Experiment: Flask grown to static phase (untill yellowing)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1400 and Synpcc7942_1401 are separated by 350 nucleotides Synpcc7942_1401 and Synpcc7942_1402 are separated by 212 nucleotides Synpcc7942_1402 and Synpcc7942_1403 are separated by 16 nucleotides Synpcc7942_1403 and Synpcc7942_1404 are separated by 25 nucleotides
Synpcc7942_1400: Synpcc7942_1400 - Endo-1,4-beta-xylanase, at 1,451,255 to 1,452,376
_1400
Synpcc7942_1401: Synpcc7942_1401 - hypothetical protein, at 1,452,727 to 1,453,104
_1401
Synpcc7942_1402: Synpcc7942_1402 - hypothetical protein, at 1,453,317 to 1,454,576
_1402
Synpcc7942_1403: Synpcc7942_1403 - Hydroxyacylglutathione hydrolase, at 1,454,593 to 1,455,369
_1403
Synpcc7942_1404: Synpcc7942_1404 - two component transcriptional regulator, winged helix family, at 1,455,395 to 1,456,084
_1404
Position (kb)
1453
1454
1455 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1452.361 kb on + strand at 1452.369 kb on - strand at 1452.384 kb on - strand at 1452.397 kb on - strand at 1452.404 kb on - strand at 1452.760 kb on + strand at 1453.126 kb on - strand at 1453.158 kb on + strand at 1453.163 kb on - strand at 1453.166 kb on - strand at 1453.199 kb on - strand at 1453.209 kb on - strand at 1453.266 kb on - strand at 1453.295 kb on - strand at 1453.361 kb on + strand at 1453.487 kb on - strand, within Synpcc7942_1402 at 1453.679 kb on - strand, within Synpcc7942_1402 at 1453.689 kb on - strand, within Synpcc7942_1402 at 1453.689 kb on - strand, within Synpcc7942_1402 at 1453.844 kb on + strand, within Synpcc7942_1402 at 1453.844 kb on + strand, within Synpcc7942_1402 at 1453.852 kb on - strand, within Synpcc7942_1402 at 1453.854 kb on - strand, within Synpcc7942_1402 at 1454.033 kb on - strand, within Synpcc7942_1402 at 1454.059 kb on + strand, within Synpcc7942_1402 at 1454.210 kb on + strand, within Synpcc7942_1402 at 1454.286 kb on - strand, within Synpcc7942_1402 at 1454.297 kb on - strand, within Synpcc7942_1402 at 1454.297 kb on - strand, within Synpcc7942_1402 at 1454.297 kb on - strand, within Synpcc7942_1402 at 1454.356 kb on - strand, within Synpcc7942_1402 at 1454.356 kb on - strand, within Synpcc7942_1402 at 1454.451 kb on + strand at 1454.461 kb on + strand at 1454.508 kb on + strand at 1454.533 kb on + strand at 1454.533 kb on + strand at 1454.533 kb on + strand at 1454.533 kb on + strand at 1454.533 kb on + strand at 1454.541 kb on - strand at 1454.541 kb on - strand at 1454.541 kb on - strand at 1454.541 kb on - strand at 1454.541 kb on - strand at 1454.543 kb on - strand at 1454.570 kb on + strand at 1454.577 kb on - strand at 1454.586 kb on - strand at 1454.591 kb on + strand at 1454.599 kb on - strand at 1454.637 kb on - strand at 1454.637 kb on - strand at 1454.644 kb on - strand at 1454.662 kb on + strand at 1454.670 kb on - strand at 1454.700 kb on - strand, within Synpcc7942_1403 at 1454.722 kb on + strand, within Synpcc7942_1403 at 1454.726 kb on + strand, within Synpcc7942_1403 at 1454.747 kb on - strand, within Synpcc7942_1403 at 1454.816 kb on + strand, within Synpcc7942_1403 at 1454.827 kb on + strand, within Synpcc7942_1403 at 1454.834 kb on + strand, within Synpcc7942_1403 at 1454.836 kb on + strand, within Synpcc7942_1403 at 1454.846 kb on + strand, within Synpcc7942_1403 at 1454.861 kb on - strand, within Synpcc7942_1403 at 1454.871 kb on - strand, within Synpcc7942_1403 at 1454.886 kb on + strand, within Synpcc7942_1403 at 1454.960 kb on + strand, within Synpcc7942_1403 at 1454.965 kb on + strand, within Synpcc7942_1403 at 1454.973 kb on - strand, within Synpcc7942_1403 at 1454.975 kb on + strand, within Synpcc7942_1403 at 1454.976 kb on + strand, within Synpcc7942_1403 at 1454.977 kb on + strand, within Synpcc7942_1403 at 1454.983 kb on - strand, within Synpcc7942_1403 at 1454.983 kb on - strand, within Synpcc7942_1403 at 1455.007 kb on + strand, within Synpcc7942_1403 at 1455.021 kb on + strand, within Synpcc7942_1403 at 1455.021 kb on + strand, within Synpcc7942_1403 at 1455.029 kb on - strand, within Synpcc7942_1403 at 1455.035 kb on - strand, within Synpcc7942_1403 at 1455.040 kb on - strand, within Synpcc7942_1403 at 1455.051 kb on - strand, within Synpcc7942_1403 at 1455.073 kb on - strand, within Synpcc7942_1403 at 1455.090 kb on + strand, within Synpcc7942_1403 at 1455.110 kb on + strand at 1455.118 kb on - strand, within Synpcc7942_1403 at 1455.139 kb on + strand, within Synpcc7942_1403 at 1455.147 kb on + strand, within Synpcc7942_1403 at 1455.147 kb on + strand, within Synpcc7942_1403 at 1455.225 kb on + strand, within Synpcc7942_1403 at 1455.227 kb on + strand, within Synpcc7942_1403 at 1455.233 kb on - strand, within Synpcc7942_1403 at 1455.233 kb on - strand, within Synpcc7942_1403 at 1455.237 kb on + strand, within Synpcc7942_1403 at 1455.247 kb on - strand, within Synpcc7942_1403 at 1455.272 kb on + strand, within Synpcc7942_1403 at 1455.282 kb on + strand, within Synpcc7942_1403 at 1455.289 kb on - strand, within Synpcc7942_1403 at 1455.304 kb on - strand at 1455.306 kb on + strand at 1455.309 kb on - strand at 1455.314 kb on - strand at 1455.345 kb on - strand at 1455.