Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1436 and Synpcc7942_1437 are separated by 80 nucleotides Synpcc7942_1437 and nblC are separated by 231 nucleotides nblC and ndhD2 are separated by 138 nucleotides
Synpcc7942_1436: Synpcc7942_1436 - hypothetical protein, at 1,490,759 to 1,491,205
_1436
Synpcc7942_1437: Synpcc7942_1437 - hypothetical protein, at 1,491,286 to 1,491,564
_1437
Synpcc7942_1438: nblC - putative anti-sigma regulatory factor, at 1,491,796 to 1,492,290
nblC
Synpcc7942_1439: ndhD2 - NAD(P)H-quinone oxidoreductase subunit D, at 1,492,429 to 1,494,030
ndhD2
Position (kb)
1491
1492
1493 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1490.877 kb on + strand, within Synpcc7942_1436 at 1490.877 kb on + strand, within Synpcc7942_1436 at 1490.877 kb on + strand, within Synpcc7942_1436 at 1490.884 kb on + strand, within Synpcc7942_1436 at 1490.885 kb on - strand, within Synpcc7942_1436 at 1490.885 kb on - strand, within Synpcc7942_1436 at 1490.890 kb on + strand, within Synpcc7942_1436 at 1490.892 kb on - strand, within Synpcc7942_1436 at 1490.899 kb on - strand, within Synpcc7942_1436 at 1490.915 kb on - strand, within Synpcc7942_1436 at 1490.938 kb on - strand, within Synpcc7942_1436 at 1490.981 kb on - strand, within Synpcc7942_1436 at 1491.003 kb on - strand, within Synpcc7942_1436 at 1491.003 kb on - strand, within Synpcc7942_1436 at 1491.027 kb on - strand, within Synpcc7942_1436 at 1491.041 kb on + strand, within Synpcc7942_1436 at 1491.063 kb on - strand, within Synpcc7942_1436 at 1491.063 kb on - strand, within Synpcc7942_1436 at 1491.081 kb on - strand, within Synpcc7942_1436 at 1491.140 kb on - strand, within Synpcc7942_1436 at 1491.154 kb on + strand, within Synpcc7942_1436 at 1491.162 kb on - strand at 1491.197 kb on + strand at 1491.202 kb on + strand at 1491.202 kb on + strand at 1491.202 kb on + strand at 1491.209 kb on + strand at 1491.209 kb on + strand at 1491.209 kb on + strand at 1491.209 kb on + strand at 1491.210 kb on - strand at 1491.214 kb on + strand at 1491.214 kb on + strand at 1491.237 kb on + strand at 1491.248 kb on + strand at 1491.266 kb on - strand at 1491.275 kb on + strand at 1491.280 kb on + strand at 1491.283 kb on - strand at 1491.298 kb on + strand at 1491.299 kb on - strand at 1491.325 kb on - strand, within Synpcc7942_1437 at 1491.325 kb on - strand, within Synpcc7942_1437 at 1491.330 kb on - strand, within Synpcc7942_1437 at 1491.429 kb on - strand, within Synpcc7942_1437 at 1491.459 kb on - strand, within Synpcc7942_1437 at 1491.484 kb on + strand, within Synpcc7942_1437 at 1491.484 kb on + strand, within Synpcc7942_1437 at 1491.484 kb on + strand, within Synpcc7942_1437 at 1491.506 kb on + strand, within Synpcc7942_1437 at 1491.514 kb on - strand, within Synpcc7942_1437 