Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fer and ycf35 overlap by 1 nucleotides ycf35 and Synpcc7942_1751 are separated by 42 nucleotides Synpcc7942_1751 and Synpcc7942_1752 are separated by 239 nucleotides Synpcc7942_1752 and Synpcc7942_1753 are separated by 119 nucleotides Synpcc7942_1753 and Synpcc7942_1754 overlap by 10 nucleotides Synpcc7942_1754 and Synpcc7942_1755 overlap by 4 nucleotides
Synpcc7942_1749: fer - ferredoxin, at 1,819,754 to 1,820,146
fer
Synpcc7942_1750: ycf35 - conserved hypothetical protein YCF35, at 1,820,146 to 1,820,532
ycf35
Synpcc7942_1751: Synpcc7942_1751 - hypothetical protein, at 1,820,575 to 1,820,778
_1751
Synpcc7942_1752: Synpcc7942_1752 - Armadillo:PBS lyase HEAT-like repeat, at 1,821,018 to 1,821,770
_1752
Synpcc7942_1753: Synpcc7942_1753 - hypothetical protein, at 1,821,890 to 1,822,354
_1753
Synpcc7942_1754: Synpcc7942_1754 - hypothetical protein, at 1,822,345 to 1,822,497
_1754
Synpcc7942_1755: Synpcc7942_1755 - hypothetical protein, at 1,822,494 to 1,824,227
_1755
Position (kb)
1821
1822 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 1820.020 kb on + strand, within fer at 1820.033 kb on - strand, within fer at 1820.047 kb on + strand, within fer at 1820.047 kb on + strand, within fer at 1820.055 kb on - strand, within fer at 1820.059 kb on + strand, within fer at 1820.059 kb on + strand, within fer at 1820.067 kb on - strand, within fer at 1820.142 kb on + strand at 1820.143 kb on + strand at 1820.143 kb on + strand at 1820.149 kb on - strand at 1820.201 kb on - strand, within ycf35 at 1820.218 kb on + strand, within ycf35 at 1820.218 kb on - strand, within ycf35 at 1820.227 kb on - strand, within ycf35 at 1820.236 kb on + strand, within ycf35 at 1820.244 kb on - strand, within ycf35 at 1820.249 kb on - strand, within ycf35 at 1820.263 kb on - strand at 1820.265 kb on + strand, within ycf35 at 1820.301 kb on - strand, within ycf35 at 1820.302 kb on - strand, within ycf35 at 1820.323 kb on + strand, within ycf35 at 1820.330 kb on + strand, within ycf35 at 1820.331 kb on + strand, within ycf35 at 1820.338 kb on - strand, within ycf35 at 1820.339 kb on - strand, within ycf35 at 1820.339 kb on - strand, within ycf35 at 1820.346 kb on - strand, within ycf35 at 1820.348 kb on + strand, within ycf35 at 1820.372 kb on + strand, within ycf35 at 1820.422 kb on - strand, within ycf35 at 1820.425 kb on - strand, within ycf35 at 1820.432 kb on + strand, within ycf35 at 1820.432 kb on + strand at 1820.432 kb on + strand, within ycf35 at 1820.440 kb on - strand, within ycf35 at 1820.493 kb on + strand, within ycf35 at 1820.518 kb on + strand at 1820.526 kb on - strand at 1820.530 kb on + strand at 1820.545 kb on - strand at 1820.567 kb on - strand at 1820.605 kb on + strand, within Synpcc7942_1751 at 1820.641 kb on - strand, within Synpcc7942_1751 at 1820.656 kb on - strand, within Synpcc7942_1751 at 1820.656 kb on - strand, within Synpcc7942_1751 at 1820.662 kb on + strand, within Synpcc7942_1751 at 1820.686 kb on + strand, within Synpcc7942_1751 at 