Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and Synpcc7942_1946 are separated by 63 nucleotides Synpcc7942_1946 and Synpcc7942_1947 are separated by 116 nucleotides Synpcc7942_1947 and Synpcc7942_1948 are separated by 153 nucleotides Synpcc7942_1948 and Synpcc7942_1949 are separated by 63 nucleotides Synpcc7942_1949 and Synpcc7942_1950 are separated by 76 nucleotides
Synpcc7942_1945: uvrC - excinuclease ABC subunit C, at 2,019,498 to 2,021,429
uvrC
Synpcc7942_1946: Synpcc7942_1946 - hypothetical protein, at 2,021,493 to 2,021,972
_1946
Synpcc7942_1947: Synpcc7942_1947 - hypothetical protein, at 2,022,089 to 2,023,540
_1947
Synpcc7942_1948: Synpcc7942_1948 - hypothetical protein, at 2,023,694 to 2,023,939
_1948
Synpcc7942_1949: Synpcc7942_1949 - hypothetical protein, at 2,024,003 to 2,024,311
_1949
Synpcc7942_1950: Synpcc7942_1950 - hypothetical protein, at 2,024,388 to 2,024,639
_1950
Position (kb)
2022
2023
2024 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6 at 2021.115 kb on - strand, within uvrC at 2021.200 kb on - strand, within uvrC at 2021.397 kb on + strand at 2021.400 kb on + strand at 2021.483 kb on + strand at 2021.483 kb on + strand at 2021.490 kb on + strand at 2021.491 kb on - strand at 2021.496 kb on - strand at 2021.498 kb on - strand at 2021.584 kb on + strand, within Synpcc7942_1946 at 2021.633 kb on - strand, within Synpcc7942_1946 at 2021.641 kb on + strand, within Synpcc7942_1946 at 2021.644 kb on - strand, within Synpcc7942_1946 at 2021.644 kb on - strand, within Synpcc7942_1946 at 2021.649 kb on - strand, within Synpcc7942_1946 at 2021.700 kb on - strand, within Synpcc7942_1946 at 2021.714 kb on - strand, within Synpcc7942_1946 at 2021.761 kb on + strand, within Synpcc7942_1946 at 2021.769 kb on - strand, within Synpcc7942_1946 at 2021.786 kb on + strand, within Synpcc7942_1946 at 2021.793 kb on - strand, within Synpcc7942_1946 at 2021.794 kb on - strand, within Synpcc7942_1946 at 2021.794 kb on - strand, within Synpcc7942_1946 at 2021.816 kb on + strand, within Synpcc7942_1946 at 2021.824 kb on - strand at 2021.835 kb on + strand, within Synpcc7942_1946 at 2021.871 kb on - strand, within Synpcc7942_1946 at 2021.872 kb on - strand, within Synpcc7942_1946 at 2021.890 kb on + strand, within Synpcc7942_1946 at 2021.909 kb on + strand, within Synpcc7942_1946 at 2021.919 kb on + strand, within Synpcc7942_1946 at 2021.920 kb on + strand, within Synpcc7942_1946 at 2021.944 kb on - strand at 2021.968 kb on + strand at 2022.013 kb on + strand at 2022.013 kb on + strand at 2022.018 kb on + strand at 2022.021 kb on - strand at 2022.058 kb on - strand at 2022.090 kb on - strand at 2022.101 kb on - strand at 2022.137 kb on - strand at 2022.137 kb on - strand at 2022.137 kb on - strand at 2022.137 kb on - strand at 2022.137 kb on - strand at 2022.137 kb on - strand at 2022.137 kb on - strand at 2022.137 kb on - strand at 2022.137 kb on - strand at 2022.164 kb on - strand at 2022.177 kb on + strand at 2022.177 kb on + strand at 2022.203 kb on - strand at 2022.209 kb on + strand at 2022.251 kb on - strand, within Synpcc7942_1947 at 2022.251 