb on - strand at 2211.453 kb on - strand at 2211.465 kb on + strand at 2211.495 kb on - strand at 2211.534 kb on + strand at 2211.540 kb on - strand at 2211.540 kb on - strand at 2211.542 kb on - strand at 2211.547 kb on - strand at 2211.591 kb on + strand at 2211.599 kb on - strand at 2211.626 kb on + strand at 2211.730 kb on - strand, within manB at 2211.730 kb on - strand, within manB at 2211.799 kb on + strand, within manB at 2211.891 kb on - strand, within manB at 2212.053 kb on + strand, within manB at 2212.204 kb on + strand, within manB
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,208,274 - Synpcc7942_2129 0.30 -1.0 2,208,353 + Synpcc7942_2129 0.49 -1.9 2,208,361 - Synpcc7942_2129 0.51 -2.1 2,208,422 + Synpcc7942_2129 0.66 -2.7 2,208,422 + Synpcc7942_2129 0.66 -1.2 2,208,422 + Synpcc7942_2129 0.66 -3.6 2,208,430 - Synpcc7942_2129 0.68 -1.8 2,208,430 - Synpcc7942_2129 0.68 -1.4 2,208,500 - Synpcc7942_2129 0.85 -2.6 2,208,502 + Synpcc7942_2129 0.86 -4.6 2,208,508 + Synpcc7942_2129 0.87 -2.7 2,208,521 - -2.9 2,208,525 - -2.8 2,208,527 + -2.3 2,208,906 - Synpcc7942_2130 0.58 -1.8 2,208,960 + Synpcc7942_2130 0.67 -0.9 2,208,989 + Synpcc7942_2130 0.72 +0.3 2,209,010 - Synpcc7942_2130 0.76 +0.4 2,209,016 - Synpcc7942_2130 0.77 -3.7 2,209,030 - Synpcc7942_2130 0.79 -2.5 2,209,037 + Synpcc7942_2130 0.81 +0.4 2,209,050 - Synpcc7942_2130 0.83 -1.0 2,209,075 + Synpcc7942_2130 0.87 -1.0 2,209,117 + +2.6 2,209,157 + -1.3 2,209,189 + +2.9 2,209,189 + +2.0 2,209,217 - +4.6 2,209,276 - +0.0 2,209,326 + -3.8 2,209,392 - slt Synpcc7942_2131 0.10 +0.0 2,209,461 - slt Synpcc7942_2131 0.13 -2.6 2,209,525 + slt Synpcc7942_2131 0.16 -3.4 2,209,562 + slt Synpcc7942_2131 0.18 +0.0 2,209,591 + slt Synpcc7942_2131 0.20 -3.0 2,209,598 + slt Synpcc7942_2131 0.20 -0.6 2,209,640 - slt Synpcc7942_2131 0.22 -2.7 2,209,664 + slt Synpcc7942_2131 0.23 -1.4 2,209,672 - slt Synpcc7942_2131 0.24 -3.2 2,209,724 + slt Synpcc7942_2131 0.26 -5.5 2,209,724 + slt Synpcc7942_2131 0.26 +1.0 2,209,724 + slt Synpcc7942_2131 0.26 -0.8 2,209,724 + slt Synpcc7942_2131 0.26 -4.9 2,209,724 + slt Synpcc7942_2131 0.26 +0.5 2,209,732 - slt Synpcc7942_2131 0.26 +1.0 2,209,732 - slt Synpcc7942_2131 0.26 -2.0 2,209,760 + slt Synpcc7942_2131 0.28 -3.3 2,209,776 - slt Synpcc7942_2131 0.29 -2.3 2,209,817 + slt Synpcc7942_2131 0.31 -2.9 2,209,818 + slt Synpcc7942_2131 0.31 -1.9 2,209,832 - slt Synpcc7942_2131 0.31 -3.2 2,209,855 - slt Synpcc7942_2131 0.32 -2.4 2,209,864 + slt Synpcc7942_2131 0.33 -0.3 2,209,865 + slt Synpcc7942_2131 0.33 -1.8 2,209,873 - slt Synpcc7942_2131 0.33 -5.0 2,209,884 - slt Synpcc7942_2131 0.34 -4.1 2,209,894 - slt Synpcc7942_2131 0.34 -2.1 2,209,903 + slt Synpcc7942_2131 0.35 -1.9 2,209,950 - slt Synpcc7942_2131 0.37 -3.3 2,209,970 + slt Synpcc7942_2131 0.38 -3.5 2,209,975 + slt Synpcc7942_2131 0.38 -5.7 2,209,975 + slt Synpcc7942_2131 0.38 -3.0 2,210,025 + slt Synpcc7942_2131 0.41 -2.5 2,210,098 - slt Synpcc7942_2131 0.44 -4.3 2,210,098 - slt Synpcc7942_2131 0.44 -0.6 2,210,140 + slt Synpcc7942_2131 0.46 -0.8 2,210,163 - slt Synpcc7942_2131 0.47 -1.7 2,210,209 + slt Synpcc7942_2131 0.49 -0.8 2,210,209 + slt Synpcc7942_2131 0.49 -0.3 2,210,290 + slt Synpcc7942_2131 0.53 -0.6 2,210,290 + slt Synpcc7942_2131 0.53 +0.0 2,210,298 - slt Synpcc7942_2131 0.54 -1.1 2,210,298 - slt Synpcc7942_2131 0.54 -3.6 2,210,298 - slt Synpcc7942_2131 0.54 -1.3 2,210,298 - slt Synpcc7942_2131 0.54 -2.1 2,210,298 - slt Synpcc7942_2131 0.54 -6.3 2,210,298 - slt Synpcc7942_2131 0.54 -1.7 2,210,334 + slt Synpcc7942_2131 0.56 -1.0 2,210,334 - slt Synpcc7942_2131 0.56 -1.8 2,210,361 + slt Synpcc7942_2131 0.57 -2.6 2,210,362 + slt Synpcc7942_2131 0.57 -3.4 2,210,362 + slt Synpcc7942_2131 0.57 -4.3 2,210,376 - slt Synpcc7942_2131 0.58 -2.8 2,210,388 - slt Synpcc7942_2131 0.58 -1.7 2,210,389 - slt Synpcc7942_2131 0.58 -3.5 2,210,404 + slt Synpcc7942_2131 0.59 -1.2 2,210,439 - slt Synpcc7942_2131 0.61 -0.8 2,210,441 - slt Synpcc7942_2131 0.61 -1.6 2,210,450 + slt Synpcc7942_2131 0.61 -0.7 2,210,464 + slt Synpcc7942_2131 0.62 -6.1 2,210,469 + slt Synpcc7942_2131 0.62 -2.2 2,210,469 + slt Synpcc7942_2131 0.62 -2.8 2,210,469 + slt Synpcc7942_2131 0.62 -3.0 2,210,472 - slt Synpcc7942_2131 0.62 -1.3 2,210,477 - slt Synpcc7942_2131 0.62 -3.6 2,210,496 + slt Synpcc7942_2131 0.63 -3.4 2,210,504 - slt Synpcc7942_2131 0.64 -2.6 2,210,514 - slt Synpcc7942_2131 0.64 -1.3 2,210,522 + slt Synpcc7942_2131 0.65 -1.4 2,210,556 - slt Synpcc7942_2131 0.66 -3.7 2,210,559 + slt Synpcc7942_2131 0.66 -1.2 2,210,583 + slt Synpcc7942_2131 0.68 -3.7 2,210,600 + slt Synpcc7942_2131 0.68 -4.6 2,210,620 - slt Synpcc7942_2131 0.69 -2.6 2,210,639 + slt Synpcc7942_2131 0.70 -1.8 2,210,727 + slt Synpcc7942_2131 0.74 -1.4 2,210,735 - slt Synpcc7942_2131 0.75 -3.0 2,210,787 + slt Synpcc7942_2131 0.77 -2.6 2,210,787 + slt Synpcc7942_2131 0.77 -1.3 2,210,797 - slt Synpcc7942_2131 0.78 -1.4 2,210,804 + slt Synpcc7942_2131 0.78 -0.6 2,210,812 - slt Synpcc7942_2131 0.79 -3.0 2,210,814 + slt Synpcc7942_2131 0.79 -3.9 2,210,814 + slt Synpcc7942_2131 0.79 -4.7 2,210,817 - slt Synpcc7942_2131 0.79 -2.5 2,210,834 + slt Synpcc7942_2131 0.80 -4.0 2,210,834 + slt Synpcc7942_2131 0.80 -2.3 2,210,834 + slt Synpcc7942_2131 0.80 -5.1 2,210,834 + slt Synpcc7942_2131 0.80 -0.8 2,210,842 - slt Synpcc7942_2131 0.80 -1.0 2,210,848 + slt Synpcc7942_2131 0.80 -2.8 2,210,872 + slt Synpcc7942_2131 0.81 -3.9 2,210,899 + slt Synpcc7942_2131 0.83 -1.7 2,210,899 + slt Synpcc7942_2131 0.83 -2.7 2,210,899 + slt Synpcc7942_2131 0.83 -3.2 2,210,909 - slt Synpcc7942_2131 0.83 -2.6 2,210,910 + slt Synpcc7942_2131 0.83 -2.7 2,210,915 - slt Synpcc7942_2131 0.84 +0.0 2,210,923 - slt Synpcc7942_2131 0.84 -3.1 2,210,927 + slt Synpcc7942_2131 0.84 -0.6 2,210,932 + slt Synpcc7942_2131 0.84 -4.4 2,210,933 - slt Synpcc7942_2131 0.84 -1.0 2,210,937 + slt Synpcc7942_2131 0.85 -3.8 2,210,937 + slt Synpcc7942_2131 0.85 -2.4 2,210,945 - slt Synpcc7942_2131 0.85 -2.3 2,210,945 - slt Synpcc7942_2131 0.85 -2.2 2,210,945 - slt Synpcc7942_2131 0.85 -1.0 2,210,955 - slt Synpcc7942_2131 0.85 -3.2 2,210,966 + slt Synpcc7942_2131 0.86 -2.4 2,210,971 + slt Synpcc7942_2131 0.86 -5.9 2,210,976 + slt Synpcc7942_2131 0.86 -1.0 2,210,976 + slt Synpcc7942_2131 0.86 -3.9 2,210,976 + slt Synpcc7942_2131 0.86 -1.8 2,210,979 - slt Synpcc7942_2131 0.87 -1.0 2,210,984 - slt Synpcc7942_2131 0.87 -3.1 2,210,984 - slt Synpcc7942_2131 0.87 -0.7 2,210,989 + slt Synpcc7942_2131 0.87 -2.3 2,211,013 + slt Synpcc7942_2131 0.88 -0.6 2,211,031 + slt Synpcc7942_2131 0.89 -1.2 2,211,055 + -2.4 2,211,055 + -1.2 2,211,065 + -2.0 2,211,096 + -3.0 2,211,096 + -1.6 2,211,263 + -3.5 2,211,263 + -1.8 2,211,271 - -2.0 2,211,304 - -3.1 2,211,307 - -0.6 2,211,310 + +1.1 2,211,374 - -2.6 2,211,395 - -2.5 2,211,395 - -0.7 2,211,422 + -2.3 2,211,422 + +2.3 2,211,423 - -3.8 2,211,423 - +0.3 2,211,442 + -3.9 2,211,450 - -3.1 2,211,453 - -3.7 2,211,465 + -3.5 2,211,495 - -0.6 2,211,534 + -3.3 2,211,540 - -0.7 2,211,540 - -4.3 2,211,542 - -1.5 2,211,547 - -1.4 2,211,591 + -1.2 2,211,599 - -1.0 2,211,626 + -1.6 2,211,730 - manB Synpcc7942_2132 0.13 -1.6 2,211,730 - manB Synpcc7942_2132 0.13 -2.9 2,211,799 + manB Synpcc7942_2132 0.18 -1.8 2,211,891 - manB Synpcc7942_2132 0.24 -3.0 2,212,053 + manB Synpcc7942_2132 0.36 -2.8 2,212,204 + manB Synpcc7942_2132 0.46 -2.8
Or see this region's nucleotide sequence