Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ndhD5 and Synpcc7942_1474 overlap by 4 nucleotides Synpcc7942_1474 and sbtA are separated by 419 nucleotides sbtA and Synpcc7942_1476 are separated by 762 nucleotides
Synpcc7942_1473: ndhD5 - putative monovalent cation/H+ antiporter subunit D, at 1,523,639 to 1,525,081
ndhD5
Synpcc7942_1474: Synpcc7942_1474 - putative monovalent cation/H+ antiporter subunit C, at 1,525,078 to 1,525,413
_1474
Synpcc7942_1475: sbtA - sodium-dependent bicarbonate transporter, at 1,525,833 to 1,526,954
sbtA
Synpcc7942_1476: Synpcc7942_1476 - hypothetical protein, at 1,527,717 to 1,528,031
_1476
Position (kb)
1525
1526
1527 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1524.843 kb on - strand, within ndhD5 at 1524.860 kb on - strand, within ndhD5 at 1524.903 kb on - strand, within ndhD5 at 1524.977 kb on - strand at 1524.999 kb on - strand at 1525.083 kb on - strand at 1525.159 kb on - strand, within Synpcc7942_1474 at 1525.273 kb on - strand, within Synpcc7942_1474 at 1525.396 kb on + strand at 1525.767 kb on + strand at 1525.767 kb on + strand at 1525.804 kb on + strand at 1525.812 kb on - strand at 1525.934 kb on - strand at 1525.935 kb on - strand at 1526.020 kb on - strand, within sbtA at 1526.052 kb on + strand, within sbtA at 1526.114 kb on - strand, within sbtA at 1526.118 kb on + strand, within sbtA at 1526.128 kb on + strand, within sbtA at 1526.128 kb on + strand, within sbtA at 1526.128 kb on + strand, within sbtA at 1526.136 kb on - strand, within sbtA at 1526.293 kb on - strand, within sbtA at 1526.293 kb on - strand, within sbtA at 1526.338 kb on + strand, within sbtA at 1526.344 kb on + strand, within sbtA at 1526.346 kb on + strand, within sbtA at 1526.390 kb on + strand, within sbtA at 1526.425 kb on + strand, within sbtA at 1526.479 kb on + strand, within sbtA at 1526.584 kb on - strand, within sbtA at 1526.594 kb on - strand, within sbtA at 1526.594 kb on - strand, within sbtA at 1526.628 kb on - strand, within sbtA at 1526.656 kb on + strand, within sbtA at 1526.664 kb on - strand, within sbtA at 1526.737 kb on + strand, within sbtA at 1527.134 kb on + strand at 1527.383 kb on - strand at 1527.383 kb on - strand at 1527.395 kb on + strand at 1527.428 kb on - strand at 1527.438 kb on - strand at 1527.438 kb on - strand at 1527.438 kb on - strand at 1527.438 kb on - strand at 1527.463 kb on + strand at 1527.528 kb on - strand at 1527.534 kb on + strand at 1527.592 kb on + strand at 1527.610 kb on + strand at 1527.626 kb on + strand at 1527.746 kb on - strand at 1527.806 kb on + strand, within Synpcc7942_1476 at 1527.807 kb on + strand, within Synpcc7942_1476 at 1527.814 kb on - strand, within Synpcc7942_1476 at 1527.814 kb on - strand, within Synpcc7942_1476 at 1527.826 kb on - strand, within Synpcc7942_1476 at 1527.849 kb on - strand, within Synpcc7942_1476 at 1527.856 kb on + strand, within Synpcc7942_1476 at 1527.856 kb on + strand, within Synpcc7942_1476 at 1527.881 kb on + strand, within Synpcc7942_1476
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,524,843 - ndhD5 Synpcc7942_1473 0.83 -0.9 1,524,860 - ndhD5 Synpcc7942_1473 0.85 -1.3 1,524,903 - ndhD5 Synpcc7942_1473 0.88 +0.1 1,524,977 - -1.4 1,524,999 - -2.9 1,525,083 - -1.5 1,525,159 - Synpcc7942_1474 0.24 -1.4 1,525,273 - Synpcc7942_1474 0.58 +2.1 1,525,396 + +0.5 1,525,767 + +0.5 1,525,767 + +0.5 1,525,804 + -2.7 1,525,812 - -1.9 1,525,934 - -3.6 1,525,935 - +0.6 1,526,020 - sbtA Synpcc7942_1475 0.17 -4.2 1,526,052 + sbtA Synpcc7942_1475 0.20 -2.8 1,526,114 - sbtA Synpcc7942_1475 0.25 -3.2 1,526,118 + sbtA Synpcc7942_1475 0.25 -1.5 1,526,128 + sbtA Synpcc7942_1475 0.26 -1.3 1,526,128 + sbtA Synpcc7942_1475 0.26 -2.3 1,526,128 + sbtA Synpcc7942_1475 0.26 -3.3 1,526,136 - sbtA Synpcc7942_1475 0.27 -1.3 1,526,293 - sbtA Synpcc7942_1475 0.41 -2.6 1,526,293 - sbtA Synpcc7942_1475 0.41 -2.3 1,526,338 + sbtA Synpcc7942_1475 0.45 -2.8 1,526,344 + sbtA Synpcc7942_1475 0.46 -1.8 1,526,346 + sbtA Synpcc7942_1475 0.46 -2.6 1,526,390 + sbtA Synpcc7942_1475 0.50 -2.5 1,526,425 + sbtA Synpcc7942_1475 0.53 -3.0 1,526,479 + sbtA Synpcc7942_1475 0.58 -3.2 1,526,584 - sbtA Synpcc7942_1475 0.67 -2.1 1,526,594 - sbtA Synpcc7942_1475 0.68 -3.7 1,526,594 - sbtA Synpcc7942_1475 0.68 -1.6 1,526,628 - sbtA Synpcc7942_1475 0.71 -2.7 1,526,656 + sbtA Synpcc7942_1475 0.73 -1.9 1,526,664 - sbtA Synpcc7942_1475 0.74 -2.3 1,526,737 + sbtA Synpcc7942_1475 0.81 -1.6 1,527,134 + -1.4 1,527,383 - +3.0 1,527,383 - +3.2 1,527,395 + -2.4 1,527,428 - -2.2 1,527,438 - +2.6 1,527,438 - +0.7 1,527,438 - -1.3 1,527,438 - -1.0 1,527,463 + +1.0 1,527,528 - -1.6 1,527,534 + -2.4 1,527,592 + -1.8 1,527,610 + +0.7 1,527,626 + +0.6 1,527,746 - +1.5 1,527,806 + Synpcc7942_1476 0.28 -1.0 1,527,807 + Synpcc7942_1476 0.29 -0.8 1,527,814 - Synpcc7942_1476 0.31 -0.0 1,527,814 - Synpcc7942_1476 0.31 -2.7 1,527,826 - Synpcc7942_1476 0.35 -1.3 1,527,849 - Synpcc7942_1476 0.42 -1.1 1,527,856 + Synpcc7942_1476 0.44 -0.8 1,527,856 + Synpcc7942_1476 0.44 -1.2 1,527,881 + Synpcc7942_1476 0.52 +0.7
Or see this region's nucleotide sequence