Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moxR and Synpcc7942_1880 are separated by 231 nucleotides Synpcc7942_1880 and nadB are separated by 48 nucleotides nadB and psbU are separated by 87 nucleotides psbU and ycf53 are separated by 182 nucleotides
Synpcc7942_1879: moxR - MoxR protein-like, at 1,951,649 to 1,952,587
moxR
Synpcc7942_1880: Synpcc7942_1880 - hypothetical protein, at 1,952,819 to 1,953,025
_1880
Synpcc7942_1881: nadB - L-aspartate oxidase, at 1,953,074 to 1,954,726
nadB
Synpcc7942_1882: psbU - photosystem II complex extrinsic protein precursor PsuB, at 1,954,814 to 1,955,224
psbU
Synpcc7942_1883: ycf53 - conserved hypothetical protein YCF53, at 1,955,407 to 1,956,126
ycf53
Position (kb)
1953
1954
1955 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1952.091 kb on + strand, within moxR at 1952.109 kb on - strand, within moxR at 1952.180 kb on - strand, within moxR at 1952.187 kb on - strand, within moxR at 1952.208 kb on + strand, within moxR at 1952.208 kb on + strand, within moxR at 1952.210 kb on + strand, within moxR at 1952.216 kb on - strand, within moxR at 1952.397 kb on + strand, within moxR at 1952.410 kb on - strand, within moxR at 1952.480 kb on + strand, within moxR at 1952.533 kb on - strand at 1952.542 kb on - strand at 1952.542 kb on - strand at 1952.546 kb on + strand at 1952.568 kb on + strand at 1952.579 kb on + strand at 1952.592 kb on - strand at 1952.592 kb on - strand at 1952.592 kb on - strand at 1952.594 kb on + strand at 1952.599 kb on + strand at 1952.602 kb on - strand at 1952.624 kb on - strand at 1952.630 kb on + strand at 1952.637 kb on - strand at 1952.654 kb on + strand at 1952.662 kb on - strand at 1952.668 kb on - strand at 1952.685 kb on - strand at 1952.712 kb on + strand at 1952.773 kb on + strand at 1952.781 kb on - strand at 1952.797 kb on + strand at 1952.807 kb on + strand at 1952.809 kb on + strand at 1952.809 kb on + strand at 1952.856 kb on + strand, within Synpcc7942_1880 at 1952.898 kb on + strand, within Synpcc7942_1880 at 1952.898 kb on + strand, within Synpcc7942_1880 at 1952.902 kb on + strand at 1952.999 kb on + strand, within Synpcc7942_1880 at 1953.006 kb on + strand at 1953.007 kb on - strand at 1953.098 kb on - strand at 1953.108 kb on + strand at 1953.127 kb on - strand at 1953.172 kb on - strand at 1953.234 kb on - strand at 1953.303 kb on - strand, within nadB at 1953.376 kb on - strand, within nadB at 1953.376 kb on - strand, within nadB at 1953.376 kb on - strand, within nadB at 1953.431 kb on - strand, within nadB at 1953.613 kb on - strand, within nadB at 1953.632 kb on - strand, within nadB at 1953.644 kb on - strand, within nadB at 1953.651 kb on - strand, within nadB at 1953.670 kb on + strand, within nadB at 1953.675 kb on + strand, within nadB at 1953.678 kb on - strand, within nadB at 1953.698 kb on + strand, within nadB at 1953.698 kb on - strand, within nadB at 1953.819 kb on + strand, within nadB at 1953.827 kb on - strand, within nadB at 1953.829 kb on + strand, within nadB at 1953.859 kb on + strand, within nadB at 1953.959 kb on + strand, within nadB at 1953.965 kb on + strand, within nadB at 1953.999 kb on + strand, within nadB at 1954.012 kb on - strand, within nadB at 1954.185 kb on - strand, within nadB at 1954.203 kb on + strand, within nadB at 1954.216 kb on - strand, within nadB at 1954.290 kb on - strand, within nadB at 1954.407 kb on + strand, within nadB at 1954.421 kb on - strand, within nadB at 1954.444 kb on - strand, within nadB at 1954.483 kb on + strand, within nadB at 1954.495 kb on + strand, within nadB at 1954.502 kb on - strand, within nadB at 1954.503 kb on - strand, within nadB at 1954.571 kb on + strand at 1954.596 kb on + strand at 1954.639 kb on + strand at 1954.641 kb on + strand at 1954.684 kb on - strand at 1954.722 kb on + strand at 1954.722 kb on + strand at 1954.730 kb on - strand at 1954.791 kb on + strand at 1954.799 kb on + strand at 1954.813 kb on - strand at 1954.818 kb on - strand at 1954.891 kb on + strand, within psbU at 1954.898 kb on - strand, within psbU at 1954.898 kb on - strand, within psbU at 1954.958 kb on + strand, within psbU at 1954.967 kb on - strand, within psbU at 1954.994 kb on + strand, within psbU at 1955.001 kb on - strand, within psbU at 1955.003 kb on - strand, within psbU at 1955.023 kb on - strand, within psbU at 1955.040 kb on - strand, within psbU at 1955.052 kb on + strand, within psbU at 1955.140 kb on + strand, within psbU at 1955.145 kb on + strand, within psbU at 1955.228 kb on + strand at 1955.236 kb on - strand at 1955.317 kb on + strand at 1955.325 kb on - strand at 1955.327 kb on - strand at 1955.334 kb on + strand at 1955.342 kb on + strand at 1955.342 kb on + strand at 1955.342 kb on - strand at 1955.342 kb on - strand at 1955.356 kb on - strand at 1955.361 kb on + strand at 1955.393 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,952,091 + moxR Synpcc7942_1879 0.47 +0.0 1,952,109 - moxR Synpcc7942_1879 0.49 +3.5 1,952,180 - moxR Synpcc7942_1879 0.57 -1.2 1,952,187 - moxR Synpcc7942_1879 0.57 +2.4 1,952,208 + moxR Synpcc7942_1879 0.60 +4.6 1,952,208 + moxR Synpcc7942_1879 0.60 +0.1 1,952,210 + moxR Synpcc7942_1879 0.60 -1.2 1,952,216 - moxR Synpcc7942_1879 0.60 +4.0 1,952,397 + moxR Synpcc7942_1879 0.80 +1.1 1,952,410 - moxR Synpcc7942_1879 0.81 +3.1 1,952,480 + moxR Synpcc7942_1879 0.88 +0.8 1,952,533 - +4.4 1,952,542 - +1.5 1,952,542 - +0.6 1,952,546 + -2.3 1,952,568 + +2.3 1,952,579 + +1.0 1,952,592 - +0.7 1,952,592 - +2.6 1,952,592 - +1.8 1,952,594 + -0.4 1,952,599 + +2.7 1,952,602 - +1.6 1,952,624 - +2.4 1,952,630 + +1.5 1,952,637 - -1.4 1,952,654 + +0.3 1,952,662 - +4.7 1,952,668 - -0.3 1,952,685 - -0.4 