Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Gly and Synpcc7942_1565 are separated by 100 nucleotides Synpcc7942_1565 and ppk are separated by 38 nucleotides ppk and Synpcc7942_1567 are separated by 57 nucleotides Synpcc7942_1567 and Synpcc7942_1568 are separated by 337 nucleotides
Synpcc7942_R0001: tRNA-Gly - tRNA-Gly, at 1,622,798 to 1,622,869
tRNA-Gly
Synpcc7942_1565: Synpcc7942_1565 - hypothetical protein, at 1,622,970 to 1,623,410
_1565
Synpcc7942_1566: ppk - polyphosphate kinase, at 1,623,449 to 1,625,644
ppk
Synpcc7942_1567: Synpcc7942_1567 - possible ribosomal protein L36, at 1,625,702 to 1,626,019
_1567
Synpcc7942_1568: Synpcc7942_1568 - hypothetical protein, at 1,626,357 to 1,626,596
_1568
Position (kb)
1623
1624
1625
1626 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1622.468 kb on + strand at 1622.477 kb on - strand at 1622.869 kb on + strand at 1622.877 kb on - strand at 1622.919 kb on + strand at 1622.919 kb on + strand at 1622.921 kb on + strand at 1622.931 kb on + strand at 1622.950 kb on - strand at 1622.960 kb on - strand at 1622.966 kb on - strand at 1622.987 kb on - strand at 1622.991 kb on + strand at 1622.999 kb on - strand at 1623.038 kb on + strand, within Synpcc7942_1565 at 1623.102 kb on + strand, within Synpcc7942_1565 at 1623.114 kb on - strand, within Synpcc7942_1565 at 1623.146 kb on - strand, within Synpcc7942_1565 at 1623.255 kb on + strand, within Synpcc7942_1565 at 1623.265 kb on - strand, within Synpcc7942_1565 at 1623.270 kb on - strand, within Synpcc7942_1565 at 1623.329 kb on - strand, within Synpcc7942_1565 at 1623.344 kb on - strand, within Synpcc7942_1565 at 1623.348 kb on + strand, within Synpcc7942_1565 at 1623.372 kb on - strand at 1623.401 kb on + strand at 1623.406 kb on + strand at 1623.411 kb on + strand at 1623.415 kb on + strand at 1623.415 kb on + strand at 1623.423 kb on - strand at 1623.446 kb on + strand at 1623.457 kb on - strand at 1623.493 kb on + strand at 1623.591 kb on + strand at 1623.622 kb on + strand at 1623.651 kb on + strand at 1623.667 kb on - strand at 1623.736 kb on + strand, within ppk at 1623.771 kb on + strand, within ppk at 1623.810 kb on + strand, within ppk at 1623.815 kb on + strand, within ppk at 1623.871 kb on + strand, within ppk at 1623.901 kb on + strand, within ppk at 1623.950 kb on - strand, within ppk at 1623.972 kb on + strand, within ppk at 1623.991 kb on - strand, within ppk at 1624.025 kb on - strand, within ppk at 1624.262 kb on - strand, within ppk at 1624.275 kb on + strand, within ppk at 1624.305 kb on - strand, within ppk at 1624.310 kb on - strand, within ppk at 1624.354 kb on - strand, within ppk at 1624.381 kb on - strand, within ppk at 1624.397 kb on + strand, within ppk at 1624.543 kb on - strand, within ppk at 1624.545 kb on + strand, within ppk at 1624.553 kb on - strand, within