Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysM and Synpcc7942_1467 are separated by 5 nucleotides Synpcc7942_1467 and Synpcc7942_1468 are separated by 118 nucleotides Synpcc7942_1468 and Synpcc7942_1469 overlap by 4 nucleotides Synpcc7942_1469 and Synpcc7942_1470 overlap by 8 nucleotides Synpcc7942_1470 and Synpcc7942_1471 overlap by 4 nucleotides
Synpcc7942_1466: cysM - cysteine synthase, at 1,519,664 to 1,520,668
cysM
Synpcc7942_1467: Synpcc7942_1467 - Heat shock protein DnaJ-like, at 1,520,674 to 1,521,363
_1467
Synpcc7942_1468: Synpcc7942_1468 - putative monovalent cation/H+ antiporter subunit B, at 1,521,482 to 1,522,141
_1468
Synpcc7942_1469: Synpcc7942_1469 - hypothetical protein, at 1,522,138 to 1,522,719
_1469
Synpcc7942_1470: Synpcc7942_1470 - hypothetical protein, at 1,522,712 to 1,523,005
_1470
Synpcc7942_1471: Synpcc7942_1471 - hypothetical protein, at 1,523,002 to 1,523,259
_1471
Position (kb)
1521
1522
1523 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1520.500 kb on - strand, within cysM at 1520.595 kb on + strand at 1520.595 kb on + strand at 1520.595 kb on + strand at 1520.608 kb on - strand at 1520.621 kb on + strand at 1520.691 kb on + strand at 1520.763 kb on - strand, within Synpcc7942_1467 at 1520.827 kb on + strand, within Synpcc7942_1467 at 1520.858 kb on + strand, within Synpcc7942_1467 at 1520.858 kb on + strand, within Synpcc7942_1467 at 1520.863 kb on + strand, within Synpcc7942_1467 at 1520.895 kb on + strand, within Synpcc7942_1467 at 1520.897 kb on + strand, within Synpcc7942_1467 at 1520.911 kb on + strand, within Synpcc7942_1467 at 1520.971 kb on - strand, within Synpcc7942_1467 at 1520.971 kb on - strand, within Synpcc7942_1467 at 1520.993 kb on - strand, within Synpcc7942_1467 at 1521.006 kb on - strand, within Synpcc7942_1467 at 1521.038 kb on - strand, within Synpcc7942_1467 at 1521.048 kb on - strand, within Synpcc7942_1467 at 1521.066 kb on - strand, within Synpcc7942_1467 at 1521.069 kb on - strand, within Synpcc7942_1467 at 1521.096 kb on + strand, within Synpcc7942_1467 at 1521.166 kb on + strand, within Synpcc7942_1467 at 1521.213 kb on + strand, within Synpcc7942_1467 at 1521.374 kb on - strand at 1521.376 kb on + strand at 1521.399 kb on + strand at 1521.480 kb on + strand at 1521.584 kb on + strand, within Synpcc7942_1468 at 1521.599 kb on + strand, within Synpcc7942_1468 at 1521.614 kb on - strand, within Synpcc7942_1468 at 1521.795 kb on - strand, within Synpcc7942_1468 at 1522.284 kb on + strand, within Synpcc7942_1469 at 1522.332 kb on - strand, within Synpcc7942_1469 at 1522.332 kb on - strand, within Synpcc7942_1469 at 1522.486 kb on + strand, within Synpcc7942_1469 at 1522.530 kb on - strand, within Synpcc7942_1469 at 1522.541 kb on - strand, within Synpcc7942_1469 at 1522.580 kb on + strand, within Synpcc7942_1469 at 1522.587 kb on - strand, within Synpcc7942_1469 at 1522.587 kb on - strand, within Synpcc7942_1469 at 1522.587 kb on - strand, within Synpcc7942_1469 at 1522.587 kb on - strand, within Synpcc7942_1469 at 1522.587 kb on - strand, within Synpcc7942_1469 at 1522.589 kb on + strand, within Synpcc7942_1469 at 1522.597 kb on - strand, within Synpcc7942_1469 at 1522.597 kb on - strand, within Synpcc7942_1469 at 1522.720 kb on + strand at 1522.784 kb on + strand, within Synpcc7942_1470 at 1522.944 kb on - strand, within Synpcc7942_1470
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,520,500 - cysM Synpcc7942_1466 0.83 +1.9 1,520,595 + +0.1 1,520,595 + -0.9 1,520,595 + -2.2 1,520,608 - +0.7 1,520,621 + +0.7 1,520,691 + +0.0 1,520,763 - Synpcc7942_1467 0.13 -1.3 1,520,827 + Synpcc7942_1467 0.22 +1.7 1,520,858 + Synpcc7942_1467 0.27 -0.2 1,520,858 + Synpcc7942_1467 0.27 +2.2 1,520,863 + Synpcc7942_1467 0.27 -2.0 1,520,895 + Synpcc7942_1467 0.32 +2.0 1,520,897 + Synpcc7942_1467 0.32 -1.5 1,520,911 + Synpcc7942_1467 0.34 -2.3 1,520,971 - Synpcc7942_1467 0.43 -1.5 1,520,971 - Synpcc7942_1467 0.43 +0.5 1,520,993 - Synpcc7942_1467 0.46 -0.2 1,521,006 - Synpcc7942_1467 0.48 -1.6 1,521,038 - Synpcc7942_1467 0.53 +0.7 1,521,048 - Synpcc7942_1467 0.54 -0.3 1,521,066 - Synpcc7942_1467 0.57 +0.3 1,521,069 - Synpcc7942_1467 0.57 +0.7 1,521,096 + Synpcc7942_1467 0.61 -1.0 1,521,166 + Synpcc7942_1467 0.71 -0.0 1,521,213 + Synpcc7942_1467 0.78 +2.8 1,521,374 - +2.1 1,521,376 + -1.4 1,521,399 + +0.4 1,521,480 + +2.9 1,521,584 + Synpcc7942_1468 0.15 -1.0 1,521,599 + Synpcc7942_1468 0.18 -1.0 1,521,614 - Synpcc7942_1468 0.20 -2.0 1,521,795 - Synpcc7942_1468 0.47 +0.5 1,522,284 + Synpcc7942_1469 0.25 -0.3 1,522,332 - Synpcc7942_1469 0.33 +0.5 1,522,332 - Synpcc7942_1469 0.33 -0.8 1,522,486 + Synpcc7942_1469 0.60 -0.3 1,522,530 - Synpcc7942_1469 0.67 +0.5 1,522,541 - Synpcc7942_1469 0.69 -1.0 1,522,580 + Synpcc7942_1469 0.76 -0.3 1,522,587 - Synpcc7942_1469 0.77 +1.0 1,522,587 - Synpcc7942_1469 0.77 -0.9 1,522,587 - Synpcc7942_1469 0.77 -1.7 1,522,587 - Synpcc7942_1469 0.77 -1.1 1,522,587 - Synpcc7942_1469 0.77 +1.7 1,522,589 + Synpcc7942_1469 0.77 -0.5 1,522,597 - Synpcc7942_1469 0.79 -0.8 1,522,597 - Synpcc7942_1469 0.79 -3.5 1,522,720 + -1.5 1,522,784 + Synpcc7942_1470 0.24 -1.0 1,522,944 - Synpcc7942_1470 0.79 -2.5
Or see this region's nucleotide sequence