Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argC and Synpcc7942_1434 are separated by 213 nucleotides Synpcc7942_1434 and menD are separated by 120 nucleotides
Synpcc7942_1433: argC - N-acetyl-gamma-glutamyl-phosphate reductase, at 1,486,983 to 1,488,041
argC
Synpcc7942_1434: Synpcc7942_1434 - hypothetical protein, at 1,488,255 to 1,488,854
_1434
Synpcc7942_1435: menD - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylic acid synthase/2-oxoglutarate decarboxylase, at 1,488,975 to 1,490,723
menD
Position (kb)
1488
1489 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1488.034 kb on + strand at 1488.035 kb on + strand at 1488.035 kb on + strand at 1488.035 kb on + strand at 1488.057 kb on + strand at 1488.057 kb on + strand at 1488.057 kb on + strand at 1488.057 kb on + strand at 1488.065 kb on - strand at 1488.065 kb on - strand at 1488.065 kb on - strand at 1488.068 kb on - strand at 1488.133 kb on - strand at 1488.136 kb on + strand at 1488.161 kb on + strand at 1488.164 kb on - strand at 1488.167 kb on - strand at 1488.169 kb on - strand at 1488.276 kb on - strand at 1488.355 kb on + strand, within Synpcc7942_1434 at 1488.395 kb on - strand, within Synpcc7942_1434 at 1488.434 kb on + strand, within Synpcc7942_1434 at 1488.448 kb on - strand, within Synpcc7942_1434 at 1488.461 kb on - strand, within Synpcc7942_1434 at 1488.484 kb on - strand, within Synpcc7942_1434 at 1488.500 kb on + strand, within Synpcc7942_1434 at 1488.508 kb on - strand, within Synpcc7942_1434 at 1488.533 kb on + strand, within Synpcc7942_1434 at 1488.581 kb on + strand, within Synpcc7942_1434 at 1488.581 kb on + strand, within Synpcc7942_1434 at 1488.584 kb on - strand, within Synpcc7942_1434 at 1488.589 kb on - strand, within Synpcc7942_1434 at 1488.625 kb on - strand, within Synpcc7942_1434 at 1488.648 kb on + strand, within Synpcc7942_1434 at 1488.654 kb on - strand at 1488.656 kb on - strand, within Synpcc7942_1434 at 1488.688 kb on - strand, within Synpcc7942_1434 at 1488.707 kb on - strand, within Synpcc7942_1434 at 1488.712 kb on - strand, within Synpcc7942_1434 at 1488.742 kb on - strand, within Synpcc7942_1434 at 1488.795 kb on - strand at 1488.858 kb on + strand at 1488.901 kb on - strand at 1488.983 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,488,034 + -1.3 1,488,035 + +1.8 1,488,035 + -2.4 1,488,035 + -0.6 1,488,057 + -0.5 1,488,057 + +1.0 1,488,057 + -2.2 1,488,057 + -1.7 1,488,065 - +0.1 1,488,065 - +1.0 1,488,065 - -0.5 1,488,068 - -0.4 1,488,133 - -2.8 1,488,136 + -2.5 1,488,161 + +1.0 1,488,164 - -1.0 1,488,167 - -1.8 1,488,169 - +1.0 1,488,276 - +2.0 1,488,355 + Synpcc7942_1434 0.17 +1.7 1,488,395 - Synpcc7942_1434 0.23 +2.4 1,488,434 + Synpcc7942_1434 0.30 -0.5 1,488,448 - Synpcc7942_1434 0.32 +3.5 1,488,461 - Synpcc7942_1434 0.34 +3.9 1,488,484 - Synpcc7942_1434 0.38 +4.1 1,488,500 + Synpcc7942_1434 0.41 +2.8 1,488,508 - Synpcc7942_1434 0.42 +3.3 1,488,533 + Synpcc7942_1434 0.46 +2.8 1,488,581 + Synpcc7942_1434 0.54 +0.6 1,488,581 + Synpcc7942_1434 0.54 +2.2 1,488,584 - Synpcc7942_1434 0.55 +2.0 1,488,589 - Synpcc7942_1434 0.56 +2.9 1,488,625 - Synpcc7942_1434 0.62 +0.3 1,488,648 + Synpcc7942_1434 0.66 +2.1 1,488,654 - +2.5 1,488,656 - Synpcc7942_1434 0.67 +1.5 1,488,688 - Synpcc7942_1434 0.72 +3.0 1,488,707 - Synpcc7942_1434 0.75 -0.5 1,488,712 - Synpcc7942_1434 0.76 -0.3 1,488,742 - Synpcc7942_1434 0.81 +1.7 1,488,795 - +0.5 1,488,858 + +0.9 1,488,901 - +2.9 1,488,983 + +3.3
Or see this region's nucleotide sequence