Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pys and Synpcc7942_1985 are separated by 32 nucleotides Synpcc7942_1985 and pqqL overlap by 8 nucleotides pqqL and rpaC are separated by 165 nucleotides
Synpcc7942_1984: pys - phytoene synthase, at 2,054,486 to 2,055,412
pys
Synpcc7942_1985: Synpcc7942_1985 - hypothetical protein, at 2,055,445 to 2,056,338
_1985
Synpcc7942_1986: pqqL - processing protease, at 2,056,331 to 2,057,653
pqqL
Synpcc7942_1987: rpaC - hypothetical protein, at 2,057,819 to 2,058,133
rpaC
Position (kb)
2056
2057
2058 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2055.488 kb on - strand at 2055.493 kb on - strand at 2055.501 kb on - strand at 2055.547 kb on - strand, within Synpcc7942_1985 at 2055.633 kb on - strand, within Synpcc7942_1985 at 2055.670 kb on + strand, within Synpcc7942_1985 at 2055.745 kb on + strand, within Synpcc7942_1985 at 2055.755 kb on + strand, within Synpcc7942_1985 at 2055.763 kb on - strand, within Synpcc7942_1985 at 2055.763 kb on - strand, within Synpcc7942_1985 at 2055.763 kb on - strand, within Synpcc7942_1985 at 2055.801 kb on + strand, within Synpcc7942_1985 at 2055.801 kb on + strand, within Synpcc7942_1985 at 2055.801 kb on + strand, within Synpcc7942_1985 at 2055.801 kb on + strand, within Synpcc7942_1985 at 2055.809 kb on - strand, within Synpcc7942_1985 at 2055.809 kb on - strand, within Synpcc7942_1985 at 2055.816 kb on - strand, within Synpcc7942_1985 at 2055.827 kb on + strand at 2055.839 kb on + strand, within Synpcc7942_1985 at 2055.839 kb on + strand, within Synpcc7942_1985 at 2055.892 kb on - strand, within Synpcc7942_1985 at 2055.952 kb on + strand, within Synpcc7942_1985 at 2055.952 kb on + strand, within Synpcc7942_1985 at 2055.960 kb on - strand, within Synpcc7942_1985 at 2055.960 kb on - strand, within Synpcc7942_1985 at 2055.960 kb on - strand, within Synpcc7942_1985 at 2055.960 kb on - strand, within Synpcc7942_1985 at 2055.961 kb on - strand, within Synpcc7942_1985 at 2055.970 kb on - strand, within Synpcc7942_1985 at 2055.975 kb on - strand, within Synpcc7942_1985 at 2056.005 kb on + strand, within Synpcc7942_1985 at 2056.040 kb on - strand, within Synpcc7942_1985 at 2056.042 kb on + strand, within Synpcc7942_1985 at 2056.106 kb on - strand, within Synpcc7942_1985 at 2056.122 kb on - strand, within Synpcc7942_1985 at 2056.131 kb on + strand, within Synpcc7942_1985 at 2056.131 kb on + strand, within Synpcc7942_1985 at 2056.191 kb on + strand, within Synpcc7942_1985 at 2056.202 kb on + strand, within Synpcc7942_1985 at 2056.215 kb on + strand, within Synpcc7942_1985 at 2056.237 kb on + strand, within Synpcc7942_1985 at 2056.238 kb on + strand, within Synpcc7942_1985 at 2056.243 kb on + strand, within Synpcc7942_1985 at 2056.250 kb on + strand at 2056.276 kb on - strand at 2056.289 kb on + strand at 2056.289 kb on + strand at 2056.297 kb on - strand at 2056.297 kb on - strand at 2056.297 kb on - strand at 2056.297 kb on - strand at 2056.299 kb on - strand at 2056.383 kb on + strand at 2056.391 kb on - strand at 2056.391 kb on - strand at 2056.470 kb on - strand, within pqqL at 2056.474 