Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hetI and prxQ-A2 are separated by 14 nucleotides prxQ-A2 and mutT are separated by 55 nucleotides mutT and Synpcc7942_1808 overlap by 28 nucleotides Synpcc7942_1808 and dfa1 are separated by 65 nucleotides
Synpcc7942_1805: hetI - HetI protein-like, at 1,874,474 to 1,875,253
hetI
Synpcc7942_1806: prxQ-A2 - bacterioferritin comigratory protein, at 1,875,268 to 1,875,741
prxQ-A2
Synpcc7942_1807: mutT - mutator MutT-like, at 1,875,797 to 1,876,243
mutT
Synpcc7942_1808: Synpcc7942_1808 - pantothenate kinase, at 1,876,216 to 1,876,959
_1808
Synpcc7942_1809: dfa1 - flavoprotein, at 1,877,025 to 1,878,740
dfa1
Position (kb)
1875
1876
1877 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1875.171 kb on + strand, within hetI at 1875.171 kb on + strand, within hetI at 1875.177 kb on + strand at 1875.177 kb on + strand at 1875.179 kb on + strand at 1875.185 kb on - strand at 1875.218 kb on + strand at 1875.231 kb on + strand at 1875.237 kb on + strand at 1875.238 kb on + strand at 1875.239 kb on + strand at 1875.257 kb on + strand at 1875.257 kb on + strand at 1875.258 kb on - strand at 1875.280 kb on - strand at 1875.321 kb on + strand, within prxQ-A2 at 1875.329 kb on + strand, within prxQ-A2 at 1875.338 kb on + strand, within prxQ-A2 at 1875.353 kb on - strand at 1875.361 kb on - strand, within prxQ-A2 at 1875.372 kb on - strand, within prxQ-A2 at 1875.404 kb on + strand, within prxQ-A2 at 1875.409 kb on - strand, within prxQ-A2 at 1875.463 kb on + strand, within prxQ-A2 at 1875.464 kb on + strand, within prxQ-A2 at 1875.472 kb on - strand, within prxQ-A2 at 1875.472 kb on - strand, within prxQ-A2 at 1875.476 kb on + strand, within prxQ-A2 at 1875.499 kb on - strand, within prxQ-A2 at 1875.504 kb on - strand, within prxQ-A2 at 1875.525 kb on + strand, within prxQ-A2 at 1875.533 kb on - strand, within prxQ-A2 at 1875.545 kb on - strand, within prxQ-A2 at 1875.607 kb on + strand, within prxQ-A2 at 1875.617 kb on - strand, within prxQ-A2 at 1875.617 kb on - strand, within prxQ-A2 at 1875.651 kb on + strand, within prxQ-A2 at 1875.666 kb on + strand, within prxQ-A2 at 1875.666 kb on + strand at 1875.674 kb on - strand, within prxQ-A2 at 1875.686 kb on - strand, within prxQ-A2 at 1875.715 kb on + strand at 1875.715 kb on + strand at 1875.723 kb on - strand at 1875.742 kb on + strand at 1875.773 kb on + strand at 1875.773 kb on + strand at 1875.773 kb on + strand at 1875.773 kb on - strand at 1875.778 kb on + strand at 1875.787 kb on + strand at 1875.808 kb on + strand at 1875.808 kb on + strand at 1875.808 kb on + strand at 1875.816 kb on - strand at 1875.832 kb on + strand at 1875.840 kb on - strand at 1875.840 kb on - strand at 1875.897 kb on - strand, within mutT