Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt katG and Synpcc7942_1657 are separated by 371 nucleotides Synpcc7942_1657 and Synpcc7942_1658 are separated by 113 nucleotides Synpcc7942_1658 and Synpcc7942_1659 are separated by 137 nucleotides Synpcc7942_1659 and Synpcc7942_1660 are separated by 99 nucleotides
Synpcc7942_1656: katG - catalase/peroxidase HPI, at 1,725,919 to 1,728,081
katG
Synpcc7942_1657: Synpcc7942_1657 - hypothetical protein, at 1,728,453 to 1,728,674
_1657
Synpcc7942_1658: Synpcc7942_1658 - hypothetical protein, at 1,728,788 to 1,729,246
_1658
Synpcc7942_1659: Synpcc7942_1659 - nitroreductase, at 1,729,384 to 1,730,031
_1659
Synpcc7942_1660: Synpcc7942_1660 - potassium channel protein, at 1,730,131 to 1,730,964
_1660
Position (kb)
1728
1729
1730 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1727.823 kb on - strand, within katG at 1727.895 kb on + strand at 1728.046 kb on + strand at 1728.206 kb on - strand at 1728.243 kb on - strand at 1728.245 kb on + strand at 1728.250 kb on - strand at 1728.281 kb on - strand at 1728.378 kb on + strand at 1728.401 kb on - strand at 1728.405 kb on + strand at 1728.408 kb on - strand at 1728.410 kb on + strand at 1728.421 kb on + strand at 1728.422 kb on + strand at 1728.422 kb on + strand at 1728.422 kb on + strand at 1728.512 kb on + strand, within Synpcc7942_1657 at 1728.515 kb on + strand, within Synpcc7942_1657 at 1728.535 kb on - strand, within Synpcc7942_1657 at 1728.548 kb on + strand, within Synpcc7942_1657 at 1728.569 kb on - strand, within Synpcc7942_1657 at 1728.569 kb on - strand, within Synpcc7942_1657 at 1728.643 kb on + strand, within Synpcc7942_1657 at 1728.643 kb on + strand, within Synpcc7942_1657 at 1728.648 kb on + strand, within Synpcc7942_1657 at 1728.661 kb on - strand at 1728.715 kb on + strand at 1728.733 kb on - strand at 1728.735 kb on + strand at 1728.735 kb on + strand at 1728.743 kb on - strand at 1728.743 kb on - strand at 1728.751 kb on - strand at 1728.785 kb on - strand at 1728.875 kb on + strand, within Synpcc7942_1658 at 1728.875 kb on + strand, within Synpcc7942_1658 at 1728.883 kb on - strand, within Synpcc7942_1658 at 1728.951 kb on - strand, within Synpcc7942_1658 at 1728.951 kb on - strand at 1728.955 kb on + strand, within Synpcc7942_1658 at 1728.965 kb on + strand, within Synpcc7942_1658 at 1728.982 kb on + strand, within Synpcc7942_1658 at 1728.999 kb on + strand, within Synpcc7942_1658 at 1729.002 kb on - strand, within Synpcc7942_1658 at 1729.007 kb on - strand, within Synpcc7942_1658 at 1729.018 kb on - strand, within Synpcc7942_1658 at 1729.030 kb on - strand, within Synpcc7942_1658 at 1729.034 kb on + strand, within Synpcc7942_1658 at 1729.034 kb on + strand, within Synpcc7942_1658 at 1729.045 kb on + strand, within Synpcc7942_1658 at 1729.045 kb on + strand, within Synpcc7942_1658 at 1729.045 kb