kb on - strand, within pqqL at 2056.474 kb on - strand, within pqqL at 2056.487 kb on - strand, within pqqL at 2056.538 kb on + strand, within pqqL at 2056.570 kb on - strand, within pqqL at 2056.578 kb on - strand, within pqqL at 2056.578 kb on - strand, within pqqL at 2056.592 kb on - strand, within pqqL at 2056.607 kb on - strand, within pqqL at 2056.632 kb on + strand, within pqqL at 2056.640 kb on - strand, within pqqL at 2056.642 kb on + strand, within pqqL at 2056.652 kb on - strand, within pqqL at 2056.698 kb on - strand, within pqqL at 2056.724 kb on + strand, within pqqL at 2056.724 kb on + strand, within pqqL at 2056.732 kb on - strand, within pqqL at 2056.734 kb on + strand, within pqqL at 2056.735 kb on - strand, within pqqL at 2056.761 kb on - strand, within pqqL at 2056.780 kb on - strand, within pqqL at 2056.796 kb on - strand, within pqqL at 2056.800 kb on + strand, within pqqL at 2056.800 kb on + strand, within pqqL at 2056.806 kb on + strand, within pqqL at 2056.806 kb on + strand, within pqqL at 2056.808 kb on - strand, within pqqL at 2056.842 kb on + strand, within pqqL at 2056.848 kb on - strand, within pqqL at 2056.867 kb on + strand, within pqqL at 2056.875 kb on - strand, within pqqL at 2056.920 kb on - strand, within pqqL at 2056.926 kb on + strand, within pqqL at 2056.934 kb on - strand, within pqqL at 2056.936 kb on + strand, within pqqL at 2056.949 kb on - strand, within pqqL at 2056.971 kb on - strand, within pqqL at 2057.004 kb on + strand, within pqqL at 2057.009 kb on + strand, within pqqL at 2057.012 kb on - strand, within pqqL at 2057.033 kb on - strand, within pqqL at 2057.085 kb on - strand, within pqqL at 2057.101 kb on + strand, within pqqL at 2057.115 kb on - strand, within pqqL at 2057.144 kb on - strand, within pqqL at 2057.145 kb on - strand, within pqqL at 2057.150 kb on - strand, within pqqL at 2057.150 kb on - strand, within pqqL at 2057.294 kb on + strand, within pqqL at 2057.310 kb on - strand, within pqqL at 2057.310 kb on - strand, within pqqL at 2057.321 kb on - strand, within pqqL at 2057.325 kb on + strand, within pqqL at 2057.333 kb on - strand, within pqqL at 2057.338 kb on - strand, within pqqL at 2057.338 kb on - strand, within pqqL at 2057.346 kb on + strand, within pqqL at 2057.387 kb on - strand, within pqqL at 2057.420 kb on - strand, within pqqL at 2057.465 kb on - strand, within pqqL at 2057.494 kb on + strand, within pqqL at 2057.502 kb on - strand, within pqqL at 2057.502 kb on - strand, within pqqL at 2057.571 kb on + strand at 2057.625 kb on - strand at 2057.634 kb on - strand at 2057.639 kb on + strand at 2057.666 kb on - strand at 2057.687 kb on + strand at 2057.710 kb on + strand at 2057.742 kb on - strand at 2057.824 kb on - strand at 2057.919 kb on - strand, within rpaC at 2057.924 kb on + strand, within rpaC at 2057.932 kb on - strand, within rpaC at 2057.938 kb on - strand, within rpaC at 2057.940 kb on + strand, within rpaC at 2057.948 kb on - strand, within rpaC at 2057.948 kb on - strand, within rpaC at 2057.976 kb on + strand, within rpaC at 2058.039 kb on + strand, within rpaC at 2058.044 