Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1532 and sdhB overlap by 23 nucleotides sdhB and Synpcc7942_1534 are separated by 121 nucleotides Synpcc7942_1534 and rbcX are separated by 63 nucleotides rbcX and gat overlap by 11 nucleotides
Synpcc7942_1532: Synpcc7942_1532 - Molybdate ABC transporter, permease protein, at 1,591,611 to 1,592,276
_1532
Synpcc7942_1533: sdhB - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 1,592,254 to 1,593,093
sdhB
Synpcc7942_1534: Synpcc7942_1534 - hypothetical protein, at 1,593,215 to 1,595,422
_1534
Synpcc7942_1535: rbcX - possible Rubisco chaperonin, at 1,595,486 to 1,595,944
rbcX
Synpcc7942_1536: gat - probable amidotransferase, at 1,595,934 to 1,596,614
gat
Position (kb)
1593
1594
1595
1596 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1592.230 kb on - strand at 1592.249 kb on + strand at 1592.249 kb on + strand at 1592.271 kb on + strand at 1592.314 kb on - strand at 1592.327 kb on + strand at 1592.327 kb on + strand at 1592.335 kb on - strand at 1592.342 kb on - strand, within sdhB at 1592.390 kb on + strand, within sdhB at 1592.396 kb on - strand, within sdhB at 1592.398 kb on - strand, within sdhB at 1592.400 kb on + strand, within sdhB at 1592.400 kb on + strand, within sdhB at 1592.408 kb on + strand, within sdhB at 1592.408 kb on - strand, within sdhB at 1592.419 kb on + strand, within sdhB at 1592.445 kb on + strand, within sdhB at 1592.451 kb on - strand, within sdhB at 1592.460 kb on - strand, within sdhB at 1592.485 kb on - strand, within sdhB at 1592.489 kb on + strand, within sdhB at 1592.497 kb on - strand, within sdhB at 1592.551 kb on - strand, within sdhB at 1592.560 kb on + strand, within sdhB at 1592.596 kb on + strand, within sdhB at 1592.602 kb on + strand, within sdhB at 1592.647 kb on - strand, within sdhB at 1592.664 kb on - strand, within sdhB at 1592.664 kb on - strand, within sdhB at 1592.674 kb on + strand, within sdhB at 1592.674 kb on + strand at 1592.681 kb on - strand, within sdhB at 1592.682 kb on - strand, within sdhB at 1592.721 kb on + strand, within sdhB at 1592.728 kb on + strand, within sdhB at 1592.733 kb on - strand, within sdhB at 1592.733 kb on - strand, within sdhB at 1592.795 kb on - strand, within sdhB at 1592.848 kb on - strand, within sdhB at 1592.950 kb on - strand, within sdhB at 1592.961 kb on + strand, within sdhB at 1592.982 kb on - strand, within sdhB at 1592.982 kb on - strand, within sdhB at 1593.037 kb on + strand at 1593.042 kb on + strand at 1593.055 kb on - strand at 1593.096 kb on - strand at 1593.123 kb on + strand at 1593.123 kb on + strand at 1593.140 kb on + strand at 1593.171 kb on + strand at 1593.171 kb on + strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.179 kb on - strand at 1593.189 kb on - strand at 1593.189 kb on - strand at 1593.218 kb on + strand at 1593.459 kb on + strand, within Synpcc7942_1534 at 1593.514 kb on + strand, within Synpcc7942_1534 at 1593.576 kb on + strand, within Synpcc7942_1534 