Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1998 and rbp2 are separated by 304 nucleotides rbp2 and mrcB are separated by 222 nucleotides
Synpcc7942_1998: Synpcc7942_1998 - GAF, at 2,066,556 to 2,067,143
_1998
Synpcc7942_1999: rbp2 - RNA-binding region RNP-1, at 2,067,448 to 2,067,735
rbp2
Synpcc7942_2000: mrcB - Penicillin-binding protein 1A, at 2,067,958 to 2,070,180
mrcB
Position (kb)
2067
2068 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2066.463 kb on + strand at 2066.532 kb on - strand at 2066.539 kb on - strand at 2066.539 kb on - strand at 2066.552 kb on + strand at 2066.552 kb on + strand at 2066.594 kb on + strand at 2066.625 kb on + strand, within Synpcc7942_1998 at 2066.625 kb on + strand, within Synpcc7942_1998 at 2066.625 kb on + strand, within Synpcc7942_1998 at 2066.630 kb on + strand, within Synpcc7942_1998 at 2066.633 kb on - strand, within Synpcc7942_1998 at 2066.633 kb on - strand, within Synpcc7942_1998 at 2066.635 kb on + strand, within Synpcc7942_1998 at 2066.641 kb on - strand, within Synpcc7942_1998 at 2066.641 kb on - strand, within Synpcc7942_1998 at 2066.643 kb on - strand, within Synpcc7942_1998 at 2066.657 kb on + strand, within Synpcc7942_1998 at 2066.681 kb on + strand, within Synpcc7942_1998 at 2066.689 kb on + strand at 2066.712 kb on - strand, within Synpcc7942_1998 at 2066.723 kb on - strand, within Synpcc7942_1998 at 2066.739 kb on - strand, within Synpcc7942_1998 at 2066.744 kb on - strand, within Synpcc7942_1998 at 2066.749 kb on - strand, within Synpcc7942_1998 at 2066.762 kb on - strand, within Synpcc7942_1998 at 2066.789 kb on + strand, within Synpcc7942_1998 at 2066.797 kb on - strand, within Synpcc7942_1998 at 2066.810 kb on + strand, within Synpcc7942_1998 at 2066.813 kb on - strand, within Synpcc7942_1998 at 2066.830 kb on + strand, within Synpcc7942_1998 at 2066.836 kb on - strand, within Synpcc7942_1998 at 2066.850 kb on + strand, within Synpcc7942_1998 at 2066.875 kb on + strand, within Synpcc7942_1998 at 2066.876 kb on + strand, within Synpcc7942_1998 at 2066.881 kb on + strand, within Synpcc7942_1998 at 2066.889 kb on - strand, within Synpcc7942_1998 at 2066.892 kb on + strand, within Synpcc7942_1998 at 2066.901 kb on + strand, within Synpcc7942_1998 at 2066.929 kb on - strand, within Synpcc7942_1998 at 2066.968 kb on - strand at 2066.968 kb on - strand, within Synpcc7942_1998 at 2066.968 kb on - strand, within Synpcc7942_1998 at 2066.968 kb on - strand, within Synpcc7942_1998 at 2067.002 kb on - strand, within Synpcc7942_1998 at 2067.008 kb on + strand, within Synpcc7942_1998 at 2067.025 kb on + strand, within Synpcc7942_1998 at 2067.039 kb on + strand, within Synpcc7942_1998 at 2067.044 kb on + strand, within Synpcc7942_1998 at 2067.052 kb on - strand, within Synpcc7942_1998 at 2067.052 kb on - strand, within Synpcc7942_1998 at 2067.076 kb on - strand, within Synpcc7942_1998 at 2067.107 kb on - strand at 2067.112 kb on + strand at 2067.178 kb on + strand at 2067.210 kb on + strand at 2067.216 kb on + strand at 2067.223 kb on - strand at 2067.223 kb on - strand at 2067.225 kb on - strand at 2067.236 kb on + strand at 2067.244 kb on - strand at 2067.245 kb on - strand at 2067.246 kb on + strand at 2067.246 kb on + strand at 2067.251 kb on + strand at 2067.251 kb on + strand at 2067.251 kb on + strand at 2067.254 kb on - strand at 2067.259 kb on - strand at 2067.259 kb on - strand at 2067.284 kb on - strand at 2067.293 kb on + strand at 2067.330 kb on - strand at 2067.345 kb on + strand at 2067.345 kb on + strand at 2067.346 kb on - strand at 2067.348 kb on - strand at 2067.348 kb on - strand at 2067.350 kb on + strand at 2067.353 kb on - strand at 2067.378 kb on - strand at 2067.378 kb on - strand at 2067.405 kb on + strand at 2067.424 kb on + strand at 2067.432 kb on - strand at 2067.437 kb on - strand