Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1564 and tRNA-Gly are separated by 577 nucleotides tRNA-Gly and Synpcc7942_1565 are separated by 100 nucleotides Synpcc7942_1565 and ppk are separated by 38 nucleotides
Synpcc7942_1564: Synpcc7942_1564 - hypothetical protein, at 1,621,903 to 1,622,220
_1564
Synpcc7942_R0001: tRNA-Gly - tRNA-Gly, at 1,622,798 to 1,622,869
tRNA-Gly
Synpcc7942_1565: Synpcc7942_1565 - hypothetical protein, at 1,622,970 to 1,623,410
_1565
Synpcc7942_1566: ppk - polyphosphate kinase, at 1,623,449 to 1,625,644
ppk
Position (kb)
1622
1623
1624 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1621.985 kb on + strand, within Synpcc7942_1564 at 1621.985 kb on + strand, within Synpcc7942_1564 at 1621.999 kb on - strand, within Synpcc7942_1564 at 1622.008 kb on + strand, within Synpcc7942_1564 at 1622.016 kb on + strand, within Synpcc7942_1564 at 1622.028 kb on + strand, within Synpcc7942_1564 at 1622.028 kb on + strand, within Synpcc7942_1564 at 1622.028 kb on + strand, within Synpcc7942_1564 at 1622.030 kb on - strand, within Synpcc7942_1564 at 1622.036 kb on - strand, within Synpcc7942_1564 at 1622.036 kb on - strand, within Synpcc7942_1564 at 1622.036 kb on - strand, within Synpcc7942_1564 at 1622.071 kb on - strand, within Synpcc7942_1564 at 1622.104 kb on + strand, within Synpcc7942_1564 at 1622.113 kb on - strand, within Synpcc7942_1564 at 1622.124 kb on - strand, within Synpcc7942_1564 at 1622.132 kb on + strand, within Synpcc7942_1564 at 1622.132 kb on + strand, within Synpcc7942_1564 at 1622.160 kb on - strand, within Synpcc7942_1564 at 1622.225 kb on - strand at 1622.235 kb on - strand at 1622.408 kb on - strand at 1622.468 kb on + strand at 1622.477 kb on - strand at 1622.869 kb on + strand at 1622.877 kb on - strand at 1622.919 kb on + strand at 1622.919 kb on + strand at 1622.921 kb on + strand at 1622.931 kb on + strand at 1622.950 kb on - strand at 1622.960 kb on - strand at 1622.966 kb on - strand at 1622.987 kb on - strand at 1622.991 kb on + strand at 1622.999 kb on - strand at 1623.038 kb on + strand, within Synpcc7942_1565 at 1623.102 kb on + strand, within Synpcc7942_1565 at 1623.114 kb on - strand, within Synpcc7942_1565 at 1623.146 kb on - strand, within Synpcc7942_1565 at 1623.255 kb on + strand, within Synpcc7942_1565 at 1623.265 kb on - strand, within Synpcc7942_1565 at 1623.270 kb on - strand, within Synpcc7942_1565 at 1623.329 kb on - strand, within Synpcc7942_1565 at 1623.344 kb on - strand, within Synpcc7942_1565 at 1623.348 kb on + strand, within Synpcc7942_1565 at 1623.372 kb on - strand at 1623.401 kb on + strand at 1623.406 kb on + strand at 1623.411 kb on + strand at 1623.415 kb on + strand at 1623.415 kb on + strand at 1623.423 kb on - strand at 1623.446 kb on + strand at 1623.457 kb on - strand at 1623.493 kb on + strand at 1623.591 kb on + strand at 1623.622 kb on + strand at 1623.651 kb on + strand at 1623.667 kb on - strand at 1623.736 kb on + strand, within ppk at 1623.771 kb on + strand, within ppk