kb on - strand, within Synpcc7942_1947 at 2022.295 kb on - strand, within Synpcc7942_1947 at 2022.298 kb on + strand, within Synpcc7942_1947 at 2022.300 kb on - strand, within Synpcc7942_1947 at 2022.345 kb on + strand, within Synpcc7942_1947 at 2022.367 kb on + strand, within Synpcc7942_1947 at 2022.369 kb on - strand, within Synpcc7942_1947 at 2022.426 kb on - strand, within Synpcc7942_1947 at 2022.454 kb on - strand, within Synpcc7942_1947 at 2022.461 kb on - strand, within Synpcc7942_1947 at 2022.469 kb on + strand, within Synpcc7942_1947 at 2022.492 kb on - strand, within Synpcc7942_1947 at 2022.509 kb on - strand, within Synpcc7942_1947 at 2022.560 kb on - strand, within Synpcc7942_1947 at 2022.589 kb on - strand, within Synpcc7942_1947 at 2022.589 kb on - strand, within Synpcc7942_1947 at 2022.589 kb on - strand, within Synpcc7942_1947 at 2022.627 kb on - strand, within Synpcc7942_1947 at 2022.643 kb on + strand, within Synpcc7942_1947 at 2022.643 kb on + strand, within Synpcc7942_1947 at 2022.653 kb on + strand, within Synpcc7942_1947 at 2022.654 kb on + strand, within Synpcc7942_1947 at 2022.656 kb on - strand, within Synpcc7942_1947 at 2022.686 kb on + strand, within Synpcc7942_1947 at 2022.694 kb on - strand, within Synpcc7942_1947 at 2022.694 kb on - strand, within Synpcc7942_1947 at 2022.694 kb on - strand, within Synpcc7942_1947 at 2022.694 kb on - strand, within Synpcc7942_1947 at 2022.724 kb on + strand, within Synpcc7942_1947 at 2022.724 kb on + strand, within Synpcc7942_1947 at 2022.732 kb on - strand, within Synpcc7942_1947 at 2022.871 kb on - strand, within Synpcc7942_1947 at 2022.904 kb on + strand, within Synpcc7942_1947 at 2022.945 kb on - strand, within Synpcc7942_1947 at 2022.948 kb on + strand, within Synpcc7942_1947 at 2022.950 kb on + strand, within Synpcc7942_1947 at 2023.092 kb on + strand, within Synpcc7942_1947 at 2023.092 kb on + strand, within Synpcc7942_1947 at 2023.100 kb on - strand, within Synpcc7942_1947 at 2023.100 kb on - strand, within Synpcc7942_1947 at 2023.100 kb on - strand, within Synpcc7942_1947 at 2023.169 kb on - strand, within Synpcc7942_1947 at 2023.183 kb on + strand, within Synpcc7942_1947 at 2023.191 kb on - strand, within Synpcc7942_1947 at 2023.226 kb on - strand, within Synpcc7942_1947 at 2023.234 kb on - strand, within Synpcc7942_1947 at 2023.241 kb on + strand, within Synpcc7942_1947 at 2023.241 kb on - strand, within Synpcc7942_1947 at 2023.283 kb on + strand, within Synpcc7942_1947 at 2023.283 kb on + strand, within Synpcc7942_1947 at 2023.306 kb on + strand, within Synpcc7942_1947 at 2023.309 kb on + strand, within Synpcc7942_1947 at 2023.366 kb on + strand, within Synpcc7942_1947 at 2023.367 kb on + strand, within Synpcc7942_1947 at 2023.374 kb on - strand, within Synpcc7942_1947 at 2023.374 kb on - strand, within Synpcc7942_1947 at 2023.375 kb on - strand, within Synpcc7942_1947 at 2023.405 kb on - strand at 2023.476 kb on - strand at 2023.481 kb on + strand at 2023.524 kb on - strand at 2023.566 kb on - strand at 2023.575 kb on + strand at 2023.580 kb on + strand at 2023.580 kb on + strand at 