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown to static phase (untill yellowing) remove 1,452,361 + -0.4 1,452,369 - +3.3 1,452,384 - +0.4 1,452,397 - -0.9 1,452,404 - +1.2 1,452,760 + -1.7 1,453,126 - -5.0 1,453,158 + -3.2 1,453,163 - +2.5 1,453,166 - -0.1 1,453,199 - -1.2 1,453,209 - -0.1 1,453,266 - +1.6 1,453,295 - -0.7 1,453,361 + +0.8 1,453,487 - Synpcc7942_1402 0.13 +0.6 1,453,679 - Synpcc7942_1402 0.29 -1.5 1,453,689 - Synpcc7942_1402 0.30 -2.5 1,453,689 - Synpcc7942_1402 0.30 -0.1 1,453,844 + Synpcc7942_1402 0.42 -1.7 1,453,844 + Synpcc7942_1402 0.42 -0.3 1,453,852 - Synpcc7942_1402 0.42 -2.4 1,453,854 - Synpcc7942_1402 0.43 -1.7 1,454,033 - Synpcc7942_1402 0.57 -2.7 1,454,059 + Synpcc7942_1402 0.59 +1.3 1,454,210 + Synpcc7942_1402 0.71 +0.5 1,454,286 - Synpcc7942_1402 0.77 -3.5 1,454,297 - Synpcc7942_1402 0.78 +1.6 1,454,297 - Synpcc7942_1402 0.78 +1.2 1,454,297 - Synpcc7942_1402 0.78 +0.2 1,454,356 - Synpcc7942_1402 0.82 +0.5 1,454,356 - Synpcc7942_1402 0.82 -3.0 1,454,451 + -1.3 1,454,461 + +0.6 1,454,508 + -3.1 1,454,533 + +1.1 1,454,533 + -3.2 1,454,533 + +1.4 1,454,533 + -2.9 1,454,533 + -3.4 1,454,541 - -2.0 1,454,541 - -1.1 1,454,541 - +1.0 1,454,541 - -1.4 1,454,541 - -1.8 1,454,543 - -1.0 1,454,570 + +1.1 1,454,577 - +0.4 1,454,586 - +0.9 1,454,591 + -0.9 1,454,599 - -1.7 1,454,637 - +0.4 1,454,637 - +1.4 1,454,644 - +3.2 1,454,662 + -1.7 1,454,670 - +0.8 1,454,700 - Synpcc7942_1403 0.14 +0.5 1,454,722 + Synpcc7942_1403 0.17 +1.9 1,454,726 + Synpcc7942_1403 0.17 -2.6 1,454,747 - Synpcc7942_1403 0.20 -1.4 1,454,816 + Synpcc7942_1403 0.29 +1.5 1,454,827 + Synpcc7942_1403 0.30 +1.9 1,454,834 + Synpcc7942_1403 0.31 +0.2 1,454,836 + Synpcc7942_1403 0.31 +1.5 1,454,846 + Synpcc7942_1403 0.33 +2.2 1,454,861 - Synpcc7942_1403 0.34 -2.6 1,454,871 - Synpcc7942_1403 0.36 -0.5 1,454,886 + Synpcc7942_1403 0.38 +1.2 1,454,960 + Synpcc7942_1403 0.47 +0.6 1,454,965 + Synpcc7942_1403 0.48 +2.7 1,454,973 - Synpcc7942_1403 0.49 -0.4 1,454,975 + Synpcc7942_1403 0.49 +1.0 1,454,976 + Synpcc7942_1403 0.49 -1.8 1,454,977 + Synpcc7942_1403 0.49 -0.3 1,454,983 - Synpcc7942_1403 0.50 -1.2 1,454,983 - Synpcc7942_1403 0.50 +0.4 1,455,007 + Synpcc7942_1403 0.53 -1.7 1,455,021 + Synpcc7942_1403 0.55 -0.8 1,455,021 + Synpcc7942_1403 0.55 -1.5 1,455,029 - Synpcc7942_1403 0.56 -0.6 1,455,035 - Synpcc7942_1403 0.57 -2.1 1,455,040 - Synpcc7942_1403 0.58 +0.6 1,455,051 - Synpcc7942_1403 0.59 +1.1 1,455,073 - Synpcc7942_1403 0.62 -0.7 1,455,090 + Synpcc7942_1403 0.64 -1.1 1,455,110 + -1.9 1,455,118 - Synpcc7942_1403 0.68 +0.0 1,455,139 + Synpcc7942_1403 0.70 +0.9 1,455,147 + Synpcc7942_1403 0.71 -0.3 1,455,147 + Synpcc7942_1403 0.71 -0.9 1,455,225 + Synpcc7942_1403 0.81 +1.9 1,455,227 + Synpcc7942_1403 0.82 -2.4 1,455,233 - Synpcc7942_1403 0.82 +0.8 1,455,233 - Synpcc7942_1403 0.82 -1.8 1,455,237 + Synpcc7942_1403 0.83 +1.0 1,455,247 - Synpcc7942_1403 0.84 +2.6 1,455,272 + Synpcc7942_1403 0.87 -0.8 1,455,282 + Synpcc7942_1403 0.89 -1.0 1,455,289 - Synpcc7942_1403 0.90 -3.8 1,455,304 - -0.8 1,455,306 + -1.7 1,455,309 - +2.7 1,455,314 - +1.7 1,455,345 - +1.0 1,455,364 - +0.9
Or see this region's nucleotide sequence