ppk at 1624.553 kb on - strand, within ppk at 1624.553 kb on - strand, within ppk at 1624.560 kb on - strand at 1624.560 kb on - strand, within ppk at 1624.575 kb on + strand, within ppk at 1624.611 kb on - strand, within ppk at 1624.623 kb on - strand, within ppk at 1624.655 kb on + strand, within ppk at 1624.656 kb on - strand, within ppk at 1624.710 kb on - strand, within ppk at 1624.737 kb on + strand, within ppk at 1624.794 kb on - strand, within ppk at 1624.802 kb on + strand, within ppk at 1624.824 kb on - strand, within ppk at 1624.859 kb on + strand, within ppk at 1624.867 kb on - strand, within ppk at 1624.867 kb on - strand, within ppk at 1624.874 kb on - strand, within ppk at 1624.896 kb on - strand, within ppk at 1624.898 kb on + strand, within ppk at 1624.940 kb on + strand, within ppk at 1624.974 kb on - strand, within ppk at 1624.989 kb on - strand, within ppk at 1624.991 kb on + strand, within ppk at 1624.999 kb on - strand, within ppk at 1625.117 kb on - strand at 1625.229 kb on - strand, within ppk at 1625.238 kb on - strand, within ppk at 1625.240 kb on + strand, within ppk at 1625.286 kb on + strand, within ppk at 1625.286 kb on - strand, within ppk at 1625.319 kb on - strand, within ppk at 1625.428 kb on + strand at 1625.429 kb on - strand at 1625.433 kb on + strand at 1625.434 kb on - strand at 1625.492 kb on - strand at 1625.550 kb on + strand at 1625.553 kb on + strand at 1625.556 kb on - strand at 1625.563 kb on - strand at 1625.602 kb on - strand at 1625.630 kb on + strand at 1625.638 kb on - strand at 1625.651 kb on + strand at 1625.656 kb on + strand at 1625.656 kb on + strand at 1625.664 kb on - strand at 1625.672 kb on - strand at 1625.680 kb on + strand at 1625.771 kb on - strand, within Synpcc7942_1567 at 1625.795 kb on - strand, within Synpcc7942_1567 at 1625.821 kb on + strand, within Synpcc7942_1567 at 1625.825 kb on + strand, within Synpcc7942_1567 at 1625.842 kb on + strand, within Synpcc7942_1567 at 1625.850 kb on - strand, within Synpcc7942_1567 at 1625.850 kb on - strand, within Synpcc7942_1567 at 1625.968 kb on - strand, within Synpcc7942_1567 at 1626.019 kb on - strand at 1626.049 kb on + strand at 1626.091 kb on - strand at 1626.091 kb on - strand at 1626.092 kb on + strand at 1626.092 kb on + strand at 1626.092 kb on + strand at 1626.100 kb on - strand at 1626.100 kb on - strand at 1626.100 kb on - strand at 1626.100 kb on - strand at 1626.135 kb on - strand at 1626.135 kb on - strand at 1626.152 kb on + strand at 1626.160 kb on - strand at 1626.160 kb on - strand at 1626.179 kb on - strand at 1626.204 kb on + strand at 1626.227 kb on - strand at 1626.251 kb on + strand at 1626.251 kb on + strand at 1626.259 kb on - strand at 1626.264 kb on - strand at 1626.276 kb on + strand at 1626.305 kb on + strand at 1626.318 kb on - strand at 