Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1783 and sigF2 are separated by 358 nucleotides sigF2 and Synpcc7942_1785 overlap by 4 nucleotides Synpcc7942_1785 and ycf51 are separated by 4 nucleotides ycf51 and nifU overlap by 4 nucleotides
Synpcc7942_1783: Synpcc7942_1783 - hypothetical protein, at 1,849,303 to 1,852,170
_1783
Synpcc7942_1784: sigF2 - RNA polymerase sigma factor SigF, at 1,852,529 to 1,853,311
sigF2
Synpcc7942_1785: Synpcc7942_1785 - hypothetical protein, at 1,853,308 to 1,853,718
_1785
Synpcc7942_1786: ycf51 - conserved hypothetical protein YCF51, at 1,853,723 to 1,854,250
ycf51
Synpcc7942_1787: nifU - SUF system FeS assembly protein, at 1,854,247 to 1,854,729
nifU
Position (kb)
1852
1853
1854 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 1851.533 kb on - strand, within Synpcc7942_1783 at 1851.533 kb on - strand, within Synpcc7942_1783 at 1851.536 kb on - strand, within Synpcc7942_1783 at 1851.536 kb on - strand, within Synpcc7942_1783 at 1851.536 kb on - strand, within Synpcc7942_1783 at 1851.558 kb on - strand, within Synpcc7942_1783 at 1851.572 kb on + strand, within Synpcc7942_1783 at 1851.572 kb on + strand, within Synpcc7942_1783 at 1851.572 kb on + strand, within Synpcc7942_1783 at 1851.572 kb on + strand, within Synpcc7942_1783 at 1851.580 kb on - strand, within Synpcc7942_1783 at 1851.598 kb on + strand, within Synpcc7942_1783 at 1851.606 kb on + strand, within Synpcc7942_1783 at 1851.608 kb on - strand, within Synpcc7942_1783 at 1851.614 kb on - strand, within Synpcc7942_1783 at 1851.636 kb on + strand, within Synpcc7942_1783 at 1851.644 kb on - strand, within Synpcc7942_1783 at 1851.672 kb on + strand, within Synpcc7942_1783 at 1851.712 kb on - strand, within Synpcc7942_1783 at 1851.722 kb on - strand, within Synpcc7942_1783 at 1851.722 kb on - strand, within Synpcc7942_1783 at 1851.783 kb on - strand, within Synpcc7942_1783 at 1851.803 kb on - strand, within Synpcc7942_1783 at 1851.844 kb on + strand, within Synpcc7942_1783 at 1851.901 kb on + strand at 1851.932 kb on - strand at 1851.954 kb on + strand at 1851.954 kb on + strand at 1851.954 kb on + strand at 1851.959 kb on + strand at 1851.959 kb on + strand at 1851.962 kb on - strand at 1851.962 kb on - strand at 1851.962 kb on - strand at 1851.962 kb on - strand at 1851.962 kb on - strand at 1851.962 kb on - strand at 1851.962 kb on - strand at 1851.962 kb on - strand at 1851.967 kb on + strand at 1851.967 kb on - strand at 1851.967 kb on - strand at 1851.982 kb on - strand at 1851.982 kb on - strand at 1851.982 kb on - strand at 1851.995 kb on - strand at 1852.016 kb on + strand at 1852.036 kb on - strand at 1852.057 kb on - strand at 1852.057 kb on - strand at 1852.059 kb on + strand at 1852.067 kb on - strand at 1852.086 kb on + strand at 1852.097 kb on - strand at 1852.108 kb on - strand at 1852.108 kb on - strand at 1852.123 kb on + strand at 1852.125 kb on + strand at 1852.133 kb on - strand at 1852.147 kb on + strand at 1852.147 kb on + strand at 1852.147 kb on + strand at 1852.149 kb on + strand at 1852.149 kb on + strand at 1852.154 kb on + strand at 1852.157 kb on - strand at 1852.159 kb on + strand at 1852.222 kb on + strand at 1852.224 kb on + strand