Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1514 and Synpcc7942_1515 are separated by 41 nucleotides Synpcc7942_1515 and Synpcc7942_1516 are separated by 33 nucleotides Synpcc7942_1516 and Synpcc7942_1517 overlap by 29 nucleotides
Synpcc7942_1514: Synpcc7942_1514 - Pseudouridine synthase, Rsu, at 1,564,750 to 1,565,349
_1514
Synpcc7942_1515: Synpcc7942_1515 - protein serine/threonine phosphatase, at 1,565,391 to 1,566,290
_1515
Synpcc7942_1516: Synpcc7942_1516 - phosphoglycerate mutase, at 1,566,324 to 1,566,971
_1516
Synpcc7942_1517: Synpcc7942_1517 - histidine kinase, at 1,566,943 to 1,568,316
_1517
Position (kb)
1566
1567 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1565.337 kb on - strand at 1565.357 kb on - strand at 1565.364 kb on - strand at 1565.375 kb on + strand at 1565.383 kb on - strand at 1565.395 kb on - strand at 1565.410 kb on + strand at 1565.411 kb on - strand at 1565.442 kb on - strand at 1565.451 kb on + strand at 1565.452 kb on - strand at 1565.538 kb on + strand, within Synpcc7942_1515 at 1565.551 kb on + strand, within Synpcc7942_1515 at 1565.569 kb on - strand, within Synpcc7942_1515 at 1565.571 kb on + strand, within Synpcc7942_1515 at 1565.594 kb on - strand, within Synpcc7942_1515 at 1565.609 kb on + strand, within Synpcc7942_1515 at 1565.622 kb on - strand, within Synpcc7942_1515 at 1565.629 kb on + strand, within Synpcc7942_1515 at 1565.637 kb on - strand, within Synpcc7942_1515 at 1565.671 kb on + strand, within Synpcc7942_1515 at 1565.701 kb on + strand, within Synpcc7942_1515 at 1565.724 kb on + strand, within Synpcc7942_1515 at 1565.726 kb on + strand, within Synpcc7942_1515 at 1565.732 kb on - strand, within Synpcc7942_1515 at 1565.739 kb on - strand, within Synpcc7942_1515 at 1565.741 kb on + strand, within Synpcc7942_1515 at 1565.752 kb on + strand, within Synpcc7942_1515 at 1565.847 kb on - strand, within Synpcc7942_1515 at 1565.850 kb on + strand, within Synpcc7942_1515 at 1565.850 kb on - strand, within Synpcc7942_1515 at 1565.870 kb on + strand, within Synpcc7942_1515 at 1565.905 kb on - strand, within Synpcc7942_1515 at 1565.939 kb on + strand, within Synpcc7942_1515 at 1565.962 kb on + strand, within Synpcc7942_1515 at 1565.969 kb on - strand, within Synpcc7942_1515 at 1565.973 kb on + strand, within Synpcc7942_1515 at 1565.973 kb on + strand, within Synpcc7942_1515 at 1565.981 kb on - strand, within Synpcc7942_1515 at 1565.981 kb on - strand, within Synpcc7942_1515 at 1566.037 kb on - strand, within Synpcc7942_1515 at 1566.040 kb on + strand, within Synpcc7942_1515 at 1566.042 kb on - strand, within Synpcc7942_1515 at 1566.084 kb on - strand, within Synpcc7942_1515 at 1566.099 kb on - strand, within Synpcc7942_1515 at 1566.135 kb on - strand, within Synpcc7942_1515 at 1566.153 kb on + strand, within