1626.323 kb on + strand at 1626.366 kb on - strand at 1626.391 kb on + strand, within Synpcc7942_1568 at 1626.391 kb on + strand, within Synpcc7942_1568 at 1626.394 kb on - strand, within Synpcc7942_1568 at 1626.399 kb on - strand, within Synpcc7942_1568 at 1626.399 kb on - strand, within Synpcc7942_1568 at 1626.403 kb on - strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.607 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,622,468 + -0.8 1,622,477 - +3.6 1,622,869 + +1.9 1,622,877 - +0.1 1,622,919 + -2.0 1,622,919 + +0.8 1,622,921 + -1.6 1,622,931 + -2.6 1,622,950 - +0.8 1,622,960 - -1.8 1,622,966 - -4.3 1,622,987 - -0.7 1,622,991 + -2.5 1,622,999 - -1.7 1,623,038 + Synpcc7942_1565 0.15 -1.4 1,623,102 + Synpcc7942_1565 0.30 -0.7 1,623,114 - Synpcc7942_1565 0.33 -2.2 1,623,146 - Synpcc7942_1565 0.40 -0.9 1,623,255 + Synpcc7942_1565 0.65 -1.1 1,623,265 - Synpcc7942_1565 0.67 -1.1 1,623,270 - Synpcc7942_1565 0.68 -0.5 1,623,329 - Synpcc7942_1565 0.81 -1.6 1,623,344 - Synpcc7942_1565 0.85 -0.9 1,623,348 + Synpcc7942_1565 0.86 -1.4 1,623,372 - -1.2 1,623,401 + +0.5 1,623,406 + -0.8 1,623,411 + -1.4 1,623,415 + -3.1 1,623,415 + -0.7 1,623,423 - +1.4 1,623,446 + -2.0 1,623,457 - -1.9 1,623,493 + -0.4 1,623,591 + -2.4 1,623,622 + -1.8 1,623,651 + -1.2 1,623,667 - -1.7 1,623,736 + ppk Synpcc7942_1566 0.13 -2.5 1,623,771 + ppk Synpcc7942_1566 0.15 -1.8 1,623,810 + ppk Synpcc7942_1566 0.16 -0.9 1,623,815 + ppk Synpcc7942_1566 0.17 -3.2 1,623,871 + ppk Synpcc7942_1566 0.19 -0.0 1,623,901 + ppk Synpcc7942_1566 0.21 -2.1 1,623,950 - ppk Synpcc7942_1566 0.23 +0.1 1,623,972 + ppk Synpcc7942_1566 0.24 -1.4 1,623,991 - ppk Synpcc7942_1566 0.25 -1.4 1,624,025 - ppk Synpcc7942_1566 0.26 -1.5 1,624,262 - ppk Synpcc7942_1566 0.37 -4.5 1,624,275 + ppk Synpcc7942_1566 0.38 +2.1 1,624,305 - ppk Synpcc7942_1566 0.39 -4.3 1,624,310 - ppk Synpcc7942_1566 0.39 -2.0 1,624,354 - ppk Synpcc7942_1566 0.41 -1.5 1,624,381 - ppk Synpcc7942_1566 0.42 -1.9 1,624,397 + ppk Synpcc7942_1566 0.43 -3.1 1,624,543 - ppk Synpcc7942_1566 0.50 -3.4 1,624,545 + ppk Synpcc7942_1566 0.50 -1.7 1,624,553 - ppk Synpcc7942_1566 0.50 -3.2 1,624,553 - ppk Synpcc7942_1566 0.50 -0.7 1,624,553 - ppk Synpcc7942_1566 0.50 -2.5 1,624,560 - -1.2 1,624,560 - ppk Synpcc7942_1566 0.51 -4.6 1,624,575 + ppk Synpcc7942_1566 0.51 -1.4 1,624,611 - ppk Synpcc7942_1566 0.53 -2.7 1,624,623 - ppk Synpcc7942_1566 0.53 -3.4 1,624,655 + ppk Synpcc7942_1566 0.55 -1.7 1,624,656 - ppk Synpcc7942_1566 0.55 -3.5 1,624,710 - ppk Synpcc7942_1566 0.57 -1.7 1,624,737 + ppk Synpcc7942_1566 0.59 -1.5 1,624,794 - ppk Synpcc7942_1566 0.61 -1.5 1,624,802 + ppk Synpcc7942_1566 0.62 -1.1 1,624,824 - ppk Synpcc7942_1566 0.63 -2.1 1,624,859 + ppk Synpcc7942_1566 0.64 -1.7 1,624,867 - ppk Synpcc7942_1566 0.65 -2.2 1,624,867 - ppk Synpcc7942_1566 0.65 -3.2 1,624,874 - ppk