at 1593.628 kb on + strand, within Synpcc7942_1534 at 1593.655 kb on + strand, within Synpcc7942_1534 at 1593.742 kb on + strand, within Synpcc7942_1534 at 1593.742 kb on + strand, within Synpcc7942_1534 at 1593.750 kb on - strand, within Synpcc7942_1534 at 1593.809 kb on - strand, within Synpcc7942_1534 at 1593.824 kb on + strand, within Synpcc7942_1534 at 1593.861 kb on + strand, within Synpcc7942_1534 at 1594.088 kb on + strand, within Synpcc7942_1534 at 1594.088 kb on + strand, within Synpcc7942_1534 at 1594.094 kb on - strand, within Synpcc7942_1534 at 1594.121 kb on + strand, within Synpcc7942_1534 at 1594.143 kb on + strand, within Synpcc7942_1534 at 1594.151 kb on - strand, within Synpcc7942_1534 at 1594.151 kb on - strand, within Synpcc7942_1534 at 1594.166 kb on + strand, within Synpcc7942_1534 at 1594.166 kb on + strand, within Synpcc7942_1534 at 1594.181 kb on + strand, within Synpcc7942_1534 at 1594.215 kb on + strand, within Synpcc7942_1534 at 1594.257 kb on - strand, within Synpcc7942_1534 at 1594.291 kb on + strand, within Synpcc7942_1534 at 1594.297 kb on - strand, within Synpcc7942_1534 at 1594.338 kb on - strand, within Synpcc7942_1534 at 1594.475 kb on + strand, within Synpcc7942_1534 at 1594.483 kb on - strand, within Synpcc7942_1534 at 1594.485 kb on + strand, within Synpcc7942_1534 at 1594.485 kb on + strand at 1594.619 kb on - strand, within Synpcc7942_1534 at 1594.781 kb on - strand, within Synpcc7942_1534 at 1594.841 kb on - strand, within Synpcc7942_1534 at 1594.848 kb on + strand, within Synpcc7942_1534 at 1594.850 kb on + strand, within Synpcc7942_1534 at 1594.882 kb on - strand at 1594.938 kb on + strand, within Synpcc7942_1534 at 1594.940 kb on + strand, within Synpcc7942_1534 at 1594.976 kb on - strand, within Synpcc7942_1534 at 1595.015 kb on - strand, within Synpcc7942_1534 at 1595.051 kb on - strand, within Synpcc7942_1534 at 1595.135 kb on - strand, within Synpcc7942_1534 at 1595.178 kb on - strand, within Synpcc7942_1534 at 1595.233 kb on - strand at 1595.337 kb on - strand at 1595.439 kb on - strand at 1595.477 kb on + strand at 1595.477 kb on + strand at 1595.485 kb on - strand at 1595.498 kb on - strand at 1595.530 kb on - strand at 1595.532 kb on + strand, within rbcX at 1595.576 kb on - strand, within rbcX at 1595.597 kb on + strand, within rbcX at 1595.622 kb on + strand, within rbcX at 1595.629 kb on - strand, within rbcX at 1595.666 kb on - strand, within rbcX at 1595.690 kb on + strand, within rbcX at 1595.700 kb on - strand, within rbcX at 1595.709 kb on + strand, within rbcX at 1595.722 kb on + strand, within rbcX at 1595.722 kb on + strand, within rbcX at 1595.730 kb on - strand, within rbcX at 1595.749 kb on - strand, within rbcX at 1595.791 kb on + strand, within rbcX at 1595.801 kb on - strand, within rbcX at 1595.844 kb on + strand, within rbcX at 1595.850 kb on + strand, within rbcX at 1595.911 kb on - strand at 1595.915 kb on - strand at 1595.971 kb on + strand at 1596.007 kb