Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuA and Synpcc7942_1411 are separated by 79 nucleotides Synpcc7942_1411 and Synpcc7942_1412 are separated by 9 nucleotides Synpcc7942_1412 and Synpcc7942_1413 are separated by 123 nucleotides
Synpcc7942_1410: leuA - 2-isopropylmalate synthase, at 1,461,949 to 1,463,571
leuA
Synpcc7942_1411: Synpcc7942_1411 - hypothetical protein, at 1,463,651 to 1,464,358
_1411
Synpcc7942_1412: Synpcc7942_1412 - hypothetical protein, at 1,464,368 to 1,464,817
_1412
Synpcc7942_1413: Synpcc7942_1413 - hypothetical protein, at 1,464,941 to 1,465,696
_1413
Position (kb)
1463
1464
1465 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1463.629 kb on + strand at 1463.639 kb on + strand at 1463.666 kb on - strand at 1463.705 kb on + strand at 1463.705 kb on + strand at 1463.713 kb on - strand at 1463.719 kb on + strand at 1463.720 kb on + strand at 1463.720 kb on + strand at 1463.727 kb on - strand, within Synpcc7942_1411 at 1463.731 kb on + strand, within Synpcc7942_1411 at 1463.733 kb on - strand, within Synpcc7942_1411 at 1463.749 kb on - strand, within Synpcc7942_1411 at 1463.756 kb on + strand, within Synpcc7942_1411 at 1463.764 kb on - strand, within Synpcc7942_1411 at 1463.789 kb on + strand, within Synpcc7942_1411 at 1463.797 kb on - strand, within Synpcc7942_1411 at 1463.818 kb on + strand, within Synpcc7942_1411 at 1463.854 kb on - strand, within Synpcc7942_1411 at 1463.891 kb on - strand, within Synpcc7942_1411 at 1463.891 kb on - strand, within Synpcc7942_1411 at 1463.896 kb on - strand, within Synpcc7942_1411 at 1463.945 kb on + strand, within Synpcc7942_1411 at 1463.958 kb on - strand, within Synpcc7942_1411 at 1464.007 kb on - strand, within Synpcc7942_1411 at 1464.017 kb on - strand, within Synpcc7942_1411 at 1464.042 kb on - strand, within Synpcc7942_1411 at 1464.049 kb on + strand, within Synpcc7942_1411 at 1464.049 kb on + strand, within Synpcc7942_1411 at 1464.049 kb on - strand, within Synpcc7942_1411 at 1464.051 kb on + strand, within Synpcc7942_1411 at 1464.051 kb on + strand, within Synpcc7942_1411 at 1464.059 kb on - strand, within Synpcc7942_1411 at 1464.119 kb on + strand, within Synpcc7942_1411 at 1464.119 kb on + strand, within Synpcc7942_1411 at 1464.142 kb on - strand, within Synpcc7942_1411 at 1464.164 kb on + strand, within Synpcc7942_1411 at 1464.208 kb on + strand, within Synpcc7942_1411 at 1464.208 kb on + strand, within Synpcc7942_1411 at 1464.209 kb on + strand, within Synpcc7942_1411 at 1464.211 kb on - strand, within Synpcc7942_1411 at 1464.216 kb on - strand, within Synpcc7942_1411 at 1464.216 kb on - strand, within Synpcc7942_1411 at 1464.290 kb on + strand at 1464.332 kb on - strand at 1464.360 kb on + strand at 1464.368 kb on - strand at 1464.371 kb on + strand at 1464.373 kb on - strand at 1464.395 kb on - strand at 1464.397 kb on + strand at 1464.453 kb on + strand at 1464.479 kb on + strand, within