Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt accD and Synpcc7942_1957 are separated by 120 nucleotides Synpcc7942_1957 and Synpcc7942_1958 are separated by 90 nucleotides Synpcc7942_1958 and proS are separated by 206 nucleotides
Synpcc7942_1956: accD - acetyl-CoA carboxylase subunit beta, at 2,028,196 to 2,029,113
accD
Synpcc7942_1957: Synpcc7942_1957 - cyclic nucleotide-binding domain (cNMP-BD) protein, at 2,029,234 to 2,029,671
_1957
Synpcc7942_1958: Synpcc7942_1958 - hypothetical protein, at 2,029,762 to 2,030,133
_1958
Synpcc7942_1959: proS - prolyl-tRNA synthetase, at 2,030,340 to 2,032,142
proS
Position (kb)
2029
2030
2031 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2029.037 kb on + strand at 2029.044 kb on - strand at 2029.107 kb on + strand at 2029.107 kb on + strand at 2029.126 kb on + strand at 2029.134 kb on - strand at 2029.139 kb on + strand at 2029.141 kb on + strand at 2029.156 kb on + strand at 2029.156 kb on + strand at 2029.164 kb on - strand at 2029.168 kb on + strand at 2029.242 kb on - strand at 2029.253 kb on - strand at 2029.255 kb on + strand at 2029.279 kb on + strand, within Synpcc7942_1957 at 2029.280 kb on - strand, within Synpcc7942_1957 at 2029.341 kb on - strand, within Synpcc7942_1957 at 2029.348 kb on + strand, within Synpcc7942_1957 at 2029.348 kb on + strand, within Synpcc7942_1957 at 2029.348 kb on + strand, within Synpcc7942_1957 at 2029.348 kb on + strand, within Synpcc7942_1957 at 2029.348 kb on + strand, within Synpcc7942_1957 at 2029.469 kb on - strand, within Synpcc7942_1957 at 2029.510 kb on - strand, within Synpcc7942_1957 at 2029.512 kb on - strand, within Synpcc7942_1957 at 2029.512 kb on - strand, within Synpcc7942_1957 at 2029.556 kb on + strand, within Synpcc7942_1957 at 2029.639 kb on - strand at 2029.646 kb on + strand at 2029.666 kb on + strand at 2029.685 kb on + strand at 2029.685 kb on + strand at 2029.685 kb on + strand at 2029.685 kb on + strand at 2029.685 kb on + strand at 2029.685 kb on + strand at 2029.693 kb on - strand at 2029.693 kb on - strand at 2029.693 kb on - strand at 2029.710 kb on - strand at 2029.720 kb on - strand at 2029.745 kb on - strand at 2029.746 kb on - strand at 2029.759 kb on + strand at 2029.759 kb on + strand at 2029.790 kb on - strand at 2029.848 kb on + strand, within Synpcc7942_1958 at 2029.859 kb on + strand, within Synpcc7942_1958 at 2029.897 kb on + strand, within Synpcc7942_1958 at 2029.902 kb on - strand, within Synpcc7942_1958 at 2029.937 kb on - strand, within Synpcc7942_1958 at 2030.030 kb on - strand, within Synpcc7942_1958 at 2030.030 kb on - strand, within Synpcc7942_1958 at 2030.032 kb on + strand, within Synpcc7942_1958 at 2030.044 kb on + strand, within Synpcc7942_1958 at 2030.044 kb on + strand, within Synpcc7942_1958 at 2030.055 kb on + strand, within Synpcc7942_1958 at 2030.072 kb on - strand, within Synpcc7942_1958 at 2030.139 kb on + strand at 2030.139 kb on + strand at 2030.139 kb on + strand at 2030.139 kb on + strand at 2030.158 kb on + strand at 2030.166 kb on - strand at 2030.216 kb on + strand at 2030.232 kb on + strand at 2030.233 kb on - strand at 2030.245 kb on + strand at 2030.250 kb on + strand at 2030.258 kb on - strand at 2030.258 kb on - strand at 2030.279 kb on - strand at 2030.287 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,029,037 + +1.1 2,029,044 - -1.0 2,029,107 + -2.1 2,029,107 + +3.7 2,029,126 + -0.7 2,029,134 - -0.9 2,029,139 + +2.5 2,029,141 + -0.1 2,029,156 + +1.9 2,029,156 + +2.4 2,029,164 - +0.2 2,029,168 + +1.7 2,029,242 - +0.8 2,029,253 - +1.7 2,029,255 + -2.8 2,029,279 + Synpcc7942_1957 0.10 -1.3 2,029,280 - Synpcc7942_1957 0.11 -1.7 2,029,341 - Synpcc7942_1957 0.24 -1.3 2,029,348 + Synpcc7942_1957 0.26 +2.1 2,029,348 + Synpcc7942_1957 0.26 +1.3 2,029,348 + Synpcc7942_1957 0.26 +1.0 2,029,348 + Synpcc7942_1957 0.26 +0.6 2,029,348 + Synpcc7942_1957 0.26 +3.6 2,029,469 - Synpcc7942_1957 0.54 +0.8 2,029,510 - Synpcc7942_1957 0.63 +1.6 2,029,512 - Synpcc7942_1957 0.63 -2.1 2,029,512 - Synpcc7942_1957 0.63 -0.7 2,029,556 + Synpcc7942_1957 0.74 +1.0 2,029,639 - +0.2 2,029,646 + +1.9 2,029,666 + -1.6 2,029,685 + -1.6 2,029,685 + -3.3 2,029,685 + -0.4 2,029,685 + -1.1 2,029,685 + +1.0 2,029,685 + -3.1 2,029,693 - -2.3 2,029,693 - -2.7 2,029,693 - +0.9 2,029,710 - -0.7 2,029,720 - -0.5 2,029,745 - +1.9 2,029,746 - -2.0 2,029,759 + +0.2 2,029,759 + -2.0 2,029,790 - -1.3 2,029,848 + Synpcc7942_1958 0.23 +0.2 2,029,859 + Synpcc7942_1958 0.26 -3.3 2,029,897 + Synpcc7942_1958 0.36 -0.1 2,029,902 - Synpcc7942_1958 0.38 -1.7 2,029,937 - Synpcc7942_1958 0.47 -1.5 2,030,030 - Synpcc7942_1958 0.72 -1.7 2,030,030 - Synpcc7942_1958 0.72 -3.2 2,030,032 + Synpcc7942_1958 0.73 -3.3 2,030,044 + Synpcc7942_1958 0.76 -3.3 2,030,044 + Synpcc7942_1958 0.76 -1.7 2,030,055 + Synpcc7942_1958 0.79 -2.1 2,030,072 - Synpcc7942_1958 0.83 -1.1 2,030,139 + -2.8 2,030,139 + -0.8 2,030,139 + -1.8 2,030,139 + +0.8 2,030,158 + -0.3 2,030,166 - -1.2 2,030,216 + +0.1 2,030,232 + -1.9 2,030,233 - -1.0 2,030,245 + +1.4 2,030,250 + +2.6 2,030,258 - -2.1 2,030,258 - -0.5 2,030,279 - +1.6 2,030,287 - -1.4
Or see this region's nucleotide sequence