Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1469 and Synpcc7942_1470 overlap by 8 nucleotides Synpcc7942_1470 and Synpcc7942_1471 overlap by 4 nucleotides Synpcc7942_1471 and Synpcc7942_1472 overlap by 4 nucleotides Synpcc7942_1472 and ndhD5 overlap by 4 nucleotides ndhD5 and Synpcc7942_1474 overlap by 4 nucleotides Synpcc7942_1474 and sbtA are separated by 419 nucleotides
Synpcc7942_1469: Synpcc7942_1469 - hypothetical protein, at 1,522,138 to 1,522,719
_1469
Synpcc7942_1470: Synpcc7942_1470 - hypothetical protein, at 1,522,712 to 1,523,005
_1470
Synpcc7942_1471: Synpcc7942_1471 - hypothetical protein, at 1,523,002 to 1,523,259
_1471
Synpcc7942_1472: Synpcc7942_1472 - hypothetical protein, at 1,523,256 to 1,523,642
_1472
Synpcc7942_1473: ndhD5 - putative monovalent cation/H+ antiporter subunit D, at 1,523,639 to 1,525,081
ndhD5
Synpcc7942_1474: Synpcc7942_1474 - putative monovalent cation/H+ antiporter subunit C, at 1,525,078 to 1,525,413
_1474
Synpcc7942_1475: sbtA - sodium-dependent bicarbonate transporter, at 1,525,833 to 1,526,954
sbtA
Position (kb)
1523
1524
1525
1526 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1522.720 kb on + strand at 1522.784 kb on + strand, within Synpcc7942_1470 at 1522.944 kb on - strand, within Synpcc7942_1470 at 1523.284 kb on + strand at 1523.330 kb on - strand, within Synpcc7942_1472 at 1523.378 kb on + strand, within Synpcc7942_1472 at 1523.854 kb on + strand, within ndhD5 at 1524.088 kb on - strand, within ndhD5 at 1524.214 kb on + strand, within ndhD5 at 1524.222 kb on - strand, within ndhD5 at 1524.315 kb on + strand, within ndhD5 at 1524.843 kb on - strand, within ndhD5 at 1524.860 kb on - strand, within ndhD5 at 1524.903 kb on - strand, within ndhD5 at 1524.977 kb on - strand at 1524.999 kb on - strand at 1525.083 kb on - strand at 1525.159 kb on - strand, within Synpcc7942_1474 at 1525.273 kb on - strand, within Synpcc7942_1474 at 1525.396 kb on + strand at 1525.767 kb on + strand at 1525.767 kb on + strand at 1525.804 kb on + strand at 1525.812 kb on - strand at 1525.934 kb on - strand at 1525.935 kb on - strand at 1526.020 kb on - strand, within sbtA at 1526.052 kb on + strand, within sbtA
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,522,720 + -1.5 1,522,784 + Synpcc7942_1470 0.24 -1.0 1,522,944 - Synpcc7942_1470 0.79 -2.5 1,523,284 + -1.6 1,523,330 - Synpcc7942_1472 0.19 -2.8 1,523,378 + Synpcc7942_1472 0.32 -0.9 1,523,854 + ndhD5 Synpcc7942_1473 0.15 -2.4 1,524,088 - ndhD5 Synpcc7942_1473 0.31 -1.4 1,524,214 + ndhD5 Synpcc7942_1473 0.40 -2.8 1,524,222 - ndhD5 Synpcc7942_1473 0.40 -1.4 1,524,315 + ndhD5 Synpcc7942_1473 0.47 -0.9 1,524,843 - ndhD5 Synpcc7942_1473 0.83 -0.9 1,524,860 - ndhD5 Synpcc7942_1473 0.85 -1.3 1,524,903 - ndhD5 Synpcc7942_1473 0.88 +0.1 1,524,977 - -1.4 1,524,999 - -2.9 1,525,083 - -1.5 1,525,159 - Synpcc7942_1474 0.24 -1.4 1,525,273 - Synpcc7942_1474 0.58 +2.1 1,525,396 + +0.5 1,525,767 + +0.5 1,525,767 + +0.5 1,525,804 + -2.7 1,525,812 - -1.9 1,525,934 - -3.6 1,525,935 - +0.6 1,526,020 - sbtA Synpcc7942_1475 0.17 -4.2 1,526,052 + sbtA Synpcc7942_1475 0.20 -2.8
Or see this region's nucleotide sequence