1820.693 kb on + strand, within Synpcc7942_1751 at 1820.693 kb on + strand, within Synpcc7942_1751 at 1820.696 kb on - strand, within Synpcc7942_1751 at 1820.705 kb on + strand, within Synpcc7942_1751 at 1820.886 kb on + strand at 1820.886 kb on + strand at 1820.886 kb on + strand at 1820.886 kb on + strand at 1820.886 kb on + strand at 1820.886 kb on + strand at 1820.894 kb on - strand at 1820.894 kb on - strand at 1820.894 kb on - strand at 1820.894 kb on - strand at 1820.894 kb on - strand at 1820.894 kb on - strand at 1820.898 kb on + strand at 1820.959 kb on + strand at 1820.968 kb on + strand at 1820.974 kb on + strand at 1820.977 kb on + strand at 1820.985 kb on - strand at 1821.017 kb on - strand at 1821.017 kb on - strand at 1821.022 kb on - strand at 1821.022 kb on - strand at 1821.024 kb on + strand at 1821.046 kb on + strand at 1821.290 kb on - strand, within Synpcc7942_1752 at 1821.342 kb on - strand, within Synpcc7942_1752 at 1821.400 kb on + strand, within Synpcc7942_1752 at 1821.795 kb on + strand at 1821.801 kb on + strand at 1821.810 kb on + strand at 1821.813 kb on - strand at 1821.813 kb on - strand at 1821.837 kb on + strand at 1821.875 kb on + strand at 1821.897 kb on + strand at 1821.905 kb on - strand at 1821.914 kb on - strand at 1821.924 kb on + strand at 1821.940 kb on + strand, within Synpcc7942_1753 at 1821.948 kb on - strand, within Synpcc7942_1753 at 1821.948 kb on - strand, within Synpcc7942_1753 at 1821.976 kb on + strand, within Synpcc7942_1753 at 1821.977 kb on - strand at 1821.984 kb on - strand, within Synpcc7942_1753 at 1821.984 kb on - strand, within Synpcc7942_1753 at 1821.999 kb on + strand, within Synpcc7942_1753 at 1821.999 kb on + strand, within Synpcc7942_1753 at 1822.006 kb on - strand, within Synpcc7942_1753 at 1822.007 kb on - strand, within Synpcc7942_1753 at 1822.016 kb on + strand, within Synpcc7942_1753 at 1822.016 kb on + strand, within Synpcc7942_1753 at 1822.024 kb on - strand, within Synpcc7942_1753 at 1822.048 kb on + strand, within Synpcc7942_1753 at 1822.071 kb on - strand, within Synpcc7942_1753 at 1822.074 kb on + strand, within Synpcc7942_1753 at 1822.082 kb on - strand, within Synpcc7942_1753 at 1822.082 kb on - strand, within Synpcc7942_1753 at 1822.120 kb on - strand, within Synpcc7942_1753 at 1822.134 kb on + strand, within Synpcc7942_1753 at 1822.134 kb on + strand, within Synpcc7942_1753 at 1822.134 kb on + strand, within Synpcc7942_1753 at 1822.134 kb on + strand, within Synpcc7942_1753 at 1822.141 kb on + strand, within Synpcc7942_1753 at 1822.142 kb on - strand, within Synpcc7942_1753 at 1822.182 kb on - strand, within Synpcc7942_1753 at 1822.221 kb on + strand, within Synpcc7942_1753 at 1822.221 kb on + strand, within Synpcc7942_1753 at 1822.226 kb on + strand, within Synpcc7942_1753 at 1822.229 kb on - strand, within Synpcc7942_1753 at 1822.232 kb on + strand, within Synpcc7942_1753 at 1822.240 kb on - strand, within Synpcc7942_1753 at 1822.258 kb on - strand, within Synpcc7942_1753 at 1822.267 kb on - strand, within Synpcc7942_1753 