at 1491.514 kb on - strand, within Synpcc7942_1437 at 1491.519 kb on - strand, within Synpcc7942_1437 at 1491.565 kb on + strand at 1491.565 kb on + strand at 1491.566 kb on - strand at 1491.573 kb on - strand at 1491.577 kb on + strand at 1491.577 kb on + strand at 1491.577 kb on + strand at 1491.599 kb on - strand at 1491.605 kb on + strand at 1491.609 kb on + strand at 1491.621 kb on - strand at 1491.623 kb on - strand at 1491.633 kb on + strand at 1491.659 kb on + strand at 1491.667 kb on - strand at 1491.667 kb on - strand at 1491.667 kb on - strand at 1491.745 kb on + strand at 1491.745 kb on + strand at 1491.753 kb on - strand at 1491.753 kb on - strand at 1491.753 kb on - strand at 1491.753 kb on - strand at 1491.753 kb on - strand at 1491.756 kb on + strand at 1491.764 kb on - strand at 1491.802 kb on - strand at 1491.866 kb on - strand, within nblC at 1491.869 kb on + strand, within nblC at 1491.893 kb on - strand at 1491.951 kb on + strand, within nblC at 1491.993 kb on - strand, within nblC at 1492.024 kb on - strand, within nblC at 1492.029 kb on - strand, within nblC at 1492.029 kb on - strand, within nblC at 1492.038 kb on + strand, within nblC at 1492.043 kb on - strand, within nblC at 1492.081 kb on + strand, within nblC at 1492.084 kb on - strand, within nblC at 1492.092 kb on + strand, within nblC at 1492.092 kb on + strand, within nblC at 1492.108 kb on + strand, within nblC at 1492.168 kb on + strand, within nblC at 1492.193 kb on + strand, within nblC at 1492.210 kb on + strand, within nblC at 1492.216 kb on + strand, within nblC at 1492.229 kb on - strand, within nblC at 1492.247 kb on + strand at 1492.256 kb on + strand at 1492.263 kb on + strand at 1492.263 kb on + strand at 1492.264 kb on - strand at 1492.279 kb on + strand at 1492.317 kb on - strand at 1492.321 kb on - strand at 1492.322 kb on - strand at 1492.325 kb on + strand at 1492.350 kb on - strand at 1492.355 kb on - strand at 1492.383 kb on - strand at 1492.387 kb on + strand at 1492.394 kb on - strand at 1492.394 kb on - strand at 1492.459 kb on - strand at 1492.468 kb on + strand at 1492.468 kb on + strand at 1492.502 kb on - strand at 1492.539 kb on + strand at 1492.542 kb on + strand at 1492.552 kb on + strand at 1492.574 kb on + strand at 1492.576 kb on + strand at 1492.582 kb on - strand at 1492.582 kb on - strand at 1492.674 kb on + strand, within ndhD2 at 1492.677 kb on - strand, within ndhD2 at 1492.677 kb on - strand, within ndhD2 at 1492.677 kb on - strand, within ndhD2 at 1492.677 kb on - strand, within ndhD2 at 1492.693 kb on + strand, within ndhD2 at 1492.694 kb on - strand, within ndhD2 at 1492.724 kb on + strand, within ndhD2 at 1492.724 kb on + strand, within ndhD2 at 1492.806 kb on - strand, within ndhD2 at 1492.830 kb on + strand, within ndhD2 at 1492.874 kb on - strand, within ndhD2 at 1492.874 kb on - strand at 1492.874 kb on - strand, within ndhD2 at 1492.874 kb on - strand at 1492.882 kb on + strand, within ndhD2 at 1492.899 kb on - strand, within ndhD2 at 1492.932 kb on - strand, within ndhD2 at 1492.936 kb on + strand, within ndhD2 at 1492.955 kb on + strand, within ndhD2 at 1492.963 kb on - strand, within ndhD2 at 1493.010 kb on + strand, within ndhD2 at 1493.010 kb on + strand, within ndhD2 at 1493.018 kb on - strand, within ndhD2 at 1493.034 kb on - strand, within ndhD2 at 1493.036 kb on + strand, within ndhD2 at 1493.107 kb on - strand, within ndhD2 at 1493.131 kb on + strand, within ndhD2 at 1493.143 kb on + strand, within ndhD2 at 1493.232 kb on + strand, within ndhD2 at 1493.263 kb on + strand, within ndhD2
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,490,877 + Synpcc7942_1436 0.26 -0.3 1,490,877 + Synpcc7942_1436 0.26 -1.4 1,490,877 + Synpcc7942_1436 0.26 -0.1 1,490,884 + Synpcc7942_1436 0.28 -0.8 1,490,885 - Synpcc7942_1436 0.28 +1.8 1,490,885 - Synpcc7942_1436 0.28 +2.7 1,490,890 + Synpcc7942_1436 0.29 +0.7 1,490,892 - Synpcc7942_1436 0.30 +0.3 1,490,899 - Synpcc7942_1436 0.31 +2.2 1,490,915 - Synpcc7942_1436 0.35 +1.6 1,490,938 - Synpcc7942_1436 0.40 -0.9 1,490,981 - Synpcc7942_1436 0.50 -0.4 1,491,003 - Synpcc7942_1436 0.55 -1.1 1,491,003 - Synpcc7942_1436 0.55 -0.2 1,491,027 - Synpcc7942_1436 0.60 +0.7 1,491,041 + Synpcc7942_1436 0.63 -1.5 1,491,063 - Synpcc7942_1436 0.68 -1.0 1,491,063 - Synpcc7942_1436 0.68 +1.7 1,491,081 - Synpcc7942_1436 0.72 -0.5 1,491,140 - Synpcc7942_1436 0.85 +2.2 1,491,154 + Synpcc7942_1436 0.88 -1.9 1,491,162 - +2.6 1,491,197 + -1.4 1,491,202 + +1.7 1,491,202 + -2.2 1,491,202 + -2.7 1,491,209 + -1.1 1,491,209 + -1.4 1,491,209 + -2.3 1,491,209 + +1.5 1,491,210 - +0.8 1,491,214 + -0.1 1,491,214 + -1.9 1,491,237 + +1.9 1,491,248 + +0.2 1,491,266 - -0.3 1,491,275 + -1.7 1,491,280 + -0.7 1,491,283 - -0.8 1,491,298 + +0.5 1,491,299 - -1.3 1,491,325 - Synpcc7942_1437 0.14 -1.1 1,491,325 - Synpcc7942_1437 0.14 -2.2 1,491,330 - Synpcc7942_1437 0.16 +1.2 1,491,429 - Synpcc7942_1437 0.51 +0.6 1,491,459 - Synpcc7942_1437 0.62 -3.2 1,491,484 + Synpcc7942_1437 0.71 -0.3 1,491,484 + Synpcc7942_1437 0.71 +0.0 1,491,484 + Synpcc7942_1437 0.71 -0.5 1,491,506 + Synpcc7942_1437 0.79 +1.5 1,491,514 - Synpcc7942_1437 0.82 +0.7 1,491,514 - Synpcc7942_1437 0.82 -0.1 1,491,519 - Synpcc7942_1437 0.84 +0.1 1,491,565 + -1.1 1,491,565 + -0.8 1,491,566 - +1.8 1,491,573 - +1.0 1,491,577 + -1.9 1,491,577 + +0.3 1,491,577 + -0.2 1,491,599 - -0.3 1,491,605 + -2.4 1,491,609 + -1.4 1,491,621 - +1.1 1,491,623 - -2.7 1,491,633 + +1.4 1,491,659 + -2.2 1,491,667 - -2.1 1,491,667 - +0.8 1,491,667 - -2.9 1,491,745 + -2.5 1,491,745 + +0.0 