Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sigI and Synpcc7942_2005 are separated by 9 nucleotides Synpcc7942_2005 and cdv3 are separated by 148 nucleotides cdv3 and coaD overlap by 4 nucleotides coaD and Synpcc7942_2008 are separated by 89 nucleotides
Synpcc7942_2004: sigI - RNA polymerase sigma factor, at 2,073,028 to 2,073,618
sigI
Synpcc7942_2005: Synpcc7942_2005 - flm3 region hypothetical protein 4, at 2,073,628 to 2,074,449
_2005
Synpcc7942_2006: cdv3 - hypothetical protein, at 2,074,598 to 2,075,284
cdv3
Synpcc7942_2007: coaD - phosphopantetheine adenylyltransferase, at 2,075,281 to 2,075,781
coaD
Synpcc7942_2008: Synpcc7942_2008 - hypothetical protein, at 2,075,871 to 2,076,353
_2008
Position (kb)
2074
2075
2076 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2073.605 kb on - strand at 2073.607 kb on + strand at 2073.668 kb on + strand at 2073.673 kb on + strand at 2073.681 kb on - strand at 2073.682 kb on - strand at 2073.688 kb on - strand at 2073.703 kb on - strand at 2073.713 kb on + strand, within Synpcc7942_2005 at 2073.737 kb on + strand at 2073.742 kb on + strand, within Synpcc7942_2005 at 2073.745 kb on - strand, within Synpcc7942_2005 at 2073.752 kb on - strand, within Synpcc7942_2005 at 2073.764 kb on - strand, within Synpcc7942_2005 at 2073.821 kb on + strand, within Synpcc7942_2005 at 2073.835 kb on + strand, within Synpcc7942_2005 at 2073.855 kb on - strand, within Synpcc7942_2005 at 2073.858 kb on + strand, within Synpcc7942_2005 at 2073.861 kb on - strand, within Synpcc7942_2005 at 2073.936 kb on - strand, within Synpcc7942_2005 at 2073.939 kb on + strand, within Synpcc7942_2005 at 2073.952 kb on + strand, within Synpcc7942_2005 at 2073.960 kb on - strand, within Synpcc7942_2005 at 2073.960 kb on - strand, within Synpcc7942_2005 at 2073.981 kb on - strand, within Synpcc7942_2005 at 2074.007 kb on + strand, within Synpcc7942_2005 at 2074.014 kb on - strand, within Synpcc7942_2005 at 2074.028 kb on + strand, within Synpcc7942_2005 at 2074.030 kb on + strand, within Synpcc7942_2005 at 2074.051 kb on + strand, within Synpcc7942_2005 at 2074.051 kb on + strand, within Synpcc7942_2005 at 2074.124 kb on + strand, within Synpcc7942_2005 at 2074.124 kb on + strand, within Synpcc7942_2005 at 2074.124 kb on + strand, within Synpcc7942_2005 at 2074.124 kb on + strand, within Synpcc7942_2005 at 2074.124 kb on + strand, within Synpcc7942_2005 at 2074.132 kb on - strand, within Synpcc7942_2005 at 2074.132 kb on - strand, within Synpcc7942_2005 at 2074.132 kb on - strand, within Synpcc7942_2005 at 2074.138 kb on + strand, within Synpcc7942_2005 at 2074.206 kb on - strand, within Synpcc7942_2005 at 2074.224 kb on + strand, within Synpcc7942_2005 at 2074.232 kb on - strand, within Synpcc7942_2005 at 2074.279 kb on + strand, within Synpcc7942_2005 at 2074.316 kb on + strand, within Synpcc7942_2005 at 2074.334 kb on - strand, within Synpcc7942_2005 