Synpcc7942_1412 at 1464.530 kb on - strand, within Synpcc7942_1412 at 1464.535 kb on - strand, within Synpcc7942_1412 at 1464.547 kb on + strand at 1464.553 kb on - strand, within Synpcc7942_1412 at 1464.577 kb on + strand, within Synpcc7942_1412 at 1464.611 kb on + strand, within Synpcc7942_1412 at 1464.672 kb on - strand, within Synpcc7942_1412 at 1464.673 kb on - strand, within Synpcc7942_1412 at 1464.690 kb on + strand, within Synpcc7942_1412 at 1464.695 kb on + strand, within Synpcc7942_1412 at 1464.695 kb on + strand, within Synpcc7942_1412 at 1464.695 kb on + strand, within Synpcc7942_1412 at 1464.697 kb on + strand, within Synpcc7942_1412 at 1464.703 kb on - strand, within Synpcc7942_1412 at 1464.703 kb on - strand, within Synpcc7942_1412 at 1464.703 kb on - strand, within Synpcc7942_1412 at 1464.705 kb on + strand, within Synpcc7942_1412 at 1464.719 kb on - strand, within Synpcc7942_1412 at 1464.719 kb on - strand, within Synpcc7942_1412 at 1464.735 kb on + strand, within Synpcc7942_1412 at 1464.780 kb on + strand at 1464.813 kb on + strand at 1464.821 kb on - strand at 1464.831 kb on - strand at 1464.857 kb on - strand at 1464.857 kb on - strand at 1464.857 kb on - strand at 1464.871 kb on + strand at 1464.871 kb on + strand at 1464.879 kb on - strand at 1464.881 kb on + strand at 1464.905 kb on - strand at 1464.906 kb on + strand at 1464.907 kb on + strand at 1464.925 kb on - strand at 1465.007 kb on + strand at 1465.010 kb on + strand at 1465.027 kb on - strand, within Synpcc7942_1413 at 1465.035 kb on + strand, within Synpcc7942_1413 at 1465.035 kb on + strand, within Synpcc7942_1413 at 1465.043 kb on - strand at 1465.052 kb on + strand, within Synpcc7942_1413 at 1465.090 kb on - strand, within Synpcc7942_1413 at 1465.092 kb on + strand, within Synpcc7942_1413 at 1465.157 kb on - strand, within Synpcc7942_1413 at 1465.163 kb on + strand, within Synpcc7942_1413 at 1465.179 kb on - strand, within Synpcc7942_1413 at 1465.181 kb on + strand, within Synpcc7942_1413 at 1465.223 kb on + strand, within Synpcc7942_1413 at 1465.264 kb on - strand, within Synpcc7942_1413 at 1465.315 kb on + strand, within Synpcc7942_1413 at 1465.315 kb on + strand at 1465.315 kb on + strand at 1465.329 kb on + strand, within Synpcc7942_1413 at 1465.338 kb on - strand, within Synpcc7942_1413 at 1465.349 kb on - strand, within Synpcc7942_1413 at 1465.351 kb on + strand, within Synpcc7942_1413
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,463,629 + -1.9 1,463,639 + -0.2 1,463,666 - +0.3 1,463,705 + -3.3 1,463,705 + -0.2 1,463,713 - -2.0 1,463,719 + -2.3 1,463,720 + -3.5 1,463,720 + -1.9 1,463,727 - Synpcc7942_1411 0.11 -3.3 1,463,731 + Synpcc7942_1411 0.11 -1.3 1,463,733 - Synpcc7942_1411 0.12 -2.7 1,463,749 - Synpcc7942_1411 0.14 -2.6 1,463,756 + Synpcc7942_1411 0.15 -1.8 1,463,764 - Synpcc7942_1411 0.16 -2.1 1,463,789 + Synpcc7942_1411 0.19 -1.5 1,463,797 - Synpcc7942_1411 0.21 +1.5 1,463,818 + Synpcc7942_1411 0.24 -2.4 1,463,854 - Synpcc7942_1411 0.29 -1.7 1,463,891 - Synpcc7942_1411 0.34 -3.2 1,463,891 - Synpcc7942_1411 0.34 -3.3 1,463,896 - Synpcc7942_1411 0.35 -3.7 1,463,945 + Synpcc7942_1411 0.42 -1.8 1,463,958 - Synpcc7942_1411 0.43 -3.8 1,464,007 - Synpcc7942_1411 0.50 -2.0 1,464,017 - Synpcc7942_1411 0.52 -4.4 1,464,042 - Synpcc7942_1411 0.55 -4.2 1,464,049 + Synpcc7942_1411 0.56 -2.2 1,464,049 + Synpcc7942_1411 0.56 -1.3 1,464,049 - Synpcc7942_1411 0.56 +2.2 1,464,051 + Synpcc7942_1411 0.56 -2.9 1,464,051 + Synpcc7942_1411 0.56 -3.4 1,464,059 - Synpcc7942_1411 0.58 -0.3 1,464,119 + Synpcc7942_1411 0.66 -1.3 1,464,119 + Synpcc7942_1411 0.66 -1.9 1,464,142 - Synpcc7942_1411 0.69 -3.3 1,464,164 + Synpcc7942_1411 0.72 -1.3 1,464,208 + Synpcc7942_1411 0.79 -0.3 1,464,208 + Synpcc7942_1411 0.79 -2.0 1,464,209 + Synpcc7942_1411 0.79 -2.3 1,464,211 - Synpcc7942_1411 0.79 -0.0 1,464,216 - Synpcc7942_1411 0.80 -2.0 1,464,216 - Synpcc7942_1411 0.80 -2.3 1,464,290 + -2.2 1,464,332 - -1.7 1,464,360 + +0.7 1,464,368 - +0.9 1,464,371 + -1.1 1,464,373 - -0.6 1,464,395 - +3.1 1,464,397 + +3.4 1,464,453 + +2.6 1,464,479 + Synpcc7942_1412 0.25 +2.2 1,464,530 - Synpcc7942_1412 0.36 -1.5 1,464,535 - Synpcc7942_1412 0.37 -0.8 1,464,547 + +0.0 1,464,553 - Synpcc7942_1412 0.41 -2.7 1,464,577 + Synpcc7942_1412 0.46 +0.5 1,464,611 + Synpcc7942_1412 0.54 -0.9 1,464,672 - Synpcc7942_1412 0.68 -2.3 1,464,673 - Synpcc7942_1412 0.68 +2.2 1,464,690 + Synpcc7942_1412 0.72 +1.0 1,464,695 + Synpcc7942_1412 0.73 +0.4 1,464,695 + Synpcc7942_1412 0.73 +2.6 1,464,695 + Synpcc7942_1412 0.73 -2.4 1,464,697 + Synpcc7942_1412 0.73 +1.0 1,464,703 - Synpcc7942_1412 0.74 -2.8 1,464,703 - Synpcc7942_1412 0.74 -1.5 1,464,703 - Synpcc7942_1412 0.74 -1.5 1,464,705 + Synpcc7942_1412 0.75 +2.6 1,464,719 - Synpcc7942_1412 0.78 -1.0 1,464,719 - Synpcc7942_1412 0.78 +2.0 1,464,735 + Synpcc7942_1412 0.82 -2.2 1,464,780 + +1.0 1,464,813 + -0.7 1,464,821 - -2.0 1,464,831 - +0.6 1,464,857 - -0.0 1,464,857 - -0.8 1,464,857 - -1.7 1,464,871 + +4.3 1,464,871 + -3.2 1,464,879 - +0.6 1,464,881 + -0.2 1,464,905 - -2.4 1,464,906 + -1.4 1,464,907 + +0.6 1,464,925 - -2.7 1,465,007 + -3.5 1,465,010 + -1.4 1,465,027 - Synpcc7942_1413 0.11 -2.0 1,465,035 + Synpcc7942_1413 0.12 -0.3 1,465,035 + Synpcc7942_1413 0.12 -1.0 1,465,043 - -0.5 1,465,052 + Synpcc7942_1413 0.15 -1.7 1,465,090 - Synpcc7942_1413 0.20 -1.8 1,465,092 + Synpcc7942_1413 0.20 -2.9 1,465,157 - Synpcc7942_1413 0.29 -2.4 1,465,163 + Synpcc7942_1413 0.29 +1.7 1,465,179 - Synpcc7942_1413 0.31 -1.5 1,465,181 + Synpcc7942_1413 0.32 -0.4 1,465,223 + Synpcc7942_1413 0.37 -1.8 1,465,264 - Synpcc7942_1413 0.43 -3.1 1,465,315 + Synpcc7942_1413 0.49 -1.3 1,465,315 + -0.8 1,465,315 + -0.3 1,465,329 + Synpcc7942_1413 0.51 -2.0 1,465,338 - Synpcc7942_1413 0.53 -2.0 1,465,349 - Synpcc7942_1413 0.54 -1.3 1,465,351 + Synpcc7942_1413 0.54 -1.9
Or see this region's nucleotide sequence