2023.580 kb on + strand at 2023.580 kb on + strand at 2023.580 kb on + strand at 2023.580 kb on + strand at 2023.580 kb on + strand at 2023.582 kb on + strand at 2023.588 kb on - strand at 2023.601 kb on - strand at 2023.601 kb on - strand at 2023.610 kb on + strand at 2023.649 kb on - strand at 2023.688 kb on + strand at 2023.729 kb on + strand, within Synpcc7942_1948 at 2023.777 kb on - strand, within Synpcc7942_1948 at 2023.800 kb on + strand, within Synpcc7942_1948 at 2023.962 kb on + strand at 2023.963 kb on - strand at 2023.963 kb on - strand at 2023.967 kb on + strand at 2023.967 kb on + strand at 2023.967 kb on + strand at 2023.967 kb on + strand at 2023.967 kb on + strand at 2023.967 kb on + strand at 2023.975 kb on - strand at 2023.975 kb on - strand at 2023.975 kb on - strand at 2023.975 kb on - strand at 2023.975 kb on - strand at 2023.975 kb on - strand at 2023.997 kb on - strand at 2024.000 kb on + strand at 2024.000 kb on + strand at 2024.008 kb on - strand at 2024.013 kb on - strand at 2024.022 kb on - strand at 2024.252 kb on + strand, within Synpcc7942_1949 at 2024.252 kb on + strand, within Synpcc7942_1949 at 2024.260 kb on - strand, within Synpcc7942_1949 at 2024.268 kb on - strand, within Synpcc7942_1949 at 2024.317 kb on + strand at 2024.326 kb on - strand at 2024.334 kb on - strand at 2024.373 kb on - strand at 2024.507 kb on - strand, within Synpcc7942_1950
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 2,021,115 - uvrC Synpcc7942_1945 0.84 -2.1 2,021,200 - uvrC Synpcc7942_1945 0.88 -2.7 2,021,397 + -1.3 2,021,400 + -2.0 2,021,483 + -3.7 2,021,483 + +1.1 2,021,490 + -0.9 2,021,491 - -2.5 2,021,496 - -2.4 2,021,498 - -3.2 2,021,584 + Synpcc7942_1946 0.19 +0.8 2,021,633 - Synpcc7942_1946 0.29 -1.4 2,021,641 + Synpcc7942_1946 0.31 -1.5 2,021,644 - Synpcc7942_1946 0.31 -0.7 2,021,644 - Synpcc7942_1946 0.31 +6.3 2,021,649 - Synpcc7942_1946 0.33 -2.4 2,021,700 - Synpcc7942_1946 0.43 -0.1 2,021,714 - Synpcc7942_1946 0.46 +0.3 2,021,761 + Synpcc7942_1946 0.56 -0.7 2,021,769 - Synpcc7942_1946 0.57 -0.5 2,021,786 + Synpcc7942_1946 0.61 +1.5 2,021,793 - Synpcc7942_1946 0.62 -0.4 2,021,794 - Synpcc7942_1946 0.63 -0.9 2,021,794 - Synpcc7942_1946 0.63 -1.5 2,021,816 + Synpcc7942_1946 0.67 +1.0 2,021,824 - -0.5 2,021,835 + Synpcc7942_1946 0.71 -1.1 2,021,871 - Synpcc7942_1946 0.79 +0.5 2,021,872 - Synpcc7942_1946 0.79 +1.7 2,021,890 + Synpcc7942_1946 0.83 -1.8 2,021,909 + Synpcc7942_1946 0.87 +0.0 2,021,919 + Synpcc7942_1946 0.89 +0.4 2,021,920 + Synpcc7942_1946 0.89 -1.4 2,021,944 - +0.8 2,021,968 + +0.1 2,022,013 + -2.3 2,022,013 + -1.3 2,022,018 + +2.6 2,022,021 - +0.0 2,022,058 - -0.7 2,022,090 - -3.7 2,022,101 - -1.1 2,022,137 - -2.0 2,022,137 - -2.8 2,022,137 - -2.6 2,022,137 - -3.3 2,022,137 - -3.8 2,022,137 - -1.8 2,022,137 - -3.4 2,022,137 - -5.1 2,022,137 - -3.1 2,022,164 - -4.1 2,022,177 + -1.4 2,022,177 + -0.9 2,022,203 - -1.6 2,022,209 + -3.3 2,022,251 - Synpcc7942_1947 0.11 -2.6 2,022,251 - Synpcc7942_1947 0.11 +0.8 2,022,295 - Synpcc7942_1947 0.14 -1.3 2,022,298 + Synpcc7942_1947 0.14 -4.1 2,022,300 - Synpcc7942_1947 0.15 +0.1 2,022,345 + Synpcc7942_1947 0.18 -3.5 2,022,367 + Synpcc7942_1947 0.19 -2.1 2,022,369 - Synpcc7942_1947 0.19 -4.6 2,022,426 - Synpcc7942_1947 0.23 -2.5 2,022,454 - Synpcc7942_1947 0.25 -2.1 2,022,461 - Synpcc7942_1947 0.26 -2.3 2,022,469 + Synpcc7942_1947 0.26 +1.4 2,022,492 - Synpcc7942_1947 0.28 -4.2 2,022,509 - Synpcc7942_1947 0.29 -1.6 2,022,560 - Synpcc7942_1947 0.32 -2.3 2,022,589 - Synpcc7942_1947 0.34 -1.9 2,022,589 - Synpcc7942_1947 0.34 -4.5 2,022,589 - Synpcc7942_1947 0.34 -1.4 2,022,627 - Synpcc7942_1947 0.37 -2.6 2,022,643 + Synpcc7942_1947 0.38 -2.6 2,022,643 + Synpcc7942_1947 0.38 -5.7 2,022,653 + Synpcc7942_1947 0.39 -4.4 2,022,654 + Synpcc7942_1947 0.39 -2.7 2,022,656 - Synpcc7942_1947 0.39 -4.6 2,022,686 + Synpcc7942_1947 0.41 -2.5 2,022,694 - Synpcc7942_1947 0.42 -1.1 2,022,694 - Synpcc7942_1947 0.42 -2.8 2,022,694 - Synpcc7942_1947 0.42 -2.6 2,022,694 - Synpcc7942_1947 0.42 -1.3 2,022,724 + Synpcc7942_1947 0.44 -3.7 2,022,724 + Synpcc7942_1947 0.44 -2.8 2,022,732 - Synpcc7942_1947 0.44 -4.9 2,022,871 - Synpcc7942_1947 0.54 -1.5 2,022,904 + Synpcc7942_1947 0.56 -3.3 2,022,945 - Synpcc7942_1947 0.59 -1.4 2,022,948 + Synpcc7942_1947 0.59 -2.7 2,022,950 + Synpcc7942_1947 0.59 -2.0 2,023,092 + Synpcc7942_1947 0.69 -3.8 2,023,092 + Synpcc7942_1947 0.69 -2.2 2,023,100 - Synpcc7942_1947 0.70 -2.3 2,023,100 - Synpcc7942_1947 0.70 -4.0 2,023,100 - Synpcc7942_1947 0.70 -3.0 2,023,169 - Synpcc7942_1947 0.74 -4.6 2,023,183 + Synpcc7942_1947 0.75 -5.1 2,023,191 - Synpcc7942_1947 0.76 -3.7 2,023,226 - Synpcc7942_1947 0.78 -3.1 2,023,234 - Synpcc7942_1947 0.79 -3.6 2,023,241 + Synpcc7942_1947 0.79 -4.2 2,023,241 - Synpcc7942_1947 0.79 -5.6 2,023,283 + Synpcc7942_1947 0.82 -2.7 2,023,283 + Synpcc7942_1947 0.82 -1.8 2,023,306 + Synpcc7942_1947 0.84 -2.6 2,023,309 + Synpcc7942_1947 0.84 -3.6 2,023,366 + Synpcc7942_1947 0.88 -2.5 2,023,367 + Synpcc7942_1947 0.88 -1.1 2,023,374 - Synpcc7942_1947 0.88 -2.5 2,023,374 - Synpcc7942_1947 0.88 -2.1 2,023,375 - Synpcc7942_1947 0.89 -3.1 2,023,405 - -2.4 2,023,476 - -0.9 2,023,481 + -1.7 2,023,524 - -0.7 2,023,566 - -0.7 2,023,575 + +0.2 2,023,580 + +1.5 2,023,580 + +1.9 2,023,580 + +0.1 2,023,580 + +0.6 2,023,580 + -0.5 2,023,580 + -1.9 2,023,580 + -1.2 2,023,582 + -1.9 2,023,588 - -4.4 2,023,601 - -1.2 2,023,601 - -0.3 2,023,610 + +1.4 2,023,649 - +1.5 2,023,688 + -1.6 2,023,729 + Synpcc7942_1948 0.14 +0.5 2,023,777 - Synpcc7942_1948 0.34 -1.4 2,023,800 + Synpcc7942_1948 0.43 -1.7 2,023,962 + -0.1 2,023,963 - +0.8 2,023,963 - +1.4 2,023,967 + -1.1 2,023,967 + -2.6 2,023,967 + +0.3 2,023,967 + +3.4 2,023,967 + -0.9 2,023,967 + -1.1 2,023,975 - +4.3 2,023,975 - -0.4 2,023,975 - -1.4 2,023,975 - -1.2 2,023,975 - -1.9 2,023,975 - -2.3 2,023,997 - -1.3 2,024,000 + -0.9 2,024,000 + +3.2 2,024,008 - +0.5 2,024,013 - -0.0 2,024,022 - +0.5 2,024,252 + Synpcc7942_1949 0.81 -2.4 2,024,252 + Synpcc7942_1949 0.81 +0.1 2,024,260 - Synpcc7942_1949 0.83 +2.1 2,024,268 - Synpcc7942_1949 0.86 +0.6 2,024,317 + -1.7 2,024,326 - -0.7 2,024,334 - -1.1 2,024,373 - -1.6 2,024,507 - Synpcc7942_1950 0.47 +0.1
Or see this region's nucleotide sequence