on + strand, within Synpcc7942_1658 at 1729.045 kb on + strand, within Synpcc7942_1658 at 1729.100 kb on + strand, within Synpcc7942_1658 at 1729.108 kb on - strand, within Synpcc7942_1658 at 1729.163 kb on - strand, within Synpcc7942_1658 at 1729.186 kb on + strand, within Synpcc7942_1658 at 1729.188 kb on - strand, within Synpcc7942_1658 at 1729.194 kb on - strand, within Synpcc7942_1658 at 1729.194 kb on - strand, within Synpcc7942_1658 at 1729.194 kb on - strand, within Synpcc7942_1658 at 1729.217 kb on - strand at 1729.223 kb on + strand at 1729.223 kb on + strand at 1729.223 kb on + strand at 1729.231 kb on - strand at 1729.244 kb on - strand at 1729.255 kb on - strand at 1729.272 kb on - strand at 1729.293 kb on - strand at 1729.311 kb on + strand at 1729.311 kb on + strand at 1729.319 kb on - strand at 1729.340 kb on + strand at 1729.359 kb on - strand at 1729.359 kb on - strand at 1729.361 kb on + strand at 1729.374 kb on - strand at 1729.387 kb on - strand at 1729.415 kb on - strand at 1729.423 kb on - strand at 1729.483 kb on - strand, within Synpcc7942_1659 at 1729.496 kb on + strand, within Synpcc7942_1659 at 1729.496 kb on + strand, within Synpcc7942_1659 at 1729.496 kb on + strand, within Synpcc7942_1659 at 1729.501 kb on + strand, within Synpcc7942_1659 at 1729.504 kb on - strand, within Synpcc7942_1659 at 1729.504 kb on - strand, within Synpcc7942_1659 at 1729.514 kb on + strand, within Synpcc7942_1659 at 1729.520 kb on - strand, within Synpcc7942_1659 at 1729.531 kb on - strand, within Synpcc7942_1659 at 1729.532 kb on - strand, within Synpcc7942_1659 at 1729.556 kb on + strand, within Synpcc7942_1659 at 1729.564 kb on - strand, within Synpcc7942_1659 at 1729.564 kb on - strand, within Synpcc7942_1659 at 1729.564 kb on - strand, within Synpcc7942_1659 at 1729.564 kb on - strand, within Synpcc7942_1659 at 1729.589 kb on + strand, within Synpcc7942_1659 at 1729.589 kb on + strand, within Synpcc7942_1659 at 1729.589 kb on + strand, within Synpcc7942_1659 at 1729.589 kb on + strand, within Synpcc7942_1659 at 1729.597 kb on - strand, within Synpcc7942_1659 at 1729.597 kb on - strand, within Synpcc7942_1659 at 1729.597 kb on - strand, within Synpcc7942_1659 at 1729.597 kb on - strand, within Synpcc7942_1659 at 1729.599 kb on + strand, within Synpcc7942_1659 at 1729.606 kb on - strand, within Synpcc7942_1659 at 1729.618 kb on - strand, within Synpcc7942_1659 at 1729.651 kb on - strand, within Synpcc7942_1659 at 1729.659 kb on + strand, within Synpcc7942_1659 at 1729.663 kb on - strand, within Synpcc7942_1659 at 1729.678 kb on + strand, within Synpcc7942_1659 at 1729.769 kb on + strand, within Synpcc7942_1659 at 1729.785 kb on + strand, within Synpcc7942_1659 at 1729.785 kb on + strand, within Synpcc7942_1659 at 1729.798 kb on + strand, within Synpcc7942_1659 at 1729.803 kb on + strand, within Synpcc7942_1659 