on + strand, within gat at 1596.019 kb on + strand, within gat at 1596.019 kb on + strand, within gat at 1596.027 kb on - strand, within gat at 1596.080 kb on + strand, within gat at 1596.095 kb on + strand, within gat at 1596.121 kb on + strand, within gat at 1596.129 kb on - strand, within gat at 1596.221 kb on + strand, within gat at 1596.221 kb on + strand at 1596.222 kb on - strand, within gat at 1596.222 kb on - strand, within gat at 1596.222 kb on - strand, within gat at 1596.273 kb on + strand, within gat at 1596.286 kb on - strand, within gat at 1596.343 kb on + strand, within gat at 1596.377 kb on - strand, within gat at 1596.421 kb on - strand, within gat
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,592,230 - -1.4 1,592,249 + -1.2 1,592,249 + -0.7 1,592,271 + -1.4 1,592,314 - -0.5 1,592,327 + +2.1 1,592,327 + +3.8 1,592,335 - -2.3 1,592,342 - sdhB Synpcc7942_1533 0.10 +0.5 1,592,390 + sdhB Synpcc7942_1533 0.16 -2.1 1,592,396 - sdhB Synpcc7942_1533 0.17 +0.4 1,592,398 - sdhB Synpcc7942_1533 0.17 -0.3 1,592,400 + sdhB Synpcc7942_1533 0.17 -0.6 1,592,400 + sdhB Synpcc7942_1533 0.17 -1.8 1,592,408 + sdhB Synpcc7942_1533 0.18 +2.2 1,592,408 - sdhB Synpcc7942_1533 0.18 +1.0 1,592,419 + sdhB Synpcc7942_1533 0.20 +4.4 1,592,445 + sdhB Synpcc7942_1533 0.23 +2.9 1,592,451 - sdhB Synpcc7942_1533 0.23 -2.1 1,592,460 - sdhB Synpcc7942_1533 0.25 -2.2 1,592,485 - sdhB Synpcc7942_1533 0.28 -2.2 1,592,489 + sdhB Synpcc7942_1533 0.28 +1.1 1,592,497 - sdhB Synpcc7942_1533 0.29 -0.8 1,592,551 - sdhB Synpcc7942_1533 0.35 +3.6 1,592,560 + sdhB Synpcc7942_1533 0.36 -1.5 1,592,596 + sdhB Synpcc7942_1533 0.41 -1.3 1,592,602 + sdhB Synpcc7942_1533 0.41 +0.2 1,592,647 - sdhB Synpcc7942_1533 0.47 -0.4 1,592,664 - sdhB Synpcc7942_1533 0.49 -0.0 1,592,664 - sdhB Synpcc7942_1533 0.49 -0.9 1,592,674 + sdhB Synpcc7942_1533 0.50 +0.5 1,592,674 + -1.4 1,592,681 - sdhB Synpcc7942_1533 0.51 -1.9 1,592,682 - sdhB Synpcc7942_1533 0.51 +2.0 1,592,721 + sdhB Synpcc7942_1533 0.56 -0.7 1,592,728 + sdhB Synpcc7942_1533 0.56 -0.0 1,592,733 - sdhB Synpcc7942_1533 0.57 +1.4 1,592,733 - sdhB Synpcc7942_1533 0.57 +0.8 1,592,795 - sdhB Synpcc7942_1533 0.64 +0.4 1,592,848 - sdhB Synpcc7942_1533 0.71 -1.3 1,592,950 - sdhB Synpcc7942_1533 0.83 -1.4 1,592,961 + sdhB Synpcc7942_1533 0.84 +0.7 1,592,982 - sdhB Synpcc7942_1533 0.87 +1.7 1,592,982 - sdhB Synpcc7942_1533 0.87 -1.9 1,593,037 + +2.2 1,593,042 + -2.8 1,593,055 - +0.9 1,593,096 - +1.4 1,593,123 + +0.1 1,593,123 + +0.9 1,593,140 + +1.2 1,593,171 + +0.3 1,593,171 + +2.8 1,593,179 - +0.3 1,593,179 - -3.5 1,593,179 - +3.1 1,593,179 - +0.6 1,593,179 - +3.1 1,593,179 - +0.3 1,593,189 - +2.3 1,593,189 - -0.3 1,593,218 + +1.8 1,593,459 + Synpcc7942_1534 0.11 -1.5 1,593,514 + Synpcc7942_1534 0.14 +4.5 1,593,576 + Synpcc7942_1534 0.16 +1.9 1,593,628 + Synpcc7942_1534 0.19 +2.0 1,593,655 + Synpcc7942_1534 0.20 +1.7 1,593,742 + Synpcc7942_1534 0.24 +0.6 1,593,742 + Synpcc7942_1534 0.24 -0.1 1,593,750 - Synpcc7942_1534 