Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nblA and txlA are separated by 159 nucleotides txlA and Synpcc7942_2129 are separated by 72 nucleotides Synpcc7942_2129 and Synpcc7942_2130 are separated by 7 nucleotides Synpcc7942_2130 and slt are separated by 32 nucleotides
Synpcc7942_2127: nblA - phycobilisome degradation protein NblA, at 2,207,167 to 2,207,346
nblA
Synpcc7942_2128: txlA - thioredoxin, at 2,207,506 to 2,208,081
txlA
Synpcc7942_2129: Synpcc7942_2129 - iron-sulfur cluster binding protein, at 2,208,154 to 2,208,558
_2129
Synpcc7942_2130: Synpcc7942_2130 - hypothetical protein, at 2,208,566 to 2,209,150
_2130
Synpcc7942_2131: slt - probable soluble lytic transglycosylase, at 2,209,183 to 2,211,255
slt
Position (kb)
2208
2209 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2207.164 kb on + strand at 2207.198 kb on + strand, within nblA at 2207.206 kb on - strand, within nblA at 2207.228 kb on - strand, within nblA at 2207.231 kb on + strand, within nblA at 2207.239 kb on + strand, within nblA at 2207.305 kb on + strand, within nblA at 2207.305 kb on + strand, within nblA at 2207.323 kb on - strand, within nblA at 2207.340 kb on + strand at 2207.342 kb on + strand at 2207.348 kb on - strand at 2207.350 kb on - strand at 2207.352 kb on + strand at 2207.360 kb on - strand at 2207.360 kb on - strand at 2207.380 kb on - strand at 2207.420 kb on - strand at 2207.431 kb on - strand at 2207.443 kb on + strand at 2207.491 kb on + strand at 2207.494 kb on - strand at 2207.499 kb on - strand at 2207.511 kb on - strand at 2207.521 kb on + strand at 2207.626 kb on - strand, within txlA at 2207.626 kb on - strand, within txlA at 2207.626 kb on - strand, within txlA at 2208.049 kb on - strand at 2208.085 kb on + strand at 2208.086 kb on + strand at 2208.086 kb on + strand at 2208.086 kb on + strand at 2208.094 kb on - strand at 2208.094 kb on - strand at 2208.096 kb on + strand at 2208.096 kb on + strand at 2208.096 kb on + strand at 2208.104 kb on - strand at 2208.104 kb on - strand at 2208.120 kb on + strand at 2208.130 kb on + strand at 2208.130 kb on + strand at 2208.130 kb on - strand at 2208.137 kb on - strand at 2208.138 kb on - strand at 2208.151 kb on + strand at 2208.274 kb on - strand, within Synpcc7942_2129 at 2208.353 kb on + strand, within Synpcc7942_2129 at 2208.361 kb on - strand, within Synpcc7942_2129 at 2208.422 kb on + strand, within Synpcc7942_2129 at 2208.422 kb on + strand, within Synpcc7942_2129 at 2208.422 kb on + strand, within Synpcc7942_2129 at 2208.430 kb on - strand, within Synpcc7942_2129 at 2208.430 kb on - strand, within Synpcc7942_2129 at 2208.500 kb on - strand, within Synpcc7942_2129 at 2208.502 kb on + strand, within Synpcc7942_2129 at 2208.508 kb on + strand, within Synpcc7942_2129 at 2208.521 kb on - strand at 2208.525 kb on - strand at 2208.527 kb on + strand at 2208.906 kb on - strand, within Synpcc7942_2130 at 2208.960 kb on + strand, within Synpcc7942_2130 at 2208.989 kb on + strand, within Synpcc7942_2130 at 2209.010 kb on - strand, within Synpcc7942_2130 at 2209.016 kb on - strand, within Synpcc7942_2130 at 2209.030 kb on - strand, within Synpcc7942_2130 at 2209.037 kb on + strand, within Synpcc7942_2130 at 2209.050 kb on - strand, within Synpcc7942_2130 at 2209.075 kb on + strand, within Synpcc7942_2130 at 2209.117 kb on + strand at 2209.157 kb on + strand at 2209.189 kb on + strand at 2209.189 kb on + strand at 2209.217 kb on - strand at 2209.276 kb on - strand at 2209.326 kb on + strand at 2209.392 kb on - strand, within slt at 2209.461 kb on - strand, within slt at 2209.525 kb on + strand, within slt
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,207,164 + +0.2 2,207,198 + nblA Synpcc7942_2127 0.17 -1.6 2,207,206 - nblA Synpcc7942_2127 0.22 +0.3 2,207,228 - nblA Synpcc7942_2127 0.34 +3.0 2,207,231 + nblA Synpcc7942_2127 0.36 +0.6 2,207,239 + nblA Synpcc7942_2127 0.40 -2.0 2,207,305 + nblA Synpcc7942_2127 0.77 -0.6 2,207,305 + nblA Synpcc7942_2127 0.77 +0.7 2,207,323 - nblA Synpcc7942_2127 0.87 -2.0 2,207,340 + +0.3 2,207,342 + +1.2 2,207,348 - +1.7 2,207,350 - +1.1 2,207,352 + +0.1 2,207,360 - -1.6 2,207,360 - +3.0 2,207,380 - -0.9 2,207,420 - -1.5 2,207,431 - -0.9 2,207,443 + -0.8 2,207,491 + -0.6 2,207,494 - -2.7 2,207,499 - +2.9 2,207,511 - -0.3 2,207,521 + +0.9 2,207,626 - txlA Synpcc7942_2128 0.21 -0.1 2,207,626 - txlA Synpcc7942_2128 0.21 +5.2 2,207,626 - txlA Synpcc7942_2128 0.21 +4.6 2,208,049 - +2.3 2,208,085 + -0.8 2,208,086 + +1.6 2,208,086 + +0.2 2,208,086 + +1.9 2,208,094 - +5.6 2,208,094 - +5.2 2,208,096 + +0.6 2,208,096 + -2.3 2,208,096 + +3.4 2,208,104 - +4.4 2,208,104 - +1.4 2,208,120 + +0.2 2,208,130 + +0.3 2,208,130 + +0.5 2,208,130 - +1.1 2,208,137 - -1.8 2,208,138 - +1.1 2,208,151 + +2.2 2,208,274 - Synpcc7942_2129 0.30 -1.0 2,208,353 + Synpcc7942_2129 0.49 -1.9 2,208,361 - Synpcc7942_2129 0.51 -2.1 2,208,422 + Synpcc7942_2129 0.66 -2.7 2,208,422 + Synpcc7942_2129 0.66 -1.2 2,208,422 + Synpcc7942_2129 0.66 -3.6 2,208,430 - Synpcc7942_2129 0.68 -1.8 2,208,430 - Synpcc7942_2129 0.68 -1.4 2,208,500 - Synpcc7942_2129 0.85 -2.6 2,208,502 + Synpcc7942_2129 0.86 -4.6 2,208,508 + Synpcc7942_2129 0.87 -2.7 2,208,521 - -2.9 2,208,525 - -2.8 2,208,527 + -2.3 2,208,906 - Synpcc7942_2130 0.58 -1.8 2,208,960 + Synpcc7942_2130 0.67 -0.9 2,208,989 + Synpcc7942_2130 0.72 +0.3 2,209,010 - Synpcc7942_2130 0.76 +0.4 2,209,016 - Synpcc7942_2130 0.77 -3.7 2,209,030 - Synpcc7942_2130 0.79 -2.5 2,209,037 + Synpcc7942_2130 0.81 +0.4 2,209,050 - Synpcc7942_2130 0.83 -1.0 2,209,075 + Synpcc7942_2130 0.87 -1.0 2,209,117 + +2.6 2,209,157 + -1.3 2,209,189 + +2.9 2,209,189 + +2.0 2,209,217 - +4.6 2,209,276 - +0.0 2,209,326 + -3.8 2,209,392 - slt Synpcc7942_2131 0.10 +0.0 2,209,461 - slt Synpcc7942_2131 0.13 -2.6 2,209,525 + slt Synpcc7942_2131 0.16 -3.4
Or see this region's nucleotide sequence