at 2074.339 kb on - strand, within Synpcc7942_2005 at 2074.373 kb on - strand at 2074.406 kb on + strand at 2074.451 kb on - strand at 2074.460 kb on - strand at 2074.470 kb on - strand at 2074.470 kb on - strand at 2074.482 kb on + strand at 2074.501 kb on + strand at 2074.501 kb on + strand at 2074.509 kb on - strand at 2074.509 kb on - strand at 2074.509 kb on - strand at 2074.509 kb on - strand at 2074.536 kb on - strand at 2074.538 kb on + strand at 2074.548 kb on + strand at 2074.548 kb on + strand at 2074.548 kb on + strand at 2074.548 kb on + strand at 2074.548 kb on + strand at 2074.550 kb on + strand at 2074.556 kb on - strand at 2074.556 kb on - strand at 2074.556 kb on - strand at 2074.575 kb on - strand at 2074.621 kb on - strand at 2074.662 kb on + strand at 2074.699 kb on + strand, within cdv3 at 2074.707 kb on - strand, within cdv3 at 2074.709 kb on - strand, within cdv3 at 2074.731 kb on - strand, within cdv3 at 2074.733 kb on - strand, within cdv3 at 2074.791 kb on - strand, within cdv3 at 2074.809 kb on - strand, within cdv3 at 2074.885 kb on + strand, within cdv3 at 2074.903 kb on + strand, within cdv3 at 2074.909 kb on - strand, within cdv3 at 2074.911 kb on - strand, within cdv3 at 2074.937 kb on + strand, within cdv3 at 2074.951 kb on + strand, within cdv3 at 2074.967 kb on + strand, within cdv3 at 2075.001 kb on + strand, within cdv3 at 2075.014 kb on - strand, within cdv3 at 2075.034 kb on + strand, within cdv3 at 2075.116 kb on - strand, within cdv3 at 2075.242 kb on + strand at 2075.242 kb on + strand at 2075.250 kb on - strand at 2075.250 kb on - strand at 2075.250 kb on - strand at 2075.250 kb on - strand at 2075.250 kb on - strand at 2075.252 kb on + strand at 2075.800 kb on + strand at 2075.820 kb on - strand at 2075.838 kb on + strand at 2075.839 kb on + strand at 2075.839 kb on + strand at 2075.839 kb on + strand at 2075.846 kb on + strand at 2075.846 kb on - strand at 2075.847 kb on - strand at 2075.875 kb on - strand at 2075.920 kb on + strand, within Synpcc7942_2008 at 2075.920 kb on + strand, within Synpcc7942_2008 at 2075.920 kb on + strand, within Synpcc7942_2008 at 2075.920 kb on + strand, within Synpcc7942_2008 at 2075.920 kb on + strand, within Synpcc7942_2008 at 2075.920 kb on + strand, within Synpcc7942_2008 at 2075.920 kb on + strand, within Synpcc7942_2008 at 2075.922 kb on + strand, within Synpcc7942_2008 at 2075.928 kb on - strand, within Synpcc7942_2008 at 2075.928 kb on - strand, within Synpcc7942_2008 at 2075.928 kb on - strand, within Synpcc7942_2008 at 2075.928 kb on - strand, within Synpcc7942_2008 at 2075.928 kb on - strand, within Synpcc7942_2008 at 2075.951 kb on + strand, within Synpcc7942_2008 at 2075.954 kb on + strand, within Synpcc7942_2008 at 2075.962 kb on - strand, within Synpcc7942_2008 at 2075.967 kb on + strand, within Synpcc7942_2008 at 2075.970 kb on - strand, within Synpcc7942_2008 