Synpcc7942_1566 0.65 -2.7 1,624,896 - ppk Synpcc7942_1566 0.66 -2.2 1,624,898 + ppk Synpcc7942_1566 0.66 -2.3 1,624,940 + ppk Synpcc7942_1566 0.68 -4.2 1,624,974 - ppk Synpcc7942_1566 0.69 -2.0 1,624,989 - ppk Synpcc7942_1566 0.70 -2.3 1,624,991 + ppk Synpcc7942_1566 0.70 +1.9 1,624,999 - ppk Synpcc7942_1566 0.71 -3.3 1,625,117 - -1.7 1,625,229 - ppk Synpcc7942_1566 0.81 +0.2 1,625,238 - ppk Synpcc7942_1566 0.81 -3.3 1,625,240 + ppk Synpcc7942_1566 0.82 -3.1 1,625,286 + ppk Synpcc7942_1566 0.84 -1.1 1,625,286 - ppk Synpcc7942_1566 0.84 -2.6 1,625,319 - ppk Synpcc7942_1566 0.85 -1.5 1,625,428 + -0.6 1,625,429 - -1.8 1,625,433 + -3.8 1,625,434 - +1.5 1,625,492 - -2.0 1,625,550 + -1.9 1,625,553 + -1.8 1,625,556 - -1.2 1,625,563 - -1.1 1,625,602 - -1.9 1,625,630 + -0.4 1,625,638 - -2.5 1,625,651 + +0.1 1,625,656 + +0.8 1,625,656 + +1.4 1,625,664 - -1.5 1,625,672 - +0.1 1,625,680 + -0.2 1,625,771 - Synpcc7942_1567 0.22 -0.9 1,625,795 - Synpcc7942_1567 0.29 -0.2 1,625,821 + Synpcc7942_1567 0.37 -0.3 1,625,825 + Synpcc7942_1567 0.39 -2.9 1,625,842 + Synpcc7942_1567 0.44 +1.6 1,625,850 - Synpcc7942_1567 0.47 +1.0 1,625,850 - Synpcc7942_1567 0.47 -2.6 1,625,968 - Synpcc7942_1567 0.84 +2.4 1,626,019 - +2.1 1,626,049 + +3.7 1,626,091 - -1.1 1,626,091 - +1.3 1,626,092 + +0.4 1,626,092 + +2.6 1,626,092 + -1.8 1,626,100 - +1.5 1,626,100 - +1.0 1,626,100 - +3.4 1,626,100 - +2.5 1,626,135 - -0.2 1,626,135 - +1.6 1,626,152 + -2.9 1,626,160 - -2.9 1,626,160 - +0.7 1,626,179 - +1.1 1,626,204 + -1.7 1,626,227 - -0.6 1,626,251 + +3.0 1,626,251 + +1.1 1,626,259 - -0.2 1,626,264 - +0.9 1,626,276 + +1.3 1,626,305 + +0.8 1,626,318 - -1.1 1,626,323 + +1.4 1,626,366 - -2.2 1,626,391 + Synpcc7942_1568 0.14 +3.3 1,626,391 + Synpcc7942_1568 0.14 -2.1 1,626,394 - Synpcc7942_1568 0.15 +2.8 1,626,399 - Synpcc7942_1568 0.17 +1.4 1,626,399 - Synpcc7942_1568 0.17 -1.9 1,626,403 - Synpcc7942_1568 0.19 -2.5 1,626,518 + Synpcc7942_1568 0.67 +2.8 1,626,518 + Synpcc7942_1568 0.67 -0.4 1,626,518 + Synpcc7942_1568 0.67 -2.3 1,626,518 + Synpcc7942_1568 0.67 -0.2 1,626,518 + Synpcc7942_1568 0.67 +0.8 1,626,518 + Synpcc7942_1568 0.67 +4.1 1,626,518 + Synpcc7942_1568 0.67 +1.1 1,626,518 + Synpcc7942_1568 0.67 +0.1 1,626,518 + Synpcc7942_1568 0.67 -1.6 1,626,518 + Synpcc7942_1568 0.67 +2.9 1,626,526 - Synpcc7942_1568 0.70 -1.9 1,626,526 - Synpcc7942_1568 0.70 +0.7 1,626,526 - Synpcc7942_1568 0.70 -1.2 1,626,526 - Synpcc7942_1568 0.70 -0.4 1,626,526 - Synpcc7942_1568 0.70 +1.8 1,626,526 - Synpcc7942_1568 0.70 +0.1 1,626,526 - Synpcc7942_1568 0.70 +2.0 1,626,526 - Synpcc7942_1568 0.70 -0.4 1,626,526 - Synpcc7942_1568 0.70 -1.6 1,626,526 - Synpcc7942_1568 0.70 -1.2 1,626,526 - Synpcc7942_1568 0.70 -1.4 1,626,526 - Synpcc7942_1568 0.70 +2.0 1,626,526 - Synpcc7942_1568 0.70 -1.6 1,626,607 + -1.1
Or see this region's nucleotide sequence