Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt somB and somA are separated by 236 nucleotides somA and Synpcc7942_1465 are separated by 161 nucleotides Synpcc7942_1465 and cysM are separated by 214 nucleotides
Synpcc7942_1463: somB - probable porin, at 1,515,369 to 1,517,000
somB
Synpcc7942_1464: somA - probable porin, at 1,517,237 to 1,518,832
somA
Synpcc7942_1465: Synpcc7942_1465 - transcriptional regulator, BadM/Rrf2 family, at 1,518,994 to 1,519,449
_1465
Synpcc7942_1466: cysM - cysteine synthase, at 1,519,664 to 1,520,668
cysM
Position (kb) 1517
1518
1519 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 1516.268 kb on + strand, within somB at 1516.271 kb on + strand, within somB at 1516.329 kb on + strand, within somB at 1516.337 kb on - strand, within somB at 1516.337 kb on - strand, within somB at 1516.367 kb on - strand, within somB at 1516.390 kb on - strand, within somB at 1516.394 kb on + strand, within somB at 1516.402 kb on - strand, within somB at 1516.406 kb on + strand at 1516.406 kb on + strand, within somB at 1516.407 kb on - strand, within somB at 1516.422 kb on - strand, within somB at 1516.444 kb on + strand, within somB at 1516.444 kb on + strand, within somB at 1516.452 kb on - strand, within somB at 1516.452 kb on - strand, within somB at 1516.461 kb on + strand, within somB at 1516.466 kb on + strand, within somB at 1516.466 kb on + strand, within somB at 1516.466 kb on + strand, within somB at 1516.466 kb on + strand, within somB at 1516.466 kb on + strand, within somB at 1516.466 kb on + strand, within somB at 1516.474 kb on - strand, within somB at 1516.474 kb on - strand, within somB at 1516.474 kb on - strand, within somB at 1516.474 kb on - strand, within somB at 1516.474 kb on - strand, within somB at 1516.474 kb on - strand, within somB at 1516.474 kb on - strand, within somB at 1516.474 kb on - strand, within somB at 1516.474 kb on - strand, within somB at 1516.486 kb on + strand, within somB at 1516.516 kb on + strand, within somB at 1516.567 kb on + strand, within somB at 1516.572 kb on + strand, within somB at 1516.572 kb on + strand, within somB at 1516.572 kb on + strand, within somB at 1516.577 kb on + strand, within somB at 1516.580 kb on - strand, within somB at 1516.580 kb on - strand, within somB at 1516.601 kb on - strand, within somB at 1516.610 kb on + strand, within somB at 1516.622 kb on + strand, within somB at 1516.628 kb on + strand, within somB at 1516.628 kb on + strand, within somB at 1516.638 kb on - strand, within somB at 1516.648 kb on - strand, within somB at 1516.672 kb on + strand, within somB at 1516.690 kb on + strand, within somB at 1516.710 kb on + strand, within somB at 1516.710 kb on + strand, within somB at 1516.715 kb on + strand, within somB at 1516.715 kb on + strand, within somB at 1516.715 kb on + strand, within somB at 1516.723 kb on - strand, within somB at 1516.737 kb on + strand, within somB at 1516.745 kb on + strand, within somB at 1516.771 