kb on - strand, within rpaC at 2058.172 kb on + strand at 2058.179 kb on - strand at 2058.200 kb on - strand at 2058.247 kb on + strand at 2058.277 kb on + strand at 2058.304 kb on - strand at 2058.368 kb on + strand at 2058.403 kb on + strand at 2058.420 kb on - strand at 2058.422 kb on + strand at 2058.456 kb on - strand at 2058.472 kb on - strand at 2058.472 kb on - strand at 2058.479 kb on - strand at 2058.486 kb on - strand at 2058.488 kb on + strand at 2058.496 kb on + strand at 2058.501 kb on + strand at 2058.504 kb on - strand at 2058.518 kb on + strand at 2058.610 kb on - strand at 2058.638 kb on + strand at 2058.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,055,488 - +3.2 2,055,493 - -0.6 2,055,501 - +0.6 2,055,547 - Synpcc7942_1985 0.11 -0.4 2,055,633 - Synpcc7942_1985 0.21 -1.3 2,055,670 + Synpcc7942_1985 0.25 -0.5 2,055,745 + Synpcc7942_1985 0.34 +1.8 2,055,755 + Synpcc7942_1985 0.35 +0.5 2,055,763 - Synpcc7942_1985 0.36 +0.6 2,055,763 - Synpcc7942_1985 0.36 +0.9 2,055,763 - Synpcc7942_1985 0.36 -0.8 2,055,801 + Synpcc7942_1985 0.40 +0.8 2,055,801 + Synpcc7942_1985 0.40 -3.5 2,055,801 + Synpcc7942_1985 0.40 +0.6 2,055,801 + Synpcc7942_1985 0.40 -1.1 2,055,809 - Synpcc7942_1985 0.41 -0.3 2,055,809 - Synpcc7942_1985 0.41 +0.5 2,055,816 - Synpcc7942_1985 0.41 +1.4 2,055,827 + -0.6 2,055,839 + Synpcc7942_1985 0.44 -1.3 2,055,839 + Synpcc7942_1985 0.44 -1.1 2,055,892 - Synpcc7942_1985 0.50 -3.3 2,055,952 + Synpcc7942_1985 0.57 +1.1 2,055,952 + Synpcc7942_1985 0.57 -1.7 2,055,960 - Synpcc7942_1985 0.58 -0.1 2,055,960 - Synpcc7942_1985 0.58 -0.7 2,055,960 - Synpcc7942_1985 0.58 +2.2 2,055,960 - Synpcc7942_1985 0.58 -0.4 2,055,961 - Synpcc7942_1985 0.58 -0.4 2,055,970 - Synpcc7942_1985 0.59 +4.6 2,055,975 - Synpcc7942_1985 0.59 -2.2 2,056,005 + Synpcc7942_1985 0.63 -0.4 2,056,040 - Synpcc7942_1985 0.67 -0.3 2,056,042 + Synpcc7942_1985 0.67 -1.2 2,056,106 - Synpcc7942_1985 0.74 +3.0 2,056,122 - Synpcc7942_1985 0.76 +0.8 2,056,131 + Synpcc7942_1985 0.77 +1.0 2,056,131 + Synpcc7942_1985 0.77 +0.2 2,056,191 + Synpcc7942_1985 0.83 +0.9 2,056,202 + Synpcc7942_1985 0.85 +0.9 2,056,215 + Synpcc7942_1985 0.86 +1.2 2,056,237 + Synpcc7942_1985 0.89 +3.3 2,056,238 + Synpcc7942_1985 0.89 -1.7 2,056,243 + Synpcc7942_1985 0.89 -2.1 2,056,250 + -0.7 2,056,276 - -1.5 2,056,289 + -1.1 2,056,289 + -2.3 2,056,297 - -0.4 2,056,297 - +3.7 2,056,297 - +0.8 2,056,297 - -1.8 2,056,299 - -2.7 2,056,383 + -2.8 2,056,391 - -2.1 2,056,391 - -2.1 2,056,470 - pqqL Synpcc7942_1986 0.11 -0.4 2,056,474 - pqqL Synpcc7942_1986 0.11 -1.0 2,056,474 - pqqL Synpcc7942_1986 0.11 -2.6 2,056,487 - pqqL Synpcc7942_1986 0.12 -0.1 2,056,538 + pqqL Synpcc7942_1986 0.16 -1.2 2,056,570 - pqqL Synpcc7942_1986 0.18 -1.1 2,056,578 - pqqL Synpcc7942_1986 0.19 -1.6 2,056,578 - pqqL Synpcc7942_1986 0.19 +2.0 2,056,592 - pqqL Synpcc7942_1986 0.20 -1.4 2,056,607 - pqqL Synpcc7942_1986 0.21 -1.1 2,056,632 + pqqL Synpcc7942_1986 0.23 -1.1 2,056,640 - pqqL Synpcc7942_1986 0.23 -3.7 2,056,642 + pqqL Synpcc7942_1986 0.24 -3.2 2,056,652 - pqqL Synpcc7942_1986 0.24 -3.0 2,056,698 - pqqL Synpcc7942_1986 0.28 +0.4 