Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recJ and Synpcc7942_1887 are separated by 52 nucleotides Synpcc7942_1887 and Synpcc7942_1888 are separated by 34 nucleotides
Synpcc7942_1886: recJ - exonuclease RecJ, at 1,957,417 to 1,959,471
recJ
Synpcc7942_1887: Synpcc7942_1887 - probable mannosyltransferase, at 1,959,524 to 1,960,816
_1887
Synpcc7942_1888: Synpcc7942_1888 - hypothetical protein, at 1,960,851 to 1,961,891
_1888
Position (kb)
1959
1960
1961 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1958.536 kb on - strand at 1958.556 kb on + strand, within recJ at 1958.594 kb on + strand, within recJ at 1958.604 kb on - strand, within recJ at 1958.614 kb on + strand, within recJ at 1958.749 kb on + strand, within recJ at 1958.753 kb on + strand at 1958.772 kb on - strand, within recJ at 1958.821 kb on - strand, within recJ at 1958.920 kb on - strand, within recJ at 1959.054 kb on + strand, within recJ at 1959.054 kb on + strand at 1959.130 kb on - strand, within recJ at 1959.244 kb on - strand, within recJ at 1959.348 kb on + strand at 1959.420 kb on - strand at 1959.539 kb on + strand at 1960.623 kb on + strand, within Synpcc7942_1887 at 1960.930 kb on - strand at 1960.978 kb on + strand, within Synpcc7942_1888 at 1961.016 kb on + strand at 1961.049 kb on - strand, within Synpcc7942_1888 at 1961.059 kb on - strand, within Synpcc7942_1888 at 1961.066 kb on - strand, within Synpcc7942_1888 at 1961.117 kb on + strand, within Synpcc7942_1888 at 1961.117 kb on + strand, within Synpcc7942_1888 at 1961.117 kb on + strand, within Synpcc7942_1888 at 1961.125 kb on + strand, within Synpcc7942_1888 at 1961.125 kb on - strand, within Synpcc7942_1888 at 1961.125 kb on - strand, within Synpcc7942_1888 at 1961.125 kb on - strand, within Synpcc7942_1888 at 1961.125 kb on - strand, within Synpcc7942_1888 at 1961.125 kb on - strand, within Synpcc7942_1888 at 1961.125 kb on - strand, within Synpcc7942_1888 at 1961.125 kb on - strand, within Synpcc7942_1888 at 1961.125 kb on - strand, within Synpcc7942_1888 at 1961.125 kb on - strand, within Synpcc7942_1888 at 1961.207 kb on - strand, within Synpcc7942_1888 at 1961.270 kb on + strand, within Synpcc7942_1888 at 1961.410 kb on + strand, within Synpcc7942_1888 at 1961.473 kb on + strand, within Synpcc7942_1888 at 1961.517 kb on + strand, within Synpcc7942_1888 at 1961.525 kb on - strand, within Synpcc7942_1888 at 1961.575 kb on - strand, within Synpcc7942_1888 at 1961.632 kb on + strand, within Synpcc7942_1888 at 1961.692 kb on - strand, within Synpcc7942_1888 at 1961.692 kb on - strand, within Synpcc7942_1888 at 1961.692 kb on - strand, within Synpcc7942_1888 at 1961.692 kb on - strand, within Synpcc7942_1888 at 1961.714 kb on + strand, within Synpcc7942_1888 at 1961.714 kb on + strand, within Synpcc7942_1888 at 1961.714 kb on + strand, within Synpcc7942_1888 at 1961.714 kb on + strand, within Synpcc7942_1888 at 1961.722 kb on - strand, within Synpcc7942_1888 at 1961.733 kb on - strand, within Synpcc7942_1888 at 1961.758 kb on + strand, within Synpcc7942_1888 at 1961.808 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,958,536 - -2.5 1,958,556 + recJ Synpcc7942_1886 0.55 -0.1 1,958,594 + recJ Synpcc7942_1886 0.57 -1.7 1,958,604 - recJ Synpcc7942_1886 0.58 -3.2 1,958,614 + recJ Synpcc7942_1886 0.58 -2.0 1,958,749 + recJ Synpcc7942_1886 0.65 -1.9 1,958,753 + -1.9 1,958,772 - recJ Synpcc7942_1886 0.66 -2.9 1,958,821 - recJ Synpcc7942_1886 0.68 +2.7 1,958,920 - recJ Synpcc7942_1886 0.73 +0.6 1,959,054 + recJ Synpcc7942_1886 0.80 -2.7 1,959,054 + -1.3 1,959,130 - recJ Synpcc7942_1886 0.83 -1.2 1,959,244 - recJ Synpcc7942_1886 0.89 +0.2 1,959,348 + -1.9 1,959,420 - -0.3 1,959,539 + -1.7 1,960,623 + Synpcc7942_1887 0.85 -3.8 1,960,930 - -2.3 1,960,978 + Synpcc7942_1888 0.12 -0.7 1,961,016 + -1.6 1,961,049 - Synpcc7942_1888 0.19 -0.6 1,961,059 - Synpcc7942_1888 0.20 -1.5 1,961,066 - Synpcc7942_1888 0.21 +0.1 1,961,117 + Synpcc7942_1888 0.26 -0.5 1,961,117 + Synpcc7942_1888 0.26 -0.9 1,961,117 + Synpcc7942_1888 0.26 +0.2 1,961,125 + Synpcc7942_1888 0.26 +1.1 1,961,125 - Synpcc7942_1888 0.26 -3.7 1,961,125 - Synpcc7942_1888 0.26 +3.4 1,961,125 - Synpcc7942_1888 0.26 -2.0 1,961,125 - Synpcc7942_1888 0.26 -2.2 1,961,125 - Synpcc7942_1888 0.26 +0.8 1,961,125 - Synpcc7942_1888 0.26 +0.3 1,961,125 - Synpcc7942_1888 0.26 +1.9 1,961,125 - Synpcc7942_1888 0.26 +0.9 1,961,125 - Synpcc7942_1888 0.26 -1.5 1,961,207 - Synpcc7942_1888 0.34 -3.0 1,961,270 + Synpcc7942_1888 0.40 -1.9 1,961,410 + Synpcc7942_1888 0.54 -0.2 1,961,473 + Synpcc7942_1888 0.60 -0.0 1,961,517 + Synpcc7942_1888 0.64 +1.9 1,961,525 - Synpcc7942_1888 0.65 -3.6 1,961,575 - Synpcc7942_1888 0.70 +0.0 1,961,632 + Synpcc7942_1888 0.75 -2.9 1,961,692 - Synpcc7942_1888 0.81 +2.2 1,961,692 - Synpcc7942_1888 0.81 +0.8 1,961,692 - Synpcc7942_1888 0.81 +2.4 1,961,692 - Synpcc7942_1888 0.81 +2.3 1,961,714 + Synpcc7942_1888 0.83 -1.3 1,961,714 + Synpcc7942_1888 0.83 +1.2 1,961,714 + Synpcc7942_1888 0.83 +1.0 1,961,714 + Synpcc7942_1888 0.83 -2.9 1,961,722 - Synpcc7942_1888 0.84 +1.4 1,961,733 - Synpcc7942_1888 0.85 +1.4 1,961,758 + Synpcc7942_1888 0.87 +0.9 1,961,808 + +3.8
Or see this region's nucleotide sequence