at 1623.810 kb on + strand, within ppk at 1623.815 kb on + strand, within ppk at 1623.871 kb on + strand, within ppk at 1623.901 kb on + strand, within ppk at 1623.950 kb on - strand, within ppk at 1623.972 kb on + strand, within ppk at 1623.991 kb on - strand, within ppk at 1624.025 kb on - strand, within ppk at 1624.262 kb on - strand, within ppk at 1624.275 kb on + strand, within ppk at 1624.305 kb on - strand, within ppk at 1624.310 kb on - strand, within ppk at 1624.354 kb on - strand, within ppk at 1624.381 kb on - strand, within ppk at 1624.397 kb on + strand, within ppk
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,621,985 + Synpcc7942_1564 0.26 +1.9 1,621,985 + Synpcc7942_1564 0.26 +1.2 1,621,999 - Synpcc7942_1564 0.30 -1.5 1,622,008 + Synpcc7942_1564 0.33 -2.0 1,622,016 + Synpcc7942_1564 0.36 +0.6 1,622,028 + Synpcc7942_1564 0.39 +0.3 1,622,028 + Synpcc7942_1564 0.39 -0.6 1,622,028 + Synpcc7942_1564 0.39 +2.1 1,622,030 - Synpcc7942_1564 0.40 -0.2 1,622,036 - Synpcc7942_1564 0.42 +2.1 1,622,036 - Synpcc7942_1564 0.42 +2.2 1,622,036 - Synpcc7942_1564 0.42 +0.0 1,622,071 - Synpcc7942_1564 0.53 -2.0 1,622,104 + Synpcc7942_1564 0.63 -0.7 1,622,113 - Synpcc7942_1564 0.66 -0.5 1,622,124 - Synpcc7942_1564 0.69 +1.6 1,622,132 + Synpcc7942_1564 0.72 +0.2 1,622,132 + Synpcc7942_1564 0.72 -0.4 1,622,160 - Synpcc7942_1564 0.81 -0.2 1,622,225 - +2.0 1,622,235 - -0.2 1,622,408 - -1.2 1,622,468 + -0.8 1,622,477 - +3.6 1,622,869 + +1.9 1,622,877 - +0.1 1,622,919 + -2.0 1,622,919 + +0.8 1,622,921 + -1.6 1,622,931 + -2.6 1,622,950 - +0.8 1,622,960 - -1.8 1,622,966 - -4.3 1,622,987 - -0.7 1,622,991 + -2.5 1,622,999 - -1.7 1,623,038 + Synpcc7942_1565 0.15 -1.4 1,623,102 + Synpcc7942_1565 0.30 -0.7 1,623,114 - Synpcc7942_1565 0.33 -2.2 1,623,146 - Synpcc7942_1565 0.40 -0.9 1,623,255 + Synpcc7942_1565 0.65 -1.1 1,623,265 - Synpcc7942_1565 0.67 -1.1 1,623,270 - Synpcc7942_1565 0.68 -0.5 1,623,329 - Synpcc7942_1565 0.81 -1.6 1,623,344 - Synpcc7942_1565 0.85 -0.9 1,623,348 + Synpcc7942_1565 0.86 -1.4 1,623,372 - -1.2 1,623,401 + +0.5 1,623,406 + -0.8 1,623,411 + -1.4 1,623,415 + -3.1 1,623,415 + -0.7 1,623,423 - +1.4 1,623,446 + -2.0 1,623,457 - -1.9 1,623,493 + -0.4 1,623,591 + -2.4 1,623,622 + -1.8 1,623,651 + -1.2 1,623,667 - -1.7 1,623,736 + ppk Synpcc7942_1566 0.13 -2.5 1,623,771 + ppk Synpcc7942_1566 0.15 -1.8 1,623,810 + ppk Synpcc7942_1566 0.16 -0.9 1,623,815 + ppk Synpcc7942_1566 0.17 -3.2 1,623,871 + ppk Synpcc7942_1566 0.19 -0.0 1,623,901 + ppk Synpcc7942_1566 0.21 -2.1 1,623,950 - ppk Synpcc7942_1566 0.23 +0.1 1,623,972 + ppk Synpcc7942_1566 0.24 -1.4 1,623,991 - ppk Synpcc7942_1566 0.25 -1.4 1,624,025 - ppk Synpcc7942_1566 0.26 -1.5 1,624,262 - ppk Synpcc7942_1566 0.37 -4.5 1,624,275 + ppk Synpcc7942_1566 0.38 +2.1 1,624,305 - ppk Synpcc7942_1566 0.39 -4.3 1,624,310 - ppk Synpcc7942_1566 0.39 -2.0 1,624,354 - ppk Synpcc7942_1566 0.41 -1.5 1,624,381 - ppk Synpcc7942_1566 0.42 -1.9 1,624,397 + ppk Synpcc7942_1566 0.43 -3.1
Or see this region's nucleotide sequence