at 2067.447 kb on - strand at 2067.447 kb on - strand at 2067.465 kb on - strand at 2067.536 kb on - strand, within rbp2 at 2067.545 kb on - strand, within rbp2 at 2067.623 kb on + strand, within rbp2 at 2067.659 kb on + strand, within rbp2 at 2067.661 kb on + strand, within rbp2 at 2067.667 kb on - strand, within rbp2 at 2067.667 kb on - strand, within rbp2 at 2067.800 kb on - strand at 2067.845 kb on + strand at 2067.935 kb on + strand at 2067.966 kb on + strand at 2067.966 kb on + strand at 2067.966 kb on + strand at 2067.966 kb on + strand at 2067.974 kb on - strand at 2067.974 kb on - strand at 2067.994 kb on - strand at 2068.122 kb on - strand at 2068.398 kb on + strand, within mrcB at 2068.629 kb on - strand, within mrcB
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,066,463 + +0.3 2,066,532 - -0.6 2,066,539 - -0.4 2,066,539 - -0.9 2,066,552 + -0.1 2,066,552 + +0.2 2,066,594 + -2.0 2,066,625 + Synpcc7942_1998 0.12 -1.1 2,066,625 + Synpcc7942_1998 0.12 +5.3 2,066,625 + Synpcc7942_1998 0.12 +0.0 2,066,630 + Synpcc7942_1998 0.13 +1.0 2,066,633 - Synpcc7942_1998 0.13 +3.0 2,066,633 - Synpcc7942_1998 0.13 +2.6 2,066,635 + Synpcc7942_1998 0.13 +1.5 2,066,641 - Synpcc7942_1998 0.14 -1.7 2,066,641 - Synpcc7942_1998 0.14 +1.4 2,066,643 - Synpcc7942_1998 0.15 +1.7 2,066,657 + Synpcc7942_1998 0.17 -1.3 2,066,681 + Synpcc7942_1998 0.21 +1.2 2,066,689 + -1.0 2,066,712 - Synpcc7942_1998 0.27 -0.8 2,066,723 - Synpcc7942_1998 0.28 -0.6 2,066,739 - Synpcc7942_1998 0.31 +0.0 2,066,744 - Synpcc7942_1998 0.32 -0.9 2,066,749 - Synpcc7942_1998 0.33 -2.0 2,066,762 - Synpcc7942_1998 0.35 +2.5 2,066,789 + Synpcc7942_1998 0.40 -2.3 2,066,797 - Synpcc7942_1998 0.41 -3.0 2,066,810 + Synpcc7942_1998 0.43 -2.2 2,066,813 - Synpcc7942_1998 0.44 +2.9 2,066,830 + Synpcc7942_1998 0.47 -0.7 2,066,836 - Synpcc7942_1998 0.48 -1.1 2,066,850 + Synpcc7942_1998 0.50 +0.9 2,066,875 + Synpcc7942_1998 0.54 +0.0 2,066,876 + Synpcc7942_1998 0.54 +1.0 2,066,881 + Synpcc7942_1998 0.55 -1.3 2,066,889 - Synpcc7942_1998 0.57 -0.1 2,066,892 + Synpcc7942_1998 0.57 +0.3 2,066,901 + Synpcc7942_1998 0.59 -0.2 2,066,929 - Synpcc7942_1998 0.63 +1.5 2,066,968 - +0.4 2,066,968 - Synpcc7942_1998 0.70 +1.0 2,066,968 - Synpcc7942_1998 0.70 -2.0 2,066,968 - Synpcc7942_1998 0.70 -1.6 2,067,002 - Synpcc7942_1998 0.76 +1.6 2,067,008 + Synpcc7942_1998 0.77 +1.7 2,067,025 + Synpcc7942_1998 0.80 -0.4 2,067,039 + Synpcc7942_1998 0.82 -1.5 2,067,044 + Synpcc7942_1998 0.83 +0.9 2,067,052 - Synpcc7942_1998 0.84 +1.5 2,067,052 - Synpcc7942_1998 0.84 +1.6 2,067,076 - Synpcc7942_1998 0.88 +3.5 2,067,107 - -2.3 2,067,112 + +1.7 2,067,178 + -1.4 2,067,210 + +0.0 2,067,216 + -0.5 2,067,223 - +1.3 2,067,223 - +2.3 2,067,225 - -2.1 2,067,236 + +2.6 2,067,244 - -0.0 2,067,245 - +2.8 2,067,246 + -1.0 2,067,246 + -0.2 2,067,251 + -0.2 2,067,251 + -1.4 2,067,251 + -1.6 2,067,254 - +1.0 2,067,259 - -0.9 2,067,259 - +2.2 2,067,284 - -1.6 2,067,293 + +0.0 2,067,330 - +0.9 2,067,345 + -2.9 2,067,345 + -1.0 2,067,346 - +1.9 2,067,348 - +3.0 2,067,348 - -2.7 2,067,350 + +2.5 2,067,353 - -1.4 2,067,378 - +0.3 2,067,378 - +1.5 2,067,405 + -0.5 2,067,424 + +1.0 2,067,432 - -0.8 2,067,437 - -1.0 2,067,447 - +3.5 2,067,447 - -2.1 2,067,465 - -0.5 2,067,536 - rbp2 Synpcc7942_1999 0.31 -1.3 2,067,545 - rbp2 Synpcc7942_1999 0.34 -3.4 2,067,623 + rbp2 Synpcc7942_1999 0.61 -2.2 2,067,659 + rbp2 Synpcc7942_1999 0.73 -1.6 2,067,661 + rbp2 Synpcc7942_1999 0.74 -1.4 2,067,667 - rbp2 Synpcc7942_1999 0.76 -1.4 2,067,667 - rbp2 Synpcc7942_1999 0.76 -1.7 2,067,800 - -2.8 2,067,845 + -3.3 2,067,935 + -1.6 2,067,966 + -1.9 2,067,966 + -0.9 2,067,966 + -0.6 2,067,966 + -3.0 2,067,974 - +0.2 2,067,974 - +1.0 2,067,994 - -2.2 2,068,122 - -1.7 2,068,398 + mrcB Synpcc7942_2000 0.20 -4.3 2,068,629 - mrcB Synpcc7942_2000 0.30 -2.1
Or see this region's nucleotide sequence