at 1852.224 kb on + strand at 1852.225 kb on + strand at 1852.232 kb on - strand at 1852.232 kb on - strand at 1852.232 kb on - strand at 1852.267 kb on + strand at 1852.267 kb on + strand at 1852.268 kb on - strand at 1852.277 kb on + strand at 1852.321 kb on - strand at 1852.336 kb on - strand at 1852.367 kb on - strand at 1852.397 kb on + strand at 1852.405 kb on + strand at 1852.415 kb on + strand at 1852.415 kb on + strand at 1852.415 kb on + strand at 1852.417 kb on + strand at 1852.427 kb on + strand at 1852.427 kb on + strand at 1852.427 kb on + strand at 1852.435 kb on - strand at 1852.435 kb on - strand at 1852.435 kb on - strand at 1852.435 kb on - strand at 1852.471 kb on - strand at 1852.485 kb on - strand at 1852.511 kb on + strand at 1852.511 kb on + strand at 1852.511 kb on + strand at 1852.519 kb on - strand at 1852.519 kb on - strand at 1852.544 kb on + strand at 1852.560 kb on + strand at 1852.561 kb on + strand at 1852.609 kb on + strand, within sigF2 at 1852.629 kb on + strand, within sigF2 at 1852.629 kb on + strand, within sigF2 at 1852.674 kb on + strand, within sigF2 at 1852.700 kb on - strand, within sigF2 at 1852.723 kb on - strand, within sigF2 at 1852.738 kb on + strand, within sigF2 at 1852.748 kb on + strand, within sigF2 at 1852.756 kb on - strand, within sigF2 at 1852.756 kb on - strand, within sigF2 at 1852.780 kb on + strand, within sigF2 at 1852.794 kb on + strand, within sigF2 at 1852.830 kb on + strand, within sigF2 at 1852.864 kb on + strand, within sigF2 at 1852.874 kb on - strand, within sigF2 at 1852.897 kb on - strand, within sigF2 at 1852.909 kb on - strand, within sigF2 at 1852.941 kb on - strand at 1852.951 kb on + strand, within sigF2 at 1852.959 kb on - strand, within sigF2 at 1853.015 kb on - strand, within sigF2 at 1853.097 kb on + strand, within sigF2 at 1853.097 kb on + strand, within sigF2 at 1853.097 kb on + strand, within sigF2 at 1853.097 kb on + strand, within sigF2 at 1853.097 kb on + strand, within sigF2 at 1853.105 kb on - strand, within sigF2 at 1853.105 kb on - strand, within sigF2 at 1853.105 kb on - strand, within sigF2 at 1853.109 kb on + strand, within sigF2 at 1853.171 kb on + strand, within sigF2 at 1853.306 kb on + strand at 1853.306 kb on + strand at 1853.307 kb on - strand at 1853.309 kb on - strand at 1853.314 kb on - strand at 1853.314 kb on - strand at 1853.326 kb on + strand at 1853.358 kb on + strand, within Synpcc7942_1785 at 1853.400 kb on + strand, within Synpcc7942_1785 at 1853.410 kb on + strand, within Synpcc7942_1785 at 1853.434 kb on - strand, within Synpcc7942_1785 at 1853.462 kb on + strand at 1853.472 kb on - strand, within Synpcc7942_1785 at 1853.472 kb on - strand, within Synpcc7942_1785 at 1853.472 kb on - strand, within Synpcc7942_1785 at 1853.479 kb on - strand, within Synpcc7942_1785 at 1853.484 kb on - strand, within Synpcc7942_1785 at 1853.489 kb on + strand, within Synpcc7942_1785 at 1853.507 kb on + strand, within Synpcc7942_1785 at 1853.515 kb on - strand, within Synpcc7942_1785 at 1853.519 kb on + strand, within Synpcc7942_1785 at 1853.519 kb on + strand, within Synpcc7942_1785 at 1853.519 kb on + strand, within Synpcc7942_1785 at 1853.519 kb on + strand, within