Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bcp and Synpcc7942_2125 are separated by 122 nucleotides Synpcc7942_2125 and Synpcc7942_2126 overlap by 4 nucleotides Synpcc7942_2126 and nblA are separated by 362 nucleotides nblA and txlA are separated by 159 nucleotides
Synpcc7942_2124: bcp - acetylpolyamine aminohydolase, at 2,204,908 to 2,205,879
bcp
Synpcc7942_2125: Synpcc7942_2125 - hypothetical protein, at 2,206,002 to 2,206,403
_2125
Synpcc7942_2126: Synpcc7942_2126 - hypothetical protein, at 2,206,400 to 2,206,804
_2126
Synpcc7942_2127: nblA - phycobilisome degradation protein NblA, at 2,207,167 to 2,207,346
nblA
Synpcc7942_2128: txlA - thioredoxin, at 2,207,506 to 2,208,081
txlA
Position (kb)
2206
2207 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2205.482 kb on - strand, within bcp at 2205.500 kb on - strand, within bcp at 2205.601 kb on - strand, within bcp at 2205.732 kb on - strand, within bcp at 2205.732 kb on - strand, within bcp at 2205.745 kb on - strand, within bcp at 2205.865 kb on - strand at 2205.874 kb on + strand at 2205.874 kb on + strand at 2205.881 kb on + strand at 2205.905 kb on + strand at 2205.905 kb on + strand at 2205.913 kb on - strand at 2205.925 kb on + strand at 2205.925 kb on + strand at 2205.930 kb on + strand at 2205.935 kb on + strand at 2205.935 kb on + strand at 2205.938 kb on - strand at 2205.940 kb on + strand at 2205.943 kb on - strand at 2205.943 kb on - strand at 2205.950 kb on - strand at 2205.961 kb on + strand at 2205.962 kb on + strand at 2205.962 kb on + strand at 2205.962 kb on + strand at 2205.970 kb on - strand at 2205.970 kb on - strand at 2205.970 kb on - strand at 2205.970 kb on - strand at 2205.985 kb on + strand at 2205.987 kb on + strand at 2206.005 kb on - strand at 2206.005 kb on - strand at 2206.017 kb on - strand at 2206.034 kb on - strand at 2206.038 kb on + strand at 2206.046 kb on + strand, within Synpcc7942_2125 at 2206.065 kb on - strand, within Synpcc7942_2125 at 2206.112 kb on + strand, within Synpcc7942_2125 at 2206.114 kb on + strand, within Synpcc7942_2125 at 2206.127 kb on - strand at 2206.132 kb on - strand, within Synpcc7942_2125 at 2206.132 kb on - strand, within Synpcc7942_2125 at 2206.151 kb on - strand, within Synpcc7942_2125 at 2206.155 kb on - strand, within Synpcc7942_2125 at 2206.185 kb on - strand, within Synpcc7942_2125 at 2206.192 kb on + strand, within Synpcc7942_2125 at 2206.200 kb on - strand, within Synpcc7942_2125 at 2206.200 kb on - strand, within Synpcc7942_2125 at 2206.210 kb on - strand, within Synpcc7942_2125 at 2206.245 kb on + strand, within Synpcc7942_2125 at 2206.245 kb on + strand, within Synpcc7942_2125 at 2206.253 kb on - strand, within Synpcc7942_2125 at 2206.267 kb on + strand, within Synpcc7942_2125 at 2206.267 kb on + strand, within Synpcc7942_2125 at 2206.267 kb on + strand, within Synpcc7942_2125 at 2206.290 kb on + strand at 2206.291 kb on + strand, within Synpcc7942_2125 at 2206.316 kb on - strand, within Synpcc7942_2125 at 2206.373 kb on - strand at 2206.395 kb on - strand at 2206.398 kb on + strand at 2206.431 kb on - strand at 2206.433 kb on + strand at 2206.433 kb on + strand at 2206.433 kb on + strand at 2206.433 kb on + strand at 2206.441 kb on - strand, within Synpcc7942_2126 at 2206.441 kb on - strand, within Synpcc7942_2126 at 2206.441 kb on - strand, within Synpcc7942_2126 at 2206.441 kb on - strand, within Synpcc7942_2126 at 2206.452 kb on - strand, within Synpcc7942_2126 at 