Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1480 and hisH overlap by 4 nucleotides hisH and Synpcc7942_1482 are separated by 206 nucleotides Synpcc7942_1482 and Synpcc7942_1483 are separated by 278 nucleotides Synpcc7942_1483 and murE overlap by 4 nucleotides
Synpcc7942_1480: Synpcc7942_1480 - hypothetical protein, at 1,531,194 to 1,531,748
_1480
Synpcc7942_1481: hisH - imidazole glycerol phosphate synthase subunit HisH, at 1,531,745 to 1,532,398
hisH
Synpcc7942_1482: Synpcc7942_1482 - hypothetical protein, at 1,532,605 to 1,534,170
_1482
Synpcc7942_1483: Synpcc7942_1483 - hypothetical protein, at 1,534,449 to 1,534,877
_1483
Synpcc7942_1484: murE - UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase, at 1,534,874 to 1,536,367
murE
Position (kb)
1532
1533
1534
1535 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 1531.659 kb on - strand, within Synpcc7942_1480 at 1531.659 kb on - strand, within Synpcc7942_1480 at 1531.695 kb on + strand at 1531.755 kb on + strand at 1531.763 kb on - strand at 1532.408 kb on - strand at 1532.545 kb on + strand at 1532.553 kb on - strand at 1532.553 kb on - strand at 1532.553 kb on - strand at 1532.554 kb on - strand at 1532.556 kb on + strand at 1532.556 kb on + strand at 1532.556 kb on + strand at 1532.564 kb on - strand at 1532.570 kb on + strand at 1532.570 kb on + strand at 1532.578 kb on - strand at 1532.578 kb on - strand at 1532.624 kb on - strand at 1532.747 kb on + strand at 1532.800 kb on + strand, within Synpcc7942_1482 at 1532.950 kb on + strand, within Synpcc7942_1482 at 1532.984 kb on - strand, within Synpcc7942_1482 at 1532.985 kb on - strand, within Synpcc7942_1482 at 1533.035 kb on + strand, within Synpcc7942_1482 at 1533.083 kb on - strand, within Synpcc7942_1482 at 1533.207 kb on + strand, within Synpcc7942_1482 at 1533.244 kb on + strand, within Synpcc7942_1482 at 1533.391 kb on - strand, within Synpcc7942_1482 at 1533.411 kb on - strand, within Synpcc7942_1482 at 1533.438 kb on - strand, within Synpcc7942_1482 at 1533.477 kb on - strand, within Synpcc7942_1482 at 1533.581 kb on - strand, within Synpcc7942_1482 at 1533.786 kb on - strand, within Synpcc7942_1482 at 1533.803 kb on - strand, within Synpcc7942_1482 at 1533.854 kb on + strand, within Synpcc7942_1482 at 1534.003 kb on - strand, within Synpcc7942_1482 at 1534.053 kb on - strand at 1534.067 kb on - strand at 1534.099 kb on + strand at 1534.106 kb on + strand at 1534.114 kb on - strand at 1534.114 kb on - strand at 1534.114 kb on - strand at 1534.116 kb on + strand at 1534.124 kb on - strand at 1534.172 kb on - strand at 1534.215 kb on - strand at 1534.231 kb on + strand at 1534.263 kb on - strand at 1534.292 kb on - strand at 1534.315 kb on - strand at 1534.371 kb on + strand at 1534.372 kb on + strand at 1534.389 kb on + strand at 1534.391 kb on + strand at 1534.391 kb on + strand at 1534.391 kb on + strand at 1534.391 kb on + strand at 1534.397 kb on - strand at 1534.397 kb on - strand at 1534.404 kb on + strand at 1534.412 kb on - strand at 1534.423 kb on + strand at 1534.433 kb on - strand at 1534.441 kb on + strand at 1534.480 kb on - strand at 1534.480 kb on - strand at 1534.493 kb on + strand, within Synpcc7942_1483 at 1534.522 kb on - strand, within Synpcc7942_1483 at 1534.527 kb on - strand, within Synpcc7942_1483 at 1534.545 kb on - strand, within Synpcc7942_1483 at 1534.555 kb on - strand, within Synpcc7942_1483 at 1534.566 kb on + strand, within Synpcc7942_1483 at 1534.584 kb on + strand, within Synpcc7942_1483 at 1534.584 kb on + strand, within Synpcc7942_1483 at 1534.584 kb on + strand, within Synpcc7942_1483 at 1534.637 kb on + strand, within Synpcc7942_1483 at 1534.644 kb on - strand, within Synpcc7942_1483 at 1534.730 kb on - strand, within Synpcc7942_1483 at 1534.730 kb on - strand, within Synpcc7942_1483 at 1534.736 kb on - strand, within Synpcc7942_1483 at 1534.736 kb on - strand, within Synpcc7942_1483 at 1534.760 kb on + strand, within Synpcc7942_1483 at 1534.774 kb on - strand, within Synpcc7942_1483 at 1534.827 kb on - strand, within Synpcc7942_1483 at 1534.832 kb on + strand, within Synpcc7942_1483 at 1534.832 kb on + strand, within Synpcc7942_1483 at 1534.832 kb on + strand, within Synpcc7942_1483 at 1534.832 kb on + strand, within Synpcc7942_1483 at 1534.832 kb on + strand, within Synpcc7942_1483 at 1534.832 kb on + strand, within Synpcc7942_1483 at 1534.832 kb on + strand, within Synpcc7942_1483 at 1534.833 kb on + strand, within Synpcc7942_1483 at 1534.840 kb on - strand at 1534.840 kb on - strand at 1534.840 kb on - strand at 1534.840 kb on - strand at 1534.859 kb on - strand at 1534.875 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,531,659 - Synpcc7942_1480 0.84 -0.7 1,531,659 - Synpcc7942_1480 0.84 +0.5 1,531,695 + +0.9 1,531,755 + -0.1 1,531,763 - +1.1 1,532,408 - -1.8 1,532,545 + +0.2 1,532,553 - -3.9 1,532,553 - -1.9 1,532,553 - +1.1 1,532,554 - -2.2 1,532,556 + -3.5 1,532,556 + -2.0 1,532,556 + -1.9 1,532,564 - -2.5 1,532,570 + -3.6 1,532,570 + -2.5 1,532,578 - -2.6 1,532,578 - -1.6 1,532,624 - -4.7 1,532,747 + -3.3 1,532,800 + Synpcc7942_1482 0.12 -3.9 1,532,950 + Synpcc7942_1482 0.22 -0.8 1,532,984 - Synpcc7942_1482 0.24 -3.0 1,532,985 - Synpcc7942_1482 0.24 -2.8 1,533,035 + Synpcc7942_1482 0.27 -1.9 1,533,083 - Synpcc7942_1482 0.31 -5.8 1,533,207 + Synpcc7942_1482 0.38 -5.1 1,533,244 + Synpcc7942_1482 0.41 -0.1 1,533,391 - Synpcc7942_1482 0.50 -2.4 1,533,411 - Synpcc7942_1482 0.51 -2.8 1,533,438 - Synpcc7942_1482 0.53 -3.4 1,533,477 - Synpcc7942_1482 0.56 -3.5 1,533,581 - Synpcc7942_1482 0.62 -3.4 1,533,786 - Synpcc7942_1482 0.75 -2.2 1,533,803 - Synpcc7942_1482 0.77 -2.0 1,533,854 + Synpcc7942_1482 0.80 -2.8 1,534,003 - Synpcc7942_1482 0.89 -2.8 1,534,053 - -2.2 1,534,067 - -4.1 1,534,099 + -3.7 1,534,106 + -5.2 1,534,114 - -2.4 1,534,114 - -1.2 1,534,114 - -3.4 1,534,116 + -2.5 1,534,124 - -3.4 1,534,172 - +7.3 1,534,215 - -1.1 1,534,231 + +0.5 1,534,263 - -1.6 1,534,292 - -0.4 1,534,315 - -0.0 1,534,371 + +0.4 1,534,372 + -3.4 1,534,389 + -1.1 1,534,391 + +0.7 1,534,391 + -1.7 1,534,391 + +0.8 1,534,391 + -0.0 1,534,397 - -3.4 1,534,397 - +1.8 1,534,404 + -1.7 1,534,412 - -1.3 1,534,423 + +2.3 1,534,433 - +2.6 1,534,441 + -0.5 1,534,480 - +0.0 1,534,480 - -0.1 1,534,493 + Synpcc7942_1483 0.10 +0.9 1,534,522 - Synpcc7942_1483 0.17 +0.6 1,534,527 - Synpcc7942_1483 0.18 -1.2 1,534,545 - Synpcc7942_1483 0.22 -1.6 1,534,555 - Synpcc7942_1483 0.25 +1.7 1,534,566 + Synpcc7942_1483 0.27 +3.2 1,534,584 + Synpcc7942_1483 0.31 -0.7 1,534,584 + Synpcc7942_1483 0.31 +1.5 1,534,584 + Synpcc7942_1483 0.31 +1.6 1,534,637 + Synpcc7942_1483 0.44 -0.2 1,534,644 - Synpcc7942_1483 0.45 -2.4 1,534,730 - Synpcc7942_1483 0.66 -1.6 1,534,730 - Synpcc7942_1483 0.66 -1.4 1,534,736 - Synpcc7942_1483 0.67 -2.9 1,534,736 - Synpcc7942_1483 0.67 +1.3 1,534,760 + Synpcc7942_1483 0.72 -0.9 1,534,774 - Synpcc7942_1483 0.76 +2.5 1,534,827 - Synpcc7942_1483 0.88 -0.9 1,534,832 + Synpcc7942_1483 0.89 -2.7 1,534,832 + Synpcc7942_1483 0.89 +2.3 1,534,832 + Synpcc7942_1483 0.89 +0.6 1,534,832 + Synpcc7942_1483 0.89 +1.6 1,534,832 + Synpcc7942_1483 0.89 +1.4 1,534,832 + Synpcc7942_1483 0.89 -0.2 1,534,832 + Synpcc7942_1483 0.89 +1.4 1,534,833 + Synpcc7942_1483 0.90 -1.2 1,534,840 - +0.4 1,534,840 - +3.7 1,534,840 - -1.1 1,534,840 - +1.0 1,534,859 - +1.3 1,534,875 - -1.4
Or see this region's nucleotide sequence