kb on + strand, within somB at 1516.779 kb on - strand, within somB at 1516.781 kb on + strand, within somB at 1516.781 kb on + strand, within somB at 1516.783 kb on + strand, within somB at 1516.791 kb on + strand, within somB at 1516.804 kb on + strand, within somB at 1516.821 kb on + strand, within somB at 1516.824 kb on + strand, within somB at 1516.824 kb on + strand, within somB at 1516.894 kb on + strand at 1516.894 kb on + strand at 1516.910 kb on - strand at 1516.929 kb on + strand at 1516.937 kb on - strand at 1516.937 kb on - strand at 1517.003 kb on - strand at 1517.025 kb on - strand at 1517.042 kb on - strand at 1517.050 kb on + strand at 1517.058 kb on - strand at 1517.058 kb on - strand at 1517.094 kb on - strand at 1517.096 kb on + strand at 1517.312 kb on + strand at 1517.327 kb on - strand at 1517.367 kb on + strand at 1517.367 kb on + strand at 1517.485 kb on + strand, within somA at 1517.488 kb on - strand, within somA at 1517.539 kb on - strand, within somA at 1517.632 kb on - strand, within somA at 1517.675 kb on - strand, within somA at 1517.687 kb on + strand, within somA at 1517.693 kb on - strand, within somA at 1517.711 kb on - strand, within somA at 1517.717 kb on + strand, within somA at 1517.781 kb on + strand, within somA at 1517.808 kb on + strand, within somA at 1517.816 kb on - strand, within somA at 1517.880 kb on + strand, within somA at 1517.969 kb on + strand, within somA at 1518.025 kb on - strand, within somA at 1518.053 kb on + strand, within somA at 1518.060 kb on + strand, within somA at 1518.110 kb on + strand, within somA at 1518.180 kb on + strand, within somA at 1518.268 kb on - strand, within somA at 1518.268 kb on - strand, within somA at 1518.274 kb on - strand, within somA at 1518.277 kb on + strand, within somA at 1518.281 kb on - strand, within somA at 1518.309 kb on - strand, within somA at 1518.364 kb on + strand, within somA at 1518.364 kb on + strand, within somA at 1518.364 kb on + strand, within somA at 1518.364 kb on + strand, within somA at 1518.364 kb on + strand, within somA at 1518.364 kb on + strand, within somA at 1518.364 kb on + strand, within somA at 1518.364 kb on + strand, within somA at 1518.372 kb on - strand, within somA at 1518.372 kb on - strand, within somA at 1518.372 kb on - strand, within somA at 1518.372 kb on - strand, within somA at 1518.474 kb on - strand, within somA at 1518.560 kb on + strand, within somA at 1518.560 kb on + strand, within somA at 1518.568 kb on - strand, within somA at 1518.568 kb on - strand, within somA at 1518.568 kb on - strand, within somA at 1518.854 kb on - strand at 1518.891 kb on + strand at 1518.992 kb on - strand at 1519.002 kb on - strand at 1519.143 kb on + strand, within Synpcc7942_1465 at 1519.222 kb on - strand, within Synpcc7942_1465 at 1519.256 kb on + strand, within Synpcc7942_1465 at 1519.482 kb on + strand at 1519.605 kb on - strand at 1519.610 kb on + strand at 1519.617 kb on + strand at 1519.681 kb on + strand