Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1744 and Synpcc7942_1745 are separated by 14 nucleotides Synpcc7942_1745 and rpoD2 are separated by 206 nucleotides rpoD2 and Synpcc7942_1747 are separated by 215 nucleotides
Synpcc7942_1744: Synpcc7942_1744 - hypothetical protein, at 1,815,423 to 1,815,833
_1744
Synpcc7942_1745: Synpcc7942_1745 - nitrite transporter, HPP family (from data) , at 1,815,848 to 1,816,393
_1745
Synpcc7942_1746: rpoD2 - group2 RNA polymerase sigma factor SigB, at 1,816,600 to 1,817,562
rpoD2
Synpcc7942_1747: Synpcc7942_1747 - hypothetical protein, at 1,817,778 to 1,818,962
_1747
Position (kb)
1816
1817
1818 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 1815.847 kb on + strand at 1815.857 kb on + strand at 1815.864 kb on + strand at 1815.864 kb on + strand at 1815.865 kb on - strand at 1815.865 kb on - strand at 1815.867 kb on - strand at 1815.867 kb on - strand at 1815.872 kb on - strand at 1815.900 kb on + strand at 1815.915 kb on + strand, within Synpcc7942_1745 at 1815.928 kb on + strand, within Synpcc7942_1745 at 1815.931 kb on - strand, within Synpcc7942_1745 at 1815.936 kb on - strand, within Synpcc7942_1745 at 1815.946 kb on - strand, within Synpcc7942_1745 at 1815.946 kb on - strand, within Synpcc7942_1745 at 1815.957 kb on + strand, within Synpcc7942_1745 at 1816.005 kb on - strand, within Synpcc7942_1745 at 1816.015 kb on + strand, within Synpcc7942_1745 at 1816.018 kb on + strand, within Synpcc7942_1745 at 1816.066 kb on + strand, within Synpcc7942_1745 at 1816.074 kb on + strand, within Synpcc7942_1745 at 1816.089 kb on + strand, within Synpcc7942_1745 at 1816.089 kb on - strand, within Synpcc7942_1745 at 1816.089 kb on - strand, within Synpcc7942_1745 at 1816.123 kb on - strand, within Synpcc7942_1745 at 1816.160 kb on + strand, within Synpcc7942_1745 at 1816.193 kb on + strand, within Synpcc7942_1745 at 1816.195 kb on - strand, within Synpcc7942_1745 at 1816.216 kb on - strand, within Synpcc7942_1745 at 1816.311 kb on + strand, within Synpcc7942_1745 at 1816.321 kb on + strand, within Synpcc7942_1745 at 1816.354 kb on - strand at 1816.436 kb on - strand at 1816.526 kb on + strand at 1816.534 kb on - strand at 1816.573 kb on + strand at 1816.599 kb on - strand at 1816.656 kb on - strand at 1816.735 kb on - strand, within rpoD2 at 1816.745 kb on + strand, within rpoD2 at 1816.745 kb on - strand, within rpoD2 at 1816.841 kb on - strand, within rpoD2 at 1816.925 kb on - strand, within rpoD2 at 1816.969 kb on + strand, within rpoD2 at 1817.113 kb on + strand, within rpoD2 at 1817.113 kb on + strand, within rpoD2 at 1817.113 kb on + strand, within rpoD2 at 1817.113 kb on + strand, within rpoD2 at 1817.113 kb on + strand, within rpoD2 at 1817.121 kb on - strand, within rpoD2 at 1817.121 kb on - strand, within rpoD2 at 1817.121 kb on - strand, within rpoD2 at 1817.121 kb on - strand, within rpoD2 at 1817.148 kb on - strand, within rpoD2 at 1817.163 kb on - strand, within rpoD2 at 1817.170 kb on - strand, within rpoD2 at 1817.252 kb on - strand, within rpoD2 at 1817.322 kb on + strand, within rpoD2 at 1817.373 kb on + strand, within rpoD2 at 1817.388 kb on + strand, within rpoD2 at 1817.395 kb on - strand, within rpoD2 at 1817.396 kb on - strand, within rpoD2 at 1817.396 kb on - strand, within rpoD2 at 1817.472 kb on - strand at 1817.478 kb on - strand at 1817.514 kb on + strand at 1817.519 kb on + strand at 1817.522 kb on - strand at 1817.524 kb on + strand at 1817.524 kb on + strand at 1817.532 kb on - strand at 1817.539 kb on + strand at 1817.539 kb on + strand at 1817.539 kb on + strand at 1817.539 kb on + strand at 1817.539 kb on + strand at 1817.547 kb on - strand at 1817.547 kb on - strand at 1817.547 kb on - strand at 1817.547 kb on - strand at 1817.547 kb on - strand at 1817.620 kb on - strand at 1817.680 kb on + strand at 1817.704 kb on + strand at 1817.712 kb on - strand at 1817.727 kb on + strand at 1817.809 kb on - strand at 1817.867 kb on + strand at 1817.874 kb on + strand at 1817.899 kb on + strand, within Synpcc7942_1747 at 1817.909 kb on + strand, within Synpcc7942_1747 at 1817.909 kb on + strand, within Synpcc7942_1747 at 1817.914 kb on + strand, within Synpcc7942_1747 at 1817.915 kb on - strand, within Synpcc7942_1747 at 1817.917 kb on - strand, within Synpcc7942_1747 at 1817.917 kb on - strand, within Synpcc7942_1747 at 1818.056 kb on - strand, within Synpcc7942_1747 at 1818.056 kb on - strand, within Synpcc7942_1747 at 1818.058 kb on - strand, within Synpcc7942_1747 at 1818.058 kb on - strand, within Synpcc7942_1747 at 1818.058 kb on - strand, within Synpcc7942_1747 at 1818.058 kb on - strand, within Synpcc7942_1747 at 1818.071 kb on + strand, within Synpcc7942_1747 at 1818.073 kb on + strand, within Synpcc7942_1747 at 1818.073 kb on + strand, within Synpcc7942_1747 at 1818.090 kb on + strand, within Synpcc7942_1747 at 1818.096 kb on + strand, within Synpcc7942_1747 at 1818.098 kb on - strand, within Synpcc7942_1747 at 1818.101 kb on + strand, within Synpcc7942_1747 at 1818.101 kb on + strand, within Synpcc7942_1747 at 1818.118 kb on + strand, within Synpcc7942_1747 at 1818.129 kb on + strand, within Synpcc7942_1747 at 1818.155 kb on - strand, within Synpcc7942_1747 at 1818.186 kb on - strand, within Synpcc7942_1747 at 1818.212 kb on - strand, within Synpcc7942_1747 at 1818.220 kb on + strand, within Synpcc7942_1747 at 1818.227 kb on + strand, within Synpcc7942_1747 at 1818.228 kb on + strand, within Synpcc7942_1747 at 1818.234 kb on + strand, within Synpcc7942_1747 at 1818.250 kb on + strand, within Synpcc7942_1747 at 1818.279 kb on - strand, within Synpcc7942_1747 at 1818.287 kb on - strand, within Synpcc7942_1747 at 1818.319 kb on + strand, within Synpcc7942_1747 at 1818.319 kb on + strand, within Synpcc7942_1747 at 1818.319 kb on + strand, within Synpcc7942_1747 at 1818.319 kb on + strand, within Synpcc7942_1747 at 1818.319 kb on + strand, within Synpcc7942_1747 at 1818.319 kb on + strand, within Synpcc7942_1747 at 1818.319 kb on + strand, within Synpcc7942_1747 at 1818.327 kb on - strand, within Synpcc7942_1747 at 1818.327 kb on - strand, within Synpcc7942_1747 at 1818.327 kb on - strand, within Synpcc7942_1747 at 1818.327 kb on - strand, within Synpcc7942_1747 at 1818.328 kb on + strand, within Synpcc7942_1747 at 1818.338 kb on + strand, within Synpcc7942_1747 at 1818.360 kb on - strand, within Synpcc7942_1747 at 1818.368 kb on + strand, within Synpcc7942_1747 at 1818.370 kb on - strand, within Synpcc7942_1747 at 1818.410 kb on + strand, within Synpcc7942_1747 at 1818.423 kb on - strand, within Synpcc7942_1747 at 1818.423 kb on - strand, within Synpcc7942_1747 at 1818.435 kb on + strand, within Synpcc7942_1747 at 1818.516 kb on + strand, within Synpcc7942_1747 at 1818.526 kb on + strand, within Synpcc7942_1747 at 1818.545 kb on + strand, within Synpcc7942_1747 at 1818.559 kb on + strand, within Synpcc7942_1747
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,815,847 + +3.3 1,815,857 + +2.4 1,815,864 + +0.8 1,815,864 + +1.5 1,815,865 - -1.0 1,815,865 - -1.7 1,815,867 - +0.2 1,815,867 - +2.2 1,815,872 - +0.8 1,815,900 + +1.0 1,815,915 + Synpcc7942_1745 0.12 +4.2 1,815,928 + Synpcc7942_1745 0.15 +1.8 1,815,931 - Synpcc7942_1745 0.15 -0.4 1,815,936 - Synpcc7942_1745 0.16 +1.5 1,815,946 - Synpcc7942_1745 0.18 +1.3 1,815,946 - Synpcc7942_1745 0.18 +0.5 1,815,957 + Synpcc7942_1745 0.20 +1.8 1,816,005 - Synpcc7942_1745 0.29 +0.3 1,816,015 + Synpcc7942_1745 0.31 +0.5 1,816,018 + Synpcc7942_1745 0.31 +2.0 1,816,066 + Synpcc7942_1745 0.40 -2.9 1,816,074 + Synpcc7942_1745 0.41 -0.1 1,816,089 + Synpcc7942_1745 0.44 -0.4 1,816,089 - Synpcc7942_1745 0.44 +1.9 1,816,089 - Synpcc7942_1745 0.44 +0.3 1,816,123 - Synpcc7942_1745 0.50 +1.2 1,816,160 + Synpcc7942_1745 0.57 -0.6 1,816,193 + Synpcc7942_1745 0.63 +2.9 1,816,195 - Synpcc7942_1745 0.64 -0.0 1,816,216 - Synpcc7942_1745 0.67 +0.8 1,816,311 + Synpcc7942_1745 0.85 +1.3 1,816,321 + Synpcc7942_1745 0.87 +1.0 1,816,354 - +0.9 1,816,436 - -0.9 1,816,526 + -0.2 1,816,534 - -1.8 1,816,573 + +2.2 1,816,599 - -0.1 1,816,656 - -5.5 1,816,735 - rpoD2 Synpcc7942_1746 0.14 -3.5 1,816,745 + rpoD2 Synpcc7942_1746 0.15 -5.0 1,816,745 - rpoD2 Synpcc7942_1746 0.15 -1.2 1,816,841 - rpoD2 Synpcc7942_1746 0.25 -5.1 1,816,925 - rpoD2 Synpcc7942_1746 0.34 -3.4 1,816,969 + rpoD2 Synpcc7942_1746 0.38 -2.0 1,817,113 + rpoD2 Synpcc7942_1746 0.53 -1.8 1,817,113 + rpoD2 Synpcc7942_1746 0.53 -2.0 1,817,113 + rpoD2 Synpcc7942_1746 0.53 -2.2 1,817,113 + rpoD2 Synpcc7942_1746 0.53 -2.7 1,817,113 + rpoD2 Synpcc7942_1746 0.53 -3.2 1,817,121 - rpoD2 Synpcc7942_1746 0.54 -3.8 1,817,121 - rpoD2 Synpcc7942_1746 0.54 -3.5 1,817,121 - rpoD2 Synpcc7942_1746 0.54 -3.7 1,817,121 - rpoD2 Synpcc7942_1746 0.54 -2.8 1,817,148 - rpoD2 Synpcc7942_1746 0.57 -1.0 1,817,163 - rpoD2 Synpcc7942_1746 0.58 -4.9 1,817,170 - rpoD2 Synpcc7942_1746 0.59 -2.7 1,817,252 - rpoD2 Synpcc7942_1746 0.68 -3.3 1,817,322 + rpoD2 Synpcc7942_1746 0.75 -1.5 1,817,373 + rpoD2 Synpcc7942_1746 0.80 -4.2 1,817,388 + rpoD2 Synpcc7942_1746 0.82 -1.9 1,817,395 - rpoD2 Synpcc7942_1746 0.83 -2.2 1,817,396 - rpoD2 Synpcc7942_1746 0.83 -2.8 1,817,396 - rpoD2 Synpcc7942_1746 0.83 -3.5 1,817,472 - -5.8 1,817,478 - -4.3 1,817,514 + -5.7 1,817,519 + -2.1 1,817,522 - -3.1 1,817,524 + -1.5 1,817,524 + -3.2 1,817,532 - -3.8 1,817,539 + -2.2 1,817,539 + -4.7 1,817,539 + -0.5 1,817,539 + -4.2 1,817,539 + -1.2 1,817,547 - -4.6 1,817,547 - -2.5 1,817,547 - -3.9 1,817,547 - -2.2 1,817,547 - -2.4 1,817,620 - -3.3 1,817,680 + -2.2 1,817,704 + -2.6 1,817,712 - -4.6 1,817,727 + -3.1 1,817,809 - -2.1 1,817,867 + +0.2 1,817,874 + +2.5 1,817,899 + Synpcc7942_1747 0.10 -1.2 1,817,909 + Synpcc7942_1747 0.11 -0.8 1,817,909 + Synpcc7942_1747 0.11 -0.1 1,817,914 + Synpcc7942_1747 0.11 -1.6 1,817,915 - Synpcc7942_1747 0.12 -1.1 1,817,917 - Synpcc7942_1747 0.12 +1.1 1,817,917 - Synpcc7942_1747 0.12 +0.4 1,818,056 - Synpcc7942_1747 0.23 +0.3 1,818,056 - Synpcc7942_1747 0.23 -2.2 1,818,058 - Synpcc7942_1747 0.24 -2.0 1,818,058 - Synpcc7942_1747 0.24 -2.5 1,818,058 - Synpcc7942_1747 0.24 -1.7 1,818,058 - Synpcc7942_1747 0.24 -2.7 1,818,071 + Synpcc7942_1747 0.25 -0.1 1,818,073 + Synpcc7942_1747 0.25 +1.1 1,818,073 + Synpcc7942_1747 0.25 +1.1 1,818,090 + Synpcc7942_1747 0.26 -1.0 1,818,096 + Synpcc7942_1747 0.27 +1.4 1,818,098 - Synpcc7942_1747 0.27 +1.2 1,818,101 + Synpcc7942_1747 0.27 -3.2 1,818,101 + Synpcc7942_1747 0.27 +0.8 1,818,118 + Synpcc7942_1747 0.29 +0.4 1,818,129 + Synpcc7942_1747 0.30 -1.6 1,818,155 - Synpcc7942_1747 0.32 -0.4 1,818,186 - Synpcc7942_1747 0.34 -1.6 1,818,212 - Synpcc7942_1747 0.37 -1.5 1,818,220 + Synpcc7942_1747 0.37 -2.3 1,818,227 + Synpcc7942_1747 0.38 -0.2 1,818,228 + Synpcc7942_1747 0.38 +1.5 1,818,234 + Synpcc7942_1747 0.38 +3.3 1,818,250 + Synpcc7942_1747 0.40 -1.2 1,818,279 - Synpcc7942_1747 0.42 -1.0 1,818,287 - Synpcc7942_1747 0.43 +1.9 1,818,319 + Synpcc7942_1747 0.46 +0.8 1,818,319 + Synpcc7942_1747 0.46 +2.6 1,818,319 + Synpcc7942_1747 0.46 -0.5 1,818,319 + Synpcc7942_1747 0.46 -2.1 1,818,319 + Synpcc7942_1747 0.46 +1.1 1,818,319 + Synpcc7942_1747 0.46 -3.3 1,818,319 + Synpcc7942_1747 0.46 +2.2 1,818,327 - Synpcc7942_1747 0.46 -1.1 1,818,327 - Synpcc7942_1747 0.46 +0.6 1,818,327 - Synpcc7942_1747 0.46 +1.5 1,818,327 - Synpcc7942_1747 0.46 -1.9 1,818,328 + Synpcc7942_1747 0.46 -0.3 1,818,338 + Synpcc7942_1747 0.47 -0.2 1,818,360 - Synpcc7942_1747 0.49 +1.1 1,818,368 + Synpcc7942_1747 0.50 -3.0 1,818,370 - Synpcc7942_1747 0.50 +0.4 1,818,410 + Synpcc7942_1747 0.53 -2.6 1,818,423 - Synpcc7942_1747 0.54 +1.5 1,818,423 - Synpcc7942_1747 0.54 -2.3 1,818,435 + Synpcc7942_1747 0.55 +2.6 1,818,516 + Synpcc7942_1747 0.62 +1.8 1,818,526 + Synpcc7942_1747 0.63 -0.2 1,818,545 + Synpcc7942_1747 0.65 -1.2 1,818,559 + Synpcc7942_1747 0.66 +0.9
Or see this region's nucleotide sequence