Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murE and Synpcc7942_1485 are separated by 39 nucleotides Synpcc7942_1485 and Synpcc7942_1486 overlap by 10 nucleotides Synpcc7942_1486 and rpsD are separated by 73 nucleotides
Synpcc7942_1484: murE - UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase, at 1,534,874 to 1,536,367
murE
Synpcc7942_1485: Synpcc7942_1485 - hypothetical protein, at 1,536,407 to 1,536,673
_1485
Synpcc7942_1486: Synpcc7942_1486 - Protein of unknown function DUF37, at 1,536,664 to 1,536,912
_1486
Synpcc7942_1487: rpsD - 30S ribosomal protein S4, at 1,536,986 to 1,537,594
rpsD
Position (kb)
1536
1537 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 1536.382 kb on - strand at 1536.387 kb on - strand at 1536.405 kb on + strand at 1536.405 kb on + strand at 1536.413 kb on - strand at 1536.448 kb on + strand, within Synpcc7942_1485 at 1536.474 kb on - strand, within Synpcc7942_1485 at 1536.556 kb on - strand, within Synpcc7942_1485 at 1536.561 kb on - strand, within Synpcc7942_1485 at 1536.623 kb on - strand, within Synpcc7942_1485 at 1536.625 kb on + strand, within Synpcc7942_1485 at 1536.638 kb on - strand, within Synpcc7942_1485 at 1536.656 kb on + strand at 1536.659 kb on - strand at 1536.664 kb on - strand at 1536.670 kb on - strand at 1536.689 kb on + strand, within Synpcc7942_1486 at 1536.714 kb on + strand, within Synpcc7942_1486 at 1536.737 kb on + strand, within Synpcc7942_1486 at 1536.753 kb on - strand at 1536.770 kb on - strand, within Synpcc7942_1486 at 1536.783 kb on - strand, within Synpcc7942_1486 at 1536.801 kb on - strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.804 kb on + strand, within Synpcc7942_1486 at 1536.812 kb on - strand, within Synpcc7942_1486 at 1536.812 kb on - strand, within Synpcc7942_1486 at 1536.812 kb on - strand, within Synpcc7942_1486 at 1536.812 kb on - strand, within Synpcc7942_1486 at 1536.816 kb on + strand, within Synpcc7942_1486 at 1536.826 kb on + strand, within Synpcc7942_1486 at 1536.826 kb on + strand, within Synpcc7942_1486 at 1536.838 kb on - strand, within Synpcc7942_1486 at 1536.891 kb on + strand at 1537.615 kb on - strand at 1537.615 kb on - strand at 1537.637 kb on + strand at 1537.646 kb on - strand at 1537.648 kb on + strand at 1537.678 kb on - strand at 1537.680 kb on + strand at 1537.687 kb on + strand at 1537.687 kb on + strand at 1537.688 kb on - strand at 1537.690 kb on - strand at 1537.693 kb on - strand at 1537.695 kb on - strand at 1537.697 kb on + strand at 1537.704 kb on + strand at 1537.761 kb on - strand at 1537.764 kb on + strand at 1537.774 kb on + strand at 1537.784 kb on - strand at 1537.811 kb on + strand at 1537.819 kb on - strand at 1537.824 kb on - strand at 1537.850 kb on - strand at 1537.850 kb on - strand at 1537.857 kb on - strand at 1537.870 kb on + strand at 1537.906 kb on + strand at 1537.906 kb on + strand at 1537.906 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,536,382 - -1.4 1,536,387 - -2.8 1,536,405 + -3.6 1,536,405 + -0.9 1,536,413 - -1.2 1,536,448 + Synpcc7942_1485 0.15 +0.9 1,536,474 - Synpcc7942_1485 0.25 +6.8 1,536,556 - Synpcc7942_1485 0.56 -2.7 1,536,561 - Synpcc7942_1485 0.58 +1.1 1,536,623 - Synpcc7942_1485 0.81 -1.8 1,536,625 + Synpcc7942_1485 0.82 -1.2 1,536,638 - Synpcc7942_1485 0.87 -3.0 1,536,656 + -2.9 1,536,659 - -3.4 1,536,664 - -2.0 1,536,670 - -2.3 1,536,689 + Synpcc7942_1486 0.10 -2.6 1,536,714 + Synpcc7942_1486 0.20 -2.0 1,536,737 + Synpcc7942_1486 0.29 -2.6 1,536,753 - -0.1 1,536,770 - Synpcc7942_1486 0.43 -1.2 1,536,783 - Synpcc7942_1486 0.48 -2.3 1,536,801 - Synpcc7942_1486 0.55 -4.1 1,536,804 + Synpcc7942_1486 0.56 -0.2 1,536,804 + Synpcc7942_1486 0.56 -0.5 1,536,804 + Synpcc7942_1486 0.56 -2.2 1,536,804 + Synpcc7942_1486 0.56 -2.1 1,536,804 + Synpcc7942_1486 0.56 -2.0 1,536,804 + Synpcc7942_1486 0.56 -0.1 1,536,804 + Synpcc7942_1486 0.56 -1.5 1,536,804 + Synpcc7942_1486 0.56 -2.1 1,536,812 - Synpcc7942_1486 0.59 -2.4 1,536,812 - Synpcc7942_1486 0.59 -1.7 1,536,812 - Synpcc7942_1486 0.59 -2.3 1,536,812 - Synpcc7942_1486 0.59 -2.4 1,536,816 + Synpcc7942_1486 0.61 -0.4 1,536,826 + Synpcc7942_1486 0.65 -2.4 1,536,826 + Synpcc7942_1486 0.65 -2.6 1,536,838 - Synpcc7942_1486 0.70 -1.9 1,536,891 + -4.2 1,537,615 - +1.3 1,537,615 - -2.2 1,537,637 + +2.1 1,537,646 - +1.8 1,537,648 + -0.0 1,537,678 - -2.9 1,537,680 + -1.1 1,537,687 + -1.7 1,537,687 + +1.0 1,537,688 - +1.3 1,537,690 - +2.6 1,537,693 - -0.6 1,537,695 - +2.3 1,537,697 + +0.5 1,537,704 + +0.5 1,537,761 - +3.8 1,537,764 + +2.2 1,537,774 + -1.8 1,537,784 - -2.6 1,537,811 + -0.9 1,537,819 - -2.2 1,537,824 - -1.9 1,537,850 - -2.4 1,537,850 - -2.9 1,537,857 - -1.9 1,537,870 + +0.6 1,537,906 + -2.1 1,537,906 + -0.2 1,537,906 + +0.7
Or see this region's nucleotide sequence