at 1822.267 kb on - strand, within Synpcc7942_1753 at 1822.267 kb on - strand, within Synpcc7942_1753 at 1822.299 kb on - strand, within Synpcc7942_1753 at 1822.321 kb on - strand at 1822.325 kb on + strand at 1822.325 kb on + strand at 1822.325 kb on + strand at 1822.325 kb on + strand at 1822.325 kb on + strand at 1822.333 kb on - strand at 1822.337 kb on + strand at 1822.465 kb on + strand, within Synpcc7942_1754 at 1822.465 kb on + strand, within Synpcc7942_1754 at 1822.465 kb on + strand, within Synpcc7942_1754 at 1822.473 kb on - strand, within Synpcc7942_1754 at 1822.473 kb on - strand, within Synpcc7942_1754 at 1822.482 kb on - strand at 1822.493 kb on - strand at 1822.583 kb on - strand at 1822.600 kb on + strand at 1822.619 kb on + strand at 1822.625 kb on - strand at 1822.625 kb on - strand at 1822.627 kb on + strand at 1822.632 kb on - strand at 1822.681 kb on - strand, within Synpcc7942_1755 at 1822.683 kb on + strand, within Synpcc7942_1755 at 1822.733 kb on + strand, within Synpcc7942_1755 at 1822.741 kb on - strand, within Synpcc7942_1755 at 1822.743 kb on + strand, within Synpcc7942_1755 at 1822.759 kb on + strand, within Synpcc7942_1755
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,820,020 + fer Synpcc7942_1749 0.68 -2.9 1,820,033 - fer Synpcc7942_1749 0.71 -0.7 1,820,047 + fer Synpcc7942_1749 0.75 +0.9 1,820,047 + fer Synpcc7942_1749 0.75 -0.5 1,820,055 - fer Synpcc7942_1749 0.77 -1.6 1,820,059 + fer Synpcc7942_1749 0.78 -1.9 1,820,059 + fer Synpcc7942_1749 0.78 -1.1 1,820,067 - fer Synpcc7942_1749 0.80 +0.6 1,820,142 + +0.8 1,820,143 + +0.1 1,820,143 + +2.3 1,820,149 - -1.1 1,820,201 - ycf35 Synpcc7942_1750 0.14 -0.5 1,820,218 + ycf35 Synpcc7942_1750 0.19 -0.4 1,820,218 - ycf35 Synpcc7942_1750 0.19 -1.3 1,820,227 - ycf35 Synpcc7942_1750 0.21 -1.5 1,820,236 + ycf35 Synpcc7942_1750 0.23 -1.6 1,820,244 - ycf35 Synpcc7942_1750 0.25 +0.0 1,820,249 - ycf35 Synpcc7942_1750 0.27 -1.9 1,820,263 - +2.4 1,820,265 + ycf35 Synpcc7942_1750 0.31 -2.3 1,820,301 - ycf35 Synpcc7942_1750 0.40 -0.6 1,820,302 - ycf35 Synpcc7942_1750 0.40 +1.3 1,820,323 + ycf35 Synpcc7942_1750 0.46 -1.7 1,820,330 + ycf35 Synpcc7942_1750 0.48 -0.3 1,820,331 + ycf35 Synpcc7942_1750 0.48 -1.4 1,820,338 - ycf35 Synpcc7942_1750 0.50 -0.3 1,820,339 - ycf35 Synpcc7942_1750 0.50 -0.2 1,820,339 - ycf35 Synpcc7942_1750 0.50 -1.8 1,820,346 - ycf35 Synpcc7942_1750 0.52 +0.5 1,820,348 + ycf35 Synpcc7942_1750 0.52 -2.3 1,820,372 + ycf35 Synpcc7942_1750 0.58 +0.2 1,820,422 - ycf35 Synpcc7942_1750 0.71 -0.5 1,820,425 - ycf35 Synpcc7942_1750 0.72 -0.5 1,820,432 + ycf35 Synpcc7942_1750 0.74 -1.0 1,820,432 + +0.1 1,820,432 + ycf35 Synpcc7942_1750 0.74 +0.8 1,820,440 - ycf35 Synpcc7942_1750 0.76 -1.0 1,820,493 + ycf35 Synpcc7942_1750 0.90 +0.2 1,820,518 + +0.1 1,820,526 - +0.9 1,820,530 + +0.5 1,820,545 - +1.5 1,820,567 - -2.1 1,820,605 + Synpcc7942_1751 0.15 +0.3 1,820,641 - Synpcc7942_1751 0.32 -0.9 1,820,656 - Synpcc7942_1751 0.40 -3.7 1,820,656 - Synpcc7942_1751 0.40 -0.1 1,820,662 + Synpcc7942_1751 0.43 -2.7 1,820,686 + Synpcc7942_1751 0.54 -0.2 1,820,693 + Synpcc7942_1751 0.58 -1.2 1,820,693 + Synpcc7942_1751 0.58 -2.1 1,820,696 - Synpcc7942_1751 0.59 -1.9 1,820,705 + Synpcc7942_1751 0.64 -3.9 1,820,886 + +1.1 1,820,886 + -1.4 1,820,886 + -2.6 1,820,886 + +0.1 1,820,886 + -2.8 1,820,886 + -0.6 1,820,894 - -1.4 1,820,894 - -0.5 1,820,894 - -0.5 1,820,894 - -3.3 1,820,894 - +1.1 1,820,894 - +1.0 1,820,898 + -1.9 1,820,959 + +0.8 1,820,968 + +0.8 1,820,974 + -3.4 1,820,977 + +1.7 1,820,985 - -1.1 1,821,017 - +0.1 1,821,017 - +1.6 1,821,022 - -0.2 1,821,022 - -1.1 1,821,024 + -0.9 1,821,046 + -2.7 1,821,290 - Synpcc7942_1752 0.36 -1.6 1,821,342 - Synpcc7942_1752 0.43 -2.7 1,821,400 + Synpcc7942_1752 0.51 -1.1 1,821,795 + -3.2 1,821,801 + +2.0 1,821,810 + -1.4 1,821,813 - +0.0 1,821,813 - -2.0 1,821,837 + +0.5 1,821,875 + +4.7 1,821,897 + -0.7 1,821,905 - -1.6 1,821,914 - +3.4 1,821,924 + +1.8 1,821,940 + Synpcc7942_1753 0.11 +1.8 1,821,948 - Synpcc7942_1753 0.12 +2.6 1,821,948 - Synpcc7942_1753 0.12 -1.6 1,821,976 + Synpcc7942_1753 0.18 -0.3 1,821,977 - +0.1 1,821,984 - Synpcc7942_1753 0.20 -2.9 1,821,984 - Synpcc7942_1753 0.20 -2.0 1,821,999 + Synpcc7942_1753 0.23 -1.5 1,821,999 + Synpcc7942_1753 0.23 +0.1 1,822,006 - Synpcc7942_1753 0.25 +0.8 1,822,007 - Synpcc7942_1753 0.25 +1.6 1,822,016 + Synpcc7942_1753 0.27 +1.3 1,822,016 + Synpcc7942_1753 0.27 +0.1 1,822,024 - Synpcc7942_1753 0.29 +2.7 1,822,048 + Synpcc7942_1753 0.34 -2.0 1,822,071 - Synpcc7942_1753 0.39 +1.7 1,822,074 + Synpcc7942_1753 0.40 -0.1 1,822,082 - Synpcc7942_1753 0.41 -0.6 1,822,082 - Synpcc7942_1753 0.41 -1.7 1,822,120 - Synpcc7942_1753 0.49 -1.1 1,822,134 + Synpcc7942_1753 0.52 -0.9 1,822,134 + Synpcc7942_1753 0.52 +5.9 1,822,134 + Synpcc7942_1753 0.52 +1.1 1,822,134 + Synpcc7942_1753 0.52 +1.1 1,822,141 + Synpcc7942_1753 0.54 +0.5 1,822,142 - Synpcc7942_1753 0.54 -0.3 1,822,182 - Synpcc7942_1753 0.63 +0.7 1,822,221 + Synpcc7942_1753 0.71 -1.6 1,822,221 + Synpcc7942_1753 0.71 -1.4 1,822,226 + Synpcc7942_1753 0.72 +0.3 1,822,229 - Synpcc7942_1753 0.73 +1.2 1,822,232 + Synpcc7942_1753 0.74 +0.3 1,822,240 - Synpcc7942_1753 0.75 -1.4 1,822,258 - Synpcc7942_1753 0.79 -3.0 1,822,267 - Synpcc7942_1753 0.81 +0.8 1,822,267 - Synpcc7942_1753 0.81 +0.7 1,822,267 - Synpcc7942_1753 0.81 +0.4 1,822,299 - Synpcc7942_1753 0.88 -2.5 1,822,321 - +2.2 1,822,325 + +1.5 1,822,325 + +1.1 1,822,325 + -1.1 1,822,325 + -0.2 1,822,325 + -1.9 1,822,333 - -0.6 1,822,337 + +2.4 1,822,465 + Synpcc7942_1754 0.78 +1.0 1,822,465 + Synpcc7942_1754 0.78 -3.6 1,822,465 + Synpcc7942_1754 0.78 +2.2 1,822,473 - Synpcc7942_1754 0.84 +0.9 1,822,473 - Synpcc7942_1754 0.84 +0.5 1,822,482 - +0.9 1,822,493 - +2.0 1,822,583 - -1.9 1,822,600 + -0.9 1,822,619 + +1.5 1,822,625 - -1.6 1,822,625 - -0.7 1,822,627 + +2.4 1,822,632 - +0.9 1,822,681 - Synpcc7942_1755 0.11 +2.8 1,822,683 + Synpcc7942_1755 0.11 +0.6 1,822,733 + Synpcc7942_1755 0.14 +2.0 1,822,741 - Synpcc7942_1755 0.14 -1.6 1,822,743 + Synpcc7942_1755 0.14 +2.4 1,822,759 + Synpcc7942_1755 0.15 +0.5
Or see this region's nucleotide sequence