Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaJ1 and Synpcc7942_2075 overlap by 4 nucleotides Synpcc7942_2075 and rsgA overlap by 4 nucleotides rsgA and Synpcc7942_2077 are separated by 66 nucleotides Synpcc7942_2077 and pgam are separated by 377 nucleotides
Synpcc7942_2074: dnaJ1 - Heat shock protein DnaJ, at 2,152,639 to 2,153,769
dnaJ1
Synpcc7942_2075: Synpcc7942_2075 - hypothetical protein, at 2,153,766 to 2,154,017
_2075
Synpcc7942_2076: rsgA - ribosome-associated GTPase, at 2,154,014 to 2,155,066
rsgA
Synpcc7942_2077: Synpcc7942_2077 - hypothetical protein, at 2,155,133 to 2,155,549
_2077
Synpcc7942_2078: pgam - phosphoglycerate mutase, at 2,155,927 to 2,156,565
pgam
Position (kb)
2154
2155
2156 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2153.149 kb on - strand, within dnaJ1 at 2153.449 kb on - strand, within dnaJ1 at 2153.451 kb on + strand, within dnaJ1 at 2153.484 kb on - strand, within dnaJ1 at 2153.522 kb on + strand, within dnaJ1 at 2153.580 kb on + strand, within dnaJ1 at 2153.580 kb on + strand, within dnaJ1 at 2153.580 kb on + strand, within dnaJ1 at 2153.729 kb on - strand at 2154.008 kb on - strand at 2154.031 kb on - strand at 2154.058 kb on + strand at 2154.058 kb on + strand at 2154.058 kb on + strand at 2154.066 kb on - strand at 2154.079 kb on - strand at 2154.150 kb on + strand, within rsgA at 2154.151 kb on - strand, within rsgA at 2154.192 kb on + strand, within rsgA at 2154.250 kb on - strand, within rsgA at 2154.315 kb on + strand, within rsgA at 2154.354 kb on + strand, within rsgA at 2154.360 kb on + strand, within rsgA at 2154.360 kb on + strand, within rsgA at 2154.360 kb on + strand, within rsgA at 2154.360 kb on + strand, within rsgA at 2154.365 kb on + strand, within rsgA at 2154.365 kb on + strand, within rsgA at 2154.368 kb on - strand, within rsgA at 2154.455 kb on - strand, within rsgA at 2154.464 kb on + strand, within rsgA at 2154.472 kb on - strand, within rsgA at 2154.582 kb on + strand, within rsgA at 2154.604 kb on + strand, within rsgA at 2154.615 kb on + strand, within rsgA at 2154.616 kb on + strand, within rsgA at 2154.664 kb on + strand, within rsgA at 2154.673 kb on - strand, within rsgA at 2154.715 kb on + strand, within rsgA at 2154.718 kb on - strand, within rsgA at 2154.723 kb on - strand, within rsgA at 2154.826 kb on + strand, within rsgA at 2155.037 kb on + strand at 2155.047 kb on + strand at 2155.055 kb on - strand at 2155.092 kb on - strand at 2155.157 kb on + strand at 2155.158 kb on - strand at 2155.165 kb on - strand at 2155.170 kb on - strand at 2155.177 kb on - strand, within Synpcc7942_2077 at 2155.178 kb on + strand, within Synpcc7942_2077 at 2155.188 kb on + strand, within Synpcc7942_2077 at 2155.193 kb on + strand, within Synpcc7942_2077 at 2155.317 kb on + strand, within Synpcc7942_2077 at 2155.338 kb on + strand, within Synpcc7942_2077 at 2155.372 kb on + strand, within Synpcc7942_2077 at 2155.409 kb on + strand, within Synpcc7942_2077 at 2155.448 kb on + strand at 2155.456 kb on + strand, within Synpcc7942_2077 at 2155.473 kb on + strand, within Synpcc7942_2077 at 2155.498 kb on - strand, within Synpcc7942_2077 at 2155.520 kb on + strand at 2155.539 kb on + strand at 2155.551 kb on - strand at 2155.559 kb on + strand at 2155.559 kb on + strand at 2155.572 kb on + strand at 2155.616 kb on + strand at 2155.776 kb on + strand at 2155.776 kb on + strand at 2155.776 kb on + strand at 2155.776 kb on + strand at 2155.776 kb on + strand at 2155.776 kb on + strand at 2155.776 kb on + strand at 