2206.456 kb on + strand, within Synpcc7942_2126 at 2206.464 kb on - strand, within Synpcc7942_2126 at 2206.471 kb on - strand, within Synpcc7942_2126 at 2206.519 kb on - strand, within Synpcc7942_2126 at 2206.519 kb on - strand, within Synpcc7942_2126 at 2206.524 kb on + strand, within Synpcc7942_2126 at 2206.532 kb on - strand, within Synpcc7942_2126 at 2206.555 kb on + strand, within Synpcc7942_2126 at 2206.589 kb on - strand, within Synpcc7942_2126 at 2206.600 kb on - strand, within Synpcc7942_2126 at 2206.614 kb on - strand, within Synpcc7942_2126 at 2206.683 kb on + strand, within Synpcc7942_2126 at 2206.683 kb on + strand, within Synpcc7942_2126 at 2206.683 kb on + strand, within Synpcc7942_2126 at 2206.691 kb on - strand, within Synpcc7942_2126 at 2206.691 kb on - strand, within Synpcc7942_2126 at 2206.691 kb on - strand, within Synpcc7942_2126 at 2206.693 kb on + strand, within Synpcc7942_2126 at 2206.700 kb on - strand, within Synpcc7942_2126 at 2206.717 kb on - strand, within Synpcc7942_2126 at 2206.728 kb on - strand, within Synpcc7942_2126 at 2206.728 kb on - strand, within Synpcc7942_2126 at 2206.734 kb on + strand, within Synpcc7942_2126 at 2206.742 kb on - strand, within Synpcc7942_2126 at 2206.742 kb on - strand, within Synpcc7942_2126 at 2206.742 kb on - strand, within Synpcc7942_2126 at 2206.817 kb on + strand at 2206.817 kb on + strand at 2206.822 kb on - strand at 2206.825 kb on - strand at 2206.825 kb on - strand at 2206.836 kb on + strand at 2206.838 kb on - strand at 2206.844 kb on - strand at 2206.858 kb on + strand at 2206.858 kb on + strand at 2206.858 kb on + strand at 2206.858 kb on + strand at 2206.885 kb on + strand at 2206.888 kb on + strand at 2206.888 kb on + strand at 2206.894 kb on - strand at 2206.935 kb on - strand at 2206.955 kb on + strand at 2207.137 kb on - strand at 2207.152 kb on + strand at 2207.152 kb on + strand at 2207.164 kb on + strand at 2207.198 kb on + strand, within nblA at 2207.206 kb on - strand, within nblA at 2207.228 kb on - strand, within nblA at 2207.231 kb on + strand, within nblA at 2207.239 kb on + strand, within nblA at 2207.305 kb on + strand, within nblA at 2207.305 kb on + strand, within nblA at 2207.323 kb on - strand, within nblA at 2207.340 kb on + strand at 2207.342 kb on + strand at 2207.348 kb on - strand at 2207.350 kb on - strand at 2207.352 kb on + strand at 2207.360 kb on - strand at 2207.360 kb on - strand at 2207.380 kb on - strand at 2207.420 kb on - strand at 2207.431 kb on - strand at 2207.443 kb on + strand at 2207.491 kb on + strand at 2207.494 kb on - strand at 2207.499 kb on - strand at 2207.511 kb on - strand at 2207.521 kb on + strand at 2207.626 kb on - strand, within txlA at 2207.626 kb on - strand, within txlA at 2207.626 kb on - strand, within txlA
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,205,482 - bcp Synpcc7942_2124 0.59 -3.3 2,205,500 - bcp Synpcc7942_2124 0.61 -1.4 2,205,601 - bcp Synpcc7942_2124 0.71 -2.8 2,205,732 - bcp Synpcc7942_2124 0.85 -0.6 2,205,732 - bcp Synpcc7942_2124 0.85 -1.8 2,205,745 - bcp Synpcc7942_2124 0.86 -2.9 2,205,865 - -0.7 2,205,874 + +0.6 2,205,874 + +1.4 2,205,881 + +2.5 2,205,905 + -2.3 2,205,905 + -0.1 2,205,913 - +0.0 2,205,925 + -2.1 2,205,925 + +0.6 2,205,930 + +1.2 2,205,935 + -1.8 2,205,935 + -1.0 2,205,938 - -2.0 2,205,940 + -2.2 2,205,943 - -2.1 2,205,943 - +1.5 2,205,950 - -2.5 2,205,961 + +3.4 2,205,962 + +4.6 2,205,962 + -1.9 2,205,962 + -1.8 2,205,970 - -2.0 