1,952,712 + -0.3 1,952,773 + +0.1 1,952,781 - +0.1 1,952,797 + +5.7 1,952,807 + -2.5 1,952,809 + +0.5 1,952,809 + -1.9 1,952,856 + Synpcc7942_1880 0.18 -2.3 1,952,898 + Synpcc7942_1880 0.38 -1.6 1,952,898 + Synpcc7942_1880 0.38 -2.9 1,952,902 + +3.9 1,952,999 + Synpcc7942_1880 0.87 -3.2 1,953,006 + -0.6 1,953,007 - -0.6 1,953,098 - -0.7 1,953,108 + -1.1 1,953,127 - -3.4 1,953,172 - +1.4 1,953,234 - +5.9 1,953,303 - nadB Synpcc7942_1881 0.14 +1.1 1,953,376 - nadB Synpcc7942_1881 0.18 -2.7 1,953,376 - nadB Synpcc7942_1881 0.18 -3.0 1,953,376 - nadB Synpcc7942_1881 0.18 -1.1 1,953,431 - nadB Synpcc7942_1881 0.22 -2.9 1,953,613 - nadB Synpcc7942_1881 0.33 -0.1 1,953,632 - nadB Synpcc7942_1881 0.34 -2.8 1,953,644 - nadB Synpcc7942_1881 0.34 -2.0 1,953,651 - nadB Synpcc7942_1881 0.35 -0.3 1,953,670 + nadB Synpcc7942_1881 0.36 -0.1 1,953,675 + nadB Synpcc7942_1881 0.36 -2.2 1,953,678 - nadB Synpcc7942_1881 0.37 -0.3 1,953,698 + nadB Synpcc7942_1881 0.38 -1.3 1,953,698 - nadB Synpcc7942_1881 0.38 -1.6 1,953,819 + nadB Synpcc7942_1881 0.45 -1.3 1,953,827 - nadB Synpcc7942_1881 0.46 -1.7 1,953,829 + nadB Synpcc7942_1881 0.46 +1.5 1,953,859 + nadB Synpcc7942_1881 0.47 -2.8 1,953,959 + nadB Synpcc7942_1881 0.54 -0.8 1,953,965 + nadB Synpcc7942_1881 0.54 -0.9 1,953,999 + nadB Synpcc7942_1881 0.56 +0.5 1,954,012 - nadB Synpcc7942_1881 0.57 -1.1 1,954,185 - nadB Synpcc7942_1881 0.67 -2.8 1,954,203 + nadB Synpcc7942_1881 0.68 -1.8 1,954,216 - nadB Synpcc7942_1881 0.69 +0.1 1,954,290 - nadB Synpcc7942_1881 0.74 -0.5 1,954,407 + nadB Synpcc7942_1881 0.81 -0.8 1,954,421 - nadB Synpcc7942_1881 0.81 -0.9 1,954,444 - nadB Synpcc7942_1881 0.83 -1.9 1,954,483 + nadB Synpcc7942_1881 0.85 -1.5 1,954,495 + nadB Synpcc7942_1881 0.86 -1.3 1,954,502 - nadB Synpcc7942_1881 0.86 +1.6 1,954,503 - nadB Synpcc7942_1881 0.86 -0.9 1,954,571 + -1.5 1,954,596 + -3.6 1,954,639 + -0.7 1,954,641 + -0.5 1,954,684 - -0.5 1,954,722 + +1.9 1,954,722 + -1.6 1,954,730 - +2.1 1,954,791 + +3.8 1,954,799 + +0.9 1,954,813 - -1.8 1,954,818 - +2.5 1,954,891 + psbU Synpcc7942_1882 0.19 +1.3 1,954,898 - psbU Synpcc7942_1882 0.20 +0.4 1,954,898 - psbU Synpcc7942_1882 0.20 +0.8 1,954,958 + psbU Synpcc7942_1882 0.35 +0.7 1,954,967 - psbU Synpcc7942_1882 0.37 +0.8 1,954,994 + psbU Synpcc7942_1882 0.44 -0.7 1,955,001 - psbU Synpcc7942_1882 0.45 +0.8 1,955,003 - psbU Synpcc7942_1882 0.46 +0.1 1,955,023 - psbU Synpcc7942_1882 0.51 -0.1 1,955,040 - psbU Synpcc7942_1882 0.55 -2.5 1,955,052 + psbU Synpcc7942_1882 0.58 -0.7 1,955,140 + psbU Synpcc7942_1882 0.79 -0.3 1,955,145 + psbU Synpcc7942_1882 0.81 -1.3 1,955,228 + +4.1 1,955,236 - -1.1 1,955,317 + +2.4 1,955,325 - -1.2 1,955,327 - +1.6 1,955,334 + -3.5 1,955,342 + -1.1 1,955,342 + +3.1 1,955,342 - +1.3 1,955,342 - +2.4 1,955,356 - -1.7 1,955,361 + +0.3 1,955,393 - +1.1
Or see this region's nucleotide sequence