Synpcc7942_1785 at 1853.527 kb on - strand, within Synpcc7942_1785 at 1853.539 kb on - strand, within Synpcc7942_1785 at 1853.585 kb on - strand, within Synpcc7942_1785 at 1853.613 kb on + strand, within Synpcc7942_1785 at 1853.622 kb on + strand, within Synpcc7942_1785 at 1853.623 kb on + strand, within Synpcc7942_1785 at 1853.623 kb on + strand, within Synpcc7942_1785 at 1853.623 kb on + strand, within Synpcc7942_1785 at 1853.623 kb on + strand, within Synpcc7942_1785 at 1853.623 kb on + strand, within Synpcc7942_1785 at 1853.623 kb on + strand, within Synpcc7942_1785 at 1853.669 kb on + strand, within Synpcc7942_1785 at 1853.669 kb on + strand, within Synpcc7942_1785 at 1853.669 kb on + strand, within Synpcc7942_1785 at 1853.679 kb on + strand at 1853.686 kb on - strand at 1853.687 kb on - strand at 1853.689 kb on - strand at 1853.689 kb on - strand at 1853.699 kb on + strand at 1853.704 kb on + strand at 1853.704 kb on + strand at 1853.704 kb on + strand at 1853.704 kb on + strand at 1853.704 kb on + strand at 1853.704 kb on + strand at 1853.704 kb on + strand at 1853.704 kb on + strand at 1853.707 kb on - strand at 1853.712 kb on - strand at 1854.245 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,851,533 - Synpcc7942_1783 0.78 -0.9 1,851,533 - Synpcc7942_1783 0.78 +2.4 1,851,536 - Synpcc7942_1783 0.78 +1.4 1,851,536 - Synpcc7942_1783 0.78 -0.2 1,851,536 - Synpcc7942_1783 0.78 +0.4 1,851,558 - Synpcc7942_1783 0.79 -1.1 1,851,572 + Synpcc7942_1783 0.79 +0.2 1,851,572 + Synpcc7942_1783 0.79 -2.2 1,851,572 + Synpcc7942_1783 0.79 +1.7 1,851,572 + Synpcc7942_1783 0.79 +2.2 1,851,580 - Synpcc7942_1783 0.79 +0.1 1,851,598 + Synpcc7942_1783 0.80 -1.8 1,851,606 + Synpcc7942_1783 0.80 +1.9 1,851,608 - Synpcc7942_1783 0.80 -0.3 1,851,614 - Synpcc7942_1783 0.81 +1.9 1,851,636 + Synpcc7942_1783 0.81 -0.9 1,851,644 - Synpcc7942_1783 0.82 +1.8 1,851,672 + Synpcc7942_1783 0.83 +4.3 1,851,712 - Synpcc7942_1783 0.84 -1.1 1,851,722 - Synpcc7942_1783 0.84 +1.6 1,851,722 - Synpcc7942_1783 0.84 -0.6 1,851,783 - Synpcc7942_1783 0.86 -1.0 1,851,803 - Synpcc7942_1783 0.87 +2.1 1,851,844 + Synpcc7942_1783 0.89 -1.7 1,851,901 + -1.4 1,851,932 - +0.1 1,851,954 + -3.8 1,851,954 + -2.6 1,851,954 + -0.4 1,851,959 + -0.5 1,851,959 + -2.3 1,851,962 - +0.6 1,851,962 - -1.1 1,851,962 - +1.9 1,851,962 - +2.5 1,851,962 - -0.6 1,851,962 - -0.2 1,851,962 - -1.0 1,851,962 - -0.7 1,851,967 + -0.7 1,851,967 - -0.5 1,851,967 - -0.5 1,851,982 - +1.4 1,851,982 - -0.6 1,851,982 - -1.7 1,851,995 - -2.5 1,852,016 + -1.7 1,852,036 - +1.6 1,852,057 - -0.5 1,852,057 - +3.2 1,852,059 + +1.9 1,852,067 - -0.5 1,852,086 + -0.3 1,852,097 - -0.9 1,852,108 - +1.3 1,852,108 - -1.3 1,852,123 + -0.5 1,852,125 + +1.4 1,852,133 - +1.7 1,852,147 + +1.9 1,852,147 + -1.4 1,852,147 + +0.8 1,852,149 + -2.4 1,852,149 + -0.4 1,852,154 + +0.3 1,852,157 - +0.5 1,852,159 + -1.4 1,852,222 + +1.9 1,852,224 + +1.5 1,852,224 + -0.7 1,852,225 + +2.4 1,852,232 - +0.2 1,852,232 - +0.3 1,852,232 - +0.5 1,852,267 + -0.9 1,852,267 + -0.5 1,852,268 - +1.9 1,852,277 + -0.9 1,852,321 - +2.2 1,852,336 - +2.3 1,852,367 - +2.0 1,852,397 + +2.9 1,852,405 + +1.9 1,852,415 + +2.1 1,852,415 + +6.3 1,852,415 + +3.4 1,852,417 + +1.3 1,852,427 + +3.3 1,852,427 + +1.0 1,852,427 + +1.2 1,852,435 - +3.2 1,852,435 - +0.9 1,852,435 - +2.4 1,852,435 - +7.2 1,852,471 - +4.9 1,852,485 - -0.3 1,852,511 + +2.7 1,852,511 + +1.5 1,852,511 + +0.3 1,852,519 - +2.0 1,852,519 - +1.4 1,852,544 + +3.1 1,852,560 + +1.1 1,852,561 + +3.4 1,852,609 + sigF2 Synpcc7942_1784 0.10 +3.8 1,852,629 + sigF2 Synpcc7942_1784 0.13 +2.3 1,852,629 + sigF2 Synpcc7942_1784 0.13 +0.5 1,852,674 + sigF2 Synpcc7942_1784 0.19 +1.6 1,852,700 - sigF2 Synpcc7942_1784 0.22 +4.0 1,852,723 - sigF2 Synpcc7942_1784 0.25 +4.2 1,852,738 + sigF2 Synpcc7942_1784 0.27 -1.7 1,852,748 + sigF2 Synpcc7942_1784 0.28 +1.2 1,852,756 - sigF2 Synpcc7942_1784 0.29 +3.6 1,852,756 - sigF2 Synpcc7942_1784 0.29 +3.1 1,852,780 + sigF2 Synpcc7942_1784 0.32 -0.7 1,852,794 + sigF2 Synpcc7942_1784 0.34 +2.0 1,852,830 + sigF2 Synpcc7942_1784 0.38 +3.1 1,852,864 + sigF2 Synpcc7942_1784 0.43 -1.4 1,852,874 - sigF2 Synpcc7942_1784 0.44 -1.2 1,852,897 - sigF2 Synpcc7942_1784 0.47 +1.9 1,852,909 - sigF2 Synpcc7942_1784 0.49 +1.6 1,852,941 - +0.7 1,852,951 + sigF2 Synpcc7942_1784 0.54 +2.1 1,852,959 - sigF2 Synpcc7942_1784 0.55 +1.9 1,853,015 - sigF2 Synpcc7942_1784 0.62 +2.6 1,853,097 + sigF2 Synpcc7942_1784 0.73 +3.3 1,853,097 + sigF2 Synpcc7942_1784 0.73 -2.5 1,853,097 + sigF2 Synpcc7942_1784 0.73 +4.9 1,853,097 + sigF2 Synpcc7942_1784 0.73 +3.0 1,853,097 + sigF2 Synpcc7942_1784 0.73 +1.5 1,853,105 - sigF2 Synpcc7942_1784 0.74 +0.9 1,853,105 - sigF2 Synpcc7942_1784 0.74 +3.2 1,853,105 - sigF2 Synpcc7942_1784 0.74 +2.5 1,853,109 + sigF2 Synpcc7942_1784 0.74 +0.5 1,853,171 + sigF2 Synpcc7942_1784 0.82 +3.8 1,853,306 + +0.2 1,853,306 + +0.6 1,853,307 - -2.0 1,853,309 - -0.5 1,853,314 - +0.5 1,853,314 - +2.5 1,853,326 + -2.0 1,853,358 + Synpcc7942_1785 0.12 +2.7 1,853,400 + Synpcc7942_1785 0.22 +4.9 1,853,410 + Synpcc7942_1785 0.25 -1.2 1,853,434 - Synpcc7942_1785 0.31 +0.9 1,853,462 + -1.1 1,853,472 - Synpcc7942_1785 0.40 -1.6 1,853,472 - Synpcc7942_1785 0.40 -0.2 1,853,472 - Synpcc7942_1785 0.40 -1.2 1,853,479 - Synpcc7942_1785 0.42 -0.2 1,853,484 - Synpcc7942_1785 0.43 +0.7 1,853,489 + Synpcc7942_1785 0.44 -1.5 1,853,507 + Synpcc7942_1785 0.48 -0.7 1,853,515 - Synpcc7942_1785 0.50 +0.9 1,853,519 + Synpcc7942_1785 0.51 -0.4 1,853,519 + Synpcc7942_1785 0.51 -1.7 1,853,519 + Synpcc7942_1785 0.51 +0.5 1,853,519 + Synpcc7942_1785 0.51 -0.8 1,853,527 - Synpcc7942_1785 0.53 +1.8 1,853,539 - Synpcc7942_1785 0.56 +2.0 1,853,585 - Synpcc7942_1785 0.67 -0.8 1,853,613 + Synpcc7942_1785 0.74 -2.4 1,853,622 + Synpcc7942_1785 0.76 +1.5 1,853,623 + Synpcc7942_1785 0.77 -0.6 1,853,623 + Synpcc7942_1785 0.77 +0.9 1,853,623 + Synpcc7942_1785 0.77 -0.1 1,853,623 + Synpcc7942_1785 0.77 +1.9 1,853,623 + Synpcc7942_1785 0.77 +0.3 1,853,623 + Synpcc7942_1785 0.77 -2.1 1,853,669 + Synpcc7942_1785 0.88 -1.0 1,853,669 + Synpcc7942_1785 0.88 +1.8 1,853,669 + Synpcc7942_1785 0.88 -1.0 1,853,679 + -1.8 1,853,686 - +0.1 1,853,687 - -0.5 1,853,689 - -2.6 1,853,689 - -1.5 1,853,699 + +3.1 1,853,704 + +1.5 1,853,704 + +0.1 1,853,704 + +1.7 1,853,704 + -2.2 1,853,704 + +2.9 1,853,704 + -1.4 1,853,704 + -2.0 1,853,704 + +0.5 1,853,707 - -1.5 1,853,712 - +0.6 1,854,245 + +0.2
Or see this region's nucleotide sequence