at 1875.897 kb on - strand, within mutT at 1875.916 kb on + strand, within mutT at 1875.916 kb on + strand, within mutT at 1875.916 kb on + strand, within mutT at 1875.916 kb on + strand, within mutT at 1875.916 kb on + strand, within mutT at 1875.928 kb on + strand, within mutT at 1875.936 kb on - strand, within mutT at 1875.936 kb on - strand, within mutT at 1876.014 kb on + strand, within mutT at 1876.027 kb on + strand, within mutT at 1876.033 kb on + strand, within mutT at 1876.033 kb on + strand, within mutT at 1876.033 kb on + strand, within mutT at 1876.033 kb on + strand, within mutT at 1876.041 kb on - strand, within mutT at 1876.041 kb on - strand, within mutT at 1876.041 kb on - strand, within mutT at 1876.041 kb on - strand, within mutT at 1876.054 kb on - strand, within mutT at 1876.085 kb on - strand, within mutT at 1876.133 kb on - strand, within mutT at 1876.141 kb on - strand, within mutT at 1876.150 kb on + strand, within mutT at 1876.173 kb on - strand, within mutT at 1876.177 kb on + strand, within mutT at 1876.177 kb on + strand, within mutT at 1876.179 kb on - strand, within mutT at 1876.185 kb on + strand, within mutT at 1876.185 kb on - strand, within mutT at 1876.185 kb on - strand at 1876.193 kb on - strand, within mutT at 1876.193 kb on - strand, within mutT at 1876.205 kb on + strand at 1876.213 kb on - strand at 1876.213 kb on - strand at 1876.213 kb on - strand at 1876.237 kb on - strand at 1876.947 kb on - strand at 1876.947 kb on - strand at 1876.970 kb on - strand at 1876.981 kb on + strand at 1877.058 kb on - strand at 1877.152 kb on - strand at 1877.159 kb on + strand at 1877.159 kb on + strand at 1877.165 kb on - strand at 1877.206 kb on - strand, within dfa1 at 1877.219 kb on + strand, within dfa1 at 1877.233 kb on + strand, within dfa1 at 1877.233 kb on + strand, within dfa1
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,875,171 + hetI Synpcc7942_1805 0.89 -2.6 1,875,171 + hetI Synpcc7942_1805 0.89 -0.1 1,875,177 + -1.0 1,875,177 + -0.0 1,875,179 + +0.3 1,875,185 - -1.6 1,875,218 + +0.6 1,875,231 + -1.4 1,875,237 + +0.2 1,875,238 + -3.1 1,875,239 + -0.4 1,875,257 + -1.3 1,875,257 + +2.8 1,875,258 - +1.6 1,875,280 - +1.3 1,875,321 + prxQ-A2 Synpcc7942_1806 0.11 -0.8 1,875,329 + prxQ-A2 Synpcc7942_1806 0.13 +3.6 1,875,338 + prxQ-A2 Synpcc7942_1806 0.15 +0.5 1,875,353 - -1.5 1,875,361 - prxQ-A2 Synpcc7942_1806 0.20 +0.9 1,875,372 - prxQ-A2 Synpcc7942_1806 0.22 +1.5 1,875,404 + prxQ-A2 Synpcc7942_1806 0.29 +1.0 1,875,409 - prxQ-A2 Synpcc7942_1806 0.30 -0.3 1,875,463 + prxQ-A2 Synpcc7942_1806 0.41 +0.5 1,875,464 + prxQ-A2 Synpcc7942_1806 0.41 -1.8 1,875,472 - prxQ-A2 Synpcc7942_1806 0.43 +2.5 1,875,472 - prxQ-A2 Synpcc7942_1806 0.43 -1.5 1,875,476 + prxQ-A2 Synpcc7942_1806 