2155.776 kb on + strand at 2155.778 kb on + strand at 2155.784 kb on - strand at 2155.784 kb on - strand at 2155.784 kb on - strand at 2155.785 kb on - strand at 2155.786 kb on - strand at 2155.788 kb on + strand at 2155.790 kb on + strand at 2155.791 kb on - strand at 2155.798 kb on - strand at 2155.813 kb on + strand at 2155.817 kb on - strand at 2155.821 kb on - strand at 2155.878 kb on + strand at 2155.919 kb on + strand at 2155.991 kb on - strand, within pgam at 2155.994 kb on + strand, within pgam at 2156.016 kb on + strand, within pgam at 2156.032 kb on + strand, within pgam
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,153,149 - dnaJ1 Synpcc7942_2074 0.45 +2.4 2,153,449 - dnaJ1 Synpcc7942_2074 0.72 -1.0 2,153,451 + dnaJ1 Synpcc7942_2074 0.72 -1.9 2,153,484 - dnaJ1 Synpcc7942_2074 0.75 -2.6 2,153,522 + dnaJ1 Synpcc7942_2074 0.78 +2.1 2,153,580 + dnaJ1 Synpcc7942_2074 0.83 -0.3 2,153,580 + dnaJ1 Synpcc7942_2074 0.83 -2.6 2,153,580 + dnaJ1 Synpcc7942_2074 0.83 +0.4 2,153,729 - -2.0 2,154,008 - +2.1 2,154,031 - -2.3 2,154,058 + -1.6 2,154,058 + -2.1 2,154,058 + -0.8 2,154,066 - -2.8 2,154,079 - +0.4 2,154,150 + rsgA Synpcc7942_2076 0.13 +1.0 2,154,151 - rsgA Synpcc7942_2076 0.13 -2.0 2,154,192 + rsgA Synpcc7942_2076 0.17 -0.8 2,154,250 - rsgA Synpcc7942_2076 0.22 -3.1 2,154,315 + rsgA Synpcc7942_2076 0.29 -0.3 2,154,354 + rsgA Synpcc7942_2076 0.32 -1.6 2,154,360 + rsgA Synpcc7942_2076 0.33 -1.6 2,154,360 + rsgA Synpcc7942_2076 0.33 -2.2 2,154,360 + rsgA Synpcc7942_2076 0.33 -1.3 2,154,360 + rsgA Synpcc7942_2076 0.33 -4.6 2,154,365 + rsgA Synpcc7942_2076 0.33 -2.0 2,154,365 + rsgA Synpcc7942_2076 0.33 -0.8 2,154,368 - rsgA Synpcc7942_2076 0.34 -1.6 2,154,455 - rsgA Synpcc7942_2076 0.42 -0.8 2,154,464 + rsgA Synpcc7942_2076 0.43 -3.0 2,154,472 - rsgA Synpcc7942_2076 0.43 +0.4 2,154,582 + rsgA Synpcc7942_2076 0.54 -3.0 2,154,604 + rsgA Synpcc7942_2076 0.56 -1.2 2,154,615 + rsgA Synpcc7942_2076 0.57 -2.8 2,154,616 + rsgA Synpcc7942_2076 0.57 -2.6 2,154,664 + rsgA Synpcc7942_2076 0.62 -1.2 2,154,673 - rsgA Synpcc7942_2076 0.63 -0.6 2,154,715 + rsgA Synpcc7942_2076 0.67 -3.0 2,154,718 - rsgA Synpcc7942_2076 0.67 -2.3 2,154,723 - rsgA Synpcc7942_2076 0.67 +1.0 2,154,826 + rsgA Synpcc7942_2076 0.77 -2.8 2,155,037 + -4.7 2,155,047 + -1.2 2,155,055 - -0.7 2,155,092 - +0.4 2,155,157 + +0.0 2,155,158 - -2.4 2,155,165 - -1.3 2,155,170 - -2.2 2,155,177 - Synpcc7942_2077 0.11 +1.7 2,155,178 + Synpcc7942_2077 0.11 -1.0 2,155,188 + Synpcc7942_2077 0.13 +0.2 2,155,193 + Synpcc7942_2077 0.14 -0.8 2,155,317 + Synpcc7942_2077 0.44 -2.4 2,155,338 + Synpcc7942_2077 0.49 +1.5 2,155,372 + Synpcc7942_2077 0.57 +0.3 2,155,409 + Synpcc7942_2077 0.66 +0.9 2,155,448 + -1.2 2,155,456 + Synpcc7942_2077 0.77 +3.5 2,155,473 + Synpcc7942_2077 0.82 -1.5 2,155,498 - Synpcc7942_2077 0.88 -2.2 2,155,520 + +1.5 2,155,539 + -2.0 2,155,551 - +0.1 2,155,559 + -2.2 2,155,559 + -0.9 2,155,572 + +0.7 2,155,616 + -0.2 2,155,776 + -1.6 2,155,776 + +2.3 2,155,776 + -2.5 2,155,776 + +1.0 2,155,776 + -1.2 2,155,776 + +3.7 2,155,776 + -0.4 2,155,776 + -2.4 2,155,778 + -1.1 2,155,784 - +0.6 2,155,784 - +0.3 2,155,784 - -0.8 2,155,785 - -3.1 2,155,786 - -2.2 2,155,788 + -0.2 2,155,790 + +0.7 2,155,791 - +0.7 2,155,798 - +1.7 2,155,813 + -1.6 2,155,817 - -1.7 2,155,821 - -1.2 2,155,878 + +1.3 2,155,919 + +0.4 2,155,991 - pgam Synpcc7942_2078 0.10 -3.1 2,155,994 + pgam Synpcc7942_2078 0.10 +0.9 2,156,016 + pgam Synpcc7942_2078 0.14 +3.6 2,156,032 + pgam Synpcc7942_2078 0.16 +0.2
Or see this region's nucleotide sequence