0.24 -3.0 1,593,809 - Synpcc7942_1534 0.27 +0.3 1,593,824 + Synpcc7942_1534 0.28 -2.1 1,593,861 + Synpcc7942_1534 0.29 +1.9 1,594,088 + Synpcc7942_1534 0.40 -3.0 1,594,088 + Synpcc7942_1534 0.40 -1.4 1,594,094 - Synpcc7942_1534 0.40 +1.0 1,594,121 + Synpcc7942_1534 0.41 -1.9 1,594,143 + Synpcc7942_1534 0.42 +2.3 1,594,151 - Synpcc7942_1534 0.42 +1.0 1,594,151 - Synpcc7942_1534 0.42 +2.7 1,594,166 + Synpcc7942_1534 0.43 +0.8 1,594,166 + Synpcc7942_1534 0.43 -2.3 1,594,181 + Synpcc7942_1534 0.44 +2.1 1,594,215 + Synpcc7942_1534 0.45 -0.0 1,594,257 - Synpcc7942_1534 0.47 +1.2 1,594,291 + Synpcc7942_1534 0.49 +1.6 1,594,297 - Synpcc7942_1534 0.49 -1.2 1,594,338 - Synpcc7942_1534 0.51 +1.9 1,594,475 + Synpcc7942_1534 0.57 -2.7 1,594,483 - Synpcc7942_1534 0.57 -2.1 1,594,485 + Synpcc7942_1534 0.58 -2.3 1,594,485 + +1.1 1,594,619 - Synpcc7942_1534 0.64 +1.3 1,594,781 - Synpcc7942_1534 0.71 -0.5 1,594,841 - Synpcc7942_1534 0.74 +0.7 1,594,848 + Synpcc7942_1534 0.74 +2.3 1,594,850 + Synpcc7942_1534 0.74 +5.3 1,594,882 - -1.6 1,594,938 + Synpcc7942_1534 0.78 -2.7 1,594,940 + Synpcc7942_1534 0.78 +3.3 1,594,976 - Synpcc7942_1534 0.80 +2.5 1,595,015 - Synpcc7942_1534 0.82 -2.7 1,595,051 - Synpcc7942_1534 0.83 -0.3 1,595,135 - Synpcc7942_1534 0.87 +0.6 1,595,178 - Synpcc7942_1534 0.89 +1.2 1,595,233 - +2.3 1,595,337 - -0.1 1,595,439 - -0.4 1,595,477 + +2.0 1,595,477 + +0.5 1,595,485 - -0.7 1,595,498 - -0.2 1,595,530 - +0.3 1,595,532 + rbcX Synpcc7942_1535 0.10 +0.6 1,595,576 - rbcX Synpcc7942_1535 0.20 -0.6 1,595,597 + rbcX Synpcc7942_1535 0.24 -1.6 1,595,622 + rbcX Synpcc7942_1535 0.30 +0.6 1,595,629 - rbcX Synpcc7942_1535 0.31 -0.9 1,595,666 - rbcX Synpcc7942_1535 0.39 -1.5 1,595,690 + rbcX Synpcc7942_1535 0.44 +1.5 1,595,700 - rbcX Synpcc7942_1535 0.47 +1.4 1,595,709 + rbcX Synpcc7942_1535 0.49 -1.0 1,595,722 + rbcX Synpcc7942_1535 0.51 -2.9 1,595,722 + rbcX Synpcc7942_1535 0.51 -0.9 1,595,730 - rbcX Synpcc7942_1535 0.53 +1.0 1,595,749 - rbcX Synpcc7942_1535 0.57 +1.1 1,595,791 + rbcX Synpcc7942_1535 0.66 -2.2 1,595,801 - rbcX Synpcc7942_1535 0.69 -0.5 1,595,844 + rbcX Synpcc7942_1535 0.78 -0.5 1,595,850 + rbcX Synpcc7942_1535 0.79 +2.0 1,595,911 - -0.0 1,595,915 - -0.2 1,595,971 + -1.4 1,596,007 + gat Synpcc7942_1536 0.11 +1.0 1,596,019 + gat Synpcc7942_1536 0.12 -0.9 1,596,019 + gat Synpcc7942_1536 0.12 -1.6 1,596,027 - gat Synpcc7942_1536 0.14 +0.2 1,596,080 + gat Synpcc7942_1536 0.21 -0.3 1,596,095 + gat Synpcc7942_1536 0.24 +0.9 1,596,121 + gat Synpcc7942_1536 0.27 -1.1 1,596,129 - gat Synpcc7942_1536 0.29 -1.9 1,596,221 + gat Synpcc7942_1536 0.42 -1.5 1,596,221 + -0.7 1,596,222 - gat Synpcc7942_1536 0.42 -2.3 1,596,222 - gat Synpcc7942_1536 0.42 -1.3 1,596,222 - gat Synpcc7942_1536 0.42 -3.6 1,596,273 + gat Synpcc7942_1536 0.50 +2.7 1,596,286 - gat Synpcc7942_1536 0.52 -1.2 1,596,343 + gat Synpcc7942_1536 0.60 -1.1 1,596,377 - gat Synpcc7942_1536 0.65 +0.7 1,596,421 - gat Synpcc7942_1536 0.72 -1.2
Or see this region's nucleotide sequence