2,205,970 - -2.1 2,205,970 - -0.1 2,205,970 - +1.9 2,205,985 + +0.9 2,205,987 + +1.4 2,206,005 - +0.6 2,206,005 - +1.4 2,206,017 - -1.3 2,206,034 - +2.0 2,206,038 + +0.6 2,206,046 + Synpcc7942_2125 0.11 +0.9 2,206,065 - Synpcc7942_2125 0.16 +0.2 2,206,112 + Synpcc7942_2125 0.27 -1.4 2,206,114 + Synpcc7942_2125 0.28 +0.9 2,206,127 - +0.0 2,206,132 - Synpcc7942_2125 0.32 -3.8 2,206,132 - Synpcc7942_2125 0.32 +0.0 2,206,151 - Synpcc7942_2125 0.37 +2.1 2,206,155 - Synpcc7942_2125 0.38 -0.4 2,206,185 - Synpcc7942_2125 0.46 +3.0 2,206,192 + Synpcc7942_2125 0.47 -0.5 2,206,200 - Synpcc7942_2125 0.49 +1.2 2,206,200 - Synpcc7942_2125 0.49 -2.3 2,206,210 - Synpcc7942_2125 0.52 -0.4 2,206,245 + Synpcc7942_2125 0.60 +1.6 2,206,245 + Synpcc7942_2125 0.60 -1.7 2,206,253 - Synpcc7942_2125 0.62 -1.1 2,206,267 + Synpcc7942_2125 0.66 +1.4 2,206,267 + Synpcc7942_2125 0.66 +1.8 2,206,267 + Synpcc7942_2125 0.66 +1.4 2,206,290 + -1.0 2,206,291 + Synpcc7942_2125 0.72 +0.5 2,206,316 - Synpcc7942_2125 0.78 -1.4 2,206,373 - -1.3 2,206,395 - -0.8 2,206,398 + -0.4 2,206,431 - -4.5 2,206,433 + -2.1 2,206,433 + -1.3 2,206,433 + -2.7 2,206,433 + +0.1 2,206,441 - Synpcc7942_2126 0.10 -2.7 2,206,441 - Synpcc7942_2126 0.10 -2.8 2,206,441 - Synpcc7942_2126 0.10 -1.0 2,206,441 - Synpcc7942_2126 0.10 -1.6 2,206,452 - Synpcc7942_2126 0.13 -3.0 2,206,456 + Synpcc7942_2126 0.14 -2.2 2,206,464 - Synpcc7942_2126 0.16 -1.7 2,206,471 - Synpcc7942_2126 0.18 -0.9 2,206,519 - Synpcc7942_2126 0.29 -1.1 2,206,519 - Synpcc7942_2126 0.29 -1.3 2,206,524 + Synpcc7942_2126 0.31 -3.8 2,206,532 - Synpcc7942_2126 0.33 -1.7 2,206,555 + Synpcc7942_2126 0.38 -2.4 2,206,589 - Synpcc7942_2126 0.47 -3.1 2,206,600 - Synpcc7942_2126 0.49 -1.4 2,206,614 - Synpcc7942_2126 0.53 -3.4 2,206,683 + Synpcc7942_2126 0.70 -1.9 2,206,683 + Synpcc7942_2126 0.70 -0.1 2,206,683 + Synpcc7942_2126 0.70 -2.9 2,206,691 - Synpcc7942_2126 0.72 -2.3 2,206,691 - Synpcc7942_2126 0.72 -3.9 2,206,691 - Synpcc7942_2126 0.72 -2.8 2,206,693 + Synpcc7942_2126 0.72 -0.6 2,206,700 - Synpcc7942_2126 0.74 -4.2 2,206,717 - Synpcc7942_2126 0.78 -2.9 2,206,728 - Synpcc7942_2126 0.81 -3.9 2,206,728 - Synpcc7942_2126 0.81 -3.8 2,206,734 + Synpcc7942_2126 0.82 -2.5 2,206,742 - Synpcc7942_2126 0.84 -1.6 2,206,742 - Synpcc7942_2126 0.84 +1.3 2,206,742 - Synpcc7942_2126 0.84 -3.4 2,206,817 + +1.7 2,206,817 + -2.0 2,206,822 - -0.1 2,206,825 - -1.0 2,206,825 - +2.9 2,206,836 + +2.9 2,206,838 - +0.9 2,206,844 - -0.2 2,206,858 + -1.7 2,206,858 + +0.7 2,206,858 + +1.3 2,206,858 + -0.7 2,206,885 + +0.3 2,206,888 + +2.0 2,206,888 + -1.6 2,206,894 - -0.1 2,206,935 - +0.4 2,206,955 + -0.2 2,207,137 - -2.4 2,207,152 + +2.9 2,207,152 + +2.2 2,207,164 + +0.2 2,207,198 + nblA Synpcc7942_2127 0.17 -1.6 2,207,206 - nblA Synpcc7942_2127 0.22 +0.3 2,207,228 - nblA Synpcc7942_2127 0.34 +3.0 2,207,231 + nblA Synpcc7942_2127 0.36 +0.6 2,207,239 + nblA Synpcc7942_2127 0.40 -2.0 2,207,305 + nblA Synpcc7942_2127 0.77 -0.6 2,207,305 + nblA Synpcc7942_2127 0.77 +0.7 2,207,323 - nblA Synpcc7942_2127 0.87 -2.0 2,207,340 + +0.3 2,207,342 + +1.2 2,207,348 - +1.7 2,207,350 - +1.1 2,207,352 + +0.1 2,207,360 - -1.6 2,207,360 - +3.0 2,207,380 - -0.9 2,207,420 - -1.5 2,207,431 - -0.9 2,207,443 + -0.8 2,207,491 + -0.6 2,207,494 - -2.7 2,207,499 - +2.9 2,207,511 - -0.3 2,207,521 + +0.9 2,207,626 - txlA Synpcc7942_2128 0.21 -0.1 2,207,626 - txlA Synpcc7942_2128 0.21 +5.2 2,207,626 - txlA Synpcc7942_2128 0.21 +4.6
Or see this region's nucleotide sequence