at 1729.803 kb on + strand, within Synpcc7942_1659 at 1729.840 kb on + strand, within Synpcc7942_1659 at 1729.855 kb on + strand, within Synpcc7942_1659 at 1729.908 kb on + strand, within Synpcc7942_1659 at 1729.909 kb on + strand, within Synpcc7942_1659 at 1729.909 kb on + strand, within Synpcc7942_1659 at 1729.915 kb on - strand, within Synpcc7942_1659 at 1729.917 kb on - strand, within Synpcc7942_1659 at 1729.917 kb on - strand, within Synpcc7942_1659 at 1729.933 kb on + strand, within Synpcc7942_1659 at 1729.933 kb on + strand, within Synpcc7942_1659 at 1729.941 kb on - strand, within Synpcc7942_1659 at 1729.941 kb on - strand, within Synpcc7942_1659 at 1729.953 kb on - strand, within Synpcc7942_1659 at 1729.981 kb on + strand at 1729.981 kb on + strand at 1729.981 kb on + strand at 1730.045 kb on + strand at 1730.047 kb on - strand at 1730.047 kb on - strand at 1730.060 kb on + strand at 1730.071 kb on + strand at 1730.079 kb on - strand at 1730.079 kb on - strand at 1730.091 kb on - strand at 1730.123 kb on + strand at 1730.131 kb on - strand at 1730.199 kb on - strand at 1730.199 kb on - strand at 1730.211 kb on - strand at 1730.211 kb on - strand at 1730.212 kb on - strand at 1730.212 kb on - strand at 1730.212 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,727,823 - katG Synpcc7942_1656 0.88 -2.6 1,727,895 + +1.4 1,728,046 + -0.2 1,728,206 - +0.9 1,728,243 - -0.3 1,728,245 + +0.3 1,728,250 - +1.8 1,728,281 - -2.9 1,728,378 + +1.3 1,728,401 - -0.6 1,728,405 + -1.8 1,728,408 - +0.5 1,728,410 + -1.4 1,728,421 + +1.1 1,728,422 + -1.3 1,728,422 + -0.3 1,728,422 + -1.6 1,728,512 + Synpcc7942_1657 0.27 +2.8 1,728,515 + Synpcc7942_1657 0.28 +0.6 1,728,535 - Synpcc7942_1657 0.37 +1.8 1,728,548 + Synpcc7942_1657 0.43 -0.1 1,728,569 - Synpcc7942_1657 0.52 +0.7 1,728,569 - Synpcc7942_1657 0.52 +3.3 1,728,643 + Synpcc7942_1657 0.86 -2.0 1,728,643 + Synpcc7942_1657 0.86 -0.5 1,728,648 + Synpcc7942_1657 0.88 +0.8 1,728,661 - +1.4 1,728,715 + +0.7 1,728,733 - -0.8 1,728,735 + -1.6 1,728,735 + -0.3 1,728,743 - +1.0 1,728,743 - +0.8 1,728,751 - +1.5 1,728,785 - -2.8 1,728,875 + Synpcc7942_1658 0.19 -1.7 1,728,875 + Synpcc7942_1658 0.19 +3.5 1,728,883 - Synpcc7942_1658 0.21 -2.9 1,728,951 - Synpcc7942_1658 0.36 -2.5 1,728,951 - +0.5 1,728,955 + Synpcc7942_1658 0.36 -2.5 1,728,965 + Synpcc7942_1658 0.39 -1.5 1,728,982 + Synpcc7942_1658 0.42 +1.5 1,728,999 + Synpcc7942_1658 0.46 +1.2 1,729,002 - Synpcc7942_1658 0.47 -3.2 1,729,007 - Synpcc7942_1658 0.48 -1.0 1,729,018 - Synpcc7942_1658 0.50 +0.7 1,729,030 - Synpcc7942_1658 0.53 +1.2 1,729,034 + Synpcc7942_1658 0.54 -2.5 1,729,034 + Synpcc7942_1658 0.54 -1.5 1,729,045 + Synpcc7942_1658 0.56 +1.9 1,729,045 + Synpcc7942_1658 0.56 -1.1 1,729,045 + Synpcc7942_1658 0.56 -0.5 1,729,045 + Synpcc7942_1658 0.56 -1.7 1,729,100 + Synpcc7942_1658 0.68 +2.1 1,729,108 - Synpcc7942_1658 