1,491,753 - -0.4 1,491,753 - -4.5 1,491,753 - -1.8 1,491,753 - -1.3 1,491,753 - -0.9 1,491,756 + -2.7 1,491,764 - -1.9 1,491,802 - -2.1 1,491,866 - nblC Synpcc7942_1438 0.14 -1.0 1,491,869 + nblC Synpcc7942_1438 0.15 -2.3 1,491,893 - -1.5 1,491,951 + nblC Synpcc7942_1438 0.31 -2.0 1,491,993 - nblC Synpcc7942_1438 0.40 -4.4 1,492,024 - nblC Synpcc7942_1438 0.46 -2.8 1,492,029 - nblC Synpcc7942_1438 0.47 -2.9 1,492,029 - nblC Synpcc7942_1438 0.47 -1.7 1,492,038 + nblC Synpcc7942_1438 0.49 -2.0 1,492,043 - nblC Synpcc7942_1438 0.50 +0.6 1,492,081 + nblC Synpcc7942_1438 0.58 -2.4 1,492,084 - nblC Synpcc7942_1438 0.58 -3.0 1,492,092 + nblC Synpcc7942_1438 0.60 -1.9 1,492,092 + nblC Synpcc7942_1438 0.60 -2.0 1,492,108 + nblC Synpcc7942_1438 0.63 -2.4 1,492,168 + nblC Synpcc7942_1438 0.75 -2.0 1,492,193 + nblC Synpcc7942_1438 0.80 -3.0 1,492,210 + nblC Synpcc7942_1438 0.84 -1.1 1,492,216 + nblC Synpcc7942_1438 0.85 -3.9 1,492,229 - nblC Synpcc7942_1438 0.87 -1.7 1,492,247 + -5.3 1,492,256 + -4.6 1,492,263 + +3.4 1,492,263 + -1.9 1,492,264 - -4.5 1,492,279 + -1.1 1,492,317 - -1.8 1,492,321 - -1.0 1,492,322 - -2.8 1,492,325 + -2.3 1,492,350 - -1.4 1,492,355 - -2.2 1,492,383 - -3.5 1,492,387 + +0.2 1,492,394 - -2.2 1,492,394 - -2.3 1,492,459 - +0.7 1,492,468 + -0.3 1,492,468 + -1.4 1,492,502 - -1.0 1,492,539 + -3.0 1,492,542 + -2.5 1,492,552 + -1.1 1,492,574 + -2.8 1,492,576 + -2.4 1,492,582 - -2.5 1,492,582 - -1.5 1,492,674 + ndhD2 Synpcc7942_1439 0.15 -1.2 1,492,677 - ndhD2 Synpcc7942_1439 0.15 -3.5 1,492,677 - ndhD2 Synpcc7942_1439 0.15 -3.4 1,492,677 - ndhD2 Synpcc7942_1439 0.15 -4.0 1,492,677 - ndhD2 Synpcc7942_1439 0.15 -3.0 1,492,693 + ndhD2 Synpcc7942_1439 0.16 -2.7 1,492,694 - ndhD2 Synpcc7942_1439 0.17 -1.8 1,492,724 + ndhD2 Synpcc7942_1439 0.18 -3.9 1,492,724 + ndhD2 Synpcc7942_1439 0.18 -0.9 1,492,806 - ndhD2 Synpcc7942_1439 0.24 -2.9 1,492,830 + ndhD2 Synpcc7942_1439 0.25 -1.7 1,492,874 - ndhD2 Synpcc7942_1439 0.28 -2.3 1,492,874 - -1.7 1,492,874 - ndhD2 Synpcc7942_1439 0.28 -2.3 1,492,874 - -2.0 1,492,882 + ndhD2 Synpcc7942_1439 0.28 -3.2 1,492,899 - ndhD2 Synpcc7942_1439 0.29 -3.3 1,492,932 - ndhD2 Synpcc7942_1439 0.31 -3.5 1,492,936 + ndhD2 Synpcc7942_1439 0.32 -2.9 1,492,955 + ndhD2 Synpcc7942_1439 0.33 -1.5 1,492,963 - ndhD2 Synpcc7942_1439 0.33 -2.5 1,493,010 + ndhD2 Synpcc7942_1439 0.36 -5.1 1,493,010 + ndhD2 Synpcc7942_1439 0.36 -3.4 1,493,018 - ndhD2 Synpcc7942_1439 0.37 -3.7 1,493,034 - ndhD2 Synpcc7942_1439 0.38 -1.0 1,493,036 + ndhD2 Synpcc7942_1439 0.38 -3.2 1,493,107 - ndhD2 Synpcc7942_1439 0.42 -2.4 1,493,131 + ndhD2 Synpcc7942_1439 0.44 -3.1 1,493,143 + ndhD2 Synpcc7942_1439 0.45 -2.7 1,493,232 + ndhD2 Synpcc7942_1439 0.50 -1.9 1,493,263 + ndhD2 Synpcc7942_1439 0.52 -2.0
Or see this region's nucleotide sequence