2,056,724 + pqqL Synpcc7942_1986 0.30 -1.8 2,056,724 + pqqL Synpcc7942_1986 0.30 -1.4 2,056,732 - pqqL Synpcc7942_1986 0.30 -4.3 2,056,734 + pqqL Synpcc7942_1986 0.30 -0.1 2,056,735 - pqqL Synpcc7942_1986 0.31 -2.0 2,056,761 - pqqL Synpcc7942_1986 0.33 -0.8 2,056,780 - pqqL Synpcc7942_1986 0.34 -2.1 2,056,796 - pqqL Synpcc7942_1986 0.35 -1.0 2,056,800 + pqqL Synpcc7942_1986 0.35 -0.5 2,056,800 + pqqL Synpcc7942_1986 0.35 -1.4 2,056,806 + pqqL Synpcc7942_1986 0.36 -3.3 2,056,806 + pqqL Synpcc7942_1986 0.36 -1.8 2,056,808 - pqqL Synpcc7942_1986 0.36 -2.6 2,056,842 + pqqL Synpcc7942_1986 0.39 -3.4 2,056,848 - pqqL Synpcc7942_1986 0.39 -1.6 2,056,867 + pqqL Synpcc7942_1986 0.41 -1.0 2,056,875 - pqqL Synpcc7942_1986 0.41 +0.7 2,056,920 - pqqL Synpcc7942_1986 0.45 -2.8 2,056,926 + pqqL Synpcc7942_1986 0.45 -0.8 2,056,934 - pqqL Synpcc7942_1986 0.46 -2.1 2,056,936 + pqqL Synpcc7942_1986 0.46 -3.5 2,056,949 - pqqL Synpcc7942_1986 0.47 -3.8 2,056,971 - pqqL Synpcc7942_1986 0.48 -3.1 2,057,004 + pqqL Synpcc7942_1986 0.51 -1.0 2,057,009 + pqqL Synpcc7942_1986 0.51 -1.1 2,057,012 - pqqL Synpcc7942_1986 0.51 -2.6 2,057,033 - pqqL Synpcc7942_1986 0.53 -0.9 2,057,085 - pqqL Synpcc7942_1986 0.57 -2.6 2,057,101 + pqqL Synpcc7942_1986 0.58 -2.8 2,057,115 - pqqL Synpcc7942_1986 0.59 -1.3 2,057,144 - pqqL Synpcc7942_1986 0.61 -2.0 2,057,145 - pqqL Synpcc7942_1986 0.62 -2.3 2,057,150 - pqqL Synpcc7942_1986 0.62 -0.3 2,057,150 - pqqL Synpcc7942_1986 0.62 +1.2 2,057,294 + pqqL Synpcc7942_1986 0.73 -2.4 2,057,310 - pqqL Synpcc7942_1986 0.74 -3.9 2,057,310 - pqqL Synpcc7942_1986 0.74 -3.1 2,057,321 - pqqL Synpcc7942_1986 0.75 -3.0 2,057,325 + pqqL Synpcc7942_1986 0.75 +0.2 2,057,333 - pqqL Synpcc7942_1986 0.76 -3.3 2,057,338 - pqqL Synpcc7942_1986 0.76 -1.1 2,057,338 - pqqL Synpcc7942_1986 0.76 -1.4 2,057,346 + pqqL Synpcc7942_1986 0.77 -0.7 2,057,387 - pqqL Synpcc7942_1986 0.80 -3.1 2,057,420 - pqqL Synpcc7942_1986 0.82 -2.7 2,057,465 - pqqL Synpcc7942_1986 0.86 -1.3 2,057,494 + pqqL Synpcc7942_1986 0.88 -3.0 2,057,502 - pqqL Synpcc7942_1986 0.89 -4.1 2,057,502 - pqqL Synpcc7942_1986 0.89 -0.8 2,057,571 + -2.1 2,057,625 - -2.8 2,057,634 - +2.6 2,057,639 + -1.9 2,057,666 - -2.7 2,057,687 + -1.4 2,057,710 + -1.8 2,057,742 - +0.8 2,057,824 - -2.3 2,057,919 - rpaC Synpcc7942_1987 0.32 -1.2 2,057,924 + rpaC Synpcc7942_1987 0.33 -0.7 2,057,932 - rpaC Synpcc7942_1987 0.36 -0.6 2,057,938 - rpaC Synpcc7942_1987 0.38 +1.0 2,057,940 + rpaC Synpcc7942_1987 0.38 -0.6 2,057,948 - rpaC Synpcc7942_1987 0.41 -1.1 2,057,948 - rpaC Synpcc7942_1987 0.41 +0.6 2,057,976 + rpaC Synpcc7942_1987 0.50 +0.5 2,058,039 + rpaC Synpcc7942_1987 0.70 +2.2 2,058,044 - rpaC Synpcc7942_1987 0.71 +0.0 2,058,172 + -3.0 2,058,179 - +2.3 2,058,200 - -1.4 2,058,247 + +0.1 2,058,277 + -2.1 2,058,304 - +2.0 2,058,368 + -2.0 2,058,403 + +2.2 2,058,420 - +1.6 2,058,422 + +0.1 2,058,456 - +1.6 2,058,472 - +1.7 2,058,472 - +1.8 2,058,479 - -2.1 2,058,486 - +0.9 2,058,488 + +0.5 2,058,496 + -1.6 2,058,501 + -1.3 2,058,504 - -1.3 2,058,518 + -2.6 2,058,610 - +1.3 2,058,638 + +0.3 2,058,644 - +2.7
Or see this region's nucleotide sequence