0.70 -1.4 1,729,163 - Synpcc7942_1658 0.82 +1.2 1,729,186 + Synpcc7942_1658 0.87 +1.2 1,729,188 - Synpcc7942_1658 0.87 -0.7 1,729,194 - Synpcc7942_1658 0.88 -2.0 1,729,194 - Synpcc7942_1658 0.88 -0.6 1,729,194 - Synpcc7942_1658 0.88 +1.7 1,729,217 - -1.5 1,729,223 + +1.0 1,729,223 + +1.0 1,729,223 + -0.8 1,729,231 - +0.1 1,729,244 - +1.9 1,729,255 - +0.8 1,729,272 - +1.8 1,729,293 - -0.7 1,729,311 + -1.8 1,729,311 + -0.3 1,729,319 - -0.8 1,729,340 + -1.7 1,729,359 - +1.6 1,729,359 - +0.9 1,729,361 + -1.5 1,729,374 - +1.8 1,729,387 - -1.4 1,729,415 - +1.8 1,729,423 - -3.0 1,729,483 - Synpcc7942_1659 0.15 -1.1 1,729,496 + Synpcc7942_1659 0.17 +2.4 1,729,496 + Synpcc7942_1659 0.17 +0.2 1,729,496 + Synpcc7942_1659 0.17 -3.1 1,729,501 + Synpcc7942_1659 0.18 -1.4 1,729,504 - Synpcc7942_1659 0.19 -1.0 1,729,504 - Synpcc7942_1659 0.19 -1.9 1,729,514 + Synpcc7942_1659 0.20 +1.0 1,729,520 - Synpcc7942_1659 0.21 -0.3 1,729,531 - Synpcc7942_1659 0.23 +1.4 1,729,532 - Synpcc7942_1659 0.23 +1.4 1,729,556 + Synpcc7942_1659 0.27 -1.3 1,729,564 - Synpcc7942_1659 0.28 -2.4 1,729,564 - Synpcc7942_1659 0.28 +1.7 1,729,564 - Synpcc7942_1659 0.28 +0.3 1,729,564 - Synpcc7942_1659 0.28 -0.1 1,729,589 + Synpcc7942_1659 0.32 +1.3 1,729,589 + Synpcc7942_1659 0.32 -2.4 1,729,589 + Synpcc7942_1659 0.32 -1.1 1,729,589 + Synpcc7942_1659 0.32 +1.4 1,729,597 - Synpcc7942_1659 0.33 +0.6 1,729,597 - Synpcc7942_1659 0.33 -0.7 1,729,597 - Synpcc7942_1659 0.33 +1.4 1,729,597 - Synpcc7942_1659 0.33 -0.8 1,729,599 + Synpcc7942_1659 0.33 -0.9 1,729,606 - Synpcc7942_1659 0.34 -1.1 1,729,618 - Synpcc7942_1659 0.36 +0.4 1,729,651 - Synpcc7942_1659 0.41 -1.6 1,729,659 + Synpcc7942_1659 0.42 +1.6 1,729,663 - Synpcc7942_1659 0.43 -0.3 1,729,678 + Synpcc7942_1659 0.45 +1.9 1,729,769 + Synpcc7942_1659 0.59 +2.4 1,729,785 + Synpcc7942_1659 0.62 +1.8 1,729,785 + Synpcc7942_1659 0.62 -2.9 1,729,798 + Synpcc7942_1659 0.64 -1.1 1,729,803 + Synpcc7942_1659 0.65 -0.3 1,729,803 + Synpcc7942_1659 0.65 +1.5 1,729,840 + Synpcc7942_1659 0.70 -2.6 1,729,855 + Synpcc7942_1659 0.73 +2.5 1,729,908 + Synpcc7942_1659 0.81 +1.7 1,729,909 + Synpcc7942_1659 0.81 +2.3 1,729,909 + Synpcc7942_1659 0.81 -0.8 1,729,915 - Synpcc7942_1659 0.82 -1.5 1,729,917 - Synpcc7942_1659 0.82 +1.1 1,729,917 - Synpcc7942_1659 0.82 +1.3 1,729,933 + Synpcc7942_1659 0.85 +0.3 1,729,933 + Synpcc7942_1659 0.85 -2.6 1,729,941 - Synpcc7942_1659 0.86 +1.6 1,729,941 - Synpcc7942_1659 0.86 -0.7 1,729,953 - Synpcc7942_1659 0.88 +1.3 1,729,981 + +2.1 1,729,981 + -0.0 1,729,981 + +0.9 1,730,045 + +1.3 1,730,047 - -1.9 1,730,047 - -2.5 1,730,060 + +0.2 1,730,071 + +0.6 1,730,079 - -3.0 1,730,079 - +1.4 1,730,091 - +0.6 1,730,123 + -2.1 1,730,131 - +1.2 1,730,199 - -1.2 1,730,199 - +1.1 1,730,211 - -2.1 1,730,211 - +2.2 1,730,212 - +1.3 1,730,212 - -0.9 1,730,212 - -0.7
Or see this region's nucleotide sequence