0.44 +0.8 1,875,499 - prxQ-A2 Synpcc7942_1806 0.49 -1.6 1,875,504 - prxQ-A2 Synpcc7942_1806 0.50 -1.2 1,875,525 + prxQ-A2 Synpcc7942_1806 0.54 +3.7 1,875,533 - prxQ-A2 Synpcc7942_1806 0.56 +1.3 1,875,545 - prxQ-A2 Synpcc7942_1806 0.58 +1.6 1,875,607 + prxQ-A2 Synpcc7942_1806 0.72 -1.1 1,875,617 - prxQ-A2 Synpcc7942_1806 0.74 +1.7 1,875,617 - prxQ-A2 Synpcc7942_1806 0.74 -1.5 1,875,651 + prxQ-A2 Synpcc7942_1806 0.81 -0.9 1,875,666 + prxQ-A2 Synpcc7942_1806 0.84 +0.1 1,875,666 + +0.1 1,875,674 - prxQ-A2 Synpcc7942_1806 0.86 +2.2 1,875,686 - prxQ-A2 Synpcc7942_1806 0.88 -0.1 1,875,715 + +3.1 1,875,715 + +0.3 1,875,723 - +0.1 1,875,742 + -0.7 1,875,773 + -1.1 1,875,773 + +1.1 1,875,773 + -1.0 1,875,773 - +2.1 1,875,778 + +0.6 1,875,787 + -2.2 1,875,808 + -0.9 1,875,808 + +1.8 1,875,808 + +0.1 1,875,816 - +1.3 1,875,832 + -2.0 1,875,840 - -2.7 1,875,840 - -1.1 1,875,897 - mutT Synpcc7942_1807 0.22 -2.2 1,875,897 - mutT Synpcc7942_1807 0.22 -0.3 1,875,916 + mutT Synpcc7942_1807 0.27 -0.5 1,875,916 + mutT Synpcc7942_1807 0.27 -2.7 1,875,916 + mutT Synpcc7942_1807 0.27 -3.1 1,875,916 + mutT Synpcc7942_1807 0.27 -0.9 1,875,916 + mutT Synpcc7942_1807 0.27 -1.7 1,875,928 + mutT Synpcc7942_1807 0.29 -2.6 1,875,936 - mutT Synpcc7942_1807 0.31 -2.4 1,875,936 - mutT Synpcc7942_1807 0.31 -2.1 1,876,014 + mutT Synpcc7942_1807 0.49 -0.9 1,876,027 + mutT Synpcc7942_1807 0.51 -3.9 1,876,033 + mutT Synpcc7942_1807 0.53 -2.1 1,876,033 + mutT Synpcc7942_1807 0.53 -2.4 1,876,033 + mutT Synpcc7942_1807 0.53 -1.5 1,876,033 + mutT Synpcc7942_1807 0.53 +0.2 1,876,041 - mutT Synpcc7942_1807 0.55 -1.7 1,876,041 - mutT Synpcc7942_1807 0.55 -0.7 1,876,041 - mutT Synpcc7942_1807 0.55 -0.5 1,876,041 - mutT Synpcc7942_1807 0.55 -0.5 1,876,054 - mutT Synpcc7942_1807 0.57 -0.7 1,876,085 - mutT Synpcc7942_1807 0.64 -1.6 1,876,133 - mutT Synpcc7942_1807 0.75 -0.8 1,876,141 - mutT Synpcc7942_1807 0.77 -0.7 1,876,150 + mutT Synpcc7942_1807 0.79 -1.5 1,876,173 - mutT Synpcc7942_1807 0.84 -2.4 1,876,177 + mutT Synpcc7942_1807 0.85 +1.4 1,876,177 + mutT Synpcc7942_1807 0.85 -0.1 1,876,179 - mutT Synpcc7942_1807 0.85 +0.5 1,876,185 + mutT Synpcc7942_1807 0.87 -1.1 1,876,185 - mutT Synpcc7942_1807 0.87 -3.2 1,876,185 - +0.1 1,876,193 - mutT Synpcc7942_1807 0.89 -2.3 1,876,193 - mutT Synpcc7942_1807 0.89 +1.5 1,876,205 + -0.7 1,876,213 - -2.0 1,876,213 - -1.5 1,876,213 - -1.4 1,876,237 - +1.5 1,876,947 - +1.8 1,876,947 - +2.1 1,876,970 - +2.7 1,876,981 + -1.5 1,877,058 - -1.5 1,877,152 - +4.5 1,877,159 + +2.7 1,877,159 + -1.3 1,877,165 - -0.7 1,877,206 - dfa1 Synpcc7942_1809 0.11 +2.3 1,877,219 + dfa1 Synpcc7942_1809 0.11 -1.4 1,877,233 + dfa1 Synpcc7942_1809 0.12 +3.6 1,877,233 + dfa1 Synpcc7942_1809 0.12 -0.9
Or see this region's nucleotide sequence