at 1519.689 kb on - strand at 1519.689 kb on - strand at 1519.696 kb on + strand at 1519.698 kb on + strand at 1519.711 kb on - strand at 1519.711 kb on - strand at 1519.725 kb on + strand at 1519.733 kb on - strand at 1519.756 kb on - strand at 1519.756 kb on - strand at 1519.757 kb on - strand at 1519.759 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,516,268 + somB Synpcc7942_1463 0.55 -1.2 1,516,271 + somB Synpcc7942_1463 0.55 +1.6 1,516,329 + somB Synpcc7942_1463 0.59 -0.2 1,516,337 - somB Synpcc7942_1463 0.59 +2.7 1,516,337 - somB Synpcc7942_1463 0.59 -1.1 1,516,367 - somB Synpcc7942_1463 0.61 +0.5 1,516,390 - somB Synpcc7942_1463 0.63 +1.4 1,516,394 + somB Synpcc7942_1463 0.63 +1.5 1,516,402 - somB Synpcc7942_1463 0.63 -1.8 1,516,406 + -1.3 1,516,406 + somB Synpcc7942_1463 0.64 +2.6 1,516,407 - somB Synpcc7942_1463 0.64 +0.4 1,516,422 - somB Synpcc7942_1463 0.65 -1.2 1,516,444 + somB Synpcc7942_1463 0.66 +0.3 1,516,444 + somB Synpcc7942_1463 0.66 +2.2 1,516,452 - somB Synpcc7942_1463 0.66 +0.8 1,516,452 - somB Synpcc7942_1463 0.66 -1.4 1,516,461 + somB Synpcc7942_1463 0.67 -2.3 1,516,466 + somB Synpcc7942_1463 0.67 -1.3 1,516,466 + somB Synpcc7942_1463 0.67 +1.9 1,516,466 + somB Synpcc7942_1463 0.67 -0.2 1,516,466 + somB Synpcc7942_1463 0.67 -1.3 1,516,466 + somB Synpcc7942_1463 0.67 -1.7 1,516,466 + somB Synpcc7942_1463 0.67 -0.7 1,516,474 - somB Synpcc7942_1463 0.68 +1.2 1,516,474 - somB Synpcc7942_1463 0.68 +0.6 1,516,474 - somB Synpcc7942_1463 0.68 +1.8 1,516,474 - somB Synpcc7942_1463 0.68 +0.9 1,516,474 - somB Synpcc7942_1463 0.68 +1.0 1,516,474 - somB Synpcc7942_1463 0.68 +2.0 1,516,474 - somB Synpcc7942_1463 0.68 +1.3 1,516,474 - somB Synpcc7942_1463 0.68 -2.0 1,516,474 - somB Synpcc7942_1463 0.68 -0.8 1,516,486 + somB Synpcc7942_1463 0.68 -1.0 1,516,516 + somB Synpcc7942_1463 0.70 +0.3 1,516,567 + somB Synpcc7942_1463 0.73 +1.4 1,516,572 + somB Synpcc7942_1463 0.74 -1.4 1,516,572 + somB Synpcc7942_1463 0.74 +0.3 1,516,572 + somB Synpcc7942_1463 0.74 -1.7 1,516,577 + somB Synpcc7942_1463 0.74 +0.6 1,516,580 - somB Synpcc7942_1463 0.74 -0.4 1,516,580 - somB Synpcc7942_1463 0.74 +0.8 1,516,601 - somB Synpcc7942_1463 0.75 +2.6 1,516,610 + somB Synpcc7942_1463 0.76 +0.3 1,516,622 + somB Synpcc7942_1463 0.77 -0.5 1,516,628 + somB Synpcc7942_1463 0.77 -0.5 1,516,628 + somB Synpcc7942_1463 0.77 +0.3 1,516,638 - somB Synpcc7942_1463 0.78 +3.6 1,516,648 - somB Synpcc7942_1463 0.78 +0.5 1,516,672 + somB Synpcc7942_1463 0.80 -0.4 1,516,690 + somB Synpcc7942_1463 0.81 -0.3 1,516,710 + somB Synpcc7942_1463 0.82 +1.2 1,516,710 + somB Synpcc7942_1463 0.82 +1.4 1,516,715 + somB Synpcc7942_1463 0.82 -0.2 1,516,715 + somB Synpcc7942_1463 0.82 -1.1 1,516,715 + somB Synpcc7942_1463 0.82 +2.6 1,516,723 - somB Synpcc7942_1463 0.83 -1.0 1,516,737 + somB Synpcc7942_1463 0.84 +5.7 1,516,745 + somB Synpcc7942_1463 0.84 +3.7 1,516,771 + somB Synpcc7942_1463 0.86 -1.8 1,516,779 - somB Synpcc7942_1463 0.86 +1.2 1,516,781 + somB