at 2075.979 kb on + strand, within Synpcc7942_2008 at 2076.009 kb on + strand, within Synpcc7942_2008 at 2076.011 kb on - strand, within Synpcc7942_2008 at 2076.040 kb on + strand, within Synpcc7942_2008 at 2076.050 kb on + strand, within Synpcc7942_2008 at 2076.050 kb on + strand, within Synpcc7942_2008 at 2076.053 kb on - strand, within Synpcc7942_2008 at 2076.082 kb on + strand, within Synpcc7942_2008 at 2076.101 kb on + strand, within Synpcc7942_2008 at 2076.167 kb on - strand, within Synpcc7942_2008 at 2076.170 kb on + strand, within Synpcc7942_2008 at 2076.196 kb on + strand, within Synpcc7942_2008 at 2076.207 kb on + strand at 2076.207 kb on + strand, within Synpcc7942_2008 at 2076.215 kb on - strand, within Synpcc7942_2008 at 2076.220 kb on - strand, within Synpcc7942_2008 at 2076.226 kb on + strand, within Synpcc7942_2008 at 2076.227 kb on - strand, within Synpcc7942_2008 at 2076.236 kb on + strand, within Synpcc7942_2008 at 2076.249 kb on - strand, within Synpcc7942_2008 at 2076.264 kb on + strand, within Synpcc7942_2008 at 2076.264 kb on + strand, within Synpcc7942_2008
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,073,605 - -0.1 2,073,607 + -3.1 2,073,668 + +1.1 2,073,673 + +2.2 2,073,681 - +1.0 2,073,682 - +2.7 2,073,688 - +2.3 2,073,703 - +0.7 2,073,713 + Synpcc7942_2005 0.10 -2.2 2,073,737 + +0.0 2,073,742 + Synpcc7942_2005 0.14 +1.0 2,073,745 - Synpcc7942_2005 0.14 +0.8 2,073,752 - Synpcc7942_2005 0.15 -0.0 2,073,764 - Synpcc7942_2005 0.17 +2.1 2,073,821 + Synpcc7942_2005 0.23 -1.7 2,073,835 + Synpcc7942_2005 0.25 +1.0 2,073,855 - Synpcc7942_2005 0.28 +1.3 2,073,858 + Synpcc7942_2005 0.28 +0.6 2,073,861 - Synpcc7942_2005 0.28 +0.5 2,073,936 - Synpcc7942_2005 0.37 -2.0 2,073,939 + Synpcc7942_2005 0.38 -0.1 2,073,952 + Synpcc7942_2005 0.39 +0.6 2,073,960 - Synpcc7942_2005 0.40 -3.4 2,073,960 - Synpcc7942_2005 0.40 -0.9 2,073,981 - Synpcc7942_2005 0.43 -0.9 2,074,007 + Synpcc7942_2005 0.46 -0.8 2,074,014 - Synpcc7942_2005 0.47 -0.2 2,074,028 + Synpcc7942_2005 0.49 -1.1 2,074,030 + Synpcc7942_2005 0.49 -1.0 2,074,051 + Synpcc7942_2005 0.51 +0.2 2,074,051 + Synpcc7942_2005 0.51 -0.7 2,074,124 + Synpcc7942_2005 0.60 -0.4 2,074,124 + Synpcc7942_2005 0.60 +0.7 2,074,124 + Synpcc7942_2005 0.60 -1.0 2,074,124 + Synpcc7942_2005 0.60 -0.6 2,074,124 + Synpcc7942_2005 0.60 -0.5 2,074,132 - Synpcc7942_2005 0.61 +2.4 2,074,132 - Synpcc7942_2005 0.61 +0.1 2,074,132 - Synpcc7942_2005 0.61 +4.0 2,074,138 + Synpcc7942_2005 0.62 +2.4 2,074,206 - Synpcc7942_2005 0.70 +2.2 2,074,224 + Synpcc7942_2005 0.73 -1.3 2,074,232 - Synpcc7942_2005 0.73 -2.6 2,074,279 + Synpcc7942_2005 0.79 +2.4 2,074,316 + Synpcc7942_2005 0.84 -3.1 2,074,334 - Synpcc7942_2005 0.86 -1.3 2,074,339 - Synpcc7942_2005 0.86 -0.3 2,074,373 - +0.1 2,074,406 + -0.3 2,074,451 - -1.7 2,074,460 - +1.2 2,074,470 - -0.4 2,074,470 - -0.8 2,074,482 + -1.1 2,074,501 + -0.0 2,074,501 + +1.9 2,074,509 - +2.0 2,074,509 - -1.0 2,074,509 - +3.6 2,074,509 - +2.7 2,074,536 - +1.2 2,074,538 + -0.8 2,074,548 + +1.5 2,074,548 + -1.1 2,074,548 + -2.4 2,074,548 + +0.8 2,074,548 + -1.7 2,074,550 + -2.3 2,074,556 - -1.1 2,074,556 - +1.8 2,074,556 - -1.0 2,074,575 - +1.5 2,074,621 - +0.0 2,074,662 + -1.1 2,074,699 + cdv3 Synpcc7942_2006 0.15 -3.1 2,074,707 - cdv3 Synpcc7942_2006 0.16 -1.3 2,074,709 - cdv3 Synpcc7942_2006 0.16 -2.2 2,074,731 - cdv3 Synpcc7942_2006 0.19 -0.5 2,074,733 - cdv3 Synpcc7942_2006 0.20 -3.5 2,074,791 - cdv3 Synpcc7942_2006 0.28 -3.9 2,074,809 - cdv3 Synpcc7942_2006 0.31 -1.0 2,074,885 + cdv3 Synpcc7942_2006 0.42 -3.1 2,074,903 + cdv3 Synpcc7942_2006 0.44 -4.2 2,074,909 - cdv3 Synpcc7942_2006 0.45 -2.2 2,074,911 - cdv3 Synpcc7942_2006 0.46 -0.9 2,074,937 + cdv3 Synpcc7942_2006 0.49 -5.4 2,074,951 + cdv3 Synpcc7942_2006 0.51 -3.0 2,074,967 + cdv3 Synpcc7942_2006 0.54 -0.5 2,075,001 + cdv3 Synpcc7942_2006 0.59 -2.1 2,075,014 - cdv3 Synpcc7942_2006 0.61 -1.0 2,075,034 + cdv3 Synpcc7942_2006 0.63 -1.7 2,075,116 - cdv3 Synpcc7942_2006 0.75 -1.1 2,075,242 + -0.3 2,075,242 + -1.4 2,075,250 - -2.1 2,075,250 - -2.0 2,075,250 - -0.8 2,075,250 - -2.2 2,075,250 - -2.3 2,075,252 + -3.6 2,075,800 + +0.2 2,075,820 - +0.6 2,075,838 + -1.4 2,075,839 + -1.1 2,075,839 + +2.2 2,075,839 + -1.3 2,075,846 + +1.0 2,075,846 - +3.2 2,075,847 - -2.3 2,075,875 - -0.3 2,075,920 + Synpcc7942_2008 0.10 -2.5 2,075,920 + Synpcc7942_2008 0.10 +1.8 2,075,920 + Synpcc7942_2008 0.10 +0.6 2,075,920 + Synpcc7942_2008 0.10 -0.4 2,075,920 + Synpcc7942_2008 0.10 +2.7 2,075,920 + Synpcc7942_2008 0.10 -1.9 2,075,920 + Synpcc7942_2008 0.10 -3.4 2,075,922 + Synpcc7942_2008 0.11 -1.0 2,075,928 - Synpcc7942_2008 0.12 +0.9 2,075,928 - Synpcc7942_2008 0.12 +1.8 2,075,928 - Synpcc7942_2008 0.12 -0.6 2,075,928 - Synpcc7942_2008 0.12 -2.2 2,075,928 - Synpcc7942_2008 0.12 +2.2 2,075,951 + Synpcc7942_2008 0.17 -1.6 2,075,954 + Synpcc7942_2008 0.17 +1.5 2,075,962 - Synpcc7942_2008 0.19 -1.8 2,075,967 + Synpcc7942_2008 0.20 +1.5 2,075,970 - Synpcc7942_2008 0.20 -1.3 2,075,979 + Synpcc7942_2008 0.22 -1.1 2,076,009 + Synpcc7942_2008 0.29 +0.8 2,076,011 - Synpcc7942_2008 0.29 -2.1 2,076,040 + Synpcc7942_2008 0.35 -2.3 2,076,050 + Synpcc7942_2008 0.37 -0.1 2,076,050 + Synpcc7942_2008 0.37 -1.4 2,076,053 - Synpcc7942_2008 0.38 +1.5 2,076,082 + Synpcc7942_2008 0.44 +1.6 2,076,101 + Synpcc7942_2008 0.48 -1.2 2,076,167 - Synpcc7942_2008 0.61 -2.7 2,076,170 + Synpcc7942_2008 0.62 +1.0 2,076,196 + Synpcc7942_2008 0.67 -2.6 2,076,207 + -1.7 2,076,207 + Synpcc7942_2008 0.70 +1.6 2,076,215 - Synpcc7942_2008 0.71 -2.5 2,076,220 - Synpcc7942_2008 0.72 +0.2 2,076,226 + Synpcc7942_2008 0.73 -2.0 2,076,227 - Synpcc7942_2008 0.74 +0.7 2,076,236 + Synpcc7942_2008 0.76 -1.3 2,076,249 - Synpcc7942_2008 0.78 +1.0 2,076,264 + Synpcc7942_2008 0.81 +2.0 2,076,264 + Synpcc7942_2008 0.81 +1.2
Or see this region's nucleotide sequence