Synpcc7942_1515 at 1566.176 kb on + strand, within Synpcc7942_1515 at 1566.176 kb on + strand, within Synpcc7942_1515 at 1566.233 kb on + strand at 1566.240 kb on - strand at 1566.240 kb on - strand at 1566.240 kb on - strand at 1566.241 kb on - strand at 1566.272 kb on + strand at 1566.284 kb on + strand at 1566.313 kb on + strand at 1566.353 kb on + strand at 1566.355 kb on - strand at 1566.490 kb on + strand, within Synpcc7942_1516 at 1566.559 kb on + strand, within Synpcc7942_1516 at 1566.981 kb on + strand at 1566.989 kb on + strand at 1566.989 kb on + strand at 1566.989 kb on + strand at 1566.989 kb on + strand at 1566.997 kb on - strand at 1567.675 kb on - strand, within Synpcc7942_1517
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,565,337 - -2.5 1,565,357 - -2.8 1,565,364 - -0.6 1,565,375 + +0.2 1,565,383 - +0.6 1,565,395 - +2.1 1,565,410 + +0.0 1,565,411 - -3.1 1,565,442 - -2.8 1,565,451 + -0.8 1,565,452 - +2.2 1,565,538 + Synpcc7942_1515 0.16 -0.5 1,565,551 + Synpcc7942_1515 0.18 -1.7 1,565,569 - Synpcc7942_1515 0.20 -0.9 1,565,571 + Synpcc7942_1515 0.20 -2.4 1,565,594 - Synpcc7942_1515 0.23 -4.2 1,565,609 + Synpcc7942_1515 0.24 -3.7 1,565,622 - Synpcc7942_1515 0.26 -2.1 1,565,629 + Synpcc7942_1515 0.26 -2.0 1,565,637 - Synpcc7942_1515 0.27 -0.5 1,565,671 + Synpcc7942_1515 0.31 -1.3 1,565,701 + Synpcc7942_1515 0.34 -3.8 1,565,724 + Synpcc7942_1515 0.37 -2.4 1,565,726 + Synpcc7942_1515 0.37 -1.6 1,565,732 - Synpcc7942_1515 0.38 -1.2 1,565,739 - Synpcc7942_1515 0.39 +2.3 1,565,741 + Synpcc7942_1515 0.39 -2.8 1,565,752 + Synpcc7942_1515 0.40 -2.3 1,565,847 - Synpcc7942_1515 0.51 -2.0 1,565,850 + Synpcc7942_1515 0.51 -2.6 1,565,850 - Synpcc7942_1515 0.51 +0.2 1,565,870 + Synpcc7942_1515 0.53 -0.8 1,565,905 - Synpcc7942_1515 0.57 -4.1 1,565,939 + Synpcc7942_1515 0.61 +0.4 1,565,962 + Synpcc7942_1515 0.63 +0.6 1,565,969 - Synpcc7942_1515 0.64 -0.4 1,565,973 + Synpcc7942_1515 0.65 -0.1 1,565,973 + Synpcc7942_1515 0.65 -1.9 1,565,981 - Synpcc7942_1515 0.66 -1.1 1,565,981 - Synpcc7942_1515 0.66 -2.5 1,566,037 - Synpcc7942_1515 0.72 -3.1 1,566,040 + Synpcc7942_1515 0.72 -1.9 1,566,042 - Synpcc7942_1515 0.72 -3.8 1,566,084 - Synpcc7942_1515 0.77 -1.7 1,566,099 - Synpcc7942_1515 0.79 -1.3 1,566,135 - Synpcc7942_1515 0.83 +2.4 1,566,153 + Synpcc7942_1515 0.85 -2.5 1,566,176 + Synpcc7942_1515 0.87 -2.3 1,566,176 + Synpcc7942_1515 0.87 -2.7 1,566,233 + -2.1 1,566,240 - -3.2 1,566,240 - -1.3 1,566,240 - -0.5 1,566,241 - -2.1 1,566,272 + +0.1 1,566,284 + +2.3 1,566,313 + -2.1 1,566,353 + -3.6 1,566,355 - +0.1 1,566,490 + Synpcc7942_1516 0.26 -1.7 1,566,559 + Synpcc7942_1516 0.36 -0.5 1,566,981 + -2.8 1,566,989 + +0.5 1,566,989 + -0.5 1,566,989 + -3.7 1,566,989 + -1.9 1,566,997 - -2.8 1,567,675 - Synpcc7942_1517 0.53 -1.1
Or see this region's nucleotide sequence