Synpcc7942_1463 0.87 -1.3 1,516,781 + somB Synpcc7942_1463 0.87 -1.4 1,516,783 + somB Synpcc7942_1463 0.87 -0.6 1,516,791 + somB Synpcc7942_1463 0.87 -2.9 1,516,804 + somB Synpcc7942_1463 0.88 -1.7 1,516,821 + somB Synpcc7942_1463 0.89 +3.1 1,516,824 + somB Synpcc7942_1463 0.89 -0.8 1,516,824 + somB Synpcc7942_1463 0.89 +0.0 1,516,894 + -1.8 1,516,894 + -0.1 1,516,910 - -0.5 1,516,929 + +1.7 1,516,937 - -0.7 1,516,937 - -0.1 1,517,003 - -1.9 1,517,025 - -1.5 1,517,042 - -1.7 1,517,050 + -2.0 1,517,058 - -0.4 1,517,058 - +1.5 1,517,094 - -1.1 1,517,096 + -0.5 1,517,312 + -1.5 1,517,327 - -3.6 1,517,367 + -2.4 1,517,367 + -2.9 1,517,485 + somA Synpcc7942_1464 0.16 -4.8 1,517,488 - somA Synpcc7942_1464 0.16 -1.7 1,517,539 - somA Synpcc7942_1464 0.19 -2.9 1,517,632 - somA Synpcc7942_1464 0.25 -2.1 1,517,675 - somA Synpcc7942_1464 0.27 -2.3 1,517,687 + somA Synpcc7942_1464 0.28 -4.1 1,517,693 - somA Synpcc7942_1464 0.29 -4.5 1,517,711 - somA Synpcc7942_1464 0.30 -2.9 1,517,717 + somA Synpcc7942_1464 0.30 -2.3 1,517,781 + somA Synpcc7942_1464 0.34 -1.3 1,517,808 + somA Synpcc7942_1464 0.36 -2.0 1,517,816 - somA Synpcc7942_1464 0.36 -3.7 1,517,880 + somA Synpcc7942_1464 0.40 -1.0 1,517,969 + somA Synpcc7942_1464 0.46 +0.7 1,518,025 - somA Synpcc7942_1464 0.49 -1.0 1,518,053 + somA Synpcc7942_1464 0.51 -4.1 1,518,060 + somA Synpcc7942_1464 0.52 -1.3 1,518,110 + somA Synpcc7942_1464 0.55 -2.9 1,518,180 + somA Synpcc7942_1464 0.59 -1.3 1,518,268 - somA Synpcc7942_1464 0.65 -1.9 1,518,268 - somA Synpcc7942_1464 0.65 -3.4 1,518,274 - somA Synpcc7942_1464 0.65 -1.7 1,518,277 + somA Synpcc7942_1464 0.65 -2.8 1,518,281 - somA Synpcc7942_1464 0.65 -2.5 1,518,309 - somA Synpcc7942_1464 0.67 -3.2 1,518,364 + somA Synpcc7942_1464 0.71 -3.3 1,518,364 + somA Synpcc7942_1464 0.71 -0.8 1,518,364 + somA Synpcc7942_1464 0.71 -2.0 1,518,364 + somA Synpcc7942_1464 0.71 -3.6 1,518,364 + somA Synpcc7942_1464 0.71 -4.2 1,518,364 + somA Synpcc7942_1464 0.71 -2.8 1,518,364 + somA Synpcc7942_1464 0.71 -1.9 1,518,364 + somA Synpcc7942_1464 0.71 -2.7 1,518,372 - somA Synpcc7942_1464 0.71 -1.7 1,518,372 - somA Synpcc7942_1464 0.71 -1.5 1,518,372 - somA Synpcc7942_1464 0.71 -0.5 1,518,372 - somA Synpcc7942_1464 0.71 -3.7 1,518,474 - somA Synpcc7942_1464 0.78 +1.4 1,518,560 + somA Synpcc7942_1464 0.83 -2.1 1,518,560 + somA Synpcc7942_1464 0.83 -5.4 1,518,568 - somA Synpcc7942_1464 0.83 -4.5 1,518,568 - somA Synpcc7942_1464 0.83 -2.9 1,518,568 - somA Synpcc7942_1464 0.83 -3.6 1,518,854 - -3.7 1,518,891 + +1.5 1,518,992 - -1.3 1,519,002 - -2.0 1,519,143 + Synpcc7942_1465 0.33 -1.5 1,519,222 - Synpcc7942_1465 0.50 +1.0 1,519,256 + Synpcc7942_1465 0.57 -1.8 1,519,482 + -0.3 1,519,605 - +1.2 1,519,610 + -0.5 1,519,617 + +3.7 1,519,681 + +0.5 1,519,689 - -1.1 1,519,689 - -0.9 1,519,696 + -0.4 1,519,698 + -1.6 1,519,711 - -0.6 1,519,711 - -0.3 1,519,725 + +2.1 1,519,733 - +0.2 1,519,756 - +0.8 1,519,756 - +5.3 1,